ISLpn12

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_006368 ND Legionella pneumophila
Legionella pneumophila Paris
Legionella pneumophila Corby
Legionella pneumophila Paris plasmid pLPP
DNA section
IS Length : 1996 bp

Ends


IR Length : 41/53

IRL : TGTCGTTGCTGAATATAAACTGATCCCCTTTGCCGACTGAAAATTGATCC
IRR : TGTCAATGCCGATTGAAAATTGATCCTTTTGCCAGATGAAAATTGATCCC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAATTACTGACAATAGTGCTTATATT7
CAGATAAGTATTTGCCAGCAAGAATT6
ATTGTCCTTATTTCTCATCAATGACA6
ACTTTCCGATTAAATGAATGTTCA4
ATTGTCCTTATTTCTCATCAATGACA6

DNA sequence

TGTCGTTGCTGAATATAAACTGATCCCCTTTGCCGACTGAAAATTGATCCCCCTACTATAGTTTTCTTCAGGAGAATAAATTCTGGAGGAGGGAATGATT
CAATTAGAGGAGTCAAAAATGATTCATATTTTGTATAAGCAGGGATACAGTAAAAAAGCGATTGCTAGAAAATTGGGTATGTCGATAAATACTGTTCGAA
AATATCTTAAAAGTGGTTCAGAGCCTTATTACAAAAGGCGTGATAAAAAGCCATTAAAGCTTGACCCCTATAAAGCATATATTCAAAAAAGATTAAAAGA
TGCAACACCTCATTGGGTACCAGCTACTGTGATTTATCGAGAGATTAAATTGTTTGGTTATGATGGATGCAGCTCCCAATTAAGAGCATATATGCATACT
TTAAGGTCAAAAAAAGAAGATGTTATTGTTCGGTTTGAAACAGAGCCTGGACAGCAGATGCAGGTGGATTGGGCTCAATTCCGAAGAGGAAAGGATAGAT
TAAGTGCTTTTATTGCAACCATGGGATATTCCAGAGCATCTTATGTAGAGTTTGTCAGCGATGAAACCTTGGAAACGCTTATTGCATGCCATAAAAATGC
ATTCGAATATTTTGGTGGAGTTCCTTATACGATCCTATATGACAATATGAAAACAGTCATTATTGAACGAAATGGATATGGCCTAAGTCAACATCGATTT
CAAGCTGGTTTTTGGGATTTTGCAAAACATACTGGATTTCGTCCTAAAGTGTGTCAACCTTATAGGGCACAAACAAAAGGTAAAGTTGAACGCTTTATTC
ATTATTTAAAGTACAGTTTTTATTTTCCTTTAGTTGGGCAATTGAAGGCACTTGGCTTAAGTTTAGATAAAGAGACAGCCAATATGCATGTTTTAAAATG
GCTAAATGAAATTGCTAATCAACGAGTACATGCTACAACAGGAGCAATTCCATTCGAACGACTTCTTGATGAACAAGCGAAACTACAACCATTGTCATCT
ACCTATTCTGGTAAATTATTTTCTCATTTGGAAAATAAGGAGGTTCACTATTTTGCCTTTCAATTATTAGACAACACTGCAATGCAACATGAATTGTCAA
TTTATCAGAAACTACTCGAGCGCACAGAGGAGGCTGCATGAATATTCAACATGAGCGTATTTTATCACTATGTGAACGCTTGAATTTATCAGCGATAGCG
ACAAATTACTCTTATTTAGCTCAAGAGGCAGCAACTCATAATCAAGGATTTAGTGATTTTTTAGAAAATGTATTGACTGTGGAGTTACAGGAAAAACAGT
ATCGAAGTCGAACCCTGCTCACTAAAATGGCTGGTTTTCCAATGATTAAAACAATAGATGATTTTGATTTTAAATTTGCTTCTGGTGTACCAAAAAATAA
AATCCGAGAACTGACTTCTTTGGCTTTCATTGAACGACAGGAAAATATTTTATTACTTGGTCCTAGTGGATTAGGCAAGACTCATTTAGCGATTGCTCTG
GGTTATTTAGCAGTTCAAGCAAACATTAAAACAAAATTTATTAGCGCAGCAGATTTGATGTTATTGCTTGAAACAGCTCATCGCCAAGGGCGATATAAAG
ATATTATGCGACGGGTTATTAATAATGCTCGACTGCTCATTATTGATGAAATTGGATATTTACCAATGAACCGAGATCAGGCAAATCATTTCTTCCAAGT
TATTGCAAATCGCTATGAAAAAGGTTCTATTATTGCCACCTCCAATCTCGCTTTTGGTCAATGGGACAATACTTTCGCCGGCGATAAAGTATTAACAGCG
GCTATGCTCGACAGGTTGCTCCATCATGCTCATGTCATTCAATGCAGAGGCGACAGTTATAGATTAAAAGACAAATTTAAGGCCGGTTTAATTCAAAATC
AGAAGGAGGAGCAAAATAATATAACTGATTAATTTTTTTACCAGGGGGGATCAATTTTCATCTGGCAAAAGGATCAATTTTCAATCGGCATTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1023 bp340 aa1191141+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIHILYKQGYSKKAIARKLGMSINTVRKYLKSGSEPYYKRRDKKPLKLDPYKAYIQKRLKDATPHWVPATVIYREIKLFGYDGCSSQLRAYMHTLRSKKE
DVIVRFETEPGQQMQVDWAQFRRGKDRLSAFIATMGYSRASYVEFVSDETLETLIACHKNAFEYFGGVPYTILYDNMKTVIIERNGYGLSQHRFQAGFWD
FAKHTGFRPKVCQPYRAQTKGKVERFIHYLKYSFYFPLVGQLKALGLSLDKETANMHVLKWLNEIANQRVHATTGAIPFERLLDEQAKLQPLSSTYSGKL
FSHLENKEVHYFAFQLLDNTAMQHELSIYQKLLERTEEAA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
795 bp264 aa11381932+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MNIQHERILSLCERLNLSAIATNYSYLAQEAATHNQGFSDFLENVLTVELQEKQYRSRTLLTKMAGFPMIKTIDDFDFKFASGVPKNKIRELTSLAFIER
QENILLLGPSGLGKTHLAIALGYLAVQANIKTKFISAADLMLLLETAHRQGRYKDIMRRVINNARLLIIDEIGYLPMNRDQANHFFQVIANRYEKGSIIA
TSNLAFGQWDNTFAGDKVLTAAMLDRLLHHAHVIQCRGDSYRLKDKFKAGLIQNQKEEQNNITD

 

Blast result :
Comments
In L. pneumophila str Paris, 1 IS is inserted in a gene coding a substrate of the Dot/Icm system.
2 copies are found in L. pneumophila str. Paris, 1 in L. pneumophila str. Paris plasmid pLPP, and 1 in L. pneumophila str. Corby.
ISLpn12 orfA (the Transposase) is 72% aa similar to ISGme4.
References
1] Cazalet et al., (1998) Nat Genet. Nov;36(11):1165-73.