ISCysp19
- Family IS5
- Group ISL2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011729 | ND | Cyanothece sp. | Cyanothece sp. PCC 7424 plasmid pP742401 Cyanothece sp. PCC 7424 |
DNA section
IS Length : 1214 bp
Ends
IR Length : 38/43
IRL : AGACCTCTTGCAAAAGTTTTTCGTGATATAATTAAAGGCTATTCATTTTT
IRR : AGACCTCTTGCAAAAATATTTTGTGCTATAATAAAAGGCTATTTTTCGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAGGGATAA | AAT | GTAAAAGTTG | 3 |
| TGCTCTAACC | TTT | TGCTTCTCTT | 3 |
| TTAAGTCAGT | TATCTTTAAA | 0 | |
| TAAAAGCAAT | AGACTGTTGC | 0 | |
| TACCAAAACA | AATGTAGGGA | 0 | |
| TTTTATTAAT | ATTGATGGGA | 0 |
DNA sequence
AGACCTCTTGCAAAAGTTTTTCGTGATATAATTAAAGGCTATTCATTTTTTGAGTTTGCTAGGGAGCAACTCTGATCAAACAGCGATCGCTAATCCGAAA
AAGGCAGCTAAGTTTTAAAATAAGAATAATTGTATAATCAATTAAAGATGGTTATTAAAAAAATAAAATTCATTTTATCATGAAATATTGGCAAGCTAAA
AATTTAGCATCAACAGAATTTAAGCGTTTAACAGGGGTAAAAAAAAACTTTTAGATTGATGGTTCGCACCGTCAAAAATAAAGAACAAGAAAAAAAGAAA
ATAGGGAGGAAACCAAAATTAATCGTCGAAGACCAAATTTTGATGATGCTTCAATATTTGAGAGAATATAGAACTTATTATCACATTGGTAAAGATTGGA
AAATATCCGAGTCATCTGTCTGTCGTATTGTTCATAAAATTGAAAATTTATTAATAAAATCTCGTCAATTTAGACTGCCTGGAAAGAAAGAATTATGGCA
ATCTCAGAGGCAAGAAGAACTGGTAGTAATGGATGTCATGGAAAGTCAAATTGAAAGACCTAAAAAGCGTCAAAAACAGTTTTATAGTGGAAAACCAAGA
GAACATACTTTAAAAACACAATTAGTAATTCAACAAAAAACTGGTCTAATTGTATGTTTAGTCAACGGAAAAGGAAAAACTCATGATTTTAAGCTCTTTA
AAAATAGTGGGGTTAAATTTGGGACATTAATGCAAGTTATTGCTGATAAAGGCTATCAAGGTATAGCTAAAATTCATCAATTAAGTGAAACTCCAATCAA
GAAAAAGAAAGGGAAAAAGTTAACCCAAGAAGAGAAAATATATAATCGGCAACTGAATCGATTAAGAATCACTGTCGAGCATATTAATAGACGACTAAAA
ATCTTCAAAATTCTCTCTTATCCTTATCGAAATCGTAGTAAAAGATTTGGATTAAGAGCCAACTTAATAGCTGGACTTCATAACTACGATTTGGCTAATT
AATTTGTAAAAAAAATCTATTAAATCTCTAGTTAGTTTAGCGTTTAATCATTAGCTGAACCTGTTTAGTCTGACAATTTTTAATCATTTTGGTTGGTGAT
GATCGCCGAAATCCAATTCTTCAAAAAAATTGAGAAATTTTCGCTATCTTATCAAGCCAAGTTAAACGAAAAATAGCCTTTTATTATAGCACAAAATATT
TTTGCAAGAGGTCT
AAGGCAGCTAAGTTTTAAAATAAGAATAATTGTATAATCAATTAAAGATGGTTATTAAAAAAATAAAATTCATTTTATCATGAAATATTGGCAAGCTAAA
AATTTAGCATCAACAGAATTTAAGCGTTTAACAGGGGTAAAAAAAAACTTTTAGATTGATGGTTCGCACCGTCAAAAATAAAGAACAAGAAAAAAAGAAA
ATAGGGAGGAAACCAAAATTAATCGTCGAAGACCAAATTTTGATGATGCTTCAATATTTGAGAGAATATAGAACTTATTATCACATTGGTAAAGATTGGA
AAATATCCGAGTCATCTGTCTGTCGTATTGTTCATAAAATTGAAAATTTATTAATAAAATCTCGTCAATTTAGACTGCCTGGAAAGAAAGAATTATGGCA
ATCTCAGAGGCAAGAAGAACTGGTAGTAATGGATGTCATGGAAAGTCAAATTGAAAGACCTAAAAAGCGTCAAAAACAGTTTTATAGTGGAAAACCAAGA
GAACATACTTTAAAAACACAATTAGTAATTCAACAAAAAACTGGTCTAATTGTATGTTTAGTCAACGGAAAAGGAAAAACTCATGATTTTAAGCTCTTTA
AAAATAGTGGGGTTAAATTTGGGACATTAATGCAAGTTATTGCTGATAAAGGCTATCAAGGTATAGCTAAAATTCATCAATTAAGTGAAACTCCAATCAA
GAAAAAGAAAGGGAAAAAGTTAACCCAAGAAGAGAAAATATATAATCGGCAACTGAATCGATTAAGAATCACTGTCGAGCATATTAATAGACGACTAAAA
ATCTTCAAAATTCTCTCTTATCCTTATCGAAATCGTAGTAAAAGATTTGGATTAAGAGCCAACTTAATAGCTGGACTTCATAACTACGATTTGGCTAATT
AATTTGTAAAAAAAATCTATTAAATCTCTAGTTAGTTTAGCGTTTAATCATTAGCTGAACCTGTTTAGTCTGACAATTTTTAATCATTTTGGTTGGTGAT
GATCGCCGAAATCCAATTCTTCAAAAAAATTGAGAAATTTTCGCTATCTTATCAAGCCAAGTTAAACGAAAAATAGCCTTTTATTATAGCACAAAATATT
TTTGCAAGAGGTCT
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 75 bp | 24 aa | 180 | 254 | + | No |
Description : First part of the transposase
ORF sequence :
MKYWQAKNLASTEFKRLTGVKKNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 762 bp | 253 aa | 241 | 1002 | + | No |
Description : Second part of the transposase
ORF sequence :
KKTFRLMVRTVKNKEQEKKKIGRKPKLIVEDQILMMLQYLREYRTYYHIGKDWKISESSVCRIVHKIENLLIKSRQFRLPGKKELWQSQRQEELVVMDVM
ESQIERPKKRQKQFYSGKPREHTLKTQLVIQQKTGLIVCLVNGKGKTHDFKLFKNSGVKFGTLMQVIADKGYQGIAKIHQLSETPIKKKKGKKLTQEEKI
YNRQLNRLRITVEHINRRLKIFKILSYPYRNRSKRFGLRANLIAGLHNYDLAN
ESQIERPKKRQKQFYSGKPREHTLKTQLVIQQKTGLIVCLVNGKGKTHDFKLFKNSGVKFGTLMQVIADKGYQGIAKIHQLSETPIKKKKGKKLTQEEKI
YNRQLNRLRITVEHINRRLKIFKILSYPYRNRSKRFGLRANLIAGLHNYDLAN
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 823 bp | 273 aa | 180 | 1002 | + | Yes |
Chemistry : DDE
ORF sequence :
MKYWQAKNLASTEFKRLTGVKKTFRLMVRTVKNKEQEKKKIGRKPKLIVEDQILMMLQYLREYRTYYHIGKDWKISESSVCRIVHKIENLLIKSRQFRLP
GKKELWQSQRQEELVVMDVMESQIERPKKRQKQFYSGKPREHTLKTQLVIQQKTGLIVCLVNGKGKTHDFKLFKNSGVKFGTLMQVIADKGYQGIAKIHQ
LSETPIKKKKGKKLTQEEKIYNRQLNRLRITVEHINRRLKIFKILSYPYRNRSKRFGLRANLIAGLHNYDLAN
GKKELWQSQRQEELVVMDVMESQIERPKKRQKQFYSGKPREHTLKTQLVIQQKTGLIVCLVNGKGKTHDFKLFKNSGVKFGTLMQVIADKGYQGIAKIHQ
LSETPIKKKKGKKLTQEEKIYNRQLNRLRITVEHINRRLKIFKILSYPYRNRSKRFGLRANLIAGLHNYDLAN
Blast result :
Comments
ISCysp19 is 83% aa similar to IS702.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
References
1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2008) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liberton,M., Stoeckel,J., Banerjee,A., Singh,A., Page,L., Sato,H., Zhao,L., Sherman,L., Pakrasi,H. and Richardson,P. (2008) Direct submission GenBank.