ISfinder Mirror Site

ISRhsp7

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009428	ND	Rhodobacter sphaeroides	Rhodobacter sphaeroides ATCC 17025

DNA section

IS Length : 1397 bp

Ends

IR Length : 16/22

IRL : CAGGTTGCTGAAAAACCCGGCCTCGACGCGGGTTGCAGAACGTGATTCAC
IRR : CAGGCCGCTGAAATAGTCGACCGCCCGGAATATTGTTCTTGGGCACACCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCGCCTCTCT	CTA	CATGACCCAA	3

DNA sequence

CAGGTTGCTGAAAAACCCGGCCTCGACGCGGGTTGCAGAACGTGATTCACCCTTCCGGGAAGCACGTGGGGTCTCTTGATGCGCGGGACGGACGAGACGA
GCGGGTCGCTCTTCAGCTACGTCGACCTTGAGGAGCGCATTCCGGCCCGTCACCCGCTTCGCAAGATACGCCGGGTCGTGAACGACGCGCTGGTCAGCAT
GGACGCCGAGTTCGCCCGGCTCTACGCCGCCGACGGCCGCCCCTCGATCGCACCCGAGCGTCTGCTGCGCGCCAGCCTGATCCAGATCCTGTTTTCCGTC
CGGTCCGAGCGGCAGCTGATGGAGCAGATGCAGTACAACCTGCTGTTCCGCTGGTTCGTCGGGCTGGGCATCGACGATCCGGTCTGGGTGGCCACGGTGT
TCAGCAAGAACCGCGACCGGCTGCTGACGACGGATATCGCGCAGAAGTTCCTGGCCGCGATCCTCGCCCATTGCGAGATTGCGCCGCTCCTCTCCGACGA
GCACTTCTCGGTGGATGGGACGCTCATCAAGGCCTGGGCGTCGGTGAAGAGCTTCCAGCCAAAGCCGGAGCAGGAAGCGGCAGAGCCCGGCGGCGGTCCC
GACGATCCGCCAGCCCCTCCGCCCTCTGCCGAAGCTGAACCCACCGAGACGAAGCCCGAGCCCGCCCCGATGACCCCGCCGAAAGCCAGAACCCGAAATG
CCGAGGTCGACTTCCGGGGCCAGAAGCGCTCGAACGCGACCCACGCTTCGGTCACTGATCCGGAGGCCCGGCTTTACAAGAAATCTCCCGGCGCCGGCGC
GGTCCTGTGTTTCATGGGCCATGCCCTGATGGAGAACCGGCACGGTTTCGTGGTGCAGGCCGAGGTCACGCAGGCGGACGGGCACGCCGAACGGAAGGCC
GCGCTCGACATGGTCCACCGCCATTCACCAGGGTCAACCCGACGGCTGACGCTCGGCGCCGACAAGGCCTATGACGCCGAGGAGTTCGTCCAGGACCTGC
GGCAGGCCTGCGTCACACCTCATGTCGCGCAAAAGGCGCGCTTCTCGGCGATCAACGGCCGGACCACCCGGCACGAGGGCTACGCCCTCTCGCAGCGCTG
TCGGACGAAGATCGACTTGCGTCGGGGCAACGATCCACTGGATCGTTGCCTGATCCTCCTCACCTTCGGCTGGGCCAAGACCGTGGGGCCGATGGTGCAG
ACCATGCTGCGCGGCGTGAAGCGCGTCGGCGCCCAGTTCACCTTCACGATGGCGGCCTGCAACCTCGCCAGGCTGCCGAGATTGCTTGCCGCTTGACGAC
AGGCGGCGGAATGGCGCGCGATACCTTGGGCCAGCCCCGCCTTCGACCGGTGTGCCCAAGAACAATATTCCGGGCGGTCGACTATTTCAGCGGCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1218 bp	405 aa	79	1296	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGTDETSGSLFSYVDLEERIPARHPLRKIRRVVNDALVSMDAEFARLYAADGRPSIAPERLLRASLIQILFSVRSERQLMEQMQYNLLFRWFVGLGIDD
PVWVATVFSKNRDRLLTTDIAQKFLAAILAHCEIAPLLSDEHFSVDGTLIKAWASVKSFQPKPEQEAAEPGGGPDDPPAPPPSAEAEPTETKPEPAPMTP
PKARTRNAEVDFRGQKRSNATHASVTDPEARLYKKSPGAGAVLCFMGHALMENRHGFVVQAEVTQADGHAERKAALDMVHRHSPGSTRRLTLGADKAYDA
EEFVQDLRQACVTPHVAQKARFSAINGRTTRHEGYALSQRCRTKIDLRRGNDPLDRCLILLTFGWAKTVGPMVQTMLRGVKRVGAQFTFTMAACNLARLP
RLLAA

Blast result :

Comments

ISRhsp7 is 82% aa similar to ISRosp6.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Richardson,P., Mackenzie,C., Choudhary,M., Donohue,T.J. and Kaplan,S. (2007) Direct submission GenBank.