ISPph5

  • Family ISNCY
  • Group ISDol1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011060 ND Pelodictyon phaeoclathratiforme
Pelodictyon phaeoclathratiforme BU-1
DNA section
IS Length : 1876 bp

Ends


IR Length : 12/17

IRL : GTGGATGAACGAAAAACTGTTATGATATTATAAAATAATAGTTTATATTG
IRR : GTGCCAGAAAGAAAACCCCTGTTTTTTGAAAATTTGACAACCTCCCGCCG

Insertion site


Left flankDirect repeatRight flankDR Length
ATCAGGACATCGTAAATAGTAGGACTCG6
AACGTCATCTCATAGATAGTACCGGGGA6
TAATAAAAAGTTTACGTAACACTCCCCA6
TAGGCAAACCATTTGTTAGCAATTTTTT6
ACTGCTCAACTATAATTACAATGTCGGA6
TGATGCTATCGGTAAGTATATTGCTGAT6

DNA sequence

GTGGATGAACGAAAAACTGTTATGATATTATAAAATAATAGTTTATATTGTTATTTCATGGTCAAAGATATTTGACGGACCCTGAGCTAAAGCGTAATAA
TACCCCATTAGAAGCATTTTTGATAGAGAAAAGGTGTTGCAGGGTGCCCTCAAAGAGAAACAGACTCCAAACTTCGTTTTTTGAAATCCACTATGCGTAG
CGTCATCAACCACCAAATGATGTTCGGTCGTACCGATATTTCTGCCATTGTTTTCGATCCGAAATCACGAGATGATATCCCAAAAATCTTGAGGGGCCTT
CAGGGCATCTACACCGACGAGGAGTTGCGCAAGCGTGTTTTTGCCATCCTCGAAGAGGTTCGTCCTGAACGGAAAAAAGGAGAAGGAAAAGCTGACCCAA
ACACAGGGCGTCCCGGTATGGAGCAATGGGCAATTTTGGTACTGGGTGTCGTTCGACTGGGAATCAACGCTGATTACGATCGGCTTCATGAATTGGTCAA
TCACCACGATACGCTTCGCCAGATGCTTGGTCACAACGATTGGACTGATAAAACATCTTATGAACTCCAGACCATCAAAGATAATGTCCGACTGTTTACC
CCCGAATTGCTTGATCGAATCAACCAGGAAATTGTCCGTGCAGGTCATACGTTAGTAAAAAAAAAGAGGAAGAAAATTTCCCTTTAATGGCCCGAGGTGA
TTCGTTTGTGGTGGAAACCAACGTTCACTTTCCTGCTGATACAAGCCAGTTGTTCGATGCCATTCGCAAAGCCATTGAGGTCTGTGCGAAATGGTCTTTT
GATGAAGGATTAAGTCAATGGCGCCAATACGATCATAACATCCGGCATATCAAAAGAATGCGTCGTATTGTGCAACAGCTCAAGCACTCGACATCAAAAA
AGCCGGAAAAGAAAGCTCTCAAAGATCAACAAATAAAGCAAGCACTCCTCGATTACCTTGCGGCAGTCGATTATCAGTTACAACGGGCAGTGCGTACCGG
CGCAGAACTGGGTGGCTTAAATCCGTTGCGGCTCAGCCAGCTTAACGGCTATATTGCTCATGCTGAGGTACAAATGGGGCAAATTCGTCGTCGGATAAAT
GACGAGGTTATTCCGCATGCGGAGAAAGTCTTTTCAATTTTTCAGCCACACACCGAGTGGATCAGTAAAGGCAAGGCGGGAGTGCCGGTTGAGTTGGGAT
TACGGGTCTGTATTATCGAAGATCAGTATCGTTTTATCCTGCATCATCAGGTCATGGAAAAAGTTGTCGATAGCGACATCGCAGTGTCCATTATCGAAGA
GACCAAGCAACGTTTTCCAAACTTGCGATCGATCAGCTACGACAAAGGCTTTCATAGCCCGGATAACCAACGCGACCTGAAAGCGCTTCTTGAAAAAGTT
GTTATGCCGAAAAAAGGCAAGCTCTCAAAAATCGACAAAGCTCATGAATCCGAGCCGGAATTCAGGAGACTGCGACGGCAGCATTCGGCAGTTGAATCAG
CAATCAACGCACTTGAGGTTCACGGCTTGGATATATGCCCTGATGATGGCATCAAAGGATTCAATCGCTATGTTGCCCTTGCAGTGCTGGCTCGCAACAT
TCATCGTTATGGAGCACTCTTGTACAAGCAGGATGCGAAGCGACATCGAGGGCCCTACAAAAAAGCTGCCTGAGTTCTCTCCCCAAAAAAAGAGTTTTTT
ACCAATTCGCAGGGAGAGATCCGTTCGTTATGACGCTTCCTGGAGCAAAATTCGGTAGTTTACCCATGGCGTGTCACGATCAGCACAAAAACGCCTTTTG
TTGATCATTAAAAATGGTTTTTAATCCGGCGGGAGGTTGTCAAATTTTCAAAAAACAGGGGTTTTCTTTCTGGCAC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type transcriptional
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence : No
  • Secondary structure : No-structure

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
495 bp164 aa193687+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRSVINHQMMFGRTDISAIVFDPKSRDDIPKILRGLQGIYTDEELRKRVFAILEEVRPERKKGEGKADPNTGRPGMEQWAILVLGVVRLGINADYDRLHE
LVNHHDTLRQMLGHNDWTDKTSYELQTIKDNVRLFTPELLDRINQEIVRAGHTLVKKKRKKISL

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1059 bp352 aa6151673+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SNQPGNCPCRSYVSKKKEEENFPLMARGDSFVVETNVHFPADTSQLFDAIRKAIEVCAKWSFDEGLSQWRQYDHNIRHIKRMRRIVQQLKHSTSKKPEKK
ALKDQQIKQALLDYLAAVDYQLQRAVRTGAELGGLNPLRLSQLNGYIAHAEVQMGQIRRRINDEVIPHAEKVFSIFQPHTEWISKGKAGVPVELGLRVCI
IEDQYRFILHHQVMEKVVDSDIAVSIIEETKQRFPNLRSISYDKGFHSPDNQRDLKALLEKVVMPKKGKLSKIDKAHESEPEFRRLRRQHSAVESAINAL
EVHGLDICPDDGIKGFNRYVALAVLARNIHRYGALLYKQDAKRHRGPYKKAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1481 bp493 aa1931673+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRSVINHQMMFGRTDISAIVFDPKSRDDIPKILRGLQGIYTDEELRKRVFAILEEVRPERKKGEGKADPNTGRPGMEQWAILVLGVVRLGINADYDRLHE
LVNHHDTLRQMLGHNDWTDKTSYELQTIKDNVRLFTPELLDRINQEIVRAGHTLVKKKEEENFPLMARGDSFVVETNVHFPADTSQLFDAIRKAIEVCAK
WSFDEGLSQWRQYDHNIRHIKRMRRIVQQLKHSTSKKPEKKALKDQQIKQALLDYLAAVDYQLQRAVRTGAELGGLNPLRLSQLNGYIAHAEVQMGQIRR
RINDEVIPHAEKVFSIFQPHTEWISKGKAGVPVELGLRVCIIEDQYRFILHHQVMEKVVDSDIAVSIIEETKQRFPNLRSISYDKGFHSPDNQRDLKALL
EKVVMPKKGKLSKIDKAHESEPEFRRLRRQHSAVESAINALEVHGLDICPDDGIKGFNRYVALAVLARNIHRYGALLYKQDAKRHRGPYKKAA

 

Blast result :
Comments
ISPph5 is 57% aa similar to ISCku10.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2010).
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Liu,Z., Li,T., Zhao,F., Overmann,J., Bryant,D.A. and Richardson,P. (2008) Direct submission Genbank.