ISfinder Mirror Site

ISNha8

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007964	ND	Nitrobacter hamburgensis	Nitrobacter hamburgensis X14

DNA section

IS Length : 1288 bp

Ends

IR Length : 15/16

IRL : CAGGCTGTTGAAAAAGCCAGTCGTGGCCGAGCTGTAAGCATGATTCATTG
IRR : CAGGCTGTTGAAGAAGTGTCCGGTCTGGCTTTCGGGATGGCTTGATCGCC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CAGCGGCGATGGTCGCTTAG		CTAGGTAAAGTGCTCACCTAT	0

DNA sequence

CAGGCTGTTGAAAAAGCCAGTCGTGGCCGAGCTGTAAGCATGATTCATTGGCTCCGATGCGATTTGGAGATGATGAGTGCGCGGTGGTGACAGTCTGACT
GGCGAGTTGTTCAGCTACGTTGATCTGGAGGCGCGGGTTCGACGCGATCACCCGCTTCGCGCCATCCGGGTCATGGTGAACGAAGCGCTTGTCGCTTTCG
AGCGGGAGTTCGCGGCGCTGTATTCGCCGATCGGGCGGCCGTCGATCCCACCCGAGAAGTTGCTCAGGGCGATGCTCTTGCAGGCGTTCTACTCGATCCG
CTCGGAGCGGTTGCTTATGGAGCGGCTGGAATACGACCTTCTGTTCCGCTGGTTCGTGGGCATCGGCGTCGACGATCCGGCCTGGGACCATTCGGTGTTC
TCGAAGAACCGCGACCGGCTGCTTGAGGTTGACATCGCGGCAAAGTTCCTGAGTGCGGTGCTGGCCCAGCCCAAGGTGAAGAAGCTGCTGTCGAGCGATC
ACTTCTCGGTCGACGGCACGCTGATCGAGGCCTGGGCTTCGATGAAGAGCGTCAAACCGAAGGACGGCTCTGGCGAGCCGCCGGCCGGCGGCGCCGGGCG
CAATGCCGAAGCCGACTTCCACGGCCAGACGCGGACTAACGACACGCCTGCCTCAACCACCGATCCGGACGCAAGGCTCTACAAGAAGGGCAAGGGCAAG
GAGGCGAAGCTGTGCTTCATGGGTCATGGGCTGATGGAGAACCGCCACGGCCTGTTGGTCGACGCCTGCCTGACGCTGGCCGACGGACATGCCGAGCGGG
TGGCGGCGTTGCACATGATCGAACCTCGCGCCGACCGGCCCCTGGCCATCACACTTGGCGCTGATAAGGCCTATGGCACGGAGGGCTTCGTCAACGAGCT
GCGCGCGATGAAGGTGACGCCGCACGTGGCGCAGAACACGAGCGGCCGGAGCTCGGCGATCGACGGACGCACAATGCGGCACAGCGGCTATGCCGTCAGC
CAGCGTATCCGCAAGCGCATTGAGGAAGCCTTCGGCTGGATCAAGACGGTCGCCAGCCAGGACAAGACCCGCTTCCGCGGCCGCGACCGCGTCGGATAGG
CCTTCACCTTCGCAGCCGCCGCTTACAATCTGGTGCGACTGCCGAAGCGCATGGCGGTGCCAGCATGACCCGCCGTCCCACGGCCACGCCAAACTGCTCG
ACGGCGGCTCGATCCAGATCACTTTCGCCTATCACAACGGCGATCAAGCCATCCCGAAAGCCAGACCGGACACTTCTTCAACAGCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1023 bp	340 aa	77	1099	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGGDSLTGELFSYVDLEARVRRDHPLRAIRVMVNEALVAFEREFAALYSPIGRPSIPPEKLLRAMLLQAFYSIRSERLLMERLEYDLLFRWFVGIGVDD
PAWDHSVFSKNRDRLLEVDIAAKFLSAVLAQPKVKKLLSSDHFSVDGTLIEAWASMKSVKPKDGSGEPPAGGAGRNAEADFHGQTRTNDTPASTTDPDAR
LYKKGKGKEAKLCFMGHGLMENRHGLLVDACLTLADGHAERVAALHMIEPRADRPLAITLGADKAYGTEGFVNELRAMKVTPHVAQNTSGRSSAIDGRTM
RHSGYAVSQRIRKRIEEAFGWIKTVASQDKTRFRGRDRVG

Blast result :

Comments

ISNha8 is 96% aa similar to ISNisp7.

References

1] ISfinder annotation (2010)
2] Starkenburg,S.R., Larimer,F.W., Stein,L.Y., Klotz,M.G., Chain,P.S., Sayavedra-Soto,L.A., Poret-Peterson,A.T., Gentry,M.E., Arp,D.J., Ward,B. and Bottomley,P.J. (2008) Appl. Environ. Microbiol. 74 (9), 2852-2863.