ISfinder Mirror Site

ISSpal2

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008048	ND	Sphingopyxis alaskensis	Sphingopyxis alaskensis RB2256

DNA section

IS Length : 1314 bp

Ends

IR Length : 14

IRL : CAGGCTGCGGAAAAACTCTTCCCGTTTGGGGCTCAGCGTGATTCAATTGC
IRR : CAGGCTGCGGAAAAGCGGGTGCGTTCGGCCTTTTTCTCCAACGGACCGAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCCGAAGTGGCGCTTTAG		CTAAGGCTTGTGGGAATC	0

DNA sequence

CAGGCTGCGGAAAAACTCTTCCCGTTTGGGGCTCAGCGTGATTCAATTGCGGCGTTGAGCTTGGGGGGGTGCAGCATGCGGGGGGACGACATTGGGACGG
AGAGGCTGTTTTCCTATGTGAGTTGCGAGGCTCGGGTTTCTGCGAGCCATCCGCTTCGGCCGATCCGGGCGATTGTCGATGAAGTGCTGGAGGTGCTGTC
GGCCGATTTTGAGGGGATGTACGCGAAGACGGGGCGTCCCTCGATCGCGCCTGGGAAGCTGCTGCGCGCGTTGCTGCTACAAGCCTTTTATTCGATCCGA
TCGGAACGCCAGTTGATGGAGCAGATGGACTACAATCTGCTGTTCCGCTGGTTCGTGGGTCTGTCGATGGATGCGGCGGTTTGGGACGCCTCGATGTTCA
CCAAGAACCGTGACCGGCTTCTGGAAGGTGATGTGGCGACCAGGTTCCTCGCCGCGGTCGTGGCGCAGGCCCGAGGCCGCGATCTCCTTTCAGACGAGCA
TTTCTCGGTGGACGGTACGCTGATCGACGCCTGGGCTTCGATGAACAGCTTCCGCCCCAGGGATGATGGCGAGGGACCGGCGGGGGCCGGGCGCAATGCC
GAACGCGACTTTCGCGGCGAGAAGCGGTCGAACCAGACGCATGCCTCGACCACCGATCCCGAAGCGAAGCTCTATCGCAAGGCCAACGGTCAGTCGTCGC
GCATGGCCTTCATGGGGCATGGGCTAATGGAGAACCGCAATGGCCTGGTGGTCGGCGCGCTCGTCACTCAGGCCACAGGCACCGCCGAACGTGAGGCGGC
ACTGGTTTTGGTCGATGAACTCAAAGCCACCGGCCGCATCACCCTGGGCGCGGACAAGGCTTACGACGCACGCGCGTTCGTTCAGGCTCTGCGCGCCCGC
AAGGTCACGCCGCATATCGCTCGCAACGAGCAGATCAACCAGGCCGGTGAACGACGACGCAGAAGCGCCATCGACGGTCGCACCACCCGCCATCCCGGCT
ACGCCATCAGCTTGGCGGTTCGAAAGCGGATCGAAGAAGTGTTCGGTTGGGCCAGGACCGTCGGTGGCCCGCGTAAAACGCGCCACAAGGGCACCGATCG
CGTCGGCCAGGCTTTCACCTTGACCGCCGTCGCCTGCAACCTCGTCCGGCTGCCGAAGCCAATGGTGGCCGCATGACCGTGCCCGCACTCCGCTTCAAAT
CGGATCAATCCGGGGAAATGGCCCAAAAGGCCGCCTCAAACCCCTCGCCGAAACGCGTCTAAGCCTCGGTCCGTTGGAGAAAAAGGCCGAACGCACCCGC
TTTTCCGCAGCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1101 bp	366 aa	76	1176	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGDDIGTERLFSYVSCEARVSASHPLRPIRAIVDEVLEVLSADFEGMYAKTGRPSIAPGKLLRALLLQAFYSIRSERQLMEQMDYNLLFRWFVGLSMDA
AVWDASMFTKNRDRLLEGDVATRFLAAVVAQARGRDLLSDEHFSVDGTLIDAWASMNSFRPRDDGEGPAGAGRNAERDFRGEKRSNQTHASTTDPEAKLY
RKANGQSSRMAFMGHGLMENRNGLVVGALVTQATGTAEREAALVLVDELKATGRITLGADKAYDARAFVQALRARKVTPHIARNEQINQAGERRRRSAID
GRTTRHPGYAISLAVRKRIEEVFGWARTVGGPRKTRHKGTDRVGQAFTLTAVACNLVRLPKPMVAA

Blast result :

Comments

ISSpal2 is 73% aa similar to ISMex28.

References

1] ISfinder annotation (2010)
2] Lauro,F.M., McDougald,D., Thomas,T., Williams,T.J., Egan,S., Rice,S., DeMaere,M.Z., Ting,L., Ertan,H., Johnson,J., Ferriera,S., Lapidus,A., Anderson,I., Kyrpides,N., Munk,A.C., Detter,C., Han,C.S., Brown,M.V., Robb,F.T., Kjelleberg,S. and Cavicchioli,R. (2009) Proc. Natl. Acad. Sci. U.S.A. 106 (37), 15527-15533.