ISfinder Mirror Site

ISHha2

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008789	ND	Halorhodospira halophila	Halorhodospira halophila SL1

DNA section

IS Length : 1339 bp

Ends

IR Length : 14/16

IRL : CAGGCTGCTGAAAAACTCGGCTGCGCGGTATCATAGAGGGTGTCGTTGAC
IRR : CAGGGTGCTGAGAAACGGTCTTCTGCGTGGCCACCGAGGGGTGTCGAAGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCCGCCTCCT	CTAG	TCAAAGGAGG	4

DNA sequence

CAGGCTGCTGAAAAACTCGGCTGCGCGGTATCATAGAGGGTGTCGTTGACGGCGGAACAGGAGGTCTCCGGATGCGCGGTGAGGATGTCGAGCAACAGCA
CATGTTCAGCTACGTCAGCCTCGAAGATCGCATCCCCAAGAATCACCCTCTTCGCGAGATCCGTGTCCTCGTTGATCGGCTGCTCGACTCGATCTCGGAC
GAGCTGGACGCGGTGTACTCGGGCACCGGTCGGCCGTCGATCCCGCCGGAGCGACTGATCCGGGCGCTTTTGCTTCAGGCCCTGTACTCGATCCGCTCGG
GGCGGCTGCTCATGGAGCAGCTCGACTGCAACCTCCTGTTCCGCTGGTTCGTCGGGCTCCAGGTCGATGACCCCGTCTGGCACCCGACCACCTTCACCAA
GAGCCGGGACCGCCTGATCGAGGCGGAGGTTGCGCAGCGCCTGCTGCGCTCGTTCACGGAACTCGAGGAGGTTCGGCCCCTGCTCTCCGACGAGCACTTC
TCGGTCGATGGCACGCTCATCGAGGCCTGGGCCTCGATGAAGAGCTTCAAGCCGATCAGCCAGGACGGCGACAGCTCGGGTGATGATGACTTGCAGAGCG
GCGGGCGCAATCCGACGGTCAACTTCCGGGGTCAGCAGCGGCGCAACGATACCCACGCCTCGAGCACGGACCCGAATGCTCGGCTCTACCGCAAGGGCCA
GGGGCAGCCTGCGCGGCTTTGCTATATCGGCCATGCCCTGATGGAGAACCGTCATGGGCTCATCGTCGATACCCGCCTAACCCAGGCCTGTGGCACCGCC
GAGCGGGAGGCGGCCCTGGAGATGATCCGCGAGATCCCTGCAGAGCGAGGCCGGCTGACGCTTGCTGGCGACGCCGGCTACAACACCCGAGATTTCGTCC
AGGCCCTGCGCGAGTACGAGGTTACGCCCCATGTTGCCGAGAAGCGCCGGTTCAATGCTGTGGATGGTCGGACGACGCGCCACCCCGGCTATGCGGTCAG
CCAGCGCATCCGCAAGCGCGTCGAGGAGTTCTTCGGCTGGTCCAAGACCGTCGGTGGCCTTCGCAAGACCCGCTTCATCGGACCAGACAGGGTGGGGTGG
GACTTCGGGTTCCATGCCCTGGCCTACAACCTGGTCCGCACACCCAAACTGCTCGGGGCTGGCTGAGATGGGGGCGGAGTGCCTCCATCCCCTGCTGCCC
GGCCCCTTCCGGGCGCAGGCGGGTTGGGGCACCGCCGGGTTGGCGGGTATCACCCCCCGCTTGGAACGCCACCCGGCCATCGTAGATCACCTTCGACACC
CCTCGGTGGCCACGCAGAAGACCGTTTCTCAGCACCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1095 bp	364 aa	72	1166	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGEDVEQQHMFSYVSLEDRIPKNHPLREIRVLVDRLLDSISDELDAVYSGTGRPSIPPERLIRALLLQALYSIRSGRLLMEQLDCNLLFRWFVGLQVDD
PVWHPTTFTKSRDRLIEAEVAQRLLRSFTELEEVRPLLSDEHFSVDGTLIEAWASMKSFKPISQDGDSSGDDDLQSGGRNPTVNFRGQQRRNDTHASSTD
PNARLYRKGQGQPARLCYIGHALMENRHGLIVDTRLTQACGTAEREAALEMIREIPAERGRLTLAGDAGYNTRDFVQALREYEVTPHVAEKRRFNAVDGR
TTRHPGYAVSQRIRKRVEEFFGWSKTVGGLRKTRFIGPDRVGWDFGFHALAYNLVRTPKLLGAG

Blast result :

Comments

ISHha2 is 71% aa similar to ISNisp7.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Saunders,E., Brettin,T., Bruce,D., Han,C., Tapia,R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Hoff,W. and Richardson,P. (2007) Direct submission GenBank.