ISfinder Mirror Site

ISPst12

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009434	ND	Pseudomonas stutzeri	Pseudomonas stutzeri A1501 Pseudomonas putida plasmid pW2 Pseudomonas aeruginosa clone Def1

DNA section

IS Length : 1331 bp

Ends

IR Length : 15

IRL : CAGGCCGTTGAAAAAAGCCAGAGCCCGCGTGGCTCTGGCAAAATGGCGGC
IRR : CAGGCCGTTGAAAAACTCGGCTTCAACTGCCCCAACCCGTCCGGTTTGGC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GCTCTTGCGC	CTAG	GTTGTCCGGG	4
CGGGAACGCC	GTAG	GAGGCCATGT	4
NNNGAGCCNT	CTAG	GGCTGACATG	4

DNA sequence

CAGGCCGTTGAAAAAAGCCAGAGCCCGCGTGGCTCTGGCAAAATGGCGGCCATCCTCTTCTGCCGAAGCCAGCCCAAGCCGATGCGTGGACTCGACCTCA
AACAAAACGAGCTGTTCAGTTATACGACGCTGGAGCAGCGCATCCCGAACGATCACCCGCTGCGTCCCCTGCGAGGCCTGGTCGATACCGTTCTGGCTTC
GATGGATCGGGACTTCGACGGGCTGTACTCCACCCTGGGACGGGCCTCGATTGCGCCGGAGCGGCTGCTGCGCGCCTCGTTGCTGCAGGTGATTTACACC
ATTCGCTCCGAGCGGCAGTTGGTCGAGCAGATTGATTTCAACCTGCTGTTTCGCTGGTTCGTCGGCTTGTCGATGGACGAGCGCATGTGGGATCACTCGA
CCTTCAGCCAGAACCGTGACCGCTTGTTCAACCAGGATGTAGCGCGGCTGTTCTTCCAGCGCATCAAGTCGCTGGCCGCATGGTCCGAGTACGCCAGCAA
CGAGCATTTCAGCGTCGATGGCACGCTGATCGACGCCTGGGCCTCGCACAAGTCCTTTATCAAGAAGGATGGCGGCGACCCACCGGAGGATGGCACGCGC
AACCCCGATGCCGACTTCAAGGGCGAGAAGCGCAGCAACGCGACCCACCAATCGACCAGCGATCCCGAGGCCCGGCTGGCGCGCAAGAGCAATGGCGATG
CCAGTCGTCTGGCGCACATGGCCCACACGATGATGGAGCACCGCAACGGTCTGATCGTGGATGTGGAATGCACCGAGTTCAATGGACGTGCCGAGGTAGA
GGCGGCGCTGGAGATGCTGGAGCGCACGGCCAAGCCGGGCAGCACGGTAGGTGCAGACAAGAATTACGACCAGAAGCGTTTCGTGCAGAGGGCGCGCGAG
CTGAAAGTGACACCGCATGTGGCGCAGAAGCGCAAGGGCAGCGCCATCGATGGGCGCACCACGCGGCATCCGGGGTATGCCGCCAGCCAGAAGATCCGCA
AGCGGATCGAAGAAGGTTTTGGCTGGCTGAAGACGGTTGGCGGCCTGCGCAAGACCAAGCTGATCGGTCGCGCCAAGCTGAGTGCTCAGTTATTGCTGGG
TTTCTCGGTCTACAACCTGATCCGACTGGGTAGCCTGTCGGGTTGGTGGCGAGGATCGCATGTATAGGGCGAGTTACGCCCAAAAAACGCCGAAAGGCGT
GAACGTGGTGCTGAAACCTGTCAAAAAGGCCTGAAGTCAGGCCTTGTGTGGACTCCGTTAGGCCAAAGCGCTTGAAAAAGCGCCAAACCGGACGGGTTGG
GGCAGTTGAAGCCGAGTTTTTCAACGGCCTG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1086 bp	361 aa	82	1167	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGLDLKQNELFSYTTLEQRIPNDHPLRPLRGLVDTVLASMDRDFDGLYSTLGRASIAPERLLRASLLQVIYTIRSERQLVEQIDFNLLFRWFVGLSMDE
RMWDHSTFSQNRDRLFNQDVARLFFQRIKSLAAWSEYASNEHFSVDGTLIDAWASHKSFIKKDGGDPPEDGTRNPDADFKGEKRSNATHQSTSDPEARLA
RKSNGDASRLAHMAHTMMEHRNGLIVDVECTEFNGRAEVEAALEMLERTAKPGSTVGADKNYDQKRFVQRARELKVTPHVAQKRKGSAIDGRTTRHPGYA
ASQKIRKRIEEGFGWLKTVGGLRKTKLIGRAKLSAQLLLGFSVYNLIRLGSLSGWWRGSHV

Blast result :

Comments

ISPst12 is 77% aa similar to ISDisp1.

References

1] ISfinder annotation (2010)
2] Yan,Y., Yang,J., Dou,Y., Chen,M., Ping,S., Peng,J., Lu,W., Zhang,W., Yao,Z., Li,H., Liu,W., He,S., Geng,L., Zhang,X., Yang,F., Yu,H., Zhan,Y., Li,D., Lin,Z., Wang,Y., Elmerich,C., Lin,M. and Jin,Q. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (21), 7564-7569.