ISMlo5
- Family ISNCY
- Group ISDol1
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AL672114 | ND | Mesorhizobium loti | Mesorhizobium loti |
DNA section
IS Length : 1664 bp
Ends
IR Length : 18/19
IRL : GGCGCGTCCATAAACGGCAAGCTGCTGATATTATTGAATGAATCCTGATT
IRR : GTGCGCGTCCATAAACGGCTGTTTTTCCGGAGTTTTCAGTGGTCTTTGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCACCTAGTC | TAGACTA | GACTAGAGGC | 7 |
DNA sequence
GGCGCGTCCATAAACGGCAAGCTGCTGATATTATTGAATGAATCCTGATTTTGGGTAGGCAAGGCCCGGCGGGTTCAGATACACTTCGAGATCAAGCCAC
TGATTCCAAAGCGTTTCTGCCACCGCCGGAGCCGACAATGCGCCAAGAACGCACCGTCCAATCCAGTGTATTTGATCTTTTCGCCGGACACGAGATCGGC
TGTGAATTGAAAGCCATGTCGCAATGGCTGGACGGGCATCGCGAGCTTTTGAGCCTGGTGACGCGGGACCTGCGCCGGCACGATCTCAAGGAGACCGGCC
GCGAGGGCCTGCCGGCGGAGGCTGTGCTGCGCTGCGCGCTGCTCAAGCAGCACCGCCAACTAAGCTATCAGGAACTGGCCTTTCACTTGGAGGATTCCGC
CTCGTTCCGGGCTTTTGCCCGGCTGCCATGGGGGTGGAGCCCAAAGAAATCAGTCCTGCACAAGACGATCAGCGCGATCCAAGCGCCGACCTGGGAAGAA
ATCAACAAAGTACTGCTGGCAAGCGCCGGGCGAGAGAAGCTGGAAGGCGGCAAGGTCGTGCGCGTGGACAGCACCGTCACCGCCGCACTCGTCCACGAGC
CGAGCGACAGCAGCCTGTTATGGGACGCTGTGCGGGTGATGGTGCGGCTGTTGCAGCACGCGGATGCACTGGGCAGCGCCATCCCATGGCACGATCACTG
CCGCGCGGCGAAGAAGCGGTCCCGGTCGATCGAATATACCCGCGGCCGCCCGAAACGGGTGAAGCACTACCGCGAGCTGCTCAGGATCACACGCACCACC
TTGGGCTATCTCCAGCAGGCGGCTGAGCAGCTGACCCTGGCGGCGTGCCCGGCCGTTGAACTGTGGCAGGCCCAAGTCCGCCATTACCGGCCGCTGATCG
AGCGGATCATCGCCCAGACCGAACGGCGGGTCCTGGCAGGCGAACCGGTCCCGGCCGGCGAGAAGCTGGTGAGCCTGTTCGAGCCGCATGCCGATATCAT
CGTCAAGGGCAGCCGCGACGTCGATTATGGTCACAAGATCAATCTGACGACCGGTCGCAGCGGACTGATCCTCGATCTCGTCATCGAAGCGGGCAACCCA
GCCGACAGCGAGCGTCTGCTGCTAATGCTGGAGCGCCACATCGCGTTTTACGGCGAGCCGCCGCGTCAGGCGGCGGCCGACGGCGGCTATGCCAGCCGCC
CCAATCTGCGCCAGGCCAAGGCTTGGGGCGTGCGCGATATGGCGTTCCACAAGAAGAGCGCCCTCAGGATCGAAGACATGGTCAGCAGCCGCTGGGTCTA
TCGCAAGCTGCGCAACTTCCGCGCTGGTATCGAGGCCAGCATCTCCTGCCTGAAGCGCGCCTATGGCTTGGCCCGCTGCACCTGGCGCGGGCTCAACCAC
TTCAAGGCTTACGTCTGGTCCTCGGTGGTCGCTTACAATCTCGCCCTCTTCACCCGCCTCAGATCGAACTGACCCCAGTCATCGGGCTGCGTTCAGGAGC
ACGCCGGCGAGCGCCGGCCGATACCCCTATGACCCCATTGCCGCCAGATCGACCCCCGGCCGCAACCTCCATGCCCTTGTACCATCACGCCTCCCCAAAA
CATCGCTTCAGCAGGCCAAAGACCACTGAAAACTCCGGAAAAACAGCCGTTTATGGACGCGCAC
TGATTCCAAAGCGTTTCTGCCACCGCCGGAGCCGACAATGCGCCAAGAACGCACCGTCCAATCCAGTGTATTTGATCTTTTCGCCGGACACGAGATCGGC
TGTGAATTGAAAGCCATGTCGCAATGGCTGGACGGGCATCGCGAGCTTTTGAGCCTGGTGACGCGGGACCTGCGCCGGCACGATCTCAAGGAGACCGGCC
GCGAGGGCCTGCCGGCGGAGGCTGTGCTGCGCTGCGCGCTGCTCAAGCAGCACCGCCAACTAAGCTATCAGGAACTGGCCTTTCACTTGGAGGATTCCGC
CTCGTTCCGGGCTTTTGCCCGGCTGCCATGGGGGTGGAGCCCAAAGAAATCAGTCCTGCACAAGACGATCAGCGCGATCCAAGCGCCGACCTGGGAAGAA
ATCAACAAAGTACTGCTGGCAAGCGCCGGGCGAGAGAAGCTGGAAGGCGGCAAGGTCGTGCGCGTGGACAGCACCGTCACCGCCGCACTCGTCCACGAGC
CGAGCGACAGCAGCCTGTTATGGGACGCTGTGCGGGTGATGGTGCGGCTGTTGCAGCACGCGGATGCACTGGGCAGCGCCATCCCATGGCACGATCACTG
CCGCGCGGCGAAGAAGCGGTCCCGGTCGATCGAATATACCCGCGGCCGCCCGAAACGGGTGAAGCACTACCGCGAGCTGCTCAGGATCACACGCACCACC
TTGGGCTATCTCCAGCAGGCGGCTGAGCAGCTGACCCTGGCGGCGTGCCCGGCCGTTGAACTGTGGCAGGCCCAAGTCCGCCATTACCGGCCGCTGATCG
AGCGGATCATCGCCCAGACCGAACGGCGGGTCCTGGCAGGCGAACCGGTCCCGGCCGGCGAGAAGCTGGTGAGCCTGTTCGAGCCGCATGCCGATATCAT
CGTCAAGGGCAGCCGCGACGTCGATTATGGTCACAAGATCAATCTGACGACCGGTCGCAGCGGACTGATCCTCGATCTCGTCATCGAAGCGGGCAACCCA
GCCGACAGCGAGCGTCTGCTGCTAATGCTGGAGCGCCACATCGCGTTTTACGGCGAGCCGCCGCGTCAGGCGGCGGCCGACGGCGGCTATGCCAGCCGCC
CCAATCTGCGCCAGGCCAAGGCTTGGGGCGTGCGCGATATGGCGTTCCACAAGAAGAGCGCCCTCAGGATCGAAGACATGGTCAGCAGCCGCTGGGTCTA
TCGCAAGCTGCGCAACTTCCGCGCTGGTATCGAGGCCAGCATCTCCTGCCTGAAGCGCGCCTATGGCTTGGCCCGCTGCACCTGGCGCGGGCTCAACCAC
TTCAAGGCTTACGTCTGGTCCTCGGTGGTCGCTTACAATCTCGCCCTCTTCACCCGCCTCAGATCGAACTGACCCCAGTCATCGGGCTGCGTTCAGGAGC
ACGCCGGCGAGCGCCGGCCGATACCCCTATGACCCCATTGCCGCCAGATCGACCCCCGGCCGCAACCTCCATGCCCTTGTACCATCACGCCTCCCCAAAA
CATCGCTTCAGCAGGCCAAAGACCACTGAAAACTCCGGAAAAACAGCCGTTTATGGACGCGCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1335 bp | 444 aa | 138 | 1472 | + | No |
Chemistry : DDE
ORF sequence :
MRQERTVQSSVFDLFAGHEIGCELKAMSQWLDGHRELLSLVTRDLRRHDLKETGREGLPAEAVLRCALLKQHRQLSYQELAFHLEDSASFRAFARLPWGW
SPKKSVLHKTISAIQAPTWEEINKVLLASAGREKLEGGKVVRVDSTVTAALVHEPSDSSLLWDAVRVMVRLLQHADALGSAIPWHDHCRAAKKRSRSIEY
TRGRPKRVKHYRELLRITRTTLGYLQQAAEQLTLAACPAVELWQAQVRHYRPLIERIIAQTERRVLAGEPVPAGEKLVSLFEPHADIIVKGSRDVDYGHK
INLTTGRSGLILDLVIEAGNPADSERLLLMLERHIAFYGEPPRQAAADGGYASRPNLRQAKAWGVRDMAFHKKSALRIEDMVSSRWVYRKLRNFRAGIEA
SISCLKRAYGLARCTWRGLNHFKAYVWSSVVAYNLALFTRLRSN
SPKKSVLHKTISAIQAPTWEEINKVLLASAGREKLEGGKVVRVDSTVTAALVHEPSDSSLLWDAVRVMVRLLQHADALGSAIPWHDHCRAAKKRSRSIEY
TRGRPKRVKHYRELLRITRTTLGYLQQAAEQLTLAACPAVELWQAQVRHYRPLIERIIAQTERRVLAGEPVPAGEKLVSLFEPHADIIVKGSRDVDYGHK
INLTTGRSGLILDLVIEAGNPADSERLLLMLERHIAFYGEPPRQAAADGGYASRPNLRQAKAWGVRDMAFHKKSALRIEDMVSSRWVYRKLRNFRAGIEA
SISCLKRAYGLARCTWRGLNHFKAYVWSSVVAYNLALFTRLRSN
Blast result :
Comments
ISMlo5 is 92% aa similar to ISRm17 and ISRle10.
References
1] ISfinder annotation (2010).
2] Sullivan,J.T., Trzebiatowski,J.R., Cruickshank,R.W., Gouzy,J., Brown,S.D., Elliot,R.M., Fleetwood,D.J., McCallum,N.G., Rossbach,U., Stuart,G.S., Weaver,J.E., Webby,R.J., De Bruijn,F.J. and Ronson,C.W. (2002) J. Bacteriol. 184 (11), 3086-3095.
3] Ronson,C.W. (2002) Direct submission GenBank.
2] Sullivan,J.T., Trzebiatowski,J.R., Cruickshank,R.W., Gouzy,J., Brown,S.D., Elliot,R.M., Fleetwood,D.J., McCallum,N.G., Rossbach,U., Stuart,G.S., Weaver,J.E., Webby,R.J., De Bruijn,F.J. and Ronson,C.W. (2002) J. Bacteriol. 184 (11), 3086-3095.
3] Ronson,C.W. (2002) Direct submission GenBank.