ISfinder Mirror Site

ISDce1

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_013216	ND	Desulfotomaculum acetoxidans	Desulfotomaculum acetoxidans DSM 771

DNA section

IS Length : 1904 bp

Ends

IR Length : 15/16

IRL : GAGACTGTTTCAAAAATCAATCTCCCTAATATAATGTATAGATATTGCAA
IRR : GAGACCGTTTCAAAAATACTCTTTTTTTGATTTACCTTAATAATTTCAAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTTGCCTCAC	CTAG	GAGAGTGTTT	4
GGATGTCATT	CTAG	GTGTGCTGCT	4

DNA sequence

GAGACTGTTTCAAAAATCAATCTCCCTAATATAATGTATAGATATTGCAAAGCAGGCTTTAACCTAATTAATAAGATCCAAAGAAAGTCCCCAAAAATAC
GAGTAAATATGGTATAATTAAAATAAATAACCATATGCGGGGGTATTTAGCCATGATGGGTAAACTAACAAATCAAATTAACTTTTCTGATACAGATGAA
TGGTATAAACGAATACCCCAAAATTCATTTTGGCATAAAGTGCGTCAATGGGCAGAAGAAAATCTGTCAGATGATGACTTTGCCCATCTATATTCCAGTA
ATAGGGGACGCCCGTCATTACCCCCGGTGTTTATGCTGAAATCCATACTGATACAGCTTGAAAAACAATACTCCGATCGTGTTATGGAAGAATCAGCAAT
GTTCGATGACCGCGTAAAATATGCACTTTGCCTTAGCCGTACCCCCCAGATAAAACTTGACCATGCCACATTATGCAGATATCGCAAAATATTCTTAGAA
GACGAGCAGGGAAAGAAAATATTAAAGAAAACAATAGAAAATGCAGCAGAAGCAGGGCTATTCGAAGAAGCTAATAAAGATGCTGTCGACTCATTTATGA
TTCATGGAGCGGCAGCCCGTCAAAGTACCTTTACCATGATCCGGAAAGCCACAGCCCGGGTATTACGCCAGGCTGACGTTGAGGGCTTTATTGAAGATAT
AAGACAAAAACTCAATCGTGATGATTACCTTAACAATAAAAAACCCACTATAGACTGGGATAACATAGAAGCTCGCAATAAATTACTCACAGAAATGGTC
CTTGACGCCAGAACAATAGCCTTATGGGCAAAAGAGAACAAAGCCAAAATCAGTGAAGAACTAAACCAATGCATAGAACTTTTGCAAATAGTAGCAGAGC
AGGACATTGAAGAAAAAGATGGAAATATAGCCATCCGTCAGGGTGTAGCTAAAGACAGAATCATATCAGTAGAAGATCCGCAAATGCGTCATGGACGCAA
AACAACGAGCAGTAAAACAGATGGTTACAAGGGACACATCATGTCCGGCGGTATAGAAAACAAGATAATAACAGCTGCAGAAATAACTGCTGCTAACGTT
CCGGACAGTGAACCTGTACCTGACTTAATCAAGCAGCGTCAAGAAAATACAGGAAGTAAACCTGATTCCCTTAGCGGTGATACCGCATATGGTGGTGCTG
AAACCAGAAAACACATTAAAAAAGAAAAAATCAAACTAATCGCCAAAGTACCTCCGTCAACCAATGTAAATGGATGTTTTAATAAAGACAAATTCATCAT
TGATCTGGATAACAAATTTATAGAATGCCCTGCCGGAGTTCGACTTGAAATAGATAAGGAACTGGGAGAAAAGGAAATATGCTGTAAATTCCCAAAAGAA
CAGTGTCAAAATTGCGATCTAAGAAATCAATGCACCAAAAGTAAAGATGGCAGAACAGTAAGAATACATCCGCATGAAGCATTATTGCAAAAGGCACGTA
AACAACAACAAACAGCAGAGTTTAAAGAGGAGTATCGTTTCCGTAGTCGGATTGAGAGAATAATTTATTGTGTTACTAAAAATGGAGCCCGAAAGGGTAA
ATATAATGGACTTGAAAAAAATAGATTTAAGTTGCAACTCCATACGGCTTTACATAATATCAAAACAATACTTTCTTTAGCTAAAAAAAATCCTGCGATA
GTTGTATAGGGATAAGTGTGTCTATTCGTTAGGAATTATATGGAAAATGGTGTTGTGTATGCTTCATTATGCTGTTTAATTAAATTGTTTGATGCTCTTT
TGATAGAAACAATCACCAAATACTGTGGTTTTATCTATTAATTCCAGTTGTGTAGTTGAAATTATTAAGGTAAATCAAAAAAAGAGTATTTTTGAAACGG
TCTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1557 bp	518 aa	153	1709	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MMGKLTNQINFSDTDEWYKRIPQNSFWHKVRQWAEENLSDDDFAHLYSSNRGRPSLPPVFMLKSILIQLEKQYSDRVMEESAMFDDRVKYALCLSRTPQI
KLDHATLCRYRKIFLEDEQGKKILKKTIENAAEAGLFEEANKDAVDSFMIHGAAARQSTFTMIRKATARVLRQADVEGFIEDIRQKLNRDDYLNNKKPTI
DWDNIEARNKLLTEMVLDARTIALWAKENKAKISEELNQCIELLQIVAEQDIEEKDGNIAIRQGVAKDRIISVEDPQMRHGRKTTSSKTDGYKGHIMSGG
IENKIITAAEITAANVPDSEPVPDLIKQRQENTGSKPDSLSGDTAYGGAETRKHIKKEKIKLIAKVPPSTNVNGCFNKDKFIIDLDNKFIECPAGVRLEI
DKELGEKEICCKFPKEQCQNCDLRNQCTKSKDGRTVRIHPHEALLQKARKQQQTAEFKEEYRFRSRIERIIYCVTKNGARKGKYNGLEKNRFKLQLHTAL
HNIKTILSLAKKNPAIVV

Blast result :

Comments

ISDce1 is 41% aa similar to ISAcma45.

References

1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N., Mavromatis,K., Ivanova,N., Mikhailova,N., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Spring,S., Klenk,H.-P. and Eisen,J.A. (2009) Direct submission GenBank.