ISLca3
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008526 | ND | Lactobacillus casei | Lactobacillus casei ATCC 334 |
DNA section
IS Length : 1565 bp
Ends
IR Length : 13/15
IRL : GTGAGTGATTAATAATTCTTAACACTATGAATCAATACCCTTCGTCCCAC
IRR : GTGAGTGATGAATTAGCCCCTACCATCTAGCTCTAAATACTATCGTTGTA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTCAGAAGCA | TAGATA | AATGTATCAA | 6 |
| ATTTCTGCGC | TATACTA | GGAATTACAA | 7 |
| TTTATTAAGCCACTAATTA | TAAATAGAGGATAAA | 0 | |
| ATTGAAAGGT | TACTTA | TGCAGCTGCT | 6 |
| GGCGGTACCT | TAACTTA | TCAGATAAAA | 7 |
| TGCGGCCTCC | TAAATTA | GTGTGGTTTT | 7 |
| TGGATTTTTC | TAAATA | AGTGATACCT | 6 |
DNA sequence
GTGAGTGATTAATAATTCTTAACACTATGAATCAATACCCTTCGTCCCACTTTTGTTGTAAAATAAATGTATATAATTTCCCGTTATTTCGGTAAAACCC
GTGCATTTTTCACGAAAGGTATTCATTCCATGTATGCATCTAAGGTTGTTCAGCTATCAATTGAAGCGTTTGGTGAAGCAAATGGCGTAAAACTTAGCCC
TGATAATCGTTGGGTTCAAGCAGCTACCTTGATTCCGTGGCCCGAGCTTGAAGAAGCCTACAAAACTGTCTTTCCTTCAAACAAAGGCCGTGCAGGCAAA
CCATTTCGCTTGTTATTTGGCGCGCATCTAATCAAAACTCGTGAAGGTTTGTCAGATCGAGAATTAGTTGATGCCATTCGTGATGTACCAGCGTACCAAT
TCTTCATGGGACTACCTGAGTATCAACCACGGCGGCCATTTGTTCCGTCCGCAATGGCTCATTTCCGCAAGCGTATTGCCCCAATCGCTGAGCTTCTGGA
GAACATCATTGGTGACTGGACAAGAAAGCAACTAGTTGGGTCGGTCGATGACAAAGTTGTGATCCTTGACGCAACGGTAACGCCAGTAAACATCAAGTAT
CCTCAAGATTACGTTCTCCTAGGCCAAGCTCGGCGGAACACTGAAGCGATGGTTACGGATTTGGCACATAAGCTAGAAGTGCCTATTCCGCGAACATATC
GGCGTGAGGCAGAGCGTAATTATGTCCACTTTACTAAACATCCCAAACGAACTCGTAAGGCAACGCGCGGGATGGTGAAGTCACAGCTTCAATATCTACG
CCGAAATCTGCGTTACATTCATGAATTTCAAGACCAAGGTGGTCAACTGACATCTAAACAAACACAGCGACTGGAAGTTATTGAAACGTTGTACGAACAA
CAGGACTACATGTACCGGAACAATACGCATAGTGTTGAACGCCGCATAATAAGTTTGAGTCAACCATTTGTGCGCCCAATAAAGCGAGGTAAGGCAAAAC
CTAGGAACGGAACCGAGTTCGGCCCCAAGGTTGATGTCTCGATTATTGATGGCGTGGTCGGTATTGAACGGTTTTCGTTTGATAACTTCAACGAAAATCA
GGACTTTGAGGACGCGTTGTACCACTACCATGATGTACACGGTGAGTTTCCAGACATGGCACTGGTGGATAAACTATACCGAACACGTGAGAACCTACGC
ATCGCCAAGAATTTAGGTGTAAAGGTGTGCGGTCCTAAGCTCGGTCGCCAGCCCAAATATCCAGATGAAGCCAGTGAGCGAAAGGCGAAGAAAGAGGCAC
GTGAAGCGGAGAATGCCCGTGGTGAGATTGAACGCAACTTCGCGCTAATGAAATTGAAGCACGGGTTAGGTTTGGTGCGCGCAAAAACCGTTGAAACCAT
CACTACAACGGTGTTTACCGCGATGATCACTGCAAATATTGACGCAGTACTCCGGCTTTTTTCTTTGCCGTTTGTGCTATATGTGCGATATGAAGGACAA
ATACTCAAGATTGAGTACAACGATAGTATTTAGAGCTAGATGGTAGGGGCTAATTCATCACTCAC
GTGCATTTTTCACGAAAGGTATTCATTCCATGTATGCATCTAAGGTTGTTCAGCTATCAATTGAAGCGTTTGGTGAAGCAAATGGCGTAAAACTTAGCCC
TGATAATCGTTGGGTTCAAGCAGCTACCTTGATTCCGTGGCCCGAGCTTGAAGAAGCCTACAAAACTGTCTTTCCTTCAAACAAAGGCCGTGCAGGCAAA
CCATTTCGCTTGTTATTTGGCGCGCATCTAATCAAAACTCGTGAAGGTTTGTCAGATCGAGAATTAGTTGATGCCATTCGTGATGTACCAGCGTACCAAT
TCTTCATGGGACTACCTGAGTATCAACCACGGCGGCCATTTGTTCCGTCCGCAATGGCTCATTTCCGCAAGCGTATTGCCCCAATCGCTGAGCTTCTGGA
GAACATCATTGGTGACTGGACAAGAAAGCAACTAGTTGGGTCGGTCGATGACAAAGTTGTGATCCTTGACGCAACGGTAACGCCAGTAAACATCAAGTAT
CCTCAAGATTACGTTCTCCTAGGCCAAGCTCGGCGGAACACTGAAGCGATGGTTACGGATTTGGCACATAAGCTAGAAGTGCCTATTCCGCGAACATATC
GGCGTGAGGCAGAGCGTAATTATGTCCACTTTACTAAACATCCCAAACGAACTCGTAAGGCAACGCGCGGGATGGTGAAGTCACAGCTTCAATATCTACG
CCGAAATCTGCGTTACATTCATGAATTTCAAGACCAAGGTGGTCAACTGACATCTAAACAAACACAGCGACTGGAAGTTATTGAAACGTTGTACGAACAA
CAGGACTACATGTACCGGAACAATACGCATAGTGTTGAACGCCGCATAATAAGTTTGAGTCAACCATTTGTGCGCCCAATAAAGCGAGGTAAGGCAAAAC
CTAGGAACGGAACCGAGTTCGGCCCCAAGGTTGATGTCTCGATTATTGATGGCGTGGTCGGTATTGAACGGTTTTCGTTTGATAACTTCAACGAAAATCA
GGACTTTGAGGACGCGTTGTACCACTACCATGATGTACACGGTGAGTTTCCAGACATGGCACTGGTGGATAAACTATACCGAACACGTGAGAACCTACGC
ATCGCCAAGAATTTAGGTGTAAAGGTGTGCGGTCCTAAGCTCGGTCGCCAGCCCAAATATCCAGATGAAGCCAGTGAGCGAAAGGCGAAGAAAGAGGCAC
GTGAAGCGGAGAATGCCCGTGGTGAGATTGAACGCAACTTCGCGCTAATGAAATTGAAGCACGGGTTAGGTTTGGTGCGCGCAAAAACCGTTGAAACCAT
CACTACAACGGTGTTTACCGCGATGATCACTGCAAATATTGACGCAGTACTCCGGCTTTTTTCTTTGCCGTTTGTGCTATATGTGCGATATGAAGGACAA
ATACTCAAGATTGAGTACAACGATAGTATTTAGAGCTAGATGGTAGGGGCTAATTCATCACTCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1404 bp | 467 aa | 130 | 1533 | + | No |
Chemistry : DDE
ORF sequence :
MYASKVVQLSIEAFGEANGVKLSPDNRWVQAATLIPWPELEEAYKTVFPSNKGRAGKPFRLLFGAHLIKTREGLSDRELVDAIRDVPAYQFFMGLPEYQP
RRPFVPSAMAHFRKRIAPIAELLENIIGDWTRKQLVGSVDDKVVILDATVTPVNIKYPQDYVLLGQARRNTEAMVTDLAHKLEVPIPRTYRREAERNYVH
FTKHPKRTRKATRGMVKSQLQYLRRNLRYIHEFQDQGGQLTSKQTQRLEVIETLYEQQDYMYRNNTHSVERRIISLSQPFVRPIKRGKAKPRNGTEFGPK
VDVSIIDGVVGIERFSFDNFNENQDFEDALYHYHDVHGEFPDMALVDKLYRTRENLRIAKNLGVKVCGPKLGRQPKYPDEASERKAKKEAREAENARGEI
ERNFALMKLKHGLGLVRAKTVETITTTVFTAMITANIDAVLRLFSLPFVLYVRYEGQILKIEYNDSI
RRPFVPSAMAHFRKRIAPIAELLENIIGDWTRKQLVGSVDDKVVILDATVTPVNIKYPQDYVLLGQARRNTEAMVTDLAHKLEVPIPRTYRREAERNYVH
FTKHPKRTRKATRGMVKSQLQYLRRNLRYIHEFQDQGGQLTSKQTQRLEVIETLYEQQDYMYRNNTHSVERRIISLSQPFVRPIKRGKAKPRNGTEFGPK
VDVSIIDGVVGIERFSFDNFNENQDFEDALYHYHDVHGEFPDMALVDKLYRTRENLRIAKNLGVKVCGPKLGRQPKYPDEASERKAKKEAREAENARGEI
ERNFALMKLKHGLGLVRAKTVETITTTVFTAMITANIDAVLRLFSLPFVLYVRYEGQILKIEYNDSI
Blast result :
Comments
ISLca3 is 68% aa similar to ISLca2.
References
1] ISfinder annotation (2010)
2] Makarova,K., Slesarev,A., Wolf,Y., Sorokin,A., Mirkin,B., Koonin,E., Pavlov,A., Pavlova,N., Karamychev,V., Polouchine,N., Shakhova,V., Grigoriev,I., Lou,Y., Rohksar,D., Lucas,S., Huang,K., Goodstein,D.M., Hawkins,T., Plengvidhya,V., Welker,D., Hughes,J., Goh,Y., Benson,A., Baldwin,K., Lee,J.H., Diaz-Muniz,I., Dosti,B., Smeianov,V., Wechter,W., Barabote,R., Lorca,G., Altermann,E., Barrangou,R., Ganesan,B., Xie,Y., Rawsthorne,H., Tamir,D., Parker,C., Breidt,F., Broadbent,J., Hutkins,R., O'Sullivan,D., Steele,J., Unlu,G., Saier,M., Klaenhammer,T., Richardson,P., Kozyavkin,S., Weimer,B. and Mills,D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15611-15616.
2] Makarova,K., Slesarev,A., Wolf,Y., Sorokin,A., Mirkin,B., Koonin,E., Pavlov,A., Pavlova,N., Karamychev,V., Polouchine,N., Shakhova,V., Grigoriev,I., Lou,Y., Rohksar,D., Lucas,S., Huang,K., Goodstein,D.M., Hawkins,T., Plengvidhya,V., Welker,D., Hughes,J., Goh,Y., Benson,A., Baldwin,K., Lee,J.H., Diaz-Muniz,I., Dosti,B., Smeianov,V., Wechter,W., Barabote,R., Lorca,G., Altermann,E., Barrangou,R., Ganesan,B., Xie,Y., Rawsthorne,H., Tamir,D., Parker,C., Breidt,F., Broadbent,J., Hutkins,R., O'Sullivan,D., Steele,J., Unlu,G., Saier,M., Klaenhammer,T., Richardson,P., Kozyavkin,S., Weimer,B. and Mills,D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15611-15616.