ISMich1
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_DS989841 | ND | Microcoleus chthonoplastes | Microcoleus chthonoplastes PCC 7420 |
DNA section
IS Length : 1670 bp
Ends
IR Length : 16/22
IRL : GGGCTTGCTGCACAAGACCGAAACCCTTAACCATCAATGCTTTGATCCGT
IRR : GGGTCTGCTGAATAAGTCGAAGAAACACAACTGATGGATTAAGTAGTTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TACTCAGAGA | TATTTA | GTGATATTGA | 6 |
| ATCTTAATCACAACTATTTC | TAAATTACAAGTCCTCCACC | 0 | |
| ACCAGTTCTG | TAAATA | GACCTCTTTC | 6 |
| GGGGCAGATT | TAAGTA | ATGCTAACCT | 6 |
DNA sequence
GGGCTTGCTGCACAAGACCGAAACCCTTAACCATCAATGCTTTGATCCGTATTTACAGTTGACTTGGTGCTCATGAATCTGGTAAAATTGCTCAAAATCC
TTGCATCATGATCAGGTAGATGTACCGAAAAGTTGAGAACGCCCCCAGGGAAGCCGAAGATTTTGAACTACCATTTGAAGGAAAGTTATCACAGGATAAC
CGCTGGGTAATCATGGCTTCTTTAGTACCTTGGGAGGAATTTGAAGAAGAATATGCCCAAAATTTTTCTGCGGAAATGGGAGCGCCAGCGCTACCATTTA
GGGTAGCACTGGGTTCATTAATAATCAAAGAAAAACTAGGAATAAGTGATAGAGAGACAGTCGAACAAATCAAAGAAAACCCTTACTTACAATACTTTAT
AGGTCAAAGGCATTACAGCAGCGAAGCTCCTTATGACTCATCACTATTGGCCAGATTCCGGGAAAGGATAAATGTGAATCTAGTCAACCGAATAAATGAG
AAAATGGTGAAGAAAAGTCAATCAGAGACGGATGAAGAAGCTAAAAAAAAAGTGCCGAAATCCAAAGTCAAGAAAGAAGAGAGTCAGCCAATAAAGGACA
ACTAATAGTTGATGCAAGCTGTGTGCCAGGAGATATAAGTTATCCCAATGATTTGGGGATATTAAATAAAGCTAGGGTCAAAACCGAAAAAATAATAGAC
AGTCTATATGAACCCCTGAAGGATCAACTAAATAAAAAACCAAAAACTTATAGAAATAGAGCCAGAAAGAATTACTTAAAAGTAGCAAAGAAAAAGAGAC
AAACAAGAAAAGAAATAAGAAAAGCAATAAAAAAACAACTGCAATACATAAAGAGAAACTTGGGGAGTATAGACGCGCTAGTTAAGGCAGGGTCTAGTCT
TGGGGCATTGAGTCGTACTGAATATAAGAGCCTGTTGGTAGTTAGCGAAATCTATCGTCAGCAAGAATGGATGTACAATAATAAAGTTCATAGAATTGAG
CATAGAATAGTAAATGTGAGTCAAGCCCATATCCGTCCAATAGTTAGAGGCAAAGCGGGAGTTGCCGTAGAATTTGGAGCTAAAATATCGGCAAGTGTAA
GAGATGGATACGTATTTTTAGACCGTATCAGTTGGGATAACTTTAATGAATCTGTCGATTTAAAAGCTCAAATAGAAGCTTATTATAACTACACAGGATT
CTATCCAGAATCCGTTCATGTAGATAAAATCTATCGCAGTCGAGCCAATCGATCCTTCTGTAAAGAAAAAGGAATTAGAATCAGTGGCCCTCCCTTAGGA
AGACCGAAAGCCAATGTCAGTCAAGAATTAAAGAAACAAGCTCAAGAAGACGAGAGAATTCGCAATAATATTGAAGGAAAATTTGGCATATCAAAACGTA
GATATAGCCTAAGTCGCGTCATGGCTAAACTACCTCATACGTCCGAAACAGCTATTGCCATTACTTTCCTCGTCATGAATCTGTCTGCCCTGCTCAGGCA
GGTTTTTTGTCTTTTTTATATATATTCCACAATCAAGTCTTTTTCGGCGTCAATTCATTATTAAACCTTATAATTAAGCGAATCGTTAACAACAATAACT
TAAGTTCGCTGTCGTATTATATAACTACTTAATCCATCAGTTGTGTTTCTTCGACTTATTCAGCAGACCC
TTGCATCATGATCAGGTAGATGTACCGAAAAGTTGAGAACGCCCCCAGGGAAGCCGAAGATTTTGAACTACCATTTGAAGGAAAGTTATCACAGGATAAC
CGCTGGGTAATCATGGCTTCTTTAGTACCTTGGGAGGAATTTGAAGAAGAATATGCCCAAAATTTTTCTGCGGAAATGGGAGCGCCAGCGCTACCATTTA
GGGTAGCACTGGGTTCATTAATAATCAAAGAAAAACTAGGAATAAGTGATAGAGAGACAGTCGAACAAATCAAAGAAAACCCTTACTTACAATACTTTAT
AGGTCAAAGGCATTACAGCAGCGAAGCTCCTTATGACTCATCACTATTGGCCAGATTCCGGGAAAGGATAAATGTGAATCTAGTCAACCGAATAAATGAG
AAAATGGTGAAGAAAAGTCAATCAGAGACGGATGAAGAAGCTAAAAAAAAAGTGCCGAAATCCAAAGTCAAGAAAGAAGAGAGTCAGCCAATAAAGGACA
ACTAATAGTTGATGCAAGCTGTGTGCCAGGAGATATAAGTTATCCCAATGATTTGGGGATATTAAATAAAGCTAGGGTCAAAACCGAAAAAATAATAGAC
AGTCTATATGAACCCCTGAAGGATCAACTAAATAAAAAACCAAAAACTTATAGAAATAGAGCCAGAAAGAATTACTTAAAAGTAGCAAAGAAAAAGAGAC
AAACAAGAAAAGAAATAAGAAAAGCAATAAAAAAACAACTGCAATACATAAAGAGAAACTTGGGGAGTATAGACGCGCTAGTTAAGGCAGGGTCTAGTCT
TGGGGCATTGAGTCGTACTGAATATAAGAGCCTGTTGGTAGTTAGCGAAATCTATCGTCAGCAAGAATGGATGTACAATAATAAAGTTCATAGAATTGAG
CATAGAATAGTAAATGTGAGTCAAGCCCATATCCGTCCAATAGTTAGAGGCAAAGCGGGAGTTGCCGTAGAATTTGGAGCTAAAATATCGGCAAGTGTAA
GAGATGGATACGTATTTTTAGACCGTATCAGTTGGGATAACTTTAATGAATCTGTCGATTTAAAAGCTCAAATAGAAGCTTATTATAACTACACAGGATT
CTATCCAGAATCCGTTCATGTAGATAAAATCTATCGCAGTCGAGCCAATCGATCCTTCTGTAAAGAAAAAGGAATTAGAATCAGTGGCCCTCCCTTAGGA
AGACCGAAAGCCAATGTCAGTCAAGAATTAAAGAAACAAGCTCAAGAAGACGAGAGAATTCGCAATAATATTGAAGGAAAATTTGGCATATCAAAACGTA
GATATAGCCTAAGTCGCGTCATGGCTAAACTACCTCATACGTCCGAAACAGCTATTGCCATTACTTTCCTCGTCATGAATCTGTCTGCCCTGCTCAGGCA
GGTTTTTTGTCTTTTTTATATATATTCCACAATCAAGTCTTTTTCGGCGTCAATTCATTATTAAACCTTATAATTAAGCGAATCGTTAACAACAATAACT
TAAGTTCGCTGTCGTATTATATAACTACTTAATCCATCAGTTGTGTTTCTTCGACTTATTCAGCAGACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 486 bp | 161 aa | 120 | 605 | + | No |
Description : First part of the transposase
ORF sequence :
MYRKVENAPREAEDFELPFEGKLSQDNRWVIMASLVPWEEFEEEYAQNFSAEMGAPALPFRVALGSLIIKEKLGISDRETVEQIKENPYLQYFIGQRHYS
SEAPYDSSLLARFRERINVNLVNRINEKMVKKSQSETDEEAKKKVPKSKVKKEESQPIKDN
SEAPYDSSLLARFRERINVNLVNRINEKMVKKSQSETDEEAKKKVPKSKVKKEESQPIKDN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 920 bp | 339 aa | 645 | 1564 | + | No |
Description : Second part of the transposase
ORF sequence :
KKSAEIQSQERRESANKGQLIVDASCVPGDISYPNDLGILNKARVKTEKIIDSLYEPLKDQLNKKPKTYRNRARKNYLKVAKKKRQTRKEIRKAIKKQLQ
YIKRNLGSIDALVKAGSSLGALSRTEYKSLLVVSEIYRQQEWMYNNKVHRIEHRIVNVSQAHIRPIVRGKAGVAVEFGAKISASVRDGYVFLDRISWDNF
NESVDLKAQIEAYYNYTGFYPESVHVDKIYRSRANRSFCKEKGIRISGPPLGRPKANVSQELKKQAQEDERIRNNIEGKFGISKRRYSLSRVMAKLPHTS
ETAIAITFLVMNLSALLRQVFCLFYIYSTIKSFSASIHY
YIKRNLGSIDALVKAGSSLGALSRTEYKSLLVVSEIYRQQEWMYNNKVHRIEHRIVNVSQAHIRPIVRGKAGVAVEFGAKISASVRDGYVFLDRISWDNF
NESVDLKAQIEAYYNYTGFYPESVHVDKIYRSRANRSFCKEKGIRISGPPLGRPKANVSQELKKQAQEDERIRNNIEGKFGISKRRYSLSRVMAKLPHTS
ETAIAITFLVMNLSALLRQVFCLFYIYSTIKSFSASIHY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1445 bp | 480 aa | 120 | 1564 | + | Yes |
Chemistry : DDE
ORF sequence :
MYRKVENAPREAEDFELPFEGKLSQDNRWVIMASLVPWEEFEEEYAQNFSAEMGAPALPFRVALGSLIIKEKLGISDRETVEQIKENPYLQYFIGQRHYS
SEAPYDSSLLARFRERINVNLVNRINEKMVKKSQSETDEEAKKSAEIQSQERRESANKGQLIVDASCVPGDISYPNDLGILNKARVKTEKIIDSLYEPLK
DQLNKKPKTYRNRARKNYLKVAKKKRQTRKEIRKAIKKQLQYIKRNLGSIDALVKAGSSLGALSRTEYKSLLVVSEIYRQQEWMYNNKVHRIEHRIVNVS
QAHIRPIVRGKAGVAVEFGAKISASVRDGYVFLDRISWDNFNESVDLKAQIEAYYNYTGFYPESVHVDKIYRSRANRSFCKEKGIRISGPPLGRPKANVS
QELKKQAQEDERIRNNIEGKFGISKRRYSLSRVMAKLPHTSETAIAITFLVMNLSALLRQVFCLFYIYSTIKSFSASIHY
SEAPYDSSLLARFRERINVNLVNRINEKMVKKSQSETDEEAKKSAEIQSQERRESANKGQLIVDASCVPGDISYPNDLGILNKARVKTEKIIDSLYEPLK
DQLNKKPKTYRNRARKNYLKVAKKKRQTRKEIRKAIKKQLQYIKRNLGSIDALVKAGSSLGALSRTEYKSLLVVSEIYRQQEWMYNNKVHRIEHRIVNVS
QAHIRPIVRGKAGVAVEFGAKISASVRDGYVFLDRISWDNFNESVDLKAQIEAYYNYTGFYPESVHVDKIYRSRANRSFCKEKGIRISGPPLGRPKANVS
QELKKQAQEDERIRNNIEGKFGISKRRYSLSRVMAKLPHTSETAIAITFLVMNLSALLRQVFCLFYIYSTIKSFSASIHY
Blast result :
Comments
ISMich1 is 78% aa similar to ISMae6. The third ORF is a putative transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2010)
2] Tandeau de Marsac,N., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2008) Direct submission GenBank.
2] Tandeau de Marsac,N., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2008) Direct submission GenBank.