ISMafe1
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AATS01000020 | ND | Mariprofundus ferrooxydans | Mariprofundus ferrooxydans PV-1 |
DNA section
IS Length : 1745 bp
Ends
IR Length : 12/15
IRL : GATTCCCCTCAAAAAGTCACAACCCATTGATATATTGAGACAAAGCGATG
IRR : GAGCCTCCTCAAAAACATCCATCTACCATCCAGTGGAAGCGTCCGCGAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCCTCGGCA | TAAATA | TTTTAGCGGC | 6 |
DNA sequence
GATTCCCCTCAAAAAGTCACAACCCATTGATATATTGAGACAAAGCGATGTTCATGGTAGTCTGAAGTGCAAGGGAATGGGCGCATCTGGCGCAAAAGGT
TTGCACATGATACGAGAGACAAGTGACCGGCAACTTCAGTTGAAGGACTTTATTCATCCATTTGGATGTGATTTGAATTCTGAGAATCGTTGGGTGAAGC
TGGCGGAGGTTGTGCCTTGGGATGATCTTGCCGTGGCTTACAATTCCGTGATGAGCGCGACGCAGGGTCGGCCTGCGAAGCCTGCGCGTCTGGTGATCGG
TGCGCTGATCATCAAGCACAAGCTGAACCTGAGCGATGAAGAGACAGTATTGCAGATCCAGGAGAACCCCTATCTCCAGTTTTTTTGCGGCCTTGGTGCA
TATGTGGATGCACCGCCGTTTGTTCCGAGCCTGTTCGTGGAGATACGCAAGCGCATGGGTGCGGAAGTGTTTGATGCGTTTGAGCAGGCGATAGTGGATC
GGGCGGTGCATATCCAAAAGAAGCGGAAACCAACCCGGGTCAGCACGTCCGGCGGTGATGGGTCACCTCCTTCTTCCTGCGGGGATGGGTCCGACCAGGA
TCGACCGGCACCGGTTGATGCGGTTGATGCGCAGGCAGAGGACGTGACACACCATGGCAAGCTGCTGATGGATGCGACGGTGGCCGACCAGATGATCCGC
TTTCCGACGGATCTCGGCCTGCTCAATGAGGCGCGTGAGATCTCCGAGTCCTTGATTGATGAGCTGCACAAACAAACCGGCGGCAAGAAGCCGCGCACTT
ACAGGCAGCAGGCGCGCAAGGTATTTCTTTCCATCAGCAAGCGCAAGAAACCGGGCGGCAAGCTGATCCGCAAGGGCATCCGCCAGCAGTTGCAGTATGT
GCGCCGCAATCTTGGGCATATTGAGCGGATGCTGGACGAGCTGATGGCGGAGGCCGGCATGCCGTTTCCCTTAGGACACAAGCTTCAACGCCAGTACTGG
ATTATTCAGCATGTGTATGCGCAGCAGAAGCGGATGTATGAGACGAAGAGCCGCCGCTGTGATGACCGTATTGTCAGCATCAGTCAGCCGCATGTGCGGC
CGATTGTACGGGGCAAGGCTGGCCGCCCGGTGGAGTTTGGCGCAAAGGTGAGCGTCAGCATGATTGGCAAACTGGCATTTGTAGATCATCTGGATTGGGA
TGCATTCAATGAGTCACAGGATCTGATCGCACAGGTGGAGCGATACAAGGCGCGCTTCGGCTTTTATCCTGAGGTGGTGCTGGCTGATGGCATCTACGGC
ACACGCGCGAATCGGCGGTGGCTGAAGGAGCATGGCATCCGCTTTGGCGGCAGGCCTTTGGGCAGGCCAAGGAAGGTTACATCGGAGAATATGGATGAGA
TCAAACAGCTCAAGCGCCAGCGCAGGGAGGATGAGCGCAGTCGCATCCCTGTCGAAGGCAAGTTCGGGCAAGGGAAAAACGGTTACCGGCTCAATCAAAT
ACGCGCCCGTTTGGCTTCAACCTCTGAGGCATGGGTGCGGAGCATCTTCCTGGTGATGAACCTGATTGCCCTGCTCAGGTTTTTGCTGCCTTTTTTGCGC
CCATCAGCCATCATGGATGCCTGCACAACATGGCTCAGGGGCATGCTGAGGCTTGATGACGGCGTTGGCGATCGTTTGACTGGCATGCATCCCCGCTTCG
CGGACGCTTCCACTGGATGGTAGATGGATGTTTTTGAGGAGGCTC
TTGCACATGATACGAGAGACAAGTGACCGGCAACTTCAGTTGAAGGACTTTATTCATCCATTTGGATGTGATTTGAATTCTGAGAATCGTTGGGTGAAGC
TGGCGGAGGTTGTGCCTTGGGATGATCTTGCCGTGGCTTACAATTCCGTGATGAGCGCGACGCAGGGTCGGCCTGCGAAGCCTGCGCGTCTGGTGATCGG
TGCGCTGATCATCAAGCACAAGCTGAACCTGAGCGATGAAGAGACAGTATTGCAGATCCAGGAGAACCCCTATCTCCAGTTTTTTTGCGGCCTTGGTGCA
TATGTGGATGCACCGCCGTTTGTTCCGAGCCTGTTCGTGGAGATACGCAAGCGCATGGGTGCGGAAGTGTTTGATGCGTTTGAGCAGGCGATAGTGGATC
GGGCGGTGCATATCCAAAAGAAGCGGAAACCAACCCGGGTCAGCACGTCCGGCGGTGATGGGTCACCTCCTTCTTCCTGCGGGGATGGGTCCGACCAGGA
TCGACCGGCACCGGTTGATGCGGTTGATGCGCAGGCAGAGGACGTGACACACCATGGCAAGCTGCTGATGGATGCGACGGTGGCCGACCAGATGATCCGC
TTTCCGACGGATCTCGGCCTGCTCAATGAGGCGCGTGAGATCTCCGAGTCCTTGATTGATGAGCTGCACAAACAAACCGGCGGCAAGAAGCCGCGCACTT
ACAGGCAGCAGGCGCGCAAGGTATTTCTTTCCATCAGCAAGCGCAAGAAACCGGGCGGCAAGCTGATCCGCAAGGGCATCCGCCAGCAGTTGCAGTATGT
GCGCCGCAATCTTGGGCATATTGAGCGGATGCTGGACGAGCTGATGGCGGAGGCCGGCATGCCGTTTCCCTTAGGACACAAGCTTCAACGCCAGTACTGG
ATTATTCAGCATGTGTATGCGCAGCAGAAGCGGATGTATGAGACGAAGAGCCGCCGCTGTGATGACCGTATTGTCAGCATCAGTCAGCCGCATGTGCGGC
CGATTGTACGGGGCAAGGCTGGCCGCCCGGTGGAGTTTGGCGCAAAGGTGAGCGTCAGCATGATTGGCAAACTGGCATTTGTAGATCATCTGGATTGGGA
TGCATTCAATGAGTCACAGGATCTGATCGCACAGGTGGAGCGATACAAGGCGCGCTTCGGCTTTTATCCTGAGGTGGTGCTGGCTGATGGCATCTACGGC
ACACGCGCGAATCGGCGGTGGCTGAAGGAGCATGGCATCCGCTTTGGCGGCAGGCCTTTGGGCAGGCCAAGGAAGGTTACATCGGAGAATATGGATGAGA
TCAAACAGCTCAAGCGCCAGCGCAGGGAGGATGAGCGCAGTCGCATCCCTGTCGAAGGCAAGTTCGGGCAAGGGAAAAACGGTTACCGGCTCAATCAAAT
ACGCGCCCGTTTGGCTTCAACCTCTGAGGCATGGGTGCGGAGCATCTTCCTGGTGATGAACCTGATTGCCCTGCTCAGGTTTTTGCTGCCTTTTTTGCGC
CCATCAGCCATCATGGATGCCTGCACAACATGGCTCAGGGGCATGCTGAGGCTTGATGACGGCGTTGGCGATCGTTTGACTGGCATGCATCCCCGCTTCG
CGGACGCTTCCACTGGATGGTAGATGGATGTTTTTGAGGAGGCTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1647 bp | 548 aa | 77 | 1723 | + | No |
Chemistry : DDE
ORF sequence :
MGASGAKGLHMIRETSDRQLQLKDFIHPFGCDLNSENRWVKLAEVVPWDDLAVAYNSVMSATQGRPAKPARLVIGALIIKHKLNLSDEETVLQIQENPYL
QFFCGLGAYVDAPPFVPSLFVEIRKRMGAEVFDAFEQAIVDRAVHIQKKRKPTRVSTSGGDGSPPSSCGDGSDQDRPAPVDAVDAQAEDVTHHGKLLMDA
TVADQMIRFPTDLGLLNEAREISESLIDELHKQTGGKKPRTYRQQARKVFLSISKRKKPGGKLIRKGIRQQLQYVRRNLGHIERMLDELMAEAGMPFPLG
HKLQRQYWIIQHVYAQQKRMYETKSRRCDDRIVSISQPHVRPIVRGKAGRPVEFGAKVSVSMIGKLAFVDHLDWDAFNESQDLIAQVERYKARFGFYPEV
VLADGIYGTRANRRWLKEHGIRFGGRPLGRPRKVTSENMDEIKQLKRQRREDERSRIPVEGKFGQGKNGYRLNQIRARLASTSEAWVRSIFLVMNLIALL
RFLLPFLRPSAIMDACTTWLRGMLRLDDGVGDRLTGMHPRFADASTGW
QFFCGLGAYVDAPPFVPSLFVEIRKRMGAEVFDAFEQAIVDRAVHIQKKRKPTRVSTSGGDGSPPSSCGDGSDQDRPAPVDAVDAQAEDVTHHGKLLMDA
TVADQMIRFPTDLGLLNEAREISESLIDELHKQTGGKKPRTYRQQARKVFLSISKRKKPGGKLIRKGIRQQLQYVRRNLGHIERMLDELMAEAGMPFPLG
HKLQRQYWIIQHVYAQQKRMYETKSRRCDDRIVSISQPHVRPIVRGKAGRPVEFGAKVSVSMIGKLAFVDHLDWDAFNESQDLIAQVERYKARFGFYPEV
VLADGIYGTRANRRWLKEHGIRFGGRPLGRPRKVTSENMDEIKQLKRQRREDERSRIPVEGKFGQGKNGYRLNQIRARLASTSEAWVRSIFLVMNLIALL
RFLLPFLRPSAIMDACTTWLRGMLRLDDGVGDRLTGMHPRFADASTGW
Blast result :
Comments
ISMafe1 is 62% aa similar to ISAcma17.
References
1] ISfinder annotation (2010)
2] Emerson,D., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.
2] Emerson,D., Ferriera,S., Johnson,J., Kravitz,S., Halpern,A., Remington,K., Beeson,K., Tran,B., Rogers,Y.-H., Friedman,R. and Venter,J.C. (2006) Direct submission GenBank.