ISCysp18
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010547 | ND | Cyanothece sp. | Cyanothece sp. ATCC 51142 |
DNA section
IS Length : 1702 bp
Ends
IR Length : 15/16
IRL : GGGTTTGCTGAAAAAGGGCTAGAATAGTTATCTTCTAAAGGTTACAGCCC
IRR : GGCTTTGCTGAAAAAGTCACTCAAAAATTTATGCGGCCAAAAGCCAGTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCAACCGTTCTAAACGA | TAACTAATAGTTCATC | 0 |
DNA sequence
GGGTTTGCTGAAAAAGGGCTAGAATAGTTATCTTCTAAAGGTTACAGCCCGATCTAGTGAACGCAAAAGTGCAACAAAAAACAGTCGAAACTCCCCAAAA
AGCCCTAAAATTGGTAAGAGCCTGTTGCACCTGGTTGACTAAAACAAATGTACCGCAAAAAAGAGCGATCGCAGATCACCCCAGAAAAATTTGAATTATT
GCCCCTGACCAAGTTATCGCCAGAAAATCGTTGGGTGATCATGGCCGAGCTAATACCCTGGGAAGAATTTGAAGAAGAGTATGCGAAACAATTTAAAATA
GAAAAAGGCGCACCAGCAAAACCATTTAAGATGGCGTTAGGCGCACTGATAATCAAAGAACAACTGGGGATAAGCGATAGAGAAACCGTAGAGCAAATAA
AAGAAAATCCATATTTACAATATTTTATCGGATTGGAAGCCTATACATTTGAGCCTCCCTTTGAAGCCTCAATGATGGTACATTTTAGACAAAGGATAAA
TGTAGATTTAGTAAAGAGGATTAATGAAGAAATGGTAAAAAGAGGGAGAGAACGAATCGAGCAGAAGTCCGAAAAAAAGGGGGTAGAAGAAGAAGAAACA
GGAGAAAGGAAAAATAAAGGCAAACTGATCTTGGATGCCACCTGCGCCCCTGGAGATATCAAGTATCCGACGGATTTGGAAATCTTAAATCAAGGAAGAA
AAGCAACTGAACAGATAATAGATACTCTCTATAAATCGATAAAAGGAAAATTAAAGAAAAAGCCCAGAACTTATCGAAATATAGCAAGAATTGAGTATTT
AAGAATAGTAAAAAACCGTCGTTCTACTAAAAAAGAGAGAAGAGAAGCGATTAGAAAACAACTTAAGTATATTGAAAAGAATCTAGTCAGTATAGAGAAA
CTAATCTTAGAGGGAGCGACCTTAAATAGTCTAAATAAAAGACAAAGAAATCTTCTAGAAGCTGTCAAAAAAGTCCATGAACAGCAACTCCAGATGTGGG
AAAAAGAAACCAAAAGTGTTCCCGAAAGAATTGTCAGTATAACTCAACCGCATATTCGACCTATCGTGAGAGGAAAAGCAGGGAAACCCACAGAGTTTGG
AGCCAAACTCTCAGTAAGTTATGTAGATAATTATATATTTCTAGACCGAATAAGTTGGGAAAATTTTAATGAATCTGGAGACTTGCAAGAACAAATTGAA
AAGTTTAAACAGACTTATGGTTGTTATCCATTCTCAGTTCATGCAGATAGAATTTATCGAAGTAAAGCCAATAAAGATTGGTGTAAACAAAGAGGAATTA
GACTCAGTGGAATAGGCTTAGGAAGACCTCCTAAAAACATCAGTCAAGAAGAGAGAAAGCAAACTAGAATAGACGAAGCATTTAGGAATAGAATTGAAGG
AAAATTTGGACAAGCAAAGAGAAAATTTAGTCTAGATTTGGTTAAAACTAAGCTTAAAGAAACTTCAGAAACTGCTATTGCTATGACCTTTTTAGTAGTT
AATCTTTGCCAACTGCTTCGGCAGATATTATTGTTGCTTTTTTCATTTTCTTTTATTAGCAAAACATTTGAACTAGATGAACAGATTATGATAATGAAAA
CTTATTTTATCAATCTTTTTACAGCAGTTAAAGTTATTGATTTAGAGGTAAATTACTGGCTTTTGGCCGCATAAATTTTTGAGTGACTTTTTCAGCAAAG
CC
AGCCCTAAAATTGGTAAGAGCCTGTTGCACCTGGTTGACTAAAACAAATGTACCGCAAAAAAGAGCGATCGCAGATCACCCCAGAAAAATTTGAATTATT
GCCCCTGACCAAGTTATCGCCAGAAAATCGTTGGGTGATCATGGCCGAGCTAATACCCTGGGAAGAATTTGAAGAAGAGTATGCGAAACAATTTAAAATA
GAAAAAGGCGCACCAGCAAAACCATTTAAGATGGCGTTAGGCGCACTGATAATCAAAGAACAACTGGGGATAAGCGATAGAGAAACCGTAGAGCAAATAA
AAGAAAATCCATATTTACAATATTTTATCGGATTGGAAGCCTATACATTTGAGCCTCCCTTTGAAGCCTCAATGATGGTACATTTTAGACAAAGGATAAA
TGTAGATTTAGTAAAGAGGATTAATGAAGAAATGGTAAAAAGAGGGAGAGAACGAATCGAGCAGAAGTCCGAAAAAAAGGGGGTAGAAGAAGAAGAAACA
GGAGAAAGGAAAAATAAAGGCAAACTGATCTTGGATGCCACCTGCGCCCCTGGAGATATCAAGTATCCGACGGATTTGGAAATCTTAAATCAAGGAAGAA
AAGCAACTGAACAGATAATAGATACTCTCTATAAATCGATAAAAGGAAAATTAAAGAAAAAGCCCAGAACTTATCGAAATATAGCAAGAATTGAGTATTT
AAGAATAGTAAAAAACCGTCGTTCTACTAAAAAAGAGAGAAGAGAAGCGATTAGAAAACAACTTAAGTATATTGAAAAGAATCTAGTCAGTATAGAGAAA
CTAATCTTAGAGGGAGCGACCTTAAATAGTCTAAATAAAAGACAAAGAAATCTTCTAGAAGCTGTCAAAAAAGTCCATGAACAGCAACTCCAGATGTGGG
AAAAAGAAACCAAAAGTGTTCCCGAAAGAATTGTCAGTATAACTCAACCGCATATTCGACCTATCGTGAGAGGAAAAGCAGGGAAACCCACAGAGTTTGG
AGCCAAACTCTCAGTAAGTTATGTAGATAATTATATATTTCTAGACCGAATAAGTTGGGAAAATTTTAATGAATCTGGAGACTTGCAAGAACAAATTGAA
AAGTTTAAACAGACTTATGGTTGTTATCCATTCTCAGTTCATGCAGATAGAATTTATCGAAGTAAAGCCAATAAAGATTGGTGTAAACAAAGAGGAATTA
GACTCAGTGGAATAGGCTTAGGAAGACCTCCTAAAAACATCAGTCAAGAAGAGAGAAAGCAAACTAGAATAGACGAAGCATTTAGGAATAGAATTGAAGG
AAAATTTGGACAAGCAAAGAGAAAATTTAGTCTAGATTTGGTTAAAACTAAGCTTAAAGAAACTTCAGAAACTGCTATTGCTATGACCTTTTTAGTAGTT
AATCTTTGCCAACTGCTTCGGCAGATATTATTGTTGCTTTTTTCATTTTCTTTTATTAGCAAAACATTTGAACTAGATGAACAGATTATGATAATGAAAA
CTTATTTTATCAATCTTTTTACAGCAGTTAAAGTTATTGATTTAGAGGTAAATTACTGGCTTTTGGCCGCATAAATTTTTGAGTGACTTTTTCAGCAAAG
CC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1476 bp | 491 aa | 199 | 1674 | + | No |
Chemistry : DDE
ORF sequence :
MPLTKLSPENRWVIMAELIPWEEFEEEYAKQFKIEKGAPAKPFKMALGALIIKEQLGISDRETVEQIKENPYLQYFIGLEAYTFEPPFEASMMVHFRQRI
NVDLVKRINEEMVKRGRERIEQKSEKKGVEEEETGERKNKGKLILDATCAPGDIKYPTDLEILNQGRKATEQIIDTLYKSIKGKLKKKPRTYRNIARIEY
LRIVKNRRSTKKERREAIRKQLKYIEKNLVSIEKLILEGATLNSLNKRQRNLLEAVKKVHEQQLQMWEKETKSVPERIVSITQPHIRPIVRGKAGKPTEF
GAKLSVSYVDNYIFLDRISWENFNESGDLQEQIEKFKQTYGCYPFSVHADRIYRSKANKDWCKQRGIRLSGIGLGRPPKNISQEERKQTRIDEAFRNRIE
GKFGQAKRKFSLDLVKTKLKETSETAIAMTFLVVNLCQLLRQILLLLFSFSFISKTFELDEQIMIMKTYFINLFTAVKVIDLEVNYWLLAA
NVDLVKRINEEMVKRGRERIEQKSEKKGVEEEETGERKNKGKLILDATCAPGDIKYPTDLEILNQGRKATEQIIDTLYKSIKGKLKKKPRTYRNIARIEY
LRIVKNRRSTKKERREAIRKQLKYIEKNLVSIEKLILEGATLNSLNKRQRNLLEAVKKVHEQQLQMWEKETKSVPERIVSITQPHIRPIVRGKAGKPTEF
GAKLSVSYVDNYIFLDRISWENFNESGDLQEQIEKFKQTYGCYPFSVHADRIYRSKANKDWCKQRGIRLSGIGLGRPPKNISQEERKQTRIDEAFRNRIE
GKFGQAKRKFSLDLVKTKLKETSETAIAMTFLVVNLCQLLRQILLLLFSFSFISKTFELDEQIMIMKTYFINLFTAVKVIDLEVNYWLLAA
Blast result :
Comments
ISCysp18 is 93% aa similar to ISCysp17.
References
1] ISfinder annotation (2010)
2] Welsh,E.A., Liberton,M., Stockel,J., Loh,T., Elvitigala,T., Wang,C., Wollam,A., Fulton,R.S., Clifton,S.W., Jacobs,J.M., Aurora,R., Ghosh,B.K., Sherman,L.A., Smith,R.D., Wilson,R.K. and Pakrasi,H.B. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (39), 15094-15099.
2] Welsh,E.A., Liberton,M., Stockel,J., Loh,T., Elvitigala,T., Wang,C., Wollam,A., Fulton,R.S., Clifton,S.W., Jacobs,J.M., Aurora,R., Ghosh,B.K., Sherman,L.A., Smith,R.D., Wilson,R.K. and Pakrasi,H.B. (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (39), 15094-15099.