ISfinder Mirror Site

ISSac2

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007759	ND	Syntrophus aciditrophicus	Syntrophus aciditrophicus SB

DNA section

IS Length : 1595 bp

Ends

IR Length : 22/27

IRL : GTCCGTCCTGAAAAATACGGGCTGCAACCCGCATAAATAATGAAACTGTT
IRR : GTCCGTCCTGAAAAATAGGATGTGAAAAATTTTTTGGTTGATCGATTCTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CATAGGATGC	TAAATA	GGATATTGAC	6

DNA sequence

GTCCGTCCTGAAAAATACGGGCTGCAACCCGCATAAATAATGAAACTGTTCTTTGAAAACTGAAGAAAATTGTGGATTTGCAGTTGTTTCTACTCTGAAA
ATGTACTATAAGTGGCTGATATGAAAGTACATATTCCAGAAAGGCAACTTCTTATGTTCGAGACGCAGTTTTCGGATGTCCTCAACCCGGAGCATGAGTT
ACTTCGTGCCGCACGCTTGATCGATTGGGACAACATCCATGATACACTGTCTGCTTACTATAGCCTCCGGGGTCGCCAGGGGAAATCCATTCGACTGATG
GTCGGCATACACATTTTGAAACATCGCTATAACTGTTCCGACGAACGAGCGGTTGAAATGCTGCATGAAAACGCCTACTGGCAGTGCTTTTGCGGATTCA
ACAGCTTTCAGCGTGGACAGATTATGGAAGCCACATCCCTGGTGAAGTTTCGGAATCGAATCGGCACGGAAGGGATGAAAGAGATAGAGGCGGCGTTGCT
CAAGACCTGGTCCGAGATGGGCTTGGTAAAAGCCAGACGGGTGGCGGTCGATACCACTGCTCAACCCAAGAACATCGCCTATCCGACGGACGCCGATCTC
CTTTACCGGATTAGAGAGAAGATCGTCAAGCAAGTCCAAAGGGTTCGTGAGGAGGTAACCCTTCGGAAGCCCTTTCGCACCTTCAATCGATCTGGTAAAA
AACTCCTCCTCGGAATCAAGAAGTTCCACCGAAAAAATCCGGAAGGCAGGAAAGAGGAGATCAAAAAACTCAAAGAGATGACCCGTCATGTGGTCGAAAA
GACCGGAAGGGTGGTAAGCAGTCTTTACCGTCGAGGGCTCAAGGAATCGGGACGGAAACTCAATCGTCTGGTTTCTCTCGGGAAGAAAATCGTCAATCAA
ACGGAGGAGGTTCTCTCCGGCCACCTTCCTCGAAAGCGGATTTACTCCCTTCATGAGCCGAATGTGGCCGTGATCAGGAAAGGAAAGAGCCACCCCGACA
CTGAGTTTGGAGCACTTATTGCTCTTGCAAAAAACGATGACGGTTTGATCCTGTCCCATGTGGAATATCAGCATAACGTCGCCGATGTGAAGACCTTGGG
ACGGGTGATCACCGGAATCAAGACCAATGTGGGACAACGACCACGAGAAGTTGCGGCGGATCGGGGATTCGATCAATCCCTCAAAAAACAGGAAAACTGC
CGCAGACGGTGGGGCATCAAAAGAATCGCCATTCCAAAGAAGGGAAAGACGCCTCATCCGGACAGCAAAGAATCCTGGTTCAGAAAAGCCTTGAAGCAAA
GAGTCAAGATTGAACCCGTTATCGGTCATCTCAAAAGCGATCATCGCATGAATCGCTGTCGGTACAAGGGAGCGGCAGGAGATACCGTCAATGTTGTATG
GGCCACCCTCGCCTGGAACATGAAAAAGATTGTCCAACTGCATGGGCAAATGGGATAAAAGCAAGCCTTAGAGGGAATCGACAGACAGTGTAGAGAAAAT
CATTCAGAAAATCTCTTCTTTAGCCGCAATCATCTTCAGTTTTCAAAGAATCGATCAACCAAAAAATTTTTCACATCCTATTTTTCAGGACGGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1347 bp	448 aa	112	1458	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MADMKVHIPERQLLMFETQFSDVLNPEHELLRAARLIDWDNIHDTLSAYYSLRGRQGKSIRLMVGIHILKHRYNCSDERAVEMLHENAYWQCFCGFNSFQ
RGQIMEATSLVKFRNRIGTEGMKEIEAALLKTWSEMGLVKARRVAVDTTAQPKNIAYPTDADLLYRIREKIVKQVQRVREEVTLRKPFRTFNRSGKKLLL
GIKKFHRKNPEGRKEEIKKLKEMTRHVVEKTGRVVSSLYRRGLKESGRKLNRLVSLGKKIVNQTEEVLSGHLPRKRIYSLHEPNVAVIRKGKSHPDTEFG
ALIALAKNDDGLILSHVEYQHNVADVKTLGRVITGIKTNVGQRPREVAADRGFDQSLKKQENCRRRWGIKRIAIPKKGKTPHPDSKESWFRKALKQRVKI
EPVIGHLKSDHRMNRCRYKGAAGDTVNVVWATLAWNMKKIVQLHGQMG

Blast result :

Comments

ISSac2 is 51% aa similar to ISVbsp2.

References

1] ISfinder annotation (2010)
2] McInerney,M.J., Rohlin,L., Mouttaki,H., Kim,U., Krupp,R.S., Rios-Hernandez,L., Sieber,J., Struchtemeyer,C.G., Bhattacharyya,A., Campbell,J.W. and Gunsalus,R.P. (2007) Proc. Natl. Acad. Sci. U.S.A. 104 (18), 7600-7605.