ISfinder Mirror Site

ISGpr5

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_DS999407	ND	Gamma proteobacterium	Gamma proteobacterium

DNA section

IS Length : 1558 bp

Ends

IR Length : 15/17

IRL : GTCGACCCTGAACAACTCGTTTGAGACTATGAATATAGGGTTTGCAGTGC
IRR : GTCGGCCCTGAACCACTTTCACCAATGCACAAGGCTGTGGCTGCTGGTCG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTGCCTACCGAGAT	TAAAAA	GATTGCTTACTGCA	6

DNA sequence

GTCGACCCTGAACAACTCGTTTGAGACTATGAATATAGGGTTTGCAGTGCATTTTGGCTCCCTGGAATCGCAAGGTTTTGGTAAAATGGGCCCAGTTTCT
TGCAATTTGGGGCCGCACCGTGAAACCCAAGCCAGATCCCGCGTCAAAGCGATGGCAGAATAAACTGTTCGATGACGAAGGCCCCGCATTGCTCGACTTC
ATTGACCGGAAACACCCCCTGGTCCGCATGGCGGACTCGATGCAGTGGGAACTGTTCGAGACCCACTGGAGCGCGCTGCACAGCGGCGCCGGCGGCCGCA
TGGCGAGTTCCGGTCGGCAAGTCGCGGGCCTTCTGATGCTCAAGCACATGGAGGCGCTGTCCGATGAGCGTCTCATGGAGGTATGGATTACCAATCCCTA
CTACCAGTACTTCTGCGGCGAAACACATTTCCAGCACCGGCCCCCGGTAGATCCGACGTCCATGACGAAATGGCGCAATCGTCTGGGCGAGGAAGGCATG
GAGTGGCTGCTGACGACGGTGGTCGAGAGCGCGACGCGCAGCGGTTTCGTCGAGCGCAGCAGCTTTGCGCACGTGAGTGCGGATTCCACCGTGATGGAAA
AGCACATCGCCCACCCCACGGACAGCGCGCTGCTGGAGAAGATGCGGTGCAAGTTAGTGGACTTCATGCGTGAGCATGATCTGTCGATCCGCCAAAGCTA
TTCCCGGAAAGGACCGCGGCTGGCACAGCAGATTGGTCGCCATGCGCATGCCAGGCAGTTCAAGCGCATGCGGCGCCTGCTGCGTAAGCAGCGGACCTGG
GTCGGCCGGTTACAACGGGAACTGGAGCGGCAGCTTGAAAGCTTGGCGCCGGTGGCTCGTGAACGGGCCGAGGAACTCATCCGTCTGGCAAAGCGGCTGA
TTGAGCAGGGTAAGAACCCGCAGACGAAGAACAAGCTCTATTCACTGCATGAGCAGGCCGTGGACTGTATCTCCAAGGGTAAGGCAAGGAAGCGCTACGA
GTTCGGCACCAAGGTCGGGATCGTGTGCACGCAGAAGGAGCAGTTCGTGGTCGGCATGCGCAGCTACCCTGGGAATCCTTACGACGGTCACACGCTGGAT
GACCTGCTGCAGCAGGCGGAGACGATCACGGCGATTCCCGTGAAAACCGTCGCGGTTGATCTTGGCTACCGGGGACGACACGGCACGCAGGCGCGGGTAA
TACACCGCGGCCGGAAGCTGAGCAAGCGCGAGAAGCAACGGCTGCGCCGCCGTTCGGCCCTGGAAGCGATCATCGGTCACATGAAAATGGATGGACTATT
GGAACGCTGCCATTTGAAGGGTACCCGCGGCGACGCGGTGCACGCGATCCTGTGCGGTATCGGTCATAACCTGAGACAGATGCGCGCCTGGTGGATGCGT
CGTCTTTTTGCGCTCTGGATTCAGTGGCTGGAGCAGCTGATATTGCCCCAAGGGGTCGCCCAGACTGCTATAGCAGCCTGAAACCGCAAAACCGTGAACT
GATCGAGCCGACCAGCAGCCACAGCCTTGTGCATTGGTGAAAGTGGTTCAGGGCCGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1362 bp	453 aa	120	1481	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPKPDPASKRWQNKLFDDEGPALLDFIDRKHPLVRMADSMQWELFETHWSALHSGAGGRMASSGRQVAGLLMLKHMEALSDERLMEVWITNPYYQYFCG
ETHFQHRPPVDPTSMTKWRNRLGEEGMEWLLTTVVESATRSGFVERSSFAHVSADSTVMEKHIAHPTDSALLEKMRCKLVDFMREHDLSIRQSYSRKGPR
LAQQIGRHAHARQFKRMRRLLRKQRTWVGRLQRELERQLESLAPVARERAEELIRLAKRLIEQGKNPQTKNKLYSLHEQAVDCISKGKARKRYEFGTKVG
IVCTQKEQFVVGMRSYPGNPYDGHTLDDLLQQAETITAIPVKTVAVDLGYRGRHGTQARVIHRGRKLSKREKQRLRRRSALEAIIGHMKMDGLLERCHLK
GTRGDAVHAILCGIGHNLRQMRAWWMRRLFALWIQWLEQLILPQGVAQTAIAA

Blast result :

Comments

ISGpr5 is 65% aa similar to ISRhsp1. There are two copies in the genome, and they form a possible composite transposon (length=8507 bp) with a DR of 6 bp.

References

1] Isfinder annotation (2010)
2] Amann,R., Fuchs,B., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2008) Direct submission Genbank.