ISRjo2
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008269 | ND | Rhodococcus jostii | Rhodococcus jostii RHA1 plasmid pRHL1 |
DNA section
IS Length : 1147 bp
Ends
IR Length : 15
IRL : GTGGTGTGTCTCGCAAATAATTCTGGTTCTCTTCGATAGAATCCCTATAT
IRR : GTGGTGTGTCTCGGATTTAGTTGATCTTTTCCAGTGCGACGCGGCCGCGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGAAGTAGC | TA | GTCTCCGGAC | 2 |
DNA sequence
GTGGTGTGTCTCGCAAATAATTCTGGTTCTCTTCGATAGAATCCCTATATGAGGCATCCGGTACCCGCCTTGGAGATTTCCGATGGGCAGCGCGTTTTGC
TTGAGTCGTTGGCGCGGTCGCAGTCGGGTGCACACCGTGAGGTGGTGCGGGCTAAGGCACTGTTGATGGCGTCCGAGGGTGTGGCCAACACGGCTATCGC
GCAGGCGTTGTCGGTATCGCCGGGCTCGGTGGCGAACTGGCGGGTGCGGTTCGCCGAGGACGGCATGGCGCGGTTGGGCGAGGTGCGCAAGGGTCGTGGT
CGCAAGCCCTCGATCCCGCAAGAGAAGATCGATGAGATCGTGGACCTGACGCAGAACTACCGCCCGAAAGGCGAGACTCATTGGAGTTGCCGGACGATGG
CCGAGGTGGTCGGGGTCTCGAAGGACACCGTCCAGCGGGTGTGGTCCGCTCGCGGACTCAAGCCGCACCGGGTCGAGACGTTCAAGCTGTCGAACGATCC
ACGATTCGAGGACAAACTCGTCGACGTCGTCGGTCTGTATCTGAACCCGCCCGAGCGGGCGATCGTGTTGTGCGCGGACGAGAAGTCGTCGGTGCAAGCC
CTCGACCGGACCCAGGCCTCGCTCCCGATGATCAAGGGCCGCGGCGAGACGATGACCCACGACTACAAGCGTCACGGCACCACCACCTTGTTCGCCGCCC
TCGACGTCCTCACCGGCACGGTGATCGGCCAGTGCCTGCCGCGCCACCGCCATCAGGAGTTCCTGAGATTTCTGCGCACCATTGACCGCGAGGTGCCCAA
GGACTTGGAAATCCACCTGATCCTGGACAACTACGCCACCCACAAGCACGAGAAGGTCCGGGCCTGGCTCGACAAGCATCCCCGGTTCCACCTGCACTTC
ACTCCCACCTCCTCCTCGTGGCTCAATCTCGTCGAACGTTGGTTCCGAGAGCTGACCGATAAAGCTCTGCGGCGCGGGGTATTTCACTCCGTACCGCACC
TCATCGCCTCGATCGAGGAATACCTCGACGCCCACAACGAAAACCCCCGACCGTACGAATGGACCGCGACCGCCGAATCCATCCTCGCCAAGGTCGCGCG
CGGCCGCGTCGCACTGGAAAAGATCAACTAAATCCGAGACACACCAC
TTGAGTCGTTGGCGCGGTCGCAGTCGGGTGCACACCGTGAGGTGGTGCGGGCTAAGGCACTGTTGATGGCGTCCGAGGGTGTGGCCAACACGGCTATCGC
GCAGGCGTTGTCGGTATCGCCGGGCTCGGTGGCGAACTGGCGGGTGCGGTTCGCCGAGGACGGCATGGCGCGGTTGGGCGAGGTGCGCAAGGGTCGTGGT
CGCAAGCCCTCGATCCCGCAAGAGAAGATCGATGAGATCGTGGACCTGACGCAGAACTACCGCCCGAAAGGCGAGACTCATTGGAGTTGCCGGACGATGG
CCGAGGTGGTCGGGGTCTCGAAGGACACCGTCCAGCGGGTGTGGTCCGCTCGCGGACTCAAGCCGCACCGGGTCGAGACGTTCAAGCTGTCGAACGATCC
ACGATTCGAGGACAAACTCGTCGACGTCGTCGGTCTGTATCTGAACCCGCCCGAGCGGGCGATCGTGTTGTGCGCGGACGAGAAGTCGTCGGTGCAAGCC
CTCGACCGGACCCAGGCCTCGCTCCCGATGATCAAGGGCCGCGGCGAGACGATGACCCACGACTACAAGCGTCACGGCACCACCACCTTGTTCGCCGCCC
TCGACGTCCTCACCGGCACGGTGATCGGCCAGTGCCTGCCGCGCCACCGCCATCAGGAGTTCCTGAGATTTCTGCGCACCATTGACCGCGAGGTGCCCAA
GGACTTGGAAATCCACCTGATCCTGGACAACTACGCCACCCACAAGCACGAGAAGGTCCGGGCCTGGCTCGACAAGCATCCCCGGTTCCACCTGCACTTC
ACTCCCACCTCCTCCTCGTGGCTCAATCTCGTCGAACGTTGGTTCCGAGAGCTGACCGATAAAGCTCTGCGGCGCGGGGTATTTCACTCCGTACCGCACC
TCATCGCCTCGATCGAGGAATACCTCGACGCCCACAACGAAAACCCCCGACCGTACGAATGGACCGCGACCGCCGAATCCATCCTCGCCAAGGTCGCGCG
CGGCCGCGTCGCACTGGAAAAGATCAACTAAATCCGAGACACACCAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1083 bp | 360 aa | 49 | 1131 | + | No |
Chemistry : DDE
ORF sequence :
MRHPVPALEISDGQRVLLESLARSQSGAHREVVRAKALLMASEGVANTAIAQALSVSPGSVANWRVRFAEDGMARLGEVRKGRGRKPSIPQEKIDEIVDL
TQNYRPKGETHWSCRTMAEVVGVSKDTVQRVWSARGLKPHRVETFKLSNDPRFEDKLVDVVGLYLNPPERAIVLCADEKSSVQALDRTQASLPMIKGRGE
TMTHDYKRHGTTTLFAALDVLTGTVIGQCLPRHRHQEFLRFLRTIDREVPKDLEIHLILDNYATHKHEKVRAWLDKHPRFHLHFTPTSSSWLNLVERWFR
ELTDKALRRGVFHSVPHLIASIEEYLDAHNENPRPYEWTATAESILAKVARGRVALEKIN
TQNYRPKGETHWSCRTMAEVVGVSKDTVQRVWSARGLKPHRVETFKLSNDPRFEDKLVDVVGLYLNPPERAIVLCADEKSSVQALDRTQASLPMIKGRGE
TMTHDYKRHGTTTLFAALDVLTGTVIGQCLPRHRHQEFLRFLRTIDREVPKDLEIHLILDNYATHKHEKVRAWLDKHPRFHLHFTPTSSSWLNLVERWFR
ELTDKALRRGVFHSVPHLIASIEEYLDAHNENPRPYEWTATAESILAKVARGRVALEKIN
Blast result :
Comments
ISRjo2 is 70% aa similar to ISCARN69.
References
1] ISfinder annotation (2010.
2] McLeod,M.P., Warren,R.L., Hsiao,W.W., Araki,N., Myhre,M., Fernandes,C., Miyazawa,D., Wong,W., Lillquist,A.L., Wang,D., Dosanjh,M., Hara,H., Petrescu,A., Morin,R.D., Yang,G., Stott,J.M., Schein,J.E., Shin,H., Smailus,D., Siddiqui,A.S., Marra,M.A., Jones,S.J., Holt,R., Brinkman,F.S., Miyauchi,K., Fukuda,M., Davies,J.E., Mohn,W.W. and Eltis,L.D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15582-15587.
2] McLeod,M.P., Warren,R.L., Hsiao,W.W., Araki,N., Myhre,M., Fernandes,C., Miyazawa,D., Wong,W., Lillquist,A.L., Wang,D., Dosanjh,M., Hara,H., Petrescu,A., Morin,R.D., Yang,G., Stott,J.M., Schein,J.E., Shin,H., Smailus,D., Siddiqui,A.S., Marra,M.A., Jones,S.J., Holt,R., Brinkman,F.S., Miyauchi,K., Fukuda,M., Davies,J.E., Mohn,W.W. and Eltis,L.D. (2006) Proc. Natl. Acad. Sci. U.S.A. 103 (42), 15582-15587.