ISOpb2
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_ABEA02000004 | ND | Opitutaceae bacterium | Opitutaceae bacterium TAV2 |
DNA section
IS Length : 1462 bp
Ends
IR Length : 14/15
IRL : GTCGGTGCTGAAGAACCATTTAATTTCCTATCAAGGAGCAATTTAACGCT
IRR : GTCGGTGCTGAGGAAGGGCGGTATTAAGCGAGTTTGAAGCATGGCGAGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGTAGTCAC | GAGATA | AGATTGACTG | 6 |
| CTGTATTTAA | TAATCCTA | GATTCCGCAG | 8 |
| ATCTGTAATC | TACATA | AAAAAAGAGG | 6 |
| GCACTCGGTC | TCGGTA | GGTTTAAGTT | 6 |
| TGGGTTAAGGAAACTAAGTA | TATGTCACTGTTTAGTCCCG | 0 | |
| ATTAAATAAAACACTAAGTA | TATGTAGTGTCATCAGCAGC | 0 |
DNA sequence
GTCGGTGCTGAAGAACCATTTAATTTCCTATCAAGGAGCAATTTAACGCTTGAGACTGGAGTGAAATTTTCCCAGAAAAGGGCAATTTATAAGGAAAATC
TGGGAAAAAGATGAAGCCAAAGGAAAACAAGCACGAGAGACAGAGGGAAATGTTCAAGATGGAGTTAATAATGTTGGTGAACATGAACCATCCGATGGTG
AAGCTGGCCGGGAATATAAACTGGGCCGAGTTTGACAAGCATTTGGGGCCGACGTATCATGAGAGGTTGGGGCAGCCCGGGTTGGGGACGAGGTTGATGG
TATCGTTACATTATTTGAAGTATCAATATGATCTGAGCGACGAAGAGGTGCTGGAGCATTGGGTGGAGAATCCATATTGGCAATATTTATCGGGGATGCA
GTATTTTGAGCATGAGAAGCCGATAGACTCGTCGAGCATGACGAGGTGGAGGAAGAGATTGGGGGAGAGTGGAGCCGAACAGATGCTCAAGCAGACGATA
GAGAGCGCGGTGAGGATGAAGGGGTTAAAGCCGGTGCAGGTGGTGCATATCAACGTTGACACGACGGTGCAAACCAAGGAGGTACGTTATCCGACCGATG
CGAGATCGTATGATCGGGCGAGGGAGAGGCTGGTGAAGGAAGCGCGGAAAGCCGGGCTGAAAGTGAAGCAGAGTTATGAACGGGTAGGGAGGGGATTATT
GATGATGGCCGGGCGCTATATGCATAGCCGAAAGATGAAGAGAGCGAAGGCATGTGTGAGGAAACTGAGGACGAATCTGGGCCGTGTGATACGAGAGATA
GAGAGACAAAAGCCTGTGGCGGCATTGCAAGGGTTGCTGGAGACGAGCAAGCAAATTTACAAACAAAAGACAGCAGACAAAAACAAAGTGTATAGTGTGC
ATGAGCCAAAGGTGGAATGCATATCGAAAGGGAAATCGGGAAAGAAATATGAATTTGGGCAGAAGGTAAGTGTGGCATCGACGAGCAAGGGAGGTTGGCT
GTTGGGAGCGTTATGTATGCCAGACAATCCGTATGATGGGCACACGCTTGAAGCGCAAATGGAGCAGGTAAAACGGCTCTATATTGAAAAACAGGGGCCG
AAAACGGCGCACGTGGACATGGGTTATCGAGGGCACAATTATGAGGGGCCGGTGGAGGTGATCGTGGACAAACGCAGGCGAGGGAAAATCCCCAAACGAG
TCTGGCGCTGGATGAAACGCCGTGCCGCCATTGAGCCGACAATCGGACATCTCAAGAATGAACATCGTATGGAGCGCAACAAGTTACGCGGATTGATCGG
AGACAAGGTCAATGCCATCCTCAGCGCCGCCGCGATGAACTTCGGAAAGCTCCTAGCTTTCCTTTTGGCCTTTTTTTCCCGCCTTCTGCTCGCGCTTTTT
CCCGCCTTGCGCACCTCGCCATGCTTCAAACTCGCTTAATACCGCCCTTCCTCAGCACCGAC
TGGGAAAAAGATGAAGCCAAAGGAAAACAAGCACGAGAGACAGAGGGAAATGTTCAAGATGGAGTTAATAATGTTGGTGAACATGAACCATCCGATGGTG
AAGCTGGCCGGGAATATAAACTGGGCCGAGTTTGACAAGCATTTGGGGCCGACGTATCATGAGAGGTTGGGGCAGCCCGGGTTGGGGACGAGGTTGATGG
TATCGTTACATTATTTGAAGTATCAATATGATCTGAGCGACGAAGAGGTGCTGGAGCATTGGGTGGAGAATCCATATTGGCAATATTTATCGGGGATGCA
GTATTTTGAGCATGAGAAGCCGATAGACTCGTCGAGCATGACGAGGTGGAGGAAGAGATTGGGGGAGAGTGGAGCCGAACAGATGCTCAAGCAGACGATA
GAGAGCGCGGTGAGGATGAAGGGGTTAAAGCCGGTGCAGGTGGTGCATATCAACGTTGACACGACGGTGCAAACCAAGGAGGTACGTTATCCGACCGATG
CGAGATCGTATGATCGGGCGAGGGAGAGGCTGGTGAAGGAAGCGCGGAAAGCCGGGCTGAAAGTGAAGCAGAGTTATGAACGGGTAGGGAGGGGATTATT
GATGATGGCCGGGCGCTATATGCATAGCCGAAAGATGAAGAGAGCGAAGGCATGTGTGAGGAAACTGAGGACGAATCTGGGCCGTGTGATACGAGAGATA
GAGAGACAAAAGCCTGTGGCGGCATTGCAAGGGTTGCTGGAGACGAGCAAGCAAATTTACAAACAAAAGACAGCAGACAAAAACAAAGTGTATAGTGTGC
ATGAGCCAAAGGTGGAATGCATATCGAAAGGGAAATCGGGAAAGAAATATGAATTTGGGCAGAAGGTAAGTGTGGCATCGACGAGCAAGGGAGGTTGGCT
GTTGGGAGCGTTATGTATGCCAGACAATCCGTATGATGGGCACACGCTTGAAGCGCAAATGGAGCAGGTAAAACGGCTCTATATTGAAAAACAGGGGCCG
AAAACGGCGCACGTGGACATGGGTTATCGAGGGCACAATTATGAGGGGCCGGTGGAGGTGATCGTGGACAAACGCAGGCGAGGGAAAATCCCCAAACGAG
TCTGGCGCTGGATGAAACGCCGTGCCGCCATTGAGCCGACAATCGGACATCTCAAGAATGAACATCGTATGGAGCGCAACAAGTTACGCGGATTGATCGG
AGACAAGGTCAATGCCATCCTCAGCGCCGCCGCGATGAACTTCGGAAAGCTCCTAGCTTTCCTTTTGGCCTTTTTTTCCCGCCTTCTGCTCGCGCTTTTT
CCCGCCTTGCGCACCTCGCCATGCTTCAAACTCGCTTAATACCGCCCTTCCTCAGCACCGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1329 bp | 442 aa | 111 | 1439 | + | No |
Chemistry : DDE
ORF sequence :
MKPKENKHERQREMFKMELIMLVNMNHPMVKLAGNINWAEFDKHLGPTYHERLGQPGLGTRLMVSLHYLKYQYDLSDEEVLEHWVENPYWQYLSGMQYFE
HEKPIDSSSMTRWRKRLGESGAEQMLKQTIESAVRMKGLKPVQVVHINVDTTVQTKEVRYPTDARSYDRARERLVKEARKAGLKVKQSYERVGRGLLMMA
GRYMHSRKMKRAKACVRKLRTNLGRVIREIERQKPVAALQGLLETSKQIYKQKTADKNKVYSVHEPKVECISKGKSGKKYEFGQKVSVASTSKGGWLLGA
LCMPDNPYDGHTLEAQMEQVKRLYIEKQGPKTAHVDMGYRGHNYEGPVEVIVDKRRRGKIPKRVWRWMKRRAAIEPTIGHLKNEHRMERNKLRGLIGDKV
NAILSAAAMNFGKLLAFLLAFFSRLLLALFPALRTSPCFKLA
HEKPIDSSSMTRWRKRLGESGAEQMLKQTIESAVRMKGLKPVQVVHINVDTTVQTKEVRYPTDARSYDRARERLVKEARKAGLKVKQSYERVGRGLLMMA
GRYMHSRKMKRAKACVRKLRTNLGRVIREIERQKPVAALQGLLETSKQIYKQKTADKNKVYSVHEPKVECISKGKSGKKYEFGQKVSVASTSKGGWLLGA
LCMPDNPYDGHTLEAQMEQVKRLYIEKQGPKTAHVDMGYRGHNYEGPVEVIVDKRRRGKIPKRVWRWMKRRAAIEPTIGHLKNEHRMERNKLRGLIGDKV
NAILSAAAMNFGKLLAFLLAFFSRLLLALFPALRTSPCFKLA
Blast result :
Comments
ISOpb2 is 66% aa similar to ISGlo4.
References
1] Isfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L., Schmidt,T. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Larimer,F., Land,M.L., Hauser,L., Schmidt,T. and Richardson,P. (2009) Direct submission GenBank.