ISPhae1
- Family IS5
- Group IS5
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| GQ868754 | Y | Paracoccus haeundaensis | Paracoccus haeundaensis LMG P-21903 |
DNA section
IS Length : 1485 bp
Ends
IR Length : 13/16
IRL : GTCCGGCCTGAACAACTTCTACTGCATTGATTTTGTGTGCTGAAGCTGCG
IRR : GTCGGCTCTGAACAACCGTGTCAGGCGGCTAATACTTCCCTGAGCAGGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCTAAACGA | TGATTA | CACACTGAAA | 6 |
DNA sequence
GTCCGGCCTGAACAACTTCTACTGCATTGATTTTGTGTGCTGAAGCTGCGGTTTTGTTGGCTTTGAATTGCAGGGTTTCGTATGCTTTTGCCAGAAACCC
TGCAATTTCCGGTCGCCGATGAAACCCCATTCCCGCGCTCCTGAACAGGATGACCTGCTCCGCCCACGACTGGTCGACATGATCGATGCGCGCCACGAGT
TGGTGAAGCTTGCGGCGCTGATCGACTGGGAGTTTTTCGAACGGGAATGGGCGACCTTCTTCCCTTCGCATCAGGGTCGACCCGCGACATCGCCACGCCT
GGTCGCGGGGCTCATGTATCTCCAGCACGCCTTCAAGCTCTCGGACGAGGCAGTCGTCGCCCGGTGGGTCGAGAACCCGTATTACCAACACTTCACTGGC
GAGACGTTCTTCCAGCACCGTTCTCCAATCGATCCCTCGTCGCTGGTGCGCTGGCGCAAGCGGATCGGCGAGGAAGGGGTGGAGTGGCTGCTGACCAAGA
CGATAGAGGCAGGCCAAGCTTCTGGCGCGGTCACCGACAAGAGCCTGAAACGGGTGGCCGTCGATACGACCGTGATGGAAAAGACCATCGCTCATCCCAC
GGATGCGCGGCTTTACGAGCGCGCCCGCGCCCTGCTGGCGGGCTTGGCGAAAGAAGCCGGGGTCGATCTGCGCCAGAGCTACGCCCGCCTTGCCCCGCGG
CTGGCCGCCCAAGTCGGACGGTATGCCCATGCCCGGCAGTTCAAGCGCATGCGCAAGGCCCTGCGCCAGCTCAAGGGCTATGTTGGCCGAGTGCGCCGGG
ACCTGCGCCGCTACCTCCAGGACATCCCCGAAGGCGCGCTGCGCGGACGGGTGCTGGAGGCGCTCTGGCTGGTCGGTCGCCTGCTCGAACAGACACCGAA
GAGCAAGAACAAGATCTATGCCCTGCACGAGCCGGAGGTCGACTGCATCTCCAAGGGCAAGGCGCGGGTCCGATACGAATTCGGCACCAAGGTCAGCCTC
GCCACCACTCTCGATGGGGGCTTTGTCGTTGGTGCTCGGAGCTTTCCTGGCAACCCCTACGACGGCCACACCCTGGCGTCAGCACTGGAGCAAGTCGCCA
TCCTGACCGACCAGGTGCCGGCTCTGGCTGTGGTCGACCGCGGCTATCGCGGCCATGGCGTGGAGGCCACCAAGGTCCTGATCAGCGGCACAAGACGCGG
CATTACCCCGCTCCTGGCACGGCTCCTCAAGCGACGAAGCGCCATCGAGCCTGAGATCGGCCACATGAAGAGCGATGGTCGCCTAGCGAGATGCCCGCTG
AAAGGCCGCATCGGCGACGCGGTCTTCGCCGTCCTCTGCGCCTGCGGCCACAATATCCGTAAGATCCTCGCCCATCTCAGGGCGCTTTGGACCCTATTGC
TTCACCTGATTGCGGCGAGGATTTGGCCCGAAAGGACCCTGCTCAGGGAAGTATTAGCCGCCTGACACGGTTGTTCAGAGCCGAC
TGCAATTTCCGGTCGCCGATGAAACCCCATTCCCGCGCTCCTGAACAGGATGACCTGCTCCGCCCACGACTGGTCGACATGATCGATGCGCGCCACGAGT
TGGTGAAGCTTGCGGCGCTGATCGACTGGGAGTTTTTCGAACGGGAATGGGCGACCTTCTTCCCTTCGCATCAGGGTCGACCCGCGACATCGCCACGCCT
GGTCGCGGGGCTCATGTATCTCCAGCACGCCTTCAAGCTCTCGGACGAGGCAGTCGTCGCCCGGTGGGTCGAGAACCCGTATTACCAACACTTCACTGGC
GAGACGTTCTTCCAGCACCGTTCTCCAATCGATCCCTCGTCGCTGGTGCGCTGGCGCAAGCGGATCGGCGAGGAAGGGGTGGAGTGGCTGCTGACCAAGA
CGATAGAGGCAGGCCAAGCTTCTGGCGCGGTCACCGACAAGAGCCTGAAACGGGTGGCCGTCGATACGACCGTGATGGAAAAGACCATCGCTCATCCCAC
GGATGCGCGGCTTTACGAGCGCGCCCGCGCCCTGCTGGCGGGCTTGGCGAAAGAAGCCGGGGTCGATCTGCGCCAGAGCTACGCCCGCCTTGCCCCGCGG
CTGGCCGCCCAAGTCGGACGGTATGCCCATGCCCGGCAGTTCAAGCGCATGCGCAAGGCCCTGCGCCAGCTCAAGGGCTATGTTGGCCGAGTGCGCCGGG
ACCTGCGCCGCTACCTCCAGGACATCCCCGAAGGCGCGCTGCGCGGACGGGTGCTGGAGGCGCTCTGGCTGGTCGGTCGCCTGCTCGAACAGACACCGAA
GAGCAAGAACAAGATCTATGCCCTGCACGAGCCGGAGGTCGACTGCATCTCCAAGGGCAAGGCGCGGGTCCGATACGAATTCGGCACCAAGGTCAGCCTC
GCCACCACTCTCGATGGGGGCTTTGTCGTTGGTGCTCGGAGCTTTCCTGGCAACCCCTACGACGGCCACACCCTGGCGTCAGCACTGGAGCAAGTCGCCA
TCCTGACCGACCAGGTGCCGGCTCTGGCTGTGGTCGACCGCGGCTATCGCGGCCATGGCGTGGAGGCCACCAAGGTCCTGATCAGCGGCACAAGACGCGG
CATTACCCCGCTCCTGGCACGGCTCCTCAAGCGACGAAGCGCCATCGAGCCTGAGATCGGCCACATGAAGAGCGATGGTCGCCTAGCGAGATGCCCGCTG
AAAGGCCGCATCGGCGACGCGGTCTTCGCCGTCCTCTGCGCCTGCGGCCACAATATCCGTAAGATCCTCGCCCATCTCAGGGCGCTTTGGACCCTATTGC
TTCACCTGATTGCGGCGAGGATTTGGCCCGAAAGGACCCTGCTCAGGGAAGTATTAGCCGCCTGACACGGTTGTTCAGAGCCGAC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1347 bp | 448 aa | 119 | 1465 | + | No |
Chemistry : DDE
ORF sequence :
MKPHSRAPEQDDLLRPRLVDMIDARHELVKLAALIDWEFFEREWATFFPSHQGRPATSPRLVAGLMYLQHAFKLSDEAVVARWVENPYYQHFTGETFFQH
RSPIDPSSLVRWRKRIGEEGVEWLLTKTIEAGQASGAVTDKSLKRVAVDTTVMEKTIAHPTDARLYERARALLAGLAKEAGVDLRQSYARLAPRLAAQVG
RYAHARQFKRMRKALRQLKGYVGRVRRDLRRYLQDIPEGALRGRVLEALWLVGRLLEQTPKSKNKIYALHEPEVDCISKGKARVRYEFGTKVSLATTLDG
GFVVGARSFPGNPYDGHTLASALEQVAILTDQVPALAVVDRGYRGHGVEATKVLISGTRRGITPLLARLLKRRSAIEPEIGHMKSDGRLARCPLKGRIGD
AVFAVLCACGHNIRKILAHLRALWTLLLHLIAARIWPERTLLREVLAA
RSPIDPSSLVRWRKRIGEEGVEWLLTKTIEAGQASGAVTDKSLKRVAVDTTVMEKTIAHPTDARLYERARALLAGLAKEAGVDLRQSYARLAPRLAAQVG
RYAHARQFKRMRKALRQLKGYVGRVRRDLRRYLQDIPEGALRGRVLEALWLVGRLLEQTPKSKNKIYALHEPEVDCISKGKARVRYEFGTKVSLATTLDG
GFVVGARSFPGNPYDGHTLASALEQVAILTDQVPALAVVDRGYRGHGVEATKVLISGTRRGITPLLARLLKRRSAIEPEIGHMKSDGRLARCPLKGRIGD
AVFAVLCACGHNIRKILAHLRALWTLLLHLIAARIWPERTLLREVLAA
Blast result :
Comments
ISPhae1 is 70% aa similar to ISRso9.
ISPhae1 has been identified by its transposition to the entrapment vector pMAT1 (sacB cassette).
As judged from DNA hybridization analysis ISPhae1 is present in 2 copies in P. alcaliphilus JCM 7364. Homologous sequences were also detected by DNA hybridization in P. alcaliphilus JCM 7364; P. aminovorans JCM 7685; P. halophilus JCM 14014; P. yeeii CCUG 32052 and P. yeeii CCUG 32053.
ISPhae1 has been identified by its transposition to the entrapment vector pMAT1 (sacB cassette).
As judged from DNA hybridization analysis ISPhae1 is present in 2 copies in P. alcaliphilus JCM 7364. Homologous sequences were also detected by DNA hybridization in P. alcaliphilus JCM 7364; P. aminovorans JCM 7685; P. halophilus JCM 14014; P. yeeii CCUG 32052 and P. yeeii CCUG 32053.
References
1] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277