ISfinder Mirror Site

ISRsp18

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012586	ND	Rhizobium sp.	Rhizobium sp. NGR234 plasmid pNGR234b

DNA section

IS Length : 1560 bp

Ends

IR Length : 17/19

IRL : GTCGGCCGTGAAGAACTCGGAAGCCGCTGATTAAACATAGGAATCGAGGG
IRR : GTCGATCGTGAAGAACTCGCTTTCAGGCAGTTTGCTGCGGCGTTGGTCGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGACCCTAAA	TC	AATCGGTCGC	2

DNA sequence

GTCGGCCGTGAAGAACTCGGAAGCCGCTGATTAAACATAGGAATCGAGGGATCTGCAGTTCTTCTATTTGCAGGAAACAGCCGCCGAACGGGCTGTTTCC
TTGCAAAATCAGTTTGAGGTTTGCCTGGTTGGCGAAGCGTGATTCACTGGGTTTATCGCGGTGAATCGGAGAGCCAGGAATGGGTAAACCACGCGAACGG
CGCCAAACGGGTGAACAGGATTTATTCCGATCCCGGCTCGACCAGATCATCAATATGAAGCACGAACTGGTGCGTCTGGCGCAGGCTATTGACTGGCCGG
TGTTGGAAGCCCGCTTTGGGACGGTCTATTCGGACGGAGCGGGCATGCCGCCATTGCCGACACGGCTGATGGCGGGGCTGGCCATTCTGAAGCATACGTT
CAACCTGTCGGACGAGGCGCTGTGCGAGCGCTGGGTGGAGAACCCCTATTTTCAGTACCTGTGCGGCGAGGAGTTCTTTCGGCACGAACTATCGTTCGAC
CGCTCCTCGATGACGCGCTGGCGGCAGCGCATGGGCGAGGAACGGATCGGCGCGCTCTTGCAGGAGAGCCTGGCCGTGGCAGTCAAGACAGGCGCGATGA
AGCCGCAGGATACGCGGCAGGTGATCGTCGACACGACCGTTCAACCGAAGAACGTCATGTTCCCGACGGATGCCAAGCTTATTCACCGGGCGCGCGAACG
GCTGGTGCGGCTGGCGAAGAAAATGGGGCTCGACCTGCGCCAGACCTATATTCGGGTCGGTAAATTCGCCCTGATCCAGCACCAGCGCTACGCTCATGCG
AAGCAATTCAAACGGGCCGGCAAGGCGCTGCGCAAGTTGAAGACCTATCTCGGCCGGACCGTTCGCGACATCGAGCGTCAGATCGCCGGCGACGAACAAC
TCGACGCGATCTTCAAATGGTCGCTCTATCAGGCAAAAACGGTCATCGCGCAGCGGCAACGTCAGCGTGGCCGCAAGATCTACAGCCTGCATGCCGACGA
AGTCGAGTGCATTGGTAAAGGCAAGGCGCACAAGCCCTATGAGTTCGGCGTGAAGGTGTCCGTGGCCACAACACTGAACCGTTCAAAGGGCGGGCAGTTC
GCCCTGCACGCGACGGCGCTGCCCGGCAATCCCTACGACGGCCACACGCTCGCGACCGTCATTCCGGATATGGAAAAGATGATCGGCAACGAGCTCTCCC
GCATTCTCGCCGATGCTGGATACCGCGGCCATAATGCTCCCGAAAGTCACAAGCTGAGGGTCTTCACCAGCAGCCAGAAGCGCCGCCTCACGCCCGCCAT
CAAACGCCAGATGCGACGACGATCGGCGGTCGAACCCGTCATTGGACACCTCAAGAGCGAACACAGAATGGACCGGAATTATCTCGCTGGCGAACAGGGG
GATGCCGTCAATGCCGTTCTGGCCGCCGCCGGATACAACTTCTCCCTCCTGCTGCGATGGTTCAGGCAACTTTTGTGTCTGTTCGCCGCACTACTCCTCA
CGTTCCAGAGCCGACCAACGCCGCAGCAAACTGCCTGAAAGCGAGTTCTTCACGATCGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1359 bp	452 aa	180	1538	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGKPRERRQTGEQDLFRSRLDQIINMKHELVRLAQAIDWPVLEARFGTVYSDGAGMPPLPTRLMAGLAILKHTFNLSDEALCERWVENPYFQYLCGEEFF
RHELSFDRSSMTRWRQRMGEERIGALLQESLAVAVKTGAMKPQDTRQVIVDTTVQPKNVMFPTDAKLIHRARERLVRLAKKMGLDLRQTYIRVGKFALIQ
HQRYAHAKQFKRAGKALRKLKTYLGRTVRDIERQIAGDEQLDAIFKWSLYQAKTVIAQRQRQRGRKIYSLHADEVECIGKGKAHKPYEFGVKVSVATTLN
RSKGGQFALHATALPGNPYDGHTLATVIPDMEKMIGNELSRILADAGYRGHNAPESHKLRVFTSSQKRRLTPAIKRQMRRRSAVEPVIGHLKSEHRMDRN
YLAGEQGDAVNAVLAAAGYNFSLLLRWFRQLLCLFAALLLTFQSRPTPQQTA

Blast result :

Comments

ISRsp18 is 90% aa similar to ISRm13.

References

1] ISfinder annotation (2010)
2] Schmeisser,C., Liesegang,H., Krysciak,D., Bakkou,N., Le Quere,A., Wollherr,A., Heinemeyer,I., Morgenstern,B., Pommerening-Roser,A., Flores,M., Palacios,R., Brenner,S., Gottschalk,G., Schmitz,R.A., Broughton,W.J., Perret,X., Strittmatter,A.W. and Streit,W.R. (2009) Appl. Environ. Microbiol. 75 (12), 4035-4045.