ISfinder Mirror Site

ISMisp1

Family IS5
Group IS5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_009654	ND	Marinomonas sp.	Marinomonas sp. MWYL1

DNA section

IS Length : 1497 bp

Ends

IR Length : 12

IRL : GTCGTCCCTGAAAATCTCTCAACCTATTGAATCTGATGTGTTTTTATAAG
IRR : GTCGCCCCTGAATAATGGCGTTTCAGGCAAGAAGCGCCATGCTATTTTTA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CTTATTTAAT	TA	AATAATCTCT	2

DNA sequence

GTCGTCCCTGAAAATCTCTCAACCTATTGAATCTGATGTGTTTTTATAAGATATTTAATACCTAAATCTTACGATAAATACGCCTTTAGACCTAAAAAGT
TGCAGAATGGGTGAGTTTTATGAAACCTCGTCAGACAAAGATCGTCCCGCAAAAGGATTTGTTCCAACCTCAACTCGTAGACATCATTAATCCCAATCAT
CCTTTAATTCGACTGGCTAAGATCATTGATTGGAGGCAATTGGACTCAACGCTTAGTGCCCATTTTTCAAGCGTTGGTGCGGCCGCGTTGCCAACGCGTC
TTATGGCGGGATTGCTGTATTTGCAGCACTTGCATAATTTGTCTGACGAAATGGTATTAGAGCAATGGCTTGAATCGCCTTATTACCAATACTTTTGCGG
CGAAACCTTTTTCCAGCACGACTTCCCGTGCCATCCAACCAGTCTTGTTAAATGGCGAAAACGGTTAGGAGAAGAAGGCTGTGAATGGCTGCTTACCCAG
ACGATACAAGCAGGATTGAAACTAAAAGTCATCAAACCAGCGAGCCTAAAACGAGTCGTGGTAGACACCACGGTACAAGAAAAAAACATCACCTTCCCAA
CCGATGCCAAGCTCTACAACAAGGCTCGACAGCAGCTCACTCAGGTAGCCAAAGAACAAAATATCACGCTAAGACAAACCTACGACAAAGCCTGTCATGA
GCTAATGCCAAAAATTGGACGCTATGGTCACGCCAAACAATACAAACGGATGAGAAAAGCGATTAAGCAAGTGAAAGGCTTTTTAGGGCGAGTCATGCGA
GATATTGATCGGCAAGTAAAACAACAAGGGTTAACGCTCACCCAAAAACAGGAAGACACACTTAACCAAGCCTATCGATTACTAAAACAAACTCGCCAGA
GCAAAAACAAACTGTATAGCTTGCATGAACCTAATGTGGACTGCATATCCAAAGGCAAAGCGCATAAACGCTATGAGTTTGGCGTAAAAGCGAGCATAGC
CGCAACAGCAAAAGAGTCCTTCATCGTGGGAGCCAGAAGCTATCCAGGTAATCCTTATGATGGCCATACCTTGAAAGATCAACTTCAACAAGTTGAAACC
CTAACAGGAAAAAAACCGGAAACCTGTTTTGTTGATAGAGGGTATAAAGGGTCGGGCGTAGACGATATTAAGGTGTTGGTTGCAGGTCAAAAACGAGGTG
TACTCAAGAAAGAGAAGCCATGGATGGGAAGACGAAATAGCGTAGAGCCCATCATAGGGCATCTCAAATCGGATGGAAAACTCAGACGATGCTTCTTAAA
AGGTGTACTAGGCGATGCAATTAACGTGATCTTAAGTGCCTGTGGCCAGAACTTACGTAAGCTACTGAAGTGGCTTTATTACGCCCCATATTTTGGGCCA
TTTTTAAGACGATTATGGTTGAAAATAATTTTTCAACAGGAAGGACCTAAAAATAGCATGGCGCTTCTTGCCTGAAACGCCATTATTCAGGGGCGAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1356 bp	451 aa	120	1475	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKPRQTKIVPQKDLFQPQLVDIINPNHPLIRLAKIIDWRQLDSTLSAHFSSVGAAALPTRLMAGLLYLQHLHNLSDEMVLEQWLESPYYQYFCGETFFQH
DFPCHPTSLVKWRKRLGEEGCEWLLTQTIQAGLKLKVIKPASLKRVVVDTTVQEKNITFPTDAKLYNKARQQLTQVAKEQNITLRQTYDKACHELMPKIG
RYGHAKQYKRMRKAIKQVKGFLGRVMRDIDRQVKQQGLTLTQKQEDTLNQAYRLLKQTRQSKNKLYSLHEPNVDCISKGKAHKRYEFGVKASIAATAKES
FIVGARSYPGNPYDGHTLKDQLQQVETLTGKKPETCFVDRGYKGSGVDDIKVLVAGQKRGVLKKEKPWMGRRNSVEPIIGHLKSDGKLRRCFLKGVLGDA
INVILSACGQNLRKLLKWLYYAPYFGPFLRRLWLKIIFQQEGPKNSMALLA

Blast result :

Comments

ISMisp1 is 65% aa similar to ISRso9.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Johnston,A.W.B., Todd,J.D., Rogers,R., Wexler,M., Bond,P.L., Li,Y. and Richardson,P. (2007) Direct submission GenBank.