ISNpu10
- Family ISAzo13
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010631 | ND | Nostoc punctiforme | Nostoc punctiforme PCC 73102 Nostoc punctiforme PCC 73102 plasmid pNPUN01 |
DNA section
IS Length : 1334 bp
Ends
IR Length : 29/35
IRL : GGCATCATGTAAAAATAACTTGAACAATTTGCTGAATGGTAAGATGATCA
IRR : GGAGTTATGTAAAAATAAATTGAACAATTAACTGACTACTTTGGGTTTGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
ACCGTTTGAGCAATTAG | TAA | GTATTGCCACTGACCTG | 3 |
ATTCATAAAGAAGTTGC | TAA | CAAGGCAGACGAGGGAA | 3 |
GCAAACCCCCTTTACCA | TTG | GCATTGGGACTCAGGGT | 3 |
AGCATAGCTGCCTTTTC | TTA | GTCACATTCAACTATGA | 3 |
AACTCAAGGTGCAACCC | TAA | GAGTTTATCTAGAAAGC | 3 |
TCTGTGTAGCGATCGCT | TAA | CAGTTATTTACCTATTC | 3 |
GCGTAAGACCTATATAC | TTA | ACTATCTTTTAGAAAAA | 3 |
TAAAAGGTGAAGTTCCT | TTA | GCATCTGATTGCAAGTA | 3 |
GCCAGTGCCAAACTCAC | CTA | GTATTGAGATATGCTCT | 3 |
CTTTTTTCCCTCCTTTC | TAA | GGGGGGATTAAGGGGGG | 3 |
GCTACTTCAGCCTGCTG | TAA | CTGAAGTAAAAATGCTT | 3 |
TAGTGTCAGCACGAGTC | TAA | CCCAATAGAACGCTTGC | 3 |
DNA sequence
GGCATCATGTAAAAATAACTTGAACAATTTGCTGAATGGTAAGATGATCAGAATGTAACCGAATTAAAGGCTTATATGTCTGATAAGCAGGTAGTAGAAA
GCATTCAAGACAAGTACGATTCGTTATCGCCTTATTTGAATGAGAAAACACGGCGTATTTGGGCAGCAATTGAAGCCCGAAGCCTGGGCTGGGGAGGCGT
GAGTCAGGTTGCGCTCGCAACTGGACTATCCCGGACTACAATCCATGCTGGGATACGGTTATTGTTAGACGCTTCGGGGGAAAAAACCTCGAATGATGAT
AGTAATCGAATTCGTTCGTCAGGTGCTGGACGTAAACTACTTGAAGAAAAAGACGCAATGCTGCTATCAGATTTAGAATCGCTGATTGAACCAGTGACAC
TGGGAGACCCAGAATCTCCTCTAAAATGGACTTCTAAAAGTGTTGTGAAACTGGCTGCGGCATTAAACATTGGGGGACATAGGACTAGTCCTAAAAGTGT
TTATAACTTACTTGAATCGCTTGGCTACAGCTTACAATCAAATCGTAAAACCCGTGATGGCTCATCTCATCCAGATAGAGATGATCAGTTTTTACATATT
TCCAACCAAGTCTTGCACTTTCAATCCCAGAACGAACCCGTAATTTCAGTTGATACAAAAAAAAAGAATTAATTGGAGATTTTAAAAATTCTGGAACCGA
GTGGTGTGAAAAGGAACAGCCAATTGAGGTGAAAATGCATGATTTTGTTGACCCCAAGTTGGGCAAGGCAATTCCCTACGGAATTTATGACTTAACCTCA
AATCAAGGATGGGTAAATGTTGGCATTGACCACGATACCGCAGAGTTTGCAGTCGAGTCTATTCGTCATTGGTGGTACTCAATGGGTAAACAAGTTTATC
CCAGCAGTGAGCATATAATGATTACGGCTGATTGCGGTGGTAGCAATAGTTATCGCTCACGATTGTGGAAATTGAAGTTACAAGAATTAGCAACTGATAC
TGGTAAAACTATTCATGTGTGTCATTTTCCTCCAGGCACAAGTAAATGGAATAAGATTGAGCATCGCTTGTTTTGTCACATTACCCAAAACTGGCGAGGC
AGACCATTAACTAGCTTGCAAGTTGTGATTAATCTAATTCGCAATACTACCACCACACAAGGATTAGAAGTTGAAGCTAGATTAGATCCAAATCTCTACA
AAACGGGAATCAAGGTTACAGACCAAGAGCTTGATACTATCGCAATCGAACGAAATTCTTTTCATGGTGAGTGGAACTATATTATCAAACCCAAAGTAGT
CAGTTAATTGTTCAATTTATTTTTACATAACTCC
GCATTCAAGACAAGTACGATTCGTTATCGCCTTATTTGAATGAGAAAACACGGCGTATTTGGGCAGCAATTGAAGCCCGAAGCCTGGGCTGGGGAGGCGT
GAGTCAGGTTGCGCTCGCAACTGGACTATCCCGGACTACAATCCATGCTGGGATACGGTTATTGTTAGACGCTTCGGGGGAAAAAACCTCGAATGATGAT
AGTAATCGAATTCGTTCGTCAGGTGCTGGACGTAAACTACTTGAAGAAAAAGACGCAATGCTGCTATCAGATTTAGAATCGCTGATTGAACCAGTGACAC
TGGGAGACCCAGAATCTCCTCTAAAATGGACTTCTAAAAGTGTTGTGAAACTGGCTGCGGCATTAAACATTGGGGGACATAGGACTAGTCCTAAAAGTGT
TTATAACTTACTTGAATCGCTTGGCTACAGCTTACAATCAAATCGTAAAACCCGTGATGGCTCATCTCATCCAGATAGAGATGATCAGTTTTTACATATT
TCCAACCAAGTCTTGCACTTTCAATCCCAGAACGAACCCGTAATTTCAGTTGATACAAAAAAAAAGAATTAATTGGAGATTTTAAAAATTCTGGAACCGA
GTGGTGTGAAAAGGAACAGCCAATTGAGGTGAAAATGCATGATTTTGTTGACCCCAAGTTGGGCAAGGCAATTCCCTACGGAATTTATGACTTAACCTCA
AATCAAGGATGGGTAAATGTTGGCATTGACCACGATACCGCAGAGTTTGCAGTCGAGTCTATTCGTCATTGGTGGTACTCAATGGGTAAACAAGTTTATC
CCAGCAGTGAGCATATAATGATTACGGCTGATTGCGGTGGTAGCAATAGTTATCGCTCACGATTGTGGAAATTGAAGTTACAAGAATTAGCAACTGATAC
TGGTAAAACTATTCATGTGTGTCATTTTCCTCCAGGCACAAGTAAATGGAATAAGATTGAGCATCGCTTGTTTTGTCACATTACCCAAAACTGGCGAGGC
AGACCATTAACTAGCTTGCAAGTTGTGATTAATCTAATTCGCAATACTACCACCACACAAGGATTAGAAGTTGAAGCTAGATTAGATCCAAATCTCTACA
AAACGGGAATCAAGGTTACAGACCAAGAGCTTGATACTATCGCAATCGAACGAAATTCTTTTCATGGTGAGTGGAACTATATTATCAAACCCAAAGTAGT
CAGTTAATTGTTCAATTTATTTTTACATAACTCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
597 bp | 198 aa | 76 | 672 | + | No |
Description : First part of the transposase
ORF sequence :
MSDKQVVESIQDKYDSLSPYLNEKTRRIWAAIEARSLGWGGVSQVALATGLSRTTIHAGIRLLLDASGEKTSNDDSNRIRSSGAGRKLLEEKDAMLLSDL
ESLIEPVTLGDPESPLKWTSKSVVKLAAALNIGGHRTSPKSVYNLLESLGYSLQSNRKTRDGSSHPDRDDQFLHISNQVLHFQSQNEPVISVDTKKKN
ESLIEPVTLGDPESPLKWTSKSVVKLAAALNIGGHRTSPKSVYNLLESLGYSLQSNRKTRDGSSHPDRDDQFLHISNQVLHFQSQNEPVISVDTKKKN
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
654 bp | 217 aa | 654 | 1307 | + | No |
Description : Second part of the transposase
ORF sequence :
YKKKELIGDFKNSGTEWCEKEQPIEVKMHDFVDPKLGKAIPYGIYDLTSNQGWVNVGIDHDTAEFAVESIRHWWYSMGKQVYPSSEHIMITADCGGSNSY
RSRLWKLKLQELATDTGKTIHVCHFPPGTSKWNKIEHRLFCHITQNWRGRPLTSLQVVINLIRNTTTTQGLEVEARLDPNLYKTGIKVTDQELDTIAIER
NSFHGEWNYIIKPKVVS
RSRLWKLKLQELATDTGKTIHVCHFPPGTSKWNKIEHRLFCHITQNWRGRPLTSLQVVINLIRNTTTTQGLEVEARLDPNLYKTGIKVTDQELDTIAIER
NSFHGEWNYIIKPKVVS
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1232 bp | 410 aa | 76 | 1307 | + | Yes |
Chemistry : Unknow
ORF sequence :
MSDKQVVESIQDKYDSLSPYLNEKTRRIWAAIEARSLGWGGVSQVALATGLSRTTIHAGIRLLLDASGEKTSNDDSNRIRSSGAGRKLLEEKDAMLLSDL
ESLIEPVTLGDPESPLKWTSKSVVKLAAALNIGGHRTSPKSVYNLLESLGYSLQSNRKTRDGSSHPDRDDQFLHISNQVLHFQSQNEPVISVDTKKKELI
GDFKNSGTEWCEKEQPIEVKMHDFVDPKLGKAIPYGIYDLTSNQGWVNVGIDHDTAEFAVESIRHWWYSMGKQVYPSSEHIMITADCGGSNSYRSRLWKL
KLQELATDTGKTIHVCHFPPGTSKWNKIEHRLFCHITQNWRGRPLTSLQVVINLIRNTTTTQGLEVEARLDPNLYKTGIKVTDQELDTIAIERNSFHGEW
NYIIKPKVVS
ESLIEPVTLGDPESPLKWTSKSVVKLAAALNIGGHRTSPKSVYNLLESLGYSLQSNRKTRDGSSHPDRDDQFLHISNQVLHFQSQNEPVISVDTKKKELI
GDFKNSGTEWCEKEQPIEVKMHDFVDPKLGKAIPYGIYDLTSNQGWVNVGIDHDTAEFAVESIRHWWYSMGKQVYPSSEHIMITADCGGSNSYRSRLWKL
KLQELATDTGKTIHVCHFPPGTSKWNKIEHRLFCHITQNWRGRPLTSLQVVINLIRNTTTTQGLEVEARLDPNLYKTGIKVTDQELDTIAIERNSFHGEW
NYIIKPKVVS
Blast result :
Comments
ISNpu10 is 65% aa similar to ISArch16. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M. and Atlas,R. (2008) Direct submission GenBank.