ISMtsp5

  • Family IS3
  • Group IS2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010511 ND Methylobacterium sp.
Methylobacterium sp. 4-46
DNA section
IS Length : 1347 bp

Ends


IR Length : 15/26

IRL : TCCTCCGCCCCACGGTTGCTTGAGACACCCCGTTAGCGGACGGTCTCCGC
IRR : TGTACCGCCCGCAGTTTGTGAGACACCGATTTCCAGCCTACGCGGCCAGA

Insertion site


Left flankDirect repeatRight flankDR Length
CGAGAGCAGCAGCGTCTGGACGAC4
TCCTGCACAAGGACCATCTCTTCC4
ATCATGGGTCGCCTATAGGC0

DNA sequence

TCCTCCGCCCCACGGTTGCTTGAGACACCCCGTTAGCGGACGGTCTCCGCGACAGGGGTGAGGACGATGACCACATACACGAGGGCGGGTGCCGATGCGG
TCGGTCCCGGCCGGGGCGGGAGGATGTCCCGGCAGCGCAAGCGGGATGCCGTACTGCGACTGCTGCGCGGTGAGGACCTGGAGACTATCTCGCGCAGCCT
GGGCGTGACCGCCGCGACCCTGTCGGGCTGGCGCGACGCCTTCCTGGCAGCGGGTGAGGCGAGCCTGTCCACCCGGCCCACCGATGCGGAAGCGCTGGAG
AGCGGGCGGCTGAAGGCCAGGCTCGGCGAGATGTTGCTGGAGCGCGAGTTGCTGGAGGCCAAGATCACGGCTCTGGAGGCGCGCGGCCCCGGCCCTTTGG
CCCGCAGGAGGTCGCGGCCATGAGCCAGGTCCTCTCCCCCGTCAGTGGCAAAGCTTACGGGCTGGCCCGCGTCTGCCGGATCTGGGGCCTGTCGCGCGCC
ACCGTCCACCGGCATCTCTCACCCGCGCGGCCGGAGCCGGCGCGGCGTCCCGGCCCGGTTGGGCCGATGCCGGATGCGGCGCTGCTCGACGAGATCCGCG
CCACCCTCACCGGCAGCCCCTTTCACGGCGAGGGTCACCGCAAGGTCTGGGCGCGCCTGCGCCACAAGGGCGTGCGCACCTCCAAGCGCCGCGTGCTGCG
CCTGATGCGCGACCATGATCTGCTCGCCCCCTCGCGCGTCGGCGCGCCGCGCGGCCCACGCACGCACGACGGCACCATCATCCCGGAGGCAGTGGACACG
ATGTGGGGCACCGACCTGACCACCACCTGGACCGGCGAGGGCCAAGTCGCGGTGTTCGTGGCCGTCGACCACTGCTCGGCCGAGTGCGTGGGCGTTCATG
CGGCGCGGCGGGCCACGCGCTTCGAGGCGCTGGAGCCGATCCGCCAGGGTGTGCGGCAGCGCTTCGGCAGCTTCGCGGCCAAGAGTGCTTCCGGCCTGAG
TGTCCGGCACGACCATGGCAGCCAGTACATGTCCGACGCCTTCCAGGCGGAACTGGCGTTCCTGGGCATCGCGAGTTCGCCCGCCTTCGTGCGGGCGCCG
GAGGGCAACGGCTGCGCCGAGCGTTTCATCCGCACGCTCAAGGAGAACCTGCTGTGGGTGGACAGTTTCGACACGGTCGAAGACCTCCGCCGCGCCCTGC
TGGCGTTCCGCGAGATCTACAACGAGACGTGGCTGATCGAGCGGCACGGGTTCCGCCCACCGGCAGCCATTCGTGCCGCACAGCTTTCACCCGCGGCTCT
GGCCGCGTAGGCTGGAAATCGGTGTCTCACAAACTGCGGGCGGTACA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
357 bp118 aa67423+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTTYTRAGADAVGPGRGGRMSRQRKRDAVLRLLRGEDLETISRSLGVTAATLSGWRDAFLAAGEASLSTRPTDAEALESGRLKARLGEMLLERELLEAKI
TALEARGPGPLARRRSRP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1053 bp350 aa2581310+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GEPVHPAHRCGSAGERAAEGQARRDVAGARVAGGQDHGSGGARPRPFGPQEVAAMSQVLSPVSGKAYGLARVCRIWGLSRATVHRHLSPARPEPARRPGP
VGPMPDAALLDEIRATLTGSPFHGEGHRKVWARLRHKGVRTSKRRVLRLMRDHDLLAPSRVGAPRGPRTHDGTIIPEAVDTMWGTDLTTTWTGEGQVAVF
VAVDHCSAECVGVHAARRATRFEALEPIRQGVRQRFGSFAAKSASGLSVRHDHGSQYMSDAFQAELAFLGIASSPAFVRAPEGNGCAERFIRTLKENLLW
VDSFDTVEDLRRALLAFREIYNETWLIERHGFRPPAAIRAAQLSPAALAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1244 bp414 aa671310+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTTYTRAGADAVGPGRGGRMSRQRKRDAVLRLLRGEDLETISRSLGVTAATLSGWRDAFLAAGEASLSTRPTDAEALESGRLKARLGEMLLERELLEAKI
TALEARGPGPFGPQEVAAMSQVLSPVSGKAYGLARVCRIWGLSRATVHRHLSPARPEPARRPGPVGPMPDAALLDEIRATLTGSPFHGEGHRKVWARLRH
KGVRTSKRRVLRLMRDHDLLAPSRVGAPRGPRTHDGTIIPEAVDTMWGTDLTTTWTGEGQVAVFVAVDHCSAECVGVHAARRATRFEALEPIRQGVRQRF
GSFAAKSASGLSVRHDHGSQYMSDAFQAELAFLGIASSPAFVRAPEGNGCAERFIRTLKENLLWVDSFDTVEDLRRALLAFREIYNETWLIERHGFRPPA
AIRAAQLSPAALAA

 

Blast result :
Comments
ISMtsp5 is 43% aa similar to ISButh1.
References
1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M.,Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2008) Direct submission GenBank.