ISBlo15
- Family IS200/IS605
- Group IS1341
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AB495342 | ND | Bifidobacterium longum | Bifidobacterium longum BK28 |
DNA section
IS Length : 1593 bp
Ends
Left end : CAGTTTCCGGGCGGGGATGCCCGGTATTTCAATGCCGGGAGGAACCACCCTCTCCTTTCCTTTTAAGTCTTTTTCATTATACGCGCGTAGTGATATAATT II struct. : No
Right end : CCGCCTATCGCGGGAAGGACGCTTCCGCCATGACGTGCGAAACCTCAACACGATAACAACGTGTTGGAATCCCGGTGTTTCAACACCGGGAGGACGTCAA II struct. : Yes
Insertion site
| Left flank | LE cleavage site | Right flank | RE cleavage site |
|---|---|---|---|
| AAGCCCGGACGGGCTTTCAC | ATACCATCACGCAGCACTCG | tcaa |
DNA sequence
CAGTTTCCGGGCGGGGATGCCCGGTATTTCAATGCCGGGAGGAACCACCCTCTCCTTTCCTTTTAAGTCTTTTTCATTATACGCGCGTAGTGATATAATT
GTAATCATGTCCCAGAAGGTCGTGATAGAACGTGTTCTGATGCGGGGAGCCGCCCCATATCTCGGCATGTGTGCGACCGAGGACGGACAGCAAGTCCCCC
TGTATTCGACCAATCCCGACGTAGTGATGCGTTGGCTGTGCGACGGGTGGCGTTGCCGATACAACCAGTTGCGTTCCCGTCGCACCAAGTGGGACAGGGA
ATCCCAGACGGCCGTCCCGTTGAACGGGAGGCCGGACATGCGTTCCGACCGTCAGGCGCGATTGGAATGCTCATGGCTTACGGCCATGCCCGCGATGGTT
TTGCAATCCCCGAACCGGATAGAGAACACGGACTGGTGGAGCGCGAACAAGCGCCGCAGAACGTTGAAGAAAAAGCATCGGAATCCGGGCATGATGCCCC
GGTTCAAATCCCGTCATGACGACCTGTATTTCGTCTGCTGGCACAATAAGGGGTCGAACGCGAACTACCGGCGGTTGAACCGGCATCACGGCGAGGTCGT
GATAACCGGCCAGAACCCCGGCGAATATCGTCTGGACGGCCAGCCGTGCCGGTACAGCATCCACATCCGCGTCCGCGTCAGCCAGCCCATCCGCGACTAT
ACGAGCATCGGGGTGAACTGGACACGACGCACGCTCGTGTTCGTCAACGACCCGCTGCCGATAGGCCGTGAGCGCACGGGTGCCATGGTCGGCTTAGACA
GGGGTTGCGCGCACACACTTGCCACGTCCGACAATCGTTTCCTCGACCTTCCCAAGCAGCGGTTGGAGCGTATCGACCGTGAAATACGCCGCCGTCAGAA
GGCTCAGGCGCGACGGGTCAACATGTCGGGCAAGACGGTGAGGGAATACCGTCGCAATCCGAGCCGCACCTACGAGCGTACCGGTCGGGAAATCAGCCGA
CTGTACGCGAAGGCCCACAGAATCATAGACGACTGGCAGCACAAGACCACCAGCATGCTCGTCCATGACTACGACCTCATCGGGCTGGAAGACCTCAACC
TGCAAGGCATGAGTCGCAAAGCCAAGGCGAAACCCGACCCAGACAGGCCGGGCGCGTACCTGCATAACGGCCAGTCGGCGAAACACGGCCTCAACCATGC
CCTACGCTCCGCCGGTCTCGCCGGTATAGTCGAAAAACTCGAATACAAGACCCGGCTGACCGGACAGAACCGGCTTATACTCGTCAACCCCGCGTACACG
TCGCAGACATGCAGTGAATGCGGTTATTGCGACAGTCGAAACCGCGAAAGCCAAGCGGACTTCGAGTGCAAGCAATGCCACATGAGCATGAACGCCGACT
TGAACGCGGCCAACAACATCCTCAAACGGGGATTGAACCATCTCATCGGCTTGGACGAAGCCGAGCACGCCCGAACGGAGCCAGACGCCCAACCCGCCTA
TCGCGGGAAGGACGCTTCCGCCATGACGTGCGAAACCTCAACACGATAACAACGTGTTGGAATCCCGGTGTTTCAACACCGGGAGGACGTCAA
GTAATCATGTCCCAGAAGGTCGTGATAGAACGTGTTCTGATGCGGGGAGCCGCCCCATATCTCGGCATGTGTGCGACCGAGGACGGACAGCAAGTCCCCC
TGTATTCGACCAATCCCGACGTAGTGATGCGTTGGCTGTGCGACGGGTGGCGTTGCCGATACAACCAGTTGCGTTCCCGTCGCACCAAGTGGGACAGGGA
ATCCCAGACGGCCGTCCCGTTGAACGGGAGGCCGGACATGCGTTCCGACCGTCAGGCGCGATTGGAATGCTCATGGCTTACGGCCATGCCCGCGATGGTT
TTGCAATCCCCGAACCGGATAGAGAACACGGACTGGTGGAGCGCGAACAAGCGCCGCAGAACGTTGAAGAAAAAGCATCGGAATCCGGGCATGATGCCCC
GGTTCAAATCCCGTCATGACGACCTGTATTTCGTCTGCTGGCACAATAAGGGGTCGAACGCGAACTACCGGCGGTTGAACCGGCATCACGGCGAGGTCGT
GATAACCGGCCAGAACCCCGGCGAATATCGTCTGGACGGCCAGCCGTGCCGGTACAGCATCCACATCCGCGTCCGCGTCAGCCAGCCCATCCGCGACTAT
ACGAGCATCGGGGTGAACTGGACACGACGCACGCTCGTGTTCGTCAACGACCCGCTGCCGATAGGCCGTGAGCGCACGGGTGCCATGGTCGGCTTAGACA
GGGGTTGCGCGCACACACTTGCCACGTCCGACAATCGTTTCCTCGACCTTCCCAAGCAGCGGTTGGAGCGTATCGACCGTGAAATACGCCGCCGTCAGAA
GGCTCAGGCGCGACGGGTCAACATGTCGGGCAAGACGGTGAGGGAATACCGTCGCAATCCGAGCCGCACCTACGAGCGTACCGGTCGGGAAATCAGCCGA
CTGTACGCGAAGGCCCACAGAATCATAGACGACTGGCAGCACAAGACCACCAGCATGCTCGTCCATGACTACGACCTCATCGGGCTGGAAGACCTCAACC
TGCAAGGCATGAGTCGCAAAGCCAAGGCGAAACCCGACCCAGACAGGCCGGGCGCGTACCTGCATAACGGCCAGTCGGCGAAACACGGCCTCAACCATGC
CCTACGCTCCGCCGGTCTCGCCGGTATAGTCGAAAAACTCGAATACAAGACCCGGCTGACCGGACAGAACCGGCTTATACTCGTCAACCCCGCGTACACG
TCGCAGACATGCAGTGAATGCGGTTATTGCGACAGTCGAAACCGCGAAAGCCAAGCGGACTTCGAGTGCAAGCAATGCCACATGAGCATGAACGCCGACT
TGAACGCGGCCAACAACATCCTCAAACGGGGATTGAACCATCTCATCGGCTTGGACGAAGCCGAGCACGCCCGAACGGAGCCAGACGCCCAACCCGCCTA
TCGCGGGAAGGACGCTTCCGCCATGACGTGCGAAACCTCAACACGATAACAACGTGTTGGAATCCCGGTGTTTCAACACCGGGAGGACGTCAA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1410 bp | 469 aa | 140 | 1549 | + | No |
AG : TnpB
ORF sequence :
MRGAAPYLGMCATEDGQQVPLYSTNPDVVMRWLCDGWRCRYNQLRSRRTKWDRESQTAVPLNGRPDMRSDRQARLECSWLTAMPAMVLQSPNRIENTDWW
SANKRRRTLKKKHRNPGMMPRFKSRHDDLYFVCWHNKGSNANYRRLNRHHGEVVITGQNPGEYRLDGQPCRYSIHIRVRVSQPIRDYTSIGVNWTRRTLV
FVNDPLPIGRERTGAMVGLDRGCAHTLATSDNRFLDLPKQRLERIDREIRRRQKAQARRVNMSGKTVREYRRNPSRTYERTGREISRLYAKAHRIIDDWQ
HKTTSMLVHDYDLIGLEDLNLQGMSRKAKAKPDPDRPGAYLHNGQSAKHGLNHALRSAGLAGIVEKLEYKTRLTGQNRLILVNPAYTSQTCSECGYCDSR
NRESQADFECKQCHMSMNADLNAANNILKRGLNHLIGLDEAEHARTEPDAQPAYRGKDASAMTCETSTR
SANKRRRTLKKKHRNPGMMPRFKSRHDDLYFVCWHNKGSNANYRRLNRHHGEVVITGQNPGEYRLDGQPCRYSIHIRVRVSQPIRDYTSIGVNWTRRTLV
FVNDPLPIGRERTGAMVGLDRGCAHTLATSDNRFLDLPKQRLERIDREIRRRQKAQARRVNMSGKTVREYRRNPSRTYERTGREISRLYAKAHRIIDDWQ
HKTTSMLVHDYDLIGLEDLNLQGMSRKAKAKPDPDRPGAYLHNGQSAKHGLNHALRSAGLAGIVEKLEYKTRLTGQNRLILVNPAYTSQTCSECGYCDSR
NRESQADFECKQCHMSMNADLNAANNILKRGLNHLIGLDEAEHARTEPDAQPAYRGKDASAMTCETSTR
Blast result :
Comments
References
1] Fukiya, S., Sugiyama, T., Kano, Y., and Yokota, A. (2009) in preparation. FEMS Microbiology letters; Identification, cloning and characterization of novel insertion sequence ISBlo15 inserted into a cryptic plasmid in Bifidobacterium longum BK28.