ISRhru5

  • Family IS5
  • Group IS427
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007643 ND Rhodospirillum rubrum
Rhodospirillum rubrum ATCC 11170
DNA section
IS Length : 842 bp

Ends


IR Length : 14/21

IRL : GCGGTGAACTCATAAATCTGCTTTCCTCTCGCCGTGGTCCGTGATTCCCG
IRR : GCCGTCGACTCATTATGGCTGACACCAAATCCGCAGGGCGACGAGTTTAA

Insertion site


Left flankDirect repeatRight flankDR Length
GGTAAGGAAGCTAGATGAAGCGCC4

DNA sequence

GCGGTGAACTCATAAATCTGCTTTCCTCTCGCCGTGGTCCGTGATTCCCGTCCTGGAGCGGGGGTTTTGATGACGCGTCGGCGCTATGAATTGACGGAAT
ATGAGTGGTCGGTGATCCAGCCTTTGTTGCCGAACAAGCAGCGCGGAGTTCCCCGCGTCGATGACCGGCGGGTCCTCAATGGAATTCTGTGGCGCTTTCG
TACCGGGTCGCCGTGGGCAGAAGTCCCCGAGCGCTACGGCCCGCCGACTACCTGCTACAACCGCTTCGTCCGCTGGCGCAAGGCCGGGGTGTGGGACCGG
CTTTCGGAAGAAGTTTCAAAGACCTACGATGGCGACATCGTCATGATCGACTCGACCTGTGTCCGTGTTCACCAGCACGGAGCCACGGGAAAAAAGGGGA
TCTTGAGGATGGCGGCATGGGACGTTCCCGTGGCGGCCTCATCAGCAAAATCCACGCGCTCGTCGATGCGGAAGGCCGCCCCGTCACCTTGCGGCTGACC
GGCGGCCAAGTTCATGATAGCTTGGAAGCCGAGGCGTTGATCGAAAACCTCGGCGAGGGTGACATCCTGCTCGCCGACAAGGGCTATGACAGCAACGCCA
TTCGCGCCCTGGCCGCAGAGAAGAAGGCTTGGGCCAATATCCCGCCCCGCTCCAACCGCAAAGGCAGCTTCGCCTTTTCTTCCTGGGTCTACCGCCAATG
CACTCTGGTCGAGCGCTTCTTCAACAGGATCAAGCAGTTCCGGGGCATCGCCACCCGATATGACAAAGACCCGCTCAATTTCCTCGCTGCCGTTAAACTC
GTCGCCCTGCGGATTTGGTGTCAGCCATAATGAGTCGACGGC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
429 bp142 aa70498+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTRRRYELTEYEWSVIQPLLPNKQRGVPRVDDRRVLNGILWRFRTGSPWAEVPERYGPPTTCYNRFVRWRKAGVWDRLSEEVSKTYDGDIVMIDSTCVRV
HQHGATGKKGILRMAAWDVPVAASSAKSTRSSMRKAAPSPCG

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
666 bp221 aa165830+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PAGPQWNSVALSYRVAVGRSPRALRPADYLLQPLRPLAQGRGVGPAFGRSFKDLRWRHRHDRLDLCPCSPARSHGKKGDLEDGGMGRSRGGLISKIHALV
DAEGRPVTLRLTGGQVHDSLEAEALIENLGEGDILLADKGYDSNAIRALAAEKKAWANIPPRSNRKGSFAFSSWVYRQCTLVERFFNRIKQFRGIATRYD
KDPLNFLAAVKLVALRIWCQP

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
761 bp253 aa70830+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTRRRYELTEYEWSVIQPLLPNKQRGVPRVDDRRVLNGILWRFRTGSPWAEVPERYGPPTTCYNRFVRWRKAGVWDRLSEEVSKTYDGDIVMIDSTCVRV
HQHGATGKKGDLEDGGMGRSRGGLISKIHALVDAEGRPVTLRLTGGQVHDSLEAEALIENLGEGDILLADKGYDSNAIRALAAEKKAWANIPPRSNRKGS
FAFSSWVYRQCTLVERFFNRIKQFRGIATRYDKDPLNFLAAVKLVALRIWCQP

 

Blast result :
Comments
ISRhru5 is 87% aa similar to IS869. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Munk,A.C., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Mavrommatis,K., Richardson,P., Zhang,Y., Roberts,G., Reslewic,S., Zhou,S. and Schwartz,D.C. (2009) Direct submission GenBank.