ISMamg2

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_007626 ND Magnetospirillum magneticum
Magnetospirillum magneticum AMB-1
DNA section
IS Length : 1486 bp

Ends


IR Length : 12/17

IRL : TGGAAGGCCAAGTATGGCGGGCTCGACGTGTCGGAAGCCCGGCGGCTGAA
IRR : TGGAAGCCTCAGAATCGTCATAAGCTCTCCGGGGGTAATGAGGTGATCCA

Insertion site


Left flankDirect repeatRight flankDR Length
CATCCCATTAAGCCTTAAATCG2

DNA sequence

TGGAAGGCCAAGTATGGCGGGCTCGACGTGTCGGAAGCCCGGCGGCTGAAAGCGCTTGAGGACGAGAACGCCCAGCTGAAGAAGCTGCTGGCGGAATCCA
TGCTGAACGAGGCCGCGCTCCGCGATCTGCTGGGAAAAAAATGGTGAGGCCCGCCGCCAAGCGAGAAGCTGTCGCCCATCTGCGAAGCCAATTCGAGATG
AGCGAGCGTCGGGCCTGTGCGGTGATTGCCGCCGACCGTATGACGATCCGCTACCGGTCGCGCCGCCCCAGGGACGAGGCGCTGCGTGCCCGCCTGCGGG
ACTTGGCCAACCTCCGGCGGCGTTTCGGCTATCGTCGGCTGTTCATCCTGCTGCACGAGACGGGGGAGCCATCGGGACTGAGCCGTATCTATCGGCTTTA
TCGCGAGGAGGGCTTGGCGGTGCGCAAGCGTAAGACCCGGCGCAAGGCCATTGGCAGCCGTGCCCCGATCCTGGTCGAGGCCCGCGCCAACGCCCGCTGG
TCGCTGGACTTCGTCCATGACCAATTGGCCTGTGGACGGCGGTTCCGCATCCTCAACGTGGTCGACGACGTGACGCGGGAGTGCCTGGCCGCCATTCCCG
ACACCTCGATCTCGGGGGCCAGGGTGACCCGTGAACTGTCGGCGCTGATTGCTCGGCGAGGGCGACCAGAGATGATCGTCTCGGACAACGGCACCGAGCT
GACGTCCAATGCCGTCCTGGCCTGGAAACAGCAGCAGCGGATCGACTGGCATTACATCGCGCCGGGAAAGCCCATGCAGAACGGCTTCGTCGAAAGCTTC
AATGGCCGCATGCGCGACGAGTTGCTCAACGAGACGGTCTTCACCAGCCTGCCCCAGGCCCGGGCCGTCATCGCCGCCTGGGCGGACGACTACAACACCG
CCAGACCACACTCATCCCTGGGATACCAGACCCCGGCGGCGCACGCCGCCAAACTCAAGGCAATGGGGCCATCCTCTCCGCCCCTCCGGGGCTCCGAGCC
CGGCCCCATTGCCCACACCGCGCCAGACGGCATAACTTTGCCAAAGGCTCTACTTTTCGCCGGAGGAAAGTTCCCGAGCAGGTCAGCTCGACGGCGAGAT
TTTCACCACCATGCCCACGACGAACCCGTCCGGAATCGCCATCAAAATCGACATCCTATGCCCATCTGGCGACAGGATGTCGATTTTCGGATCTTCATCA
CCATGCTGACACCAAGTGTGAAGCGATCTCCCAGATCAAAATTCCATTGTGTACGCGAGGCGACGACCAACTCACTGGTAACAGGTTTCGATTCCGCATT
AATTAGCGCCGGGTTAGCCCATAAATCTGCCAATTCACAAACAACGTTCGATCTGTTTGCAGAAAAAAATAGACCCCCACTTCACTTTTTATAGTGACTT
TCGCTACCATGATTCAATGCAACATGGAGCACGACATGGATCACCTCATTACCCCCGGAGAGCTTATGACGATTCTGAGGCTTCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
117 bp38 aa31147+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSEARRLKALEDENAQLKKLLAESMLNEAALRDLLGKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1335 bp444 aa601394+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GRERPAEEAAGGIHAERGRAPRSAGKKMVRPAAKREAVAHLRSQFEMSERRACAVIAADRMTIRYRSRRPRDEALRARLRDLANLRRRFGYRRLFILLHE
TGEPSGLSRIYRLYREEGLAVRKRKTRRKAIGSRAPILVEARANARWSLDFVHDQLACGRRFRILNVVDDVTRECLAAIPDTSISGARVTRELSALIARR
GRPEMIVSDNGTELTSNAVLAWKQQQRIDWHYIAPGKPMQNGFVESFNGRMRDELLNETVFTSLPQARAVIAAWADDYNTARPHSSLGYQTPAAHAAKLK
AMGPSSPPLRGSEPGPIAHTAPDGITLPKALLFAGGKFPSRSARRRDFHHHAHDEPVRNRHQNRHPMPIWRQDVDFRIFITMLTPSVKRSPRSKFHCVRE
ATTNSLVTGFDSALISAGLAHKSANSQTTFDLFAEKNRPPLHFL

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1364 bp455 aa311394+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSEARRLKALEDENAQLKKLLAESMLNEAALRDLLGKKMVRPAAKREAVAHLRSQFEMSERRACAVIAADRMTIRYRSRRPRDEALRARLRDLANLRRRF
GYRRLFILLHETGEPSGLSRIYRLYREEGLAVRKRKTRRKAIGSRAPILVEARANARWSLDFVHDQLACGRRFRILNVVDDVTRECLAAIPDTSISGARV
TRELSALIARRGRPEMIVSDNGTELTSNAVLAWKQQQRIDWHYIAPGKPMQNGFVESFNGRMRDELLNETVFTSLPQARAVIAAWADDYNTARPHSSLGY
QTPAAHAAKLKAMGPSSPPLRGSEPGPIAHTAPDGITLPKALLFAGGKFPSRSARRRDFHHHAHDEPVRNRHQNRHPMPIWRQDVDFRIFITMLTPSVKR
SPRSKFHCVREATTNSLVTGFDSALISAGLAHKSANSQTTFDLFAEKNRPPLHFL

 

Blast result :
Comments
ISMamg2 is 81% aa similar to IS511. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Matsunaga,T., Okamura,Y., Fukuda,Y., Wahyudi,A.T., Murase,Y. and Takeyama,H. (2005) DNA Res. 12 (3), 157-166.