ISMno14
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011892 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 Methylobacterium nodulans ORS 2060 plasmid pMNOD01 |
DNA section
IS Length : 1428 bp
Ends
IR Length : 9/12
IRL : tcacacaTGGGGTAATCGCGGGAGCGTTCCCCCTCGGGCCGGAGTGACGC
IRR : ---gctgTCGTCTAATCGCCTCGCATAAAGCAAGTCCTTTTTGGATTGTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGCGAAATGCCGTTT | CGGTCGGAAGCCGCC | 0 | |
| GGGCGTCGGTCGTTT | CGGTGGACAGCAGCT | 0 | |
| GATGAAATGCGGTTT | CCACCCGAGCCGGAA | 0 | |
| ACAAAACCCGCGTTC | GGCACGCGAGGCGAT | 0 |
DNA sequence
TCACACATGGGGTAATCGCGGGAGCGTTCCCCCTCGGGCCGGAGTGACGCGGCCTACAATGGAAGCGGTGGTTGTTCCCCGGCAGGATGAAGTTGCGGGC
TCACGACTGGACGCCGGGCCCATGCATCGAGCGGAGAACAACCATGGGTGAGTATATCGGTCTCGACGTGTCCCTGAAAGAGACGGCGCTCAGCGTGCGG
CGGGACGGCAAGCGTGTTTGGCGCGGCAGGTGCGCTTCCGATCCTGCCGTCATTGCCAGGGTGCTGCGCAAGCATGCGCCCGAAGCCCAACGGATCGTAT
TTGAGACGGGTCCTTTGTCAGTTTGGTTCTACCACGCGCTGCGCGCCGAAGGCTTGCCAGCGATCTGCATCGACGCGCGCCATGCCAAGGCCGCGCTCGA
CATGGCCGCCAACAAAACCGACGCCAACGATGCCGACGGGCTGGCCCATCTGGCCGAGGTCGGCTTCTTCCGCGAAGTGCGGGTGAAGGGATACGACAGC
CTGCTCGTGCGAACGCTTGTCGCCGCGCGCAGCAAGCTCGTGCAGGTGACGACCGAGCTATCCAATCAGATCCGCGGCCTGATGAAGACGTTCGGCTTGA
TCGTGCCGGGCGCAAAGGGCGGATCGTTCGAGGCGGAGGTTCGACGTCTCCTGTCGGGCGATCACGGTCTGGCTCGCGTCATCCTTCCGCTGCTTGAGGC
CTGGAACGCGGTTCGCCGTCGAGCAGCGGAGCTCGGACGCCAGCTTCTGGCCGGTGCACGGCAGAGCCAAGCCTGCCAGCGGCTGATGGCGATTCCCGGG
ATCGGGGCGATCACCGCGACCGCCTTCGTGACCGCGATCGAGGATCCCGCCAACTTCCGATCGTCCCGCTCCGTCGCCGCCTGGTTCGGGTTGACGACGC
GGCGCTATCAGTCGGGCGAGGTCGACTATGACGGGCATATCTCCCGGAGAGGAGACGCCCATATGCGAGGGCTTCTCTACGAGGCGGCGACCGTTCTCCT
GACGCGTAGCCAGGTGGACAGCGACCTGCGAGCCTGGGGACTGAAGCTGCGCGAGCGGCTCGGCTTCAAGCGCGCCGCCGTGGCGGTCGCGCGCAAGCTG
GCCGTCGTCATGCACGCCATGCTGAAGTCCGGCCACTCCTACCGTGCGAAGGCTGCCATGGCCTGATCCCAGAACACCCCAGCTTGTCGCGTCCCGAAGG
CGCGTCGAGGCGTCCCTGCCGGGACGTGGGCGAACCATTCCGCCTAGGCTGTTGCACCGTGAGATCCAGACCGGTCCATCGAGTGCGCTTCACACATCGG
AAGGTTCATCCTGCGAAGCCCCATCATGCGGCGGCTCATGCCGACCGCGAAGACGACCGTGCTCCCGGCAAACGACGGCCCGACAATCCAAAAAGGACTT
GCTTTATGCGAGGCGATTAGACGACAGC
TCACGACTGGACGCCGGGCCCATGCATCGAGCGGAGAACAACCATGGGTGAGTATATCGGTCTCGACGTGTCCCTGAAAGAGACGGCGCTCAGCGTGCGG
CGGGACGGCAAGCGTGTTTGGCGCGGCAGGTGCGCTTCCGATCCTGCCGTCATTGCCAGGGTGCTGCGCAAGCATGCGCCCGAAGCCCAACGGATCGTAT
TTGAGACGGGTCCTTTGTCAGTTTGGTTCTACCACGCGCTGCGCGCCGAAGGCTTGCCAGCGATCTGCATCGACGCGCGCCATGCCAAGGCCGCGCTCGA
CATGGCCGCCAACAAAACCGACGCCAACGATGCCGACGGGCTGGCCCATCTGGCCGAGGTCGGCTTCTTCCGCGAAGTGCGGGTGAAGGGATACGACAGC
CTGCTCGTGCGAACGCTTGTCGCCGCGCGCAGCAAGCTCGTGCAGGTGACGACCGAGCTATCCAATCAGATCCGCGGCCTGATGAAGACGTTCGGCTTGA
TCGTGCCGGGCGCAAAGGGCGGATCGTTCGAGGCGGAGGTTCGACGTCTCCTGTCGGGCGATCACGGTCTGGCTCGCGTCATCCTTCCGCTGCTTGAGGC
CTGGAACGCGGTTCGCCGTCGAGCAGCGGAGCTCGGACGCCAGCTTCTGGCCGGTGCACGGCAGAGCCAAGCCTGCCAGCGGCTGATGGCGATTCCCGGG
ATCGGGGCGATCACCGCGACCGCCTTCGTGACCGCGATCGAGGATCCCGCCAACTTCCGATCGTCCCGCTCCGTCGCCGCCTGGTTCGGGTTGACGACGC
GGCGCTATCAGTCGGGCGAGGTCGACTATGACGGGCATATCTCCCGGAGAGGAGACGCCCATATGCGAGGGCTTCTCTACGAGGCGGCGACCGTTCTCCT
GACGCGTAGCCAGGTGGACAGCGACCTGCGAGCCTGGGGACTGAAGCTGCGCGAGCGGCTCGGCTTCAAGCGCGCCGCCGTGGCGGTCGCGCGCAAGCTG
GCCGTCGTCATGCACGCCATGCTGAAGTCCGGCCACTCCTACCGTGCGAAGGCTGCCATGGCCTGATCCCAGAACACCCCAGCTTGTCGCGTCCCGAAGG
CGCGTCGAGGCGTCCCTGCCGGGACGTGGGCGAACCATTCCGCCTAGGCTGTTGCACCGTGAGATCCAGACCGGTCCATCGAGTGCGCTTCACACATCGG
AAGGTTCATCCTGCGAAGCCCCATCATGCGGCGGCTCATGCCGACCGCGAAGACGACCGTGCTCCCGGCAAACGACGGCCCGACAATCCAAAAAGGACTT
GCTTTATGCGAGGCGATTAGACGACAGC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 144 | 1166 | + | No |
Chemistry : DEDD
ORF sequence :
MGEYIGLDVSLKETALSVRRDGKRVWRGRCASDPAVIARVLRKHAPEAQRIVFETGPLSVWFYHALRAEGLPAICIDARHAKAALDMAANKTDANDADGL
AHLAEVGFFREVRVKGYDSLLVRTLVAARSKLVQVTTELSNQIRGLMKTFGLIVPGAKGGSFEAEVRRLLSGDHGLARVILPLLEAWNAVRRRAAELGRQ
LLAGARQSQACQRLMAIPGIGAITATAFVTAIEDPANFRSSRSVAAWFGLTTRRYQSGEVDYDGHISRRGDAHMRGLLYEAATVLLTRSQVDSDLRAWGL
KLRERLGFKRAAVAVARKLAVVMHAMLKSGHSYRAKAAMA
AHLAEVGFFREVRVKGYDSLLVRTLVAARSKLVQVTTELSNQIRGLMKTFGLIVPGAKGGSFEAEVRRLLSGDHGLARVILPLLEAWNAVRRRAAELGRQ
LLAGARQSQACQRLMAIPGIGAITATAFVTAIEDPANFRSSRSVAAWFGLTTRRYQSGEVDYDGHISRRGDAHMRGLLYEAATVLLTRSQVDSDLRAWGL
KLRERLGFKRAAVAVARKLAVVMHAMLKSGHSYRAKAAMA
Blast result :
Comments
ISMno14 is 66% aa similar to ISMdi10.
References
1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.