ISfinder Mirror Site

ISMtsp18

Family IS3
Group IS2

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010511	ND	Methylobacterium sp.	Methylobacterium sp. 4-46

DNA section

IS Length : 1341 bp

Ends

IR Length : 27/33

IRL : TGAACTGCACCCCGTTTTGACCGGACAGGCGGCATAGCCCAGGAAGGCAT
IRR : TGGAGTGCACCCAGTTTGACCGGACAGGTAGCGGGGTAGGTTTAGGCACT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATTTCAAGCA	CTTAG	CGGGCTCGAA	5

DNA sequence

TGAACTGCACCCCGTTTTGACCGGACAGGCGGCATAGCCCAGGAAGGCATAGGCGCAGGCCTATGGATAAGGCTATGGCATCTGATCCGATCGCCCCGCC
TCCGCACGGCGAGATCCTGACCGGTGTGCAGCGCCGCCGGCGGTGGTCCACGGCCGAGAAGATCCGCCTCGTCGAGGAGAGCCAGCAGCCCGGCTCGTCC
GTGTCCTTCGTGGCGCGGCGCTACGGGCTGTCGCCCAGCCTGCTGTTCCGGTGGAAGCGGCGCTTGCTGGAAGGCGGGCATGCGGCTGTTCAGGCCGATG
AGGAGGTCGTCGGCACCAGCCGGGTGCGCGAGCTGGAGCGGCGCGTGCGGGACTTGGAACGCCTGCTCGGGCGCAAGACCATGGAGGTCGAGATCCTCAA
GGAGGCGCTCGACCTCGCACGCACAAAAAAACCGACCTTGCCGCTCGTCTCCTGGGGCGGGCCGGTGGGCGGTTCCCGATGAAGGCGGTCGCCGACACGC
TGGGCGTGGCTCGCTCCCATCTCGCCGAGCGCCTCACCCGCCCCGTCCGGCCGCGTGGTCCCTCCCGCAGGCCCCAGGACGCCGTGTTGCTGCCCACCAT
CCGCGAGATCGTCGCGGCCCGTCCCAGCTACGGCTACCGGCGTATCACCGCGCTGCTGAACAGGGTGCTGCGCTCGCAAGGCGCGTCGGTCGTCAACGCC
AAGCGGGTGCTGCGCATCCTCCAGGTGAACGGGCTGACGCTGGCGCCCTATACCGCTCGCCGCACCGGCCGGACCCATGACGGTGTCGTGGTCGCGCTGC
GCTCGAACGTGCGCTGGTGCTCGGACCATCTCGACCTGCGCTGCCGGGACGGAGCGGTGGTCCGGGTGCTGTTTGCCATCGACGCCTGCGACCGTGAGAT
CATGGCTTGGTCGGCGACCACGGCCGGCGTCTCCGGCGAGATGGTCAGCAACCTGATGATCGCCTGCGTCGAGCACCGCTTCGGCGCGACGAAGGCCCCG
CACCCGATCGAGTGGCTGTCCGACAACGGCTCGGCTTACATCGCCCGCCAGACGAGCGAGACGGCCACCGCGCTCGGGCTGCGGCTGCTGTTCACACCGG
TGCGCTCCCCGCAGAGCAACGGCATTGCCGAAGCCTTCGTGAAAACGCTCAAGCGTGACTACGCACGCCTTGCGATGCTGCCCGACGCCGAGACCGTGAT
GCGGCTTCTGCCGAGTTGGTTCGAGGACTACAACACCGTCCACCCGCACTCCGGTTTGCGTATGCTCTCGCCTCGCGAGTTCATCAGCCAGAGTGCCTAA
ACCTACCCCGCTACCTGTCCGGTCAAACTGGGTGCACTCCA

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
420 bp	139 aa	63	482	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MDKAMASDPIAPPPHGEILTGVQRRRRWSTAEKIRLVEESQQPGSSVSFVARRYGLSPSLLFRWKRRLLEGGHAAVQADEEVVGTSRVRELERRVRDLER
LLGRKTMEVEILKEALDLARTKKPTLPLVSWGGPVGGSR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
999 bp	332 aa	302	1300	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GGRRHQPGARAGAARAGLGTPARAQDHGGRDPQGGARPRTHKKTDLAARLLGRAGGRFPMKAVADTLGVARSHLAERLTRPVRPRGPSRRPQDAVLLPTI
REIVAARPSYGYRRITALLNRVLRSQGASVVNAKRVLRILQVNGLTLAPYTARRTGRTHDGVVVALRSNVRWCSDHLDLRCRDGAVVRVLFAIDACDREI
MAWSATTAGVSGEMVSNLMIACVEHRFGATKAPHPIEWLSDNGSAYIARQTSETATALGLRLLFTPVRSPQSNGIAEAFVKTLKRDYARLAMLPDAETVM
RLLPSWFEDYNTVHPHSGLRMLSPREFISQSA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1238 bp	412 aa	63	1300	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDKAMASDPIAPPPHGEILTGVQRRRRWSTAEKIRLVEESQQPGSSVSFVARRYGLSPSLLFRWKRRLLEGGHAAVQADEEVVGTSRVRELERRVRDLER
LLGRKTMEVEILKEALDLARTKKTDLAARLLGRAGGRFPMKAVADTLGVARSHLAERLTRPVRPRGPSRRPQDAVLLPTIREIVAARPSYGYRRITALLN
RVLRSQGASVVNAKRVLRILQVNGLTLAPYTARRTGRTHDGVVVALRSNVRWCSDHLDLRCRDGAVVRVLFAIDACDREIMAWSATTAGVSGEMVSNLMI
ACVEHRFGATKAPHPIEWLSDNGSAYIARQTSETATALGLRLLFTPVRSPQSNGIAEAFVKTLKRDYARLAMLPDAETVMRLLPSWFEDYNTVHPHSGLR
MLSPREFISQSA

Blast result :

Comments

ISMtsp18 is 75% aa similar to IS426.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M.,Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2008) Direct submission GenBank.