ISMpo8
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_010725 | ND | Methylobacterium populi | Methylobacterium populi BJ001 |
DNA section
IS Length : 849 bp
Ends
IR Length : 12/16
IRL : GGTCAAAACCCTATCAAGCTATTGATCTGTCGACGTAATGTGGCTTCCAT
IRR : GGTCGAGACTCAATCAGCACCAGAAGGCAATGACGGCGGCAAGGGCGAGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTCCGGCTG | CTAG | CCCGTCTTAT | 4 |
DNA sequence
GGTCAAAACCCTATCAAGCTATTGATCTGTCGACGTAATGTGGCTTCCATGGTGCGCCTTGAGACAGGGAGGCGCGCCATGGCGGACTTGTTCTGGCTGT
CGGACGAGCAGTGGGTGGTCATCGAACCGTTCATGCCCAAGAACCAGCCCGGGCCCGAACGCAACGACGACCGGCAGGTCATCTCCGGCATCATCCACGT
CGTGAAGACGGGCTGTCGCTGGTGCGATTGCCCGTCGGAGTACGGTCCGCCCACCACGATCTACAACCGCTTCAATCGCTGGTCCCGGCGCGGCTTCTGG
AAGGCGATGCTGGTCGCCCTGGCCAAGGCCGGATGGTCCGGCGAGGCCAACGCTATCGACGCCACCTACGTCAAAGCGCACCGCTCCGCCCAGGGCGGAA
AAGGGGGGCCAAGGCGCAAGACATCGGTCCCTCGCGCGGCGGCCAGACGACCAAGATCCACGCGCTCGTCGATGTCGTGGGCCGCCCGGCGGTCCTCCTT
CTGACACCCGGCAATGCCAGCGACGTGAGGACCGCACCGGATGTGCTGGCCGAGGCCCCCGGCCGCATCCGCCGCCTGATTGCCGACAAGGGCTATGACG
CCAACTGGCTGCGAAAAGACCTGCGCGCGAGCAACACCACACCGATCATCCCCGGCACCCGCGCGCGCAAGCGCAAGATCAGGCTCGACAAGAAACGCTA
CCGCGAGCGCTGGCGGGTAGAGGCGACCTTCTGCCGACTGAAGGACTTCCGTAGGATTGCCACACGCTACGACAAACTCGCCCGAAACTTCGCCTCAGCA
CTCGCCCTTGCCGCCGTCATTGCCTTCTGGTGCTGATTGAGTCTCGACC
CGGACGAGCAGTGGGTGGTCATCGAACCGTTCATGCCCAAGAACCAGCCCGGGCCCGAACGCAACGACGACCGGCAGGTCATCTCCGGCATCATCCACGT
CGTGAAGACGGGCTGTCGCTGGTGCGATTGCCCGTCGGAGTACGGTCCGCCCACCACGATCTACAACCGCTTCAATCGCTGGTCCCGGCGCGGCTTCTGG
AAGGCGATGCTGGTCGCCCTGGCCAAGGCCGGATGGTCCGGCGAGGCCAACGCTATCGACGCCACCTACGTCAAAGCGCACCGCTCCGCCCAGGGCGGAA
AAGGGGGGCCAAGGCGCAAGACATCGGTCCCTCGCGCGGCGGCCAGACGACCAAGATCCACGCGCTCGTCGATGTCGTGGGCCGCCCGGCGGTCCTCCTT
CTGACACCCGGCAATGCCAGCGACGTGAGGACCGCACCGGATGTGCTGGCCGAGGCCCCCGGCCGCATCCGCCGCCTGATTGCCGACAAGGGCTATGACG
CCAACTGGCTGCGAAAAGACCTGCGCGCGAGCAACACCACACCGATCATCCCCGGCACCCGCGCGCGCAAGCGCAAGATCAGGCTCGACAAGAAACGCTA
CCGCGAGCGCTGGCGGGTAGAGGCGACCTTCTGCCGACTGAAGGACTTCCGTAGGATTGCCACACGCTACGACAAACTCGCCCGAAACTTCGCCTCAGCA
CTCGCCCTTGCCGCCGTCATTGCCTTCTGGTGCTGATTGAGTCTCGACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 426 bp | 141 aa | 79 | 504 | + | No |
Description : First part of the transposase
ORF sequence :
MADLFWLSDEQWVVIEPFMPKNQPGPERNDDRQVISGIIHVVKTGCRWCDCPSEYGPPTTIYNRFNRWSRRGFWKAMLVALAKAGWSGEANAIDATYVKA
HRSAQGGKGGPRRKTSVPRAAARRPRSTRSSMSWAARRSSF
HRSAQGGKGGPRRKTSVPRAAARRPRSTRSSMSWAARRSSF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 534 bp | 177 aa | 303 | 836 | + | No |
Description : Second part of the transposase
ORF sequence :
GDAGRPGQGRMVRRGQRYRRHLRQSAPLRPGRKRGAKAQDIGPSRGGQTTKIHALVDVVGRPAVLLLTPGNASDVRTAPDVLAEAPGRIRRLIADKGYDA
NWLRKDLRASNTTPIIPGTRARKRKIRLDKKRYRERWRVEATFCRLKDFRRIATRYDKLARNFASALALAAVIAFWC
NWLRKDLRASNTTPIIPGTRARKRKIRLDKKRYRERWRVEATFCRLKDFRRIATRYDKLARNFASALALAAVIAFWC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 758 bp | 252 aa | 79 | 836 | + | Yes |
Chemistry : DDE
ORF sequence :
MADLFWLSDEQWVVIEPFMPKNQPGPERNDDRQVISGIIHVVKTGCRWCDCPSEYGPPTTIYNRFNRWSRRGFWKAMLVALAKAGWSGEANAIDATYVKA
HRSAQGGKGGAKAQDIGPSRGGQTTKIHALVDVVGRPAVLLLTPGNASDVRTAPDVLAEAPGRIRRLIADKGYDANWLRKDLRASNTTPIIPGTRARKRK
IRLDKKRYRERWRVEATFCRLKDFRRIATRYDKLARNFASALALAAVIAFWC
HRSAQGGKGGAKAQDIGPSRGGQTTKIHALVDVVGRPAVLLLTPGNASDVRTAPDVLAEAPGRIRRLIADKGYDANWLRKDLRASNTTPIIPGTRARKRK
IRLDKKRYRERWRVEATFCRLKDFRRIATRYDKLARNFASALALAAVIAFWC
Blast result :
Comments
ISMpo8 is 64% aa similar to ISPam4.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C. and Richardson,P. (2008) Direct submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C. and Richardson,P. (2008) Direct submission GenBank.