ISfinder Mirror Site

ISMtsp13

Family IS256
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010511	ND	Methylobacterium sp.	Methylobacterium sp. 4-46 Methylobacterium sp. 4-46 plasmid pM44601

DNA section

IS Length : 1428 bp

Ends

IR Length : 23/27

IRL : GGTCGTGTCCCTGGTGGTGGTGTAGCTCGACGGGAGCGAGAGAGCCCGCC
IRR : GGTCGTGTCCCCAGGCGTGGTGTAGCTGCGGCGTGGCCACCACAGTCACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGGCGGCAGA	CCTGCGGG	CGCCCTGGTT	8
ATCGTCCATC	GAGGCCCG	GGCGCGCGTC	8
CGCGAGCTCG		GACATCCGAC	0

DNA sequence

GGTCGTGTCCCTGGTGGTGGTGTAGCTCGACGGGAGCGAGAGAGCCCGCCTGCGCGCCCTCCACAGCGCCGAAGCAGGCGGGATCTCTCGCGGGGTGGTC
ATCGGCGGTTGCCGGGTAGGGTCGGGGTGCGAGCACCAACCTCGAACCCGAGAGACCTCCGATGACCGACGAGATGATGAGCCTGCGCGGGCTGCTGGAG
AAGAGCGCCGACGCCGACCTGCTGCGCGAGATGATCGGCTTTGCGGCCGAGCGGCTGATGGAACTGGAGGTGGGCGGCCTGACCGGGGCGGCCCACGGCG
AGAAGAGCGCCGAGCGGCTGGTCCAGCGCAACGGCTATCGTGACCGGGATTGGCAGACGCGGGCCGGCACGGTCGAGCTGCGCATCCCCAAGCTGCGCAA
GGGCTCGTACTTCCCGGCTTTCCTGGAGCCGCGCCGGATGGCCGAGAAGGCACTCACGGCGGTGATCCAGGAGGCCTACATCCAGGGCATCTCGACCCGC
TCGGTGGATGACCTCGTCCAAGCCATGGGCGGCACGGGCGTGTCCAAGAGCCAGGTCAGCCGGCTCTGCCAGGAGATCGACGAGCGCGTGGGCGCGTTCC
TCGACCGGCCGCTCGAGGGCGAGTGGTCCTATCTCTGGATCGATGCGACCTCCGTGAAGGTGCGCCAAGCCGGGCGCATCGTCTCCGTCGCCGTGATCGT
GGCGGTCGGTGTGAACAGCGACGGCCGCAGGGAAGCGCTCGGCATGGACATTGGCCCCTCCGAGGCTGAGACGTTCTGGACCGGGTTCCTGCGCAAGCTG
GCGCGCCGCGGCCTGCGCGGTGTGAAGCTGGTGATTTCGGACGCCCACACGGGCCTCAAGGCGTCGGTCGCCAAGGTGATGAACGCGACTTGGCAGCGGT
GCCGCGTCCACTTCATGAGGACCGTGCTCGCCCACGCTGGCCGCAGCGGGCGGCGCGTCGTGTCCGCCTTCATCGCCACCGCCTTCGCCCAGGACGACGC
GGAGGCCGCCCGTCAGCAATGGCGACGCGTGGCCGACCAACTCCGACCCAAGGTGCCCAAGCTCGCAGCCCTGATGCATGAGGCGGAACCCGACGTGCTG
GCCTACATGGGCTTCCCGGCCGCCCACCGGGTCAAGCTTCACAGCACGAACCCGCTGGAGCGCCTCAACGGCGAGATCAAGCGGCGCACCGAGGTGGTCG
GCATCTTCCCCAATGAGGCGGCCATCACCCGCTTGGTCGGCGCGCTCCTGCTCGAACAGAACGACGAGTGGGCCGTCCAGCGCGCCCGCTACCTGACCCT
GGAAAGCATCGCCCCGATCAGCGATGATCCTCTTGTCAGCCTGCCCACCCTGGCAGCCTGATCGTCCCGGCCCCGCCCGGTGACTGTGGTGGCCACGCCG
CAGCTACACCACGCCTGGGGACACGACC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1200 bp	399 aa	162	1361	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTDEMMSLRGLLEKSADADLLREMIGFAAERLMELEVGGLTGAAHGEKSAERLVQRNGYRDRDWQTRAGTVELRIPKLRKGSYFPAFLEPRRMAEKALTA
VIQEAYIQGISTRSVDDLVQAMGGTGVSKSQVSRLCQEIDERVGAFLDRPLEGEWSYLWIDATSVKVRQAGRIVSVAVIVAVGVNSDGRREALGMDIGPS
EAETFWTGFLRKLARRGLRGVKLVISDAHTGLKASVAKVMNATWQRCRVHFMRTVLAHAGRSGRRVVSAFIATAFAQDDAEAARQQWRRVADQLRPKVPK
LAALMHEAEPDVLAYMGFPAAHRVKLHSTNPLERLNGEIKRRTEVVGIFPNEAAITRLVGALLLEQNDEWAVQRARYLTLESIAPISDDPLVSLPTLAA

Blast result :

Comments

ISMtsp13 is 89% aa similar to ISAli6.

References

1] Ming-Chun Lee and David Robinson (2009) Direct submission.

2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M.,Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2008) Direct submission GenBank.