ISfinder Mirror Site

ISMtsp8

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010511	ND	Methylobacterium sp.	Methylobacterium sp. 4-46 Methylobacterium sp. 4-46 plasmid pM44601

DNA section

IS Length : 2371 bp

Ends

IR Length : 36/50

IRL : TGTGAGCGGGCGTTGGATCCTCCTCACTTGTGGGCCTGCAATTTTCCCCA
IRR : TGTCAACGGGCTTCGAACCTTCCGCAATTTCGGGCGCGCAATTTTCCTCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ACGACGAGGA	CCTTCG	TCGGCGAGGT	6
GCCGCGGGTG	GGCGGG	TTCCGGTCGT	6
GGCCGATGCC	GACGCC	GGGCCGGGCC	6
CTAGCCTGCA	CCGAT	CACAGCGTGA	5
AGGCGTAGGT	CTGCG	TACTGGTCGT	5
GCTGGACCAT	GGACG	CGTCGAACCC	5
GCCCTTGTCC	ATCAG	CGCGTCCAGC	5
CCTGAGCAGG		CGAATACGCT	0
GCCGAATACG		GCAGGTTCAA	0

DNA sequence

TGTGAGCGGGCGTTGGATCCTCCTCACTTGTGGGCCTGCAATTTTCCCCACCCTCCCGGCCGCCCCTGAGCCTCCTGTTTGAGGCGGGATGCCCGCGCGG
CGGCCGTCTCACCGAAGCTGGTCCCCGTGGCGGGAGGGAGCGGCGCGGTGGTCAGCCTCGGGGAACTCATGACGATCCTGGACCTCCACCGGCAGGGCCT
CTCGGTCACCGCCATCGCCCGCCAGCTCGGCCTCGACCGCAAGACCGTCGCCAAGTACATCGCCCGCGGCCTCGAGCCGCCCGTCTACGGACCGCGATCC
CCCCGCGCGCGGGCCACCGACGCCTTCCTGCCCTACCTGCGCGAGCGCCTGGCCGCCTACCCGCAGCTTACGGCCGTCCGTCTCTGGCGCGAGTTGAAGG
AGCGCGGCTTCGCAGGGGCCTACACCGCCGTGAAGCGAGCCGTGGCCCTGCTTCGCCCCTCAGCTCCTCTGCCTATCGAGCGCCGCTTCGAGACCCCGCC
GGGCGAGCAGGCCCAGGTCGACCTCGCCCGCTTCGAGGTCGTCTTCGCCGACGAGCCGGGCGTGACCCGCATCGTCTGGCTGTTCGCGATGGTGCTGGGC
CACTCGCGCTATCTCTGGGCCCGCTTCGTCGTCCACCAGGATCTGCAGACGGTCCTGCGCTGCCACATCGCCGCCTTCCAGGCCCTTGGAGGCGCCCCGC
GCGAGATCCTCTACGACCGCATGAAGACCGCCGTGATCGGCGAGGATCCCGACGGCTTGGTCATCTATAACCGCAGCCTTCTCGATCTCGCGCGCCACTA
CGGGTTCCTGCCGCGCGCCTGCCGTCCCTACCGGGCCAAGACCAAGGGCAAGGTCGAGCGCCCGTTCCGCTACCTGCGCGAGGACTTCTTCCTCGCCCGC
TCGTTCCGCAACCTCGACGACCTGAACGACCAGCTGCGGCACTGGCTCGACACCGTGGCCAACGCCCGCCGGCACGCCACGACCAAGCGGATCGTCGCCG
ACGCCTTCGCGGAGGAGCGCAGCCAGCTGCGGGCGCTGCCGCCCGTGCCCTACGAAGCCGTGCTCAGCCTGGAGCGGCGCGTCACCCACGAGGGCTTCGT
CTCGGTGGCGGGCAATCTCTACAGCGTGCCCGACACCACCCGCCGCCGCGCCCTGGAGGTGCACGTGCTGGCCGATCAGATCCGCATCTACGAGGCGGGT
GAGCTCGTTGCCTGCCACCTGCCCCTGGAAGGGCGTGGGCTGACGCAGGTCGATCCAGCCCATCGGCGGCCGCGATCTCCCCCGCCCGAGCCACGAGACC
CTGCCGAGCCGGTGGTCGTCAGGCGCGCCGGCGACCAGGTCGCGCGTCGCCCGCTGGCCATCTACGACGCCGTAGCCCGCCAACTCGCGGGAGCCGGCGT
GCCCAGGACCGACCGGGGAGACGCGGCATGAGCGGAACTGGCGAGCTGATCCCCCCACTGGTCGAGCGGATCAAGGCCACGCTGGTAGGGCTGAAGATGC
CGCGCGCCCTGGAGATCGTCGACACCACCGTGCGGCGGCTGGAGCGCGGCGAACTCAGCGCGCTGGAGGCGGTCGATGCCCTGCTGAGCGAGGAGCTGAG
CCTACGCGAGAGCCGGCGGGTGAAGACCGCGCTGGTGATGGCGCGGCTCTCGACGGTCAAGACGCTGTCGGGCTTCGACTTCGCCTTCCAGCCCTCGCTC
GACCGCACCCGCATCCTGGCCCTGGCCGAGCTGGGCTTCGTGGACCGCTGCGAGGTGCTGCACTTCCTCGGCCCGCCCGGCACCGGCAAGAGCCACTTGG
CGGTGGCCCTCGGGGTCGAGGCGGTGAAGGCGGGCCGCAGCGTGTACTTCACCACCCTGGCCGACCTTGTGGGAACGCTGGCGCGGGCCGAGCGGGAAGG
AACGTTGCGCGAGAAGATCCGCTACTTCTGCCGGCCGGCGCTGCTGATCGTGGACGAGATCGGCTACCTGCCGGTGGTGCCGGGCGGGGGCAACCTGTTC
TTCCAGCTCGTCAACGCGCGCTACGAGCGGGGCGCGATGGTCCTGACCTCGAACCGCGGCTTTGCAGAGTGGGGGGAGGTGTTCGGCGATCCGGTGGTGG
CGACCGCGCTGCTGGACCGGTTGCTTCACCACGCCGTGGTGGTGCAGATCGAGGGCTCAAGCTACCGGCTGCGCCAGCACACCGCGCTCATGCCCGAGCA
CATCCGCTCGAAGGCAGCCCTGCAGGCTCCGCCGCTCGCCCCGCCTCCGCGTCGGCGCGGACGCCCGCCCAAGAATGGAGGTGCTCACCTCGGCATCGCC
TGACCGCCGAGCCCGCCGATCTGAGGAAAATTGCGCGCCCGAAATTGCGGAAGGTTCGAAGCCCGTTGACA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1284 bp	427 aa	148	1431	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

VVSLGELMTILDLHRQGLSVTAIARQLGLDRKTVAKYIARGLEPPVYGPRSPRARATDAFLPYLRERLAAYPQLTAVRLWRELKERGFAGAYTAVKRAVA
LLRPSAPLPIERRFETPPGEQAQVDLARFEVVFADEPGVTRIVWLFAMVLGHSRYLWARFVVHQDLQTVLRCHIAAFQALGGAPREILYDRMKTAVIGED
PDGLVIYNRSLLDLARHYGFLPRACRPYRAKTKGKVERPFRYLREDFFLARSFRNLDDLNDQLRHWLDTVANARRHATTKRIVADAFAEERSQLRALPPV
PYEAVLSLERRVTHEGFVSVAGNLYSVPDTTRRRALEVHVLADQIRIYEAGELVACHLPLEGRGLTQVDPAHRRPRSPPPEPRDPAEPVVVRRAGDQVAR
RPLAIYDAVARQLAGAGVPRTDRGDAA

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
876 bp	291 aa	1428	2303	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSGTGELIPPLVERIKATLVGLKMPRALEIVDTTVRRLERGELSALEAVDALLSEELSLRESRRVKTALVMARLSTVKTLSGFDFAFQPSLDRTRILALA
ELGFVDRCEVLHFLGPPGTGKSHLAVALGVEAVKAGRSVYFTTLADLVGTLARAEREGTLREKIRYFCRPALLIVDEIGYLPVVPGGGNLFFQLVNARYE
RGAMVLTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVVQIEGSSYRLRQHTALMPEHIRSKAALQAPPLAPPPRRRGRPPKNGGAHLGIA

Blast result :

Comments

ISMtsp8 is 76%(orfA), and 88%(orfB) aa similar to ISMex13.

References

1] Ming-Chun Lee and David Robinson, direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T.,Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M.,Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2008) Direct submission GenBank.