ISMno28
- Family IS6
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011892 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 plasmid pMNOD01 |
DNA section
IS Length : 892 bp
Ends
IR Length : 18/21
IRL : GGGCCTGTCGCAAACGCGGATGGGCCCACGTTCACCGGTCGTTAGCCACA
IRR : GGGCTTGTCGCAAACTCGGGTTCAGGCCGCAGGAGGGCCTGCTCGGCCCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGCCGTATCC | GCATCGAGCA | 0 |
DNA sequence
GGGCCTGTCGCAAACGCGGATGGGCCCACGTTCACCGGTCGTTAGCCACAGCCGCCCAGCTTGCCGCCTCCCTCCGGAGCCGGCCCATGATCCTCCACGC
CCTCGTCCTCAAGCTGAAGCGACAGGCCCGCGGTGACTTCAGGGGCCGGCACTTCGAAGCCACGCTGATCGTCCAGGTCGTCTCCTGGTACTTGCGCTAC
GCGCTCAGCTATCGCGATATCGAGGAGATGCTGCTGGAACGCGGCCTGGAGGTCGACCACTCCACCATCAATCGCTGGGTGCTCGCCTATGCCCCCGCCA
TCGAGCGCCGGCTGCGCATGTTTCGCAAGCCGCACTGCGGCTCGGTGCGCGTCGACGAAACGTACATCAAGATCCGCGGCCAGTGGCGCTATCTGTACCG
GGCCATCGACAAGCACGGCGAAGCGGTCGACTTCCTCCTCACCGCGAACCGCGATCTTGACGCCGCCAAGCGCTTCTTCCGCAAGATGCTGCGGGAGGAA
CCGCTGCTCGCCCCGGACCGGATCGGCACGGACGGCGCTGGTCCTTATCCGCCGGCCCTCGCCGAGAGCCGGCAGGACGGTCTGCTGCCGCGCACGCCCA
CCCACCACGTCACCAAGCACCTGCAGCAGGGGATCGAGAGCGACCACTTCCGGGTGAAGAGGCCGATGCCGCGGGTGGGCGGGTTCCCTCCTTCACCACG
GCCCGGCGGACGATCCAGGGCTTCGAGGCCATGCTGTGGCTGCGCAAGGGCTTCGGGTTTGCGGGGGCCTGGACCGTGCGGGAGCAGAACCAGCTGCTGG
CGTACTGCTTCGGACTTCCCGTCGCGAACAAAGCGTGAAAGCGGGGCCGAGCAGGCCCTCCTGCGGCCTGAACCCGAGTTTGCGACAAGCCC
CCTCGTCCTCAAGCTGAAGCGACAGGCCCGCGGTGACTTCAGGGGCCGGCACTTCGAAGCCACGCTGATCGTCCAGGTCGTCTCCTGGTACTTGCGCTAC
GCGCTCAGCTATCGCGATATCGAGGAGATGCTGCTGGAACGCGGCCTGGAGGTCGACCACTCCACCATCAATCGCTGGGTGCTCGCCTATGCCCCCGCCA
TCGAGCGCCGGCTGCGCATGTTTCGCAAGCCGCACTGCGGCTCGGTGCGCGTCGACGAAACGTACATCAAGATCCGCGGCCAGTGGCGCTATCTGTACCG
GGCCATCGACAAGCACGGCGAAGCGGTCGACTTCCTCCTCACCGCGAACCGCGATCTTGACGCCGCCAAGCGCTTCTTCCGCAAGATGCTGCGGGAGGAA
CCGCTGCTCGCCCCGGACCGGATCGGCACGGACGGCGCTGGTCCTTATCCGCCGGCCCTCGCCGAGAGCCGGCAGGACGGTCTGCTGCCGCGCACGCCCA
CCCACCACGTCACCAAGCACCTGCAGCAGGGGATCGAGAGCGACCACTTCCGGGTGAAGAGGCCGATGCCGCGGGTGGGCGGGTTCCCTCCTTCACCACG
GCCCGGCGGACGATCCAGGGCTTCGAGGCCATGCTGTGGCTGCGCAAGGGCTTCGGGTTTGCGGGGGCCTGGACCGTGCGGGAGCAGAACCAGCTGCTGG
CGTACTGCTTCGGACTTCCCGTCGCGAACAAAGCGTGAAAGCGGGGCCGAGCAGGCCCTCCTGCGGCCTGAACCCGAGTTTGCGACAAGCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 752 bp | 250 aa | 87 | 838 | + | No |
Chemistry : DDE
ORF sequence :
MILHALVLKLKRQARGDFRGRHFEATLIVQVVSWYLRYALSYRDIEEMLLERGLEVDHSTINRWVLAYAPAIERRLRMFRKPHCGSVRVDETYIKIRGQW
RYLYRAIDKHGEAVDFLLTANRDLDAAKRFFRKMLREEPLLAPDRIGTDGAGPYPPALAESRQDGLLPRTPTHHVTKHLQQGIESDHFRVKRPMPRVGGF
PSFTTARRTIQGFEAMLWLRKGFGFAGAWTVREQNQLLAYCFGLPVANKA
RYLYRAIDKHGEAVDFLLTANRDLDAAKRFFRKMLREEPLLAPDRIGTDGAGPYPPALAESRQDGLLPRTPTHHVTKHLQQGIESDHFRVKRPMPRVGGF
PSFTTARRTIQGFEAMLWLRKGFGFAGAWTVREQNQLLAYCFGLPVANKA
Blast result :
Comments
ISMno28 is 96% aa similar to ISMtsp1.
The transposase was reconstructed in silico by joining (87-686 ; 686-838), there is a ponctual mutation inducing a frameshift.
The transposase was reconstructed in silico by joining (87-686 ; 686-838), there is a ponctual mutation inducing a frameshift.
References
1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.