ISfinder Mirror Site

ISCde1

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010424	ND	Candidatus Desulforudis	Candidatus Desulforudis audaxviator MP104C

DNA section

IS Length : 2422 bp

Ends

IR Length : 21/24

IRL : GTAAGCGTCAAATAGCCCCCACCTAGCTTTTCGCCCTCCCAAATGAGATA
IRR : GTAAGCGTCAAATACTTCCCACCTTTAAAGCCAGGTGAGGATTGATTTAG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGTCGAGTAC		GATGGTACTA	0
GGCGAGGAAT		CTATGGAGTG	0

DNA sequence

GTAAGCGTCAAATAGCCCCCACCTAGCTTTTCGCCCTCCCAAATGAGATACTCTACGCAGCAAAAATCGAAGGAGGGCATCGCCACGTGACCAAAGCAGA
GCTACAGGGCTTACGAAACCAATGGGCCACCAGAGTAGCCGAGTTTCAGGCCAGCGGGCAGAGCGTGTCCGCCTGGTGCGCGGAACAGCAGGTAAAACCC
CATCAACTAAGATACTGGCTCAAGAAACATTCCCAAGCCGTCGGAAATGCACCGACCCAGTGGTTGTCACTGAACTTAAGAGACCTTGCTAATCCACCCC
TGGTCATCAGAATCGGCCAAGCCACCGTCGAAGTGCGGCCGGGCTTTGATCCCGATCTACTTCGTGATGTGGTGCGCACCCTGGCACAATGATCAGAGAA
ACCGAACACCACCGGGTCTATCTGGCTTGCGGCAGCACCGACCTGCGCAAATCCATCGACGGGTTGGCCGTATTGGTCAAGGAAGGATTCCGCCTGGATC
CGTTCTCCTCGTGCCTTTTCGTGTTCTGCAACCGCAAACGGGACAAACTCAAGATCCTCCAGTGGGAGCATAACGGTTTCTGGCTGCACTATCGTCGGCT
CGAGCGCGGCAAATTCCAGTGGCCGACCAAAGATAATTCTGGAACAGTCGCACCGGTCACCTACCGGGAGTTGCGTTGGCTGCTTGACGGTCTGTCACTT
GCGCAGCCAGCCCATTCGGAGGTCAGGGCCAGAACGATTTTGTAATAAAGAAAATGATGTTTAAGCCTTGATTTTAAAGGGTTTTTCGTGCCATGTGTCG
AATTGGTAGACATGGATAATGCAGCGAAAACCATCGAAGAACTCCAGATAAAATGTGCTTTACAGCAACAGCAAATCGCTGAACTAACGGCCAAACTTAA
CTGGTTTGAAGAACAGTTCCGTCTCAGCCAACAGCGTCAATTCGGCCGCTCCAGTGAGCAGACCCAAAACCAAGTGGAGCTTTTCAACGAGGCAGAGGCC
GAAGCCAGAGCGTCTTTCGAACCAACGATCGAGGAAATCACCTACCGCCGCCGCAAAAAGCAGGGCCGGCGCCAGGAACAGCTAAAGGATCTGCCGGAGG
AGATCATTGAATACCGGCTCGCTCCGGAAGAGCAGAGATGTGCCTGCGGCGGCGCCCTGCACGAAATGAGCACCGAGGTCAGGCAGGAACTCAAAATCAT
TCCGGCCCAGGTCAGTGTCGTCAAGCACGTCCGCTATGTCTACGCCTGCCGCCGCTGTGAACGGGAAGACATCAAAACCCCCATCGTCACCGCCCCGATG
CCGGCGGCGGTACTGCCGGGAAGCCTGGTTTCCCCCTCGGCCATGGCCTACATTATGACCCAAAAGTACGTGGAGGGCATGCCGCTTTACCGCCAGGAAC
AACACCTGGCCCGTCGGGGCGTGGAACTCTCCCGCCAAACCCTGGCCAACTGGATGATCCAGGGTGCGGATCGCTGGCTAAGCCTCCTGTATGCCCGGAT
GCACAAGCATCTACTGGCGCAAGACATCCTGCACGCCGATGAGACGACCTTGCAGGTACTCAATGAACCGGGCCGGTCGGCGCAAAGCACTTCCTACCTC
TGGCTTTACCGCACCGGGCGGGCCGGACCGCCAATAATCCTTTATGACTACCAGACCACCCGGGCCAGTAAACATCCCCGCCGGTTCTTGTCCGGCTTTA
AGGGTTACCTGCATGTCGACGGCTATACCGGCTACAACGAACTGCCGGATGTGACCCTGGTCGGGTGTTGGGCGCATGCCCGGCGCAAGTTCGACGAAGC
GCTAAAAGCACTGCCCAACGCCCAGCGCGGTGCGGCGGTGGCCGCCAAAGAAGGGTTGGAGTTCTGCAACCGTCTCTTTGCCATCGAACGGGAGTTCCGT
GAAGTCACTCCCCAGGAGCGTCATACGCGTCGCCAGGAACTCAGTCGGCCGGTGGTGGAGGCTTTTTCAGCCTGGCTGAAATACCAGAGCCCCAGAGTTC
TGCCGAAAAGCGCCTTCGGCCAAGCCATCAAGTATTGCCACAACCAGTGGGACAGGCTTACCGTCTTTCTGGAAGACGGCCGCCTGGAGTTGGACAACAA
CCGCAGTGAGCGCTCCATTAAACCATTTGTCATCGGCCGTAAGAACTGGCTATTCGCGAACACCGCCCGTGGGGCAAGCGCCAGTGCCATCATTTATAGT
GTTGTGGAAACAGCGAAGGAAAACGGCCTCAACCCCTTCAGTTACCTGCAGTATCTTTTTGTAAAGCTGCCGAACATGGATATTCAGGATGAACAGGCCT
TAGAAGAGTTGCTTCCCTGGTCGGCAACACTGCCACCGATCTGTCGGGGTGGCAAGTAGTCTATTAGAGAGTCTAAATCAATCCTCACCTGGCTTTAAAG
GTGGGAAGTATTTGACGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
306 bp	101 aa	87	392	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

VTKAELQGLRNQWATRVAEFQASGQSVSAWCAEQQVKPHQLRYWLKKHSQAVGNAPTQWLSLNLRDLANPPLVIRIGQATVEVRPGFDPDLLRDVVRTLA
Q

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
357 bp	118 aa	389	745	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MIRETEHHRVYLACGSTDLRKSIDGLAVLVKEGFRLDPFSSCLFVFCNRKRDKLKILQWEHNGFWLHYRRLERGKFQWPTKDNSGTVAPVTYRELRWLLD
GLSLAQPAHSEVRARTIL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1572 bp	523 aa	788	2359	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPCVELVDMDNAAKTIEELQIKCALQQQQIAELTAKLNWFEEQFRLSQQRQFGRSSEQTQNQVELFNEAEAEARASFEPTIEEITYRRRKKQGRRQEQLK
DLPEEIIEYRLAPEEQRCACGGALHEMSTEVRQELKIIPAQVSVVKHVRYVYACRRCEREDIKTPIVTAPMPAAVLPGSLVSPSAMAYIMTQKYVEGMPL
YRQEQHLARRGVELSRQTLANWMIQGADRWLSLLYARMHKHLLAQDILHADETTLQVLNEPGRSAQSTSYLWLYRTGRAGPPIILYDYQTTRASKHPRRF
LSGFKGYLHVDGYTGYNELPDVTLVGCWAHARRKFDEALKALPNAQRGAAVAAKEGLEFCNRLFAIEREFREVTPQERHTRRQELSRPVVEAFSAWLKYQ
SPRVLPKSAFGQAIKYCHNQWDRLTVFLEDGRLELDNNRSERSIKPFVIGRKNWLFANTARGASASAIIYSVVETAKENGLNPFSYLQYLFVKLPNMDIQ
DEQALEELLPWSATLPPICRGGK

Blast result :

Comments

ISCde1 is 64%(orfA), 78%(orfB), and 79%(orfC) aa similar to ISSwo2.

References

1] ISfinder annotation (2009).
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Pitluck,S., Lowry,S.R., Larimer,F., Land,M.L., Hauser,L., Kyrpides,N., Ivanova,N.N. and Richardson,P. (2007) Direct submission GenBank.