ISCsc1
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Clostridium scindens | Clostridium scindens ATCC 35704 |
DNA section
IS Length : 2744 bp
Ends
IR Length : 17/22
IRL : GTAAGCGCAAAACAATTCGACGGATTGCGTTGCCCTAAAACTCAGTCCAT
IRR : GTAAGTGCAAAATATTGCTACGTTACTCATAAATTGCGCCTGCACCAAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TATTTCATAA | AGAATACCTG | 0 |
DNA sequence
GTAAGCGCAAAACAATTCGACGGATTGCGTTGCCCTAAAACTCAGTCCATAATGATTAATAGAGAATGCAAGCATTTTACTTCATCTTCTGTTGCTAGAA
ATGATAGATGCAAGCATTTTACTCTATTCGATTTATGGAGGACTGCTCATGAGAAGCAAAAGAATACCTGCTGAAGAACAATACCGTCTCATCATGGAAT
GCCGTCAAAGTGGATTGACAGATCATCAATGGTGTGCGGAACACGACATCAAGCCTGGAACCTTTTATAACTGGGTAAAGCGACTACGGCAAAAAGGTTG
CATGGATTTACCAGCGGCAACCGGACGCAGCTATAACACTCCGGAAAGCCAGGAAGTCGTCAGGGTAGATTTTCATAATCCTGACACCATCTCATATGAA
CAGCCATTGGATATGATTCCTGCGTCTATGGAAAGAAGCAGCATTTCTGAGGCAGCACCTATGAAGCTGTCTGTTGGAAGGTTTCTTCTGACTATCCCGA
ATGGAACAGATCCACAGCTTCTGGCTCAGACACTTCGCATTGTGAAAGAGCTGGAATGTTAGGGGATATCACGGTCGCCGATGAAATCTATATCGTAACA
GGCAGAACGGATATGCGGAAATCCATTGACGGACTGTGTGCCATTGTAGAGGAGCAACTTCACATGGATCCAAGACGGAGTGCCCTGTATCTTTTCTGCG
GAAGACGTTGTGACAGGATCAAAGCTTTACTCTGGGAATCCGATGGATTTGTGCTGTTGTATAAACGCATGGAAGTTCAGGGCAGATTCCGGTGGCCGAA
AAATCAGTTGGAAGTAAAACAACTGACCTGGCAGCAATTCGACTGGCTCATGTCCGGTCTTGAAATCGAACAGCCTAAAGCGTTCAAACCTACGGAATGA
TGGTGGAAAACTTCTCTGCAATCCGGTAGAAATGCGGGGCGCTCCACACCCTGGAAAGCGCCTTGCTCCCTTGTAAATACTGGGTTTTCTGCTTCTTTTT
CTTCACTTTTTGTGGTATACTAAGAGCAGAGATAAAGGAGCAGAAAACGTATGGCTGGTAATTCAAAAGATTCAAAAATCCTTGCGTATAAGGACATGAT
CAACCAACTGAATAAGACGATTGCTGCACAGACAGAACTGATCCAGTCTTTACAAAAGACATTGGAAGCAGACCGTCTGGAAAAAGAGACTCTTCGTCAA
CAGATTGAATATCTCACGAAGAAGCTCTTTGGCACTTCCAGCGAAAAACGGAAGGATATCGAAGGCCAGCTGAATCTTTTTGATGAAGCCGAGCAGGAAG
CGGATCCGGCATGGAATCCGGAACTTCCTGATGGCATCATTGTCCCGGAGCATAAGCGGAAAGCAAGACGGACTCACGCTGAACTCTTTAAAAACGTGCC
TTCCTGCGACGAGATCATTTCTCTTCCGGAGGAGGAACGGAACTGTCCAGCCTGCGGAACACAGATGGAATGTATTGGGAAAGAATTCGTCCGTCATGAA
TTCCGCTTTACCCCTGCCAAAGGTAAAGTTGTAAATATCTATCGTGAAACCTATAAGTGTCCGGAGTGTGCCATATCAGCGGAACATCCCGATGACCAGA
CTTTTGTCAAGGCTCCTGTTCAGGAACCGTTGATCCCGGAAAGTTATGCATCAGAATCTGTCGTTGCATGGGCGATGCACCAGAAGTACCAGAATGGTCT
GCCGTTAAACCGGCAGGAAGAGGACTGGAAACAACTGGGTGTACCATTGAACCGTGCTACGCTTGCCAACTGGATTATTTACTGCACCGAGAATTATCTT
CGCCATGTTTATGACTATTTTCACCGTCAGTTACTGGTGCGTAAATATCTGATGGCAGATGAAACACGGGTTCAAGTGCTGAATGAGCCGGAGCGCAATC
CGGAAACAGATTCCTGGATGTGGCTCTTCCGCAGCGGAGAAGATGGTCTTCCACCGATTCTACTCTATCATTATACAGAGACAAGAGCAAAGTTCCATGC
AGAATCATTCTTGCAAGGCTTCAGCGGATATCTGGAAACGGATGGGTATCAAGGTCATCATAATCTGCCTGATATCAAACGATGCTCTTGTTGGGCGCAT
GTGAGACGTTACTTCACAGATGCCATACCGAAGGGGAAAGAGTATAATTATAGTCTTCCCGCAGTGCAGGGAGTGCAGTTCTGTTCCAAGCTGTTTGATT
GTGAACGGTACTCAAAAGCAAAAAATCATACCGCAGAGCAGAGAAAACAGTTTCGTCTTGAAAAAGAAAAGCCGATACTGGATGTATTCTGGGATTGGCT
GGATCAACAGCACCCAAACAAGGGGACTCGTTTGGCGAAAGCGGTGAACTATGCCCAAAATCGGAAAGACACCCTGATGACCTATCTGGAAGACGGCCAC
TGCAGTTTTTCTAACAATCTCAGCGAGAATGCGATCAGACCATTCACCGTTGGCAGGAAGAACTGGTTGTTCAGTGCCAGTCCTAAAGGAGCCACCGCCA
GTGCTATTGTGTATACTATGGTTGAGATGGCAAAAGCGAATGATCTGAATACCTATAAATATCTGACATATCTCTTGTCACAACGACCGAATGATAAAAT
GTCAGATGAGCAGTTAGAGCAGTTCGCCCCATGGAGCAAGGCTGCGAAAGCTAACTGTCAAAACTAAAATAGAGTAAAACGCTTGCATCTATCATTTTGG
TGCAGGCGCAATTTATGAGTAACGTAGCAATATTTTGCACTTAC
ATGATAGATGCAAGCATTTTACTCTATTCGATTTATGGAGGACTGCTCATGAGAAGCAAAAGAATACCTGCTGAAGAACAATACCGTCTCATCATGGAAT
GCCGTCAAAGTGGATTGACAGATCATCAATGGTGTGCGGAACACGACATCAAGCCTGGAACCTTTTATAACTGGGTAAAGCGACTACGGCAAAAAGGTTG
CATGGATTTACCAGCGGCAACCGGACGCAGCTATAACACTCCGGAAAGCCAGGAAGTCGTCAGGGTAGATTTTCATAATCCTGACACCATCTCATATGAA
CAGCCATTGGATATGATTCCTGCGTCTATGGAAAGAAGCAGCATTTCTGAGGCAGCACCTATGAAGCTGTCTGTTGGAAGGTTTCTTCTGACTATCCCGA
ATGGAACAGATCCACAGCTTCTGGCTCAGACACTTCGCATTGTGAAAGAGCTGGAATGTTAGGGGATATCACGGTCGCCGATGAAATCTATATCGTAACA
GGCAGAACGGATATGCGGAAATCCATTGACGGACTGTGTGCCATTGTAGAGGAGCAACTTCACATGGATCCAAGACGGAGTGCCCTGTATCTTTTCTGCG
GAAGACGTTGTGACAGGATCAAAGCTTTACTCTGGGAATCCGATGGATTTGTGCTGTTGTATAAACGCATGGAAGTTCAGGGCAGATTCCGGTGGCCGAA
AAATCAGTTGGAAGTAAAACAACTGACCTGGCAGCAATTCGACTGGCTCATGTCCGGTCTTGAAATCGAACAGCCTAAAGCGTTCAAACCTACGGAATGA
TGGTGGAAAACTTCTCTGCAATCCGGTAGAAATGCGGGGCGCTCCACACCCTGGAAAGCGCCTTGCTCCCTTGTAAATACTGGGTTTTCTGCTTCTTTTT
CTTCACTTTTTGTGGTATACTAAGAGCAGAGATAAAGGAGCAGAAAACGTATGGCTGGTAATTCAAAAGATTCAAAAATCCTTGCGTATAAGGACATGAT
CAACCAACTGAATAAGACGATTGCTGCACAGACAGAACTGATCCAGTCTTTACAAAAGACATTGGAAGCAGACCGTCTGGAAAAAGAGACTCTTCGTCAA
CAGATTGAATATCTCACGAAGAAGCTCTTTGGCACTTCCAGCGAAAAACGGAAGGATATCGAAGGCCAGCTGAATCTTTTTGATGAAGCCGAGCAGGAAG
CGGATCCGGCATGGAATCCGGAACTTCCTGATGGCATCATTGTCCCGGAGCATAAGCGGAAAGCAAGACGGACTCACGCTGAACTCTTTAAAAACGTGCC
TTCCTGCGACGAGATCATTTCTCTTCCGGAGGAGGAACGGAACTGTCCAGCCTGCGGAACACAGATGGAATGTATTGGGAAAGAATTCGTCCGTCATGAA
TTCCGCTTTACCCCTGCCAAAGGTAAAGTTGTAAATATCTATCGTGAAACCTATAAGTGTCCGGAGTGTGCCATATCAGCGGAACATCCCGATGACCAGA
CTTTTGTCAAGGCTCCTGTTCAGGAACCGTTGATCCCGGAAAGTTATGCATCAGAATCTGTCGTTGCATGGGCGATGCACCAGAAGTACCAGAATGGTCT
GCCGTTAAACCGGCAGGAAGAGGACTGGAAACAACTGGGTGTACCATTGAACCGTGCTACGCTTGCCAACTGGATTATTTACTGCACCGAGAATTATCTT
CGCCATGTTTATGACTATTTTCACCGTCAGTTACTGGTGCGTAAATATCTGATGGCAGATGAAACACGGGTTCAAGTGCTGAATGAGCCGGAGCGCAATC
CGGAAACAGATTCCTGGATGTGGCTCTTCCGCAGCGGAGAAGATGGTCTTCCACCGATTCTACTCTATCATTATACAGAGACAAGAGCAAAGTTCCATGC
AGAATCATTCTTGCAAGGCTTCAGCGGATATCTGGAAACGGATGGGTATCAAGGTCATCATAATCTGCCTGATATCAAACGATGCTCTTGTTGGGCGCAT
GTGAGACGTTACTTCACAGATGCCATACCGAAGGGGAAAGAGTATAATTATAGTCTTCCCGCAGTGCAGGGAGTGCAGTTCTGTTCCAAGCTGTTTGATT
GTGAACGGTACTCAAAAGCAAAAAATCATACCGCAGAGCAGAGAAAACAGTTTCGTCTTGAAAAAGAAAAGCCGATACTGGATGTATTCTGGGATTGGCT
GGATCAACAGCACCCAAACAAGGGGACTCGTTTGGCGAAAGCGGTGAACTATGCCCAAAATCGGAAAGACACCCTGATGACCTATCTGGAAGACGGCCAC
TGCAGTTTTTCTAACAATCTCAGCGAGAATGCGATCAGACCATTCACCGTTGGCAGGAAGAACTGGTTGTTCAGTGCCAGTCCTAAAGGAGCCACCGCCA
GTGCTATTGTGTATACTATGGTTGAGATGGCAAAAGCGAATGATCTGAATACCTATAAATATCTGACATATCTCTTGTCACAACGACCGAATGATAAAAT
GTCAGATGAGCAGTTAGAGCAGTTCGCCCCATGGAGCAAGGCTGCGAAAGCTAACTGTCAAAACTAAAATAGAGTAAAACGCTTGCATCTATCATTTTGG
TGCAGGCGCAATTTATGAGTAACGTAGCAATATTTTGCACTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 462 bp | 153 aa | 101 | 562 | + | No |
AG : IS66 TnpA
ORF sequence :
MIDASILLYSIYGGLLMRSKRIPAEEQYRLIMECRQSGLTDHQWCAEHDIKPGTFYNWVKRLRQKGCMDLPAATGRSYNTPESQEVVRVDFHNPDTISYE
QPLDMIPASMERSSISEAAPMKLSVGRFLLTIPNGTDPQLLAQTLRIVKELEC
QPLDMIPASMERSSISEAAPMKLSVGRFLLTIPNGTDPQLLAQTLRIVKELEC
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 345 bp | 114 aa | 556 | 900 | + | No |
AG : IS66 TnpB
ORF sequence :
MLGDITVADEIYIVTGRTDMRKSIDGLCAIVEEQLHMDPRRSALYLFCGRRCDRIKALLWESDGFVLLYKRMEVQGRFRWPKNQLEVKQLTWQQFDWLMS
GLEIEQPKAFKPTE
GLEIEQPKAFKPTE
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1617 bp | 538 aa | 1051 | 2667 | + | No |
Chemistry : DDE
ORF sequence :
MAGNSKDSKILAYKDMINQLNKTIAAQTELIQSLQKTLEADRLEKETLRQQIEYLTKKLFGTSSEKRKDIEGQLNLFDEAEQEADPAWNPELPDGIIVPE
HKRKARRTHAELFKNVPSCDEIISLPEEERNCPACGTQMECIGKEFVRHEFRFTPAKGKVVNIYRETYKCPECAISAEHPDDQTFVKAPVQEPLIPESYA
SESVVAWAMHQKYQNGLPLNRQEEDWKQLGVPLNRATLANWIIYCTENYLRHVYDYFHRQLLVRKYLMADETRVQVLNEPERNPETDSWMWLFRSGEDGL
PPILLYHYTETRAKFHAESFLQGFSGYLETDGYQGHHNLPDIKRCSCWAHVRRYFTDAIPKGKEYNYSLPAVQGVQFCSKLFDCERYSKAKNHTAEQRKQ
FRLEKEKPILDVFWDWLDQQHPNKGTRLAKAVNYAQNRKDTLMTYLEDGHCSFSNNLSENAIRPFTVGRKNWLFSASPKGATASAIVYTMVEMAKANDLN
TYKYLTYLLSQRPNDKMSDEQLEQFAPWSKAAKANCQN
HKRKARRTHAELFKNVPSCDEIISLPEEERNCPACGTQMECIGKEFVRHEFRFTPAKGKVVNIYRETYKCPECAISAEHPDDQTFVKAPVQEPLIPESYA
SESVVAWAMHQKYQNGLPLNRQEEDWKQLGVPLNRATLANWIIYCTENYLRHVYDYFHRQLLVRKYLMADETRVQVLNEPERNPETDSWMWLFRSGEDGL
PPILLYHYTETRAKFHAESFLQGFSGYLETDGYQGHHNLPDIKRCSCWAHVRRYFTDAIPKGKEYNYSLPAVQGVQFCSKLFDCERYSKAKNHTAEQRKQ
FRLEKEKPILDVFWDWLDQQHPNKGTRLAKAVNYAQNRKDTLMTYLEDGHCSFSNNLSENAIRPFTVGRKNWLFSASPKGATASAIVYTMVEMAKANDLN
TYKYLTYLLSQRPNDKMSDEQLEQFAPWSKAAKANCQN
Blast result :
Comments
Genome in progress. Accession number: NZ_ABFY02000050.
ISCsc1 is 91%(orfA), 99%(orfB), and 95%(orfC) aa similar to ISRgn2.
ISCsc1 is 91%(orfA), 99%(orfB), and 95%(orfC) aa similar to ISRgn2.
References
1] ISfinder annotation (2009)
2] Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. (2007) Direct submission Genbank.
2] Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. (2007) Direct submission Genbank.