ISRob1
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AAVO00000000 | ND | Ruminococcus obeum | Ruminococcus obeum ATCC 29174 |
DNA section
IS Length : 2546 bp
Ends
IR Length : 25/30
IRL : GTAAGCGTCCAATAAGAGGGTGTATCGAAATTTCTGGCTGTTGCATGTAA
IRR : GTAAGCGTCAAATAAAACAGTGTATAGAAAAATAACCGTCAGCCTTTTAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCCCAATGGT | TTTTCATC | TTTTCGAATG | 8 |
DNA sequence
GTAAGCGTCCAATAAGAGGGTGTATCGAAATTTCTGGCTGTTGCATGTAAACTATTGATTAGAGTTTTGGGAGTTGACTCTAAAAACTCTAAATCTAATC
TAAGGAGCAGGCTTATGGATCTTTCTTCTTTGACTCCAGACAAACAGGTGAAACTTCAATACTGGCTGGATGTAATACGGCAATGCAGGGCCTCCGGACT
GACAAATCAGATCTGGTGTGAACAACACGATATCTCTTTGAAAAGTTATTATTACTGGCTTTCCAAAATTCGAAAGCTTGCCCTGGAAGAACTGCCCCGC
AAGAAAAACGGCATCCACATGTGTACAGACCAACAGTCAACTGCTTTAACGGAATCCCCGGCTGAGTTTGCGGAGCTTTCACTTCCGGACAGAAACATTT
CCCGTTCCACTCCTGCAGCCATACTTCATATCGGATCCATAACAGTGGAACTTTATGAAGGCACATCTGCCCAGATGCTGGAAAACATTCTGAAAGCCGT
GCAGTCATGTTAGGAGATCTTTCCCATGTGGAGAAAATCTATATCCGATGCGGCTACACCGATATGAGGAAGCAGCTCAATGGCTTGCTGGATATCATCC
AGTACAACTTCAAACTGGATCCTTACAGCAATTCCTTATTCCTGTTCTGTGGAAAGAGGGCTGACCGGATCAAAGCAGTCCATTACGAGGGAGACGGTTT
CTGCCTTTTATACAAACGGTACGAAAACGGACGTCTCCAATGGCCGCGGACCGGCGAAGAAGCAAAACAGATTTCTGATCAGCAGCTGCGCTGGCTTCTG
GAAGGTTTGAACCCGGAGCAGCCAAAGGCGGTTCAGAAATGGCTCCCGCATAAGTCTGAAAATACTGAAAATCCTTAGAAATGCTGGGAAATCTGACGAT
TCTATGGTATAATAATCCTATCGAAACGAAAGGGTTTTCAGGTTATGTCAGCTACGGAACAGGAGTATAAAAATCATATCAAAGAACTGGAACAGCAGGT
CCGGCTCCTGAAAGAGCAGGTTGATTTCCTGACCCGTAAACTTTATGGAACAAAATCCGAGAAGACATCTACTCTCGAAATCGAGGGGCAGGTGTCTCTT
TTTAATGAAATCGAAACCTGTGCGGATCCTGATGCACACGAACCGGAGCTTGTTGAGATAGAGAAGCACCTACGTAAAAGGAAGTACACCGGTCAGCGGG
AAGAACTGGTAAAAAATCTCCCTCACAGCAAAGTACTCCATACCATAGATGAAAGGGAACAGATATGCGATAACTGCGGGAGTACGATGGTGAAAGTAGG
GGAAGAATTCGTCCGCACGGAAGTACAGTTTATCCCTGCAAAACTCAAGGTGATTGACCATTACCGGGAAACGTATGAATGCAGGTCGTGCCGGAAAAAC
GGAACTCCTTATATGGAGAAGGCTCCGGTGCCATATCCTCCCGTCCTGCATTCTCTGGCATCTGCATCTACCATCACCTGGCTCATCCACCAGAAGTTTG
AATTAAGCATCCCGCTGTACAGACAGGAAAAAGAATGGGAGGCTTTGGGATTACGTTTAAGCAGAGCGACTATGTCAAACTGGCTTCTGATTGTCTATCG
GGACTGGCTTGTACACATCGTCCACCGTCTGAAGCTGGAACTTCTGAAACAGAGATACCTTCATATCGATGAAACACATGTACAGGTGCTGAAAGAACCG
GGACGGAAAAATACGTCCGATTCCTATATGTGGGTTTACTGCAGCATCAGAGATGCTGTAAATCCCATCCGCTACTTCGAGTACCAGCCGGGACGAAGCG
GGAAATATCCGGAAGCGTTTCTGAGGGAATATGAAGGTTATATCCATACAGACGCTTACTCCGGATACAATGCAGTTTCAGGAGTAAAAAGATGTCTTTG
CTATACGCATCTGCGCCGGGCTTTCGTGGATGCGCTCCCAAAAGACATCCATAATTCGGAAGCATCAAAACCTGCGGAAGCAATCCTCCGTCTGAATCAA
CTCTTTAACATAGAGTCAGAGTTGGAGGCGCTCCCTCCTGATCAGAAGAAAAAAGAACGTCTTATCCGCGAGAAACCGCTTCTCGAGGCTTTTTGGTCAT
GGGCAGAAAAAAGTGCCATCGGAGAGCTGCCGAAATCCAAACTTTCAAAAGCTTTTCATTACGCCTTAAATAACCGTGAAGGTTTTTGGAATTATCTGGA
AGACGGGAATTGTTCCATCAGTAATTCGCTTGCGGAAAACTGTATCCGTCCCTTTGTGATCGGCAGAAAGAACTGGCTGTTCTCCGGCAGTCCGAAAGGG
GCGGAAGCCAGTGCAGGAATCCATACACTGGTAGAGACAGCGAAAGCGAATGGGCTTGCCCCGATGAAATATATAAAATATATCCTGTCCGACATGCCGG
GGAGTGCTTTTCTTGAACATCCTGAATATCTGGATGATTATCTGCCTTGGAATCCTCTTGTAAAAGAATTCTGTCGATAACCGTTGACCTTTTAGTATAA
AAGGCTGACGGTTATTTTTCTATACACTGTTTTATTTGACGCTTAC
TAAGGAGCAGGCTTATGGATCTTTCTTCTTTGACTCCAGACAAACAGGTGAAACTTCAATACTGGCTGGATGTAATACGGCAATGCAGGGCCTCCGGACT
GACAAATCAGATCTGGTGTGAACAACACGATATCTCTTTGAAAAGTTATTATTACTGGCTTTCCAAAATTCGAAAGCTTGCCCTGGAAGAACTGCCCCGC
AAGAAAAACGGCATCCACATGTGTACAGACCAACAGTCAACTGCTTTAACGGAATCCCCGGCTGAGTTTGCGGAGCTTTCACTTCCGGACAGAAACATTT
CCCGTTCCACTCCTGCAGCCATACTTCATATCGGATCCATAACAGTGGAACTTTATGAAGGCACATCTGCCCAGATGCTGGAAAACATTCTGAAAGCCGT
GCAGTCATGTTAGGAGATCTTTCCCATGTGGAGAAAATCTATATCCGATGCGGCTACACCGATATGAGGAAGCAGCTCAATGGCTTGCTGGATATCATCC
AGTACAACTTCAAACTGGATCCTTACAGCAATTCCTTATTCCTGTTCTGTGGAAAGAGGGCTGACCGGATCAAAGCAGTCCATTACGAGGGAGACGGTTT
CTGCCTTTTATACAAACGGTACGAAAACGGACGTCTCCAATGGCCGCGGACCGGCGAAGAAGCAAAACAGATTTCTGATCAGCAGCTGCGCTGGCTTCTG
GAAGGTTTGAACCCGGAGCAGCCAAAGGCGGTTCAGAAATGGCTCCCGCATAAGTCTGAAAATACTGAAAATCCTTAGAAATGCTGGGAAATCTGACGAT
TCTATGGTATAATAATCCTATCGAAACGAAAGGGTTTTCAGGTTATGTCAGCTACGGAACAGGAGTATAAAAATCATATCAAAGAACTGGAACAGCAGGT
CCGGCTCCTGAAAGAGCAGGTTGATTTCCTGACCCGTAAACTTTATGGAACAAAATCCGAGAAGACATCTACTCTCGAAATCGAGGGGCAGGTGTCTCTT
TTTAATGAAATCGAAACCTGTGCGGATCCTGATGCACACGAACCGGAGCTTGTTGAGATAGAGAAGCACCTACGTAAAAGGAAGTACACCGGTCAGCGGG
AAGAACTGGTAAAAAATCTCCCTCACAGCAAAGTACTCCATACCATAGATGAAAGGGAACAGATATGCGATAACTGCGGGAGTACGATGGTGAAAGTAGG
GGAAGAATTCGTCCGCACGGAAGTACAGTTTATCCCTGCAAAACTCAAGGTGATTGACCATTACCGGGAAACGTATGAATGCAGGTCGTGCCGGAAAAAC
GGAACTCCTTATATGGAGAAGGCTCCGGTGCCATATCCTCCCGTCCTGCATTCTCTGGCATCTGCATCTACCATCACCTGGCTCATCCACCAGAAGTTTG
AATTAAGCATCCCGCTGTACAGACAGGAAAAAGAATGGGAGGCTTTGGGATTACGTTTAAGCAGAGCGACTATGTCAAACTGGCTTCTGATTGTCTATCG
GGACTGGCTTGTACACATCGTCCACCGTCTGAAGCTGGAACTTCTGAAACAGAGATACCTTCATATCGATGAAACACATGTACAGGTGCTGAAAGAACCG
GGACGGAAAAATACGTCCGATTCCTATATGTGGGTTTACTGCAGCATCAGAGATGCTGTAAATCCCATCCGCTACTTCGAGTACCAGCCGGGACGAAGCG
GGAAATATCCGGAAGCGTTTCTGAGGGAATATGAAGGTTATATCCATACAGACGCTTACTCCGGATACAATGCAGTTTCAGGAGTAAAAAGATGTCTTTG
CTATACGCATCTGCGCCGGGCTTTCGTGGATGCGCTCCCAAAAGACATCCATAATTCGGAAGCATCAAAACCTGCGGAAGCAATCCTCCGTCTGAATCAA
CTCTTTAACATAGAGTCAGAGTTGGAGGCGCTCCCTCCTGATCAGAAGAAAAAAGAACGTCTTATCCGCGAGAAACCGCTTCTCGAGGCTTTTTGGTCAT
GGGCAGAAAAAAGTGCCATCGGAGAGCTGCCGAAATCCAAACTTTCAAAAGCTTTTCATTACGCCTTAAATAACCGTGAAGGTTTTTGGAATTATCTGGA
AGACGGGAATTGTTCCATCAGTAATTCGCTTGCGGAAAACTGTATCCGTCCCTTTGTGATCGGCAGAAAGAACTGGCTGTTCTCCGGCAGTCCGAAAGGG
GCGGAAGCCAGTGCAGGAATCCATACACTGGTAGAGACAGCGAAAGCGAATGGGCTTGCCCCGATGAAATATATAAAATATATCCTGTCCGACATGCCGG
GGAGTGCTTTTCTTGAACATCCTGAATATCTGGATGATTATCTGCCTTGGAATCCTCTTGTAAAAGAATTCTGTCGATAACCGTTGACCTTTTAGTATAA
AAGGCTGACGGTTATTTTTCTATACACTGTTTTATTTGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 399 bp | 132 aa | 115 | 513 | + | No |
AG : IS66 TnpA
ORF sequence :
MDLSSLTPDKQVKLQYWLDVIRQCRASGLTNQIWCEQHDISLKSYYYWLSKIRKLALEELPRKKNGIHMCTDQQSTALTESPAEFAELSLPDRNISRSTP
AAILHIGSITVELYEGTSAQMLENILKAVQSC
AAILHIGSITVELYEGTSAQMLENILKAVQSC
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 372 bp | 123 aa | 507 | 878 | + | No |
AG : IS66 TnpB
ORF sequence :
MLGDLSHVEKIYIRCGYTDMRKQLNGLLDIIQYNFKLDPYSNSLFLFCGKRADRIKAVHYEGDGFCLLYKRYENGRLQWPRTGEEAKQISDQQLRWLLEG
LNPEQPKAVQKWLPHKSENTENP
LNPEQPKAVQKWLPHKSENTENP
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1536 bp | 511 aa | 945 | 2480 | + | No |
Chemistry : DDE
ORF sequence :
MSATEQEYKNHIKELEQQVRLLKEQVDFLTRKLYGTKSEKTSTLEIEGQVSLFNEIETCADPDAHEPELVEIEKHLRKRKYTGQREELVKNLPHSKVLHT
IDEREQICDNCGSTMVKVGEEFVRTEVQFIPAKLKVIDHYRETYECRSCRKNGTPYMEKAPVPYPPVLHSLASASTITWLIHQKFELSIPLYRQEKEWEA
LGLRLSRATMSNWLLIVYRDWLVHIVHRLKLELLKQRYLHIDETHVQVLKEPGRKNTSDSYMWVYCSIRDAVNPIRYFEYQPGRSGKYPEAFLREYEGYI
HTDAYSGYNAVSGVKRCLCYTHLRRAFVDALPKDIHNSEASKPAEAILRLNQLFNIESELEALPPDQKKKERLIREKPLLEAFWSWAEKSAIGELPKSKL
SKAFHYALNNREGFWNYLEDGNCSISNSLAENCIRPFVIGRKNWLFSGSPKGAEASAGIHTLVETAKANGLAPMKYIKYILSDMPGSAFLEHPEYLDDYL
PWNPLVKEFCR
IDEREQICDNCGSTMVKVGEEFVRTEVQFIPAKLKVIDHYRETYECRSCRKNGTPYMEKAPVPYPPVLHSLASASTITWLIHQKFELSIPLYRQEKEWEA
LGLRLSRATMSNWLLIVYRDWLVHIVHRLKLELLKQRYLHIDETHVQVLKEPGRKNTSDSYMWVYCSIRDAVNPIRYFEYQPGRSGKYPEAFLREYEGYI
HTDAYSGYNAVSGVKRCLCYTHLRRAFVDALPKDIHNSEASKPAEAILRLNQLFNIESELEALPPDQKKKERLIREKPLLEAFWSWAEKSAIGELPKSKL
SKAFHYALNNREGFWNYLEDGNCSISNSLAENCIRPFVIGRKNWLFSGSPKGAEASAGIHTLVETAKANGLAPMKYIKYILSDMPGSAFLEHPEYLDDYL
PWNPLVKEFCR
Blast result :
Comments
ISRob1 is 55%(orfA), 81%(orfB), and 75%(orfC) aa similar to ISCth11.
References
1] ISfinder annotation (2009)
2] Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. (2007) Direct submission GenBank.
2] Fulton,L., Clifton,S., Fulton,B., Xu,J., Minx,P., Pepin,K.H., Johnson,M., Thiruvilangam,P., Bhonagiri,V., Nash,W.E., Mardis,E.R. and Wilson,R.K. (2007) Direct submission GenBank.