ISCce5
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_011898 | ND | Clostridium cellulolyticum | Clostridium cellulolyticum H10 |
DNA section
IS Length : 2496 bp
Ends
IR Length : 23
IRL : GTAAGCGCTTAATCTTGGGGTGCATAGTAGTTTATCCATCTGTATGAGAT
IRR : GTAAGCGCTTAATCTTGGGGTGCCTTGTTTGAATCTTTATGACACCAGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ATTATGACTG | CAAATAAG | TATTGTTCTG | 8 |
| TTTCTAATTC | AGCTAAAC | TATGTACATC | 8 |
| CAATTTGGCC | TTCTCACG | AGCTGATGCC | 8 |
| CAATCTTATT | TGTTTTTTGG | 0 |
DNA sequence
GTAAGCGCTTAATCTTGGGGTGCATAGTAGTTTATCCATCTGTATGAGATAATCCTTTCAAATTGTCTTTAGACTTTTTAAAGTTTTTCGACTTTGTCTA
AAAAGTCTGAAATCACCAAGGAGGCTATCCCCAAATGGATAAAATAACAGATACTAAAACTGAATTTAGACTCAAGCAGTGGACTCAGATAGTCCAGACG
TGCCAAGCCAGTGGAATGACGGCTGTCAGTTGGTGTAATCAGAACAATGTAAATATCAAATCATATTACTATTGGTTACGCAGAATCCGCACTCTGACTA
TCGAAAATGGACCTCTTGAGCTTCAAAGTAAAGGACAGCAGATTGTACCCGTATCTTTCCGGCAGACAGCAGCAGTTACAATTCATATTAATTCCGTTTC
TATAGATATCCCTGATGGAACTTCCAAAGATACAATTGCGGCAGTTCTGTCGGCATTGAAGACCATATGTTAGGCGATATTTCAAAAGCAGAAGACATTT
ACATTGTATGTGGTTACTCTGATATGCGCAAGTCAATCGACGGATTCGCCGCCATCATAAAGGGAACTTTTGGTATGGATCCGTTTTCACCGAGCCTGTT
TTTGTTCTGCGGTAAAAGAAGGGATCGGTTAAAAGCTCTCTATTGGGAAGGGGACGGTTTTGTCCTGCTGTACAAGCGTCTGGAGAACGGGAGCTTCAAA
TGGCCTCGGACACCAGAGCAGGCAAGACGTCTTACAATGCAGGAATTTCGGTGGCTTATGGATGGCTTGGCTATTGATCAACCTAAGGCAATCCGGGAAG
CGAAACAGGGTGATATCTATTGATAAAAAGTGCTGAAAAGTGTGGGTTCTACTGTATTTTAGTTGGAATTTTCAGTCCAATAATGGTATAATTAACTTAT
GCAAAAGATTGATATTACAGGGCTTTCGCCCAATCAAATTGAATATGTTTCCTCTTTGGAAAAAACAGTAGAAAACCAGCAAGTTCGTATTGAACAACTT
ACTGAGCTTCTTATTAAATCTCAAAAAGCTTTATATGGTCAATCCAGCGAAAAGCGGCGTTATGTTTTTGATGAAGACAGCGGTCAGCTTTCCTTATTCA
ATGAGGCAGAGGTCGAAGCTGGCAACAAGGCAGAAGAACCGACCGTGCAGACAATTGTGGCAGCACATACCAGAAAACCCAAGCGAACCAAGGAAGAACT
GGCAGAAACGGTACCAGTGGTGGAAGTCGTTTGTGATCTGGATAAAGATAAACGCACCTGTAACATTTGCAACACTGATCTGAGATATCTCGGAAAGGAA
TATGTCCGGGACGAACTGGAGATTATCCCTGCCCAGGTACGCATATTGAGATATATTCGTTTAAATTATGTATGCAAGGAATGTGAGAAGGAGACCTGCG
AAGCCAATATTGTTAAAGCTCCTGTCCCAAAACCTGTTATGAAGCGCAGCCTTGCTTCTGCCTCAACCGTAGCTTATGTAATGTATCAAAAATATGCAAA
TGGAATGCCTTTATACCGACAGGAAAAAGACTGGGCGAACCAAGGAGTGAAACTCTCCAGAGCTACTCTGGCCAATTGGATTATACGTCCAAGTCATGAA
TGGCTTGAACCAATGTATGATGCTATTAAGAAAAATCTGGTGACCGAACCGTTGATACATGCGGACGAAACCGTCCTGCAGGTATTGAAAGAACCGGGAC
GGAGAGCAACCACCGAATCTCGAATGTGGGTCTACACTTCCGGTCAAAGTCTGACTCCGGCGGTTTTGTTTGAATACCAGCCGACACGATCCGGTCAGCA
TGCCAGACGGTTTCTGGAAGGGTTTTCGGGTTATCTTCAAACAGATGGCTATAGTGGTTACAATGCAGTACCTAATGTCATACATTGTGGATGTTGGGCT
CATTTACAGCGTAAATTTGAAGAAGCAATTCCTAATGGAGCAGATAACAAAAGCTCCAAGGCGGCTATTGGATATGATTACTGTAACCGCTTATTTGCCA
TGGAAAAGAAATGGATAGAATTATCCTCTGAAAATCGGCACCAAGAACGGCGAAAAAATGCAAAACCTCTTCTTGATGAGTTCTGGCAATGGGTATCGCA
TTTAAATCCTCTCCAAAACTCAAATCTGGGAAAAGCAGTTACCTATGCGTTGAATCAAAAGGAAACTCTGATTAATTTTATGCTGGATGGGTGCATCGAA
ATTTCCAATAACCGTGCTGAAAATGCCATCAGGCCTTATGTCACAGGTCGGAAGAACTGGCTCTTTGCCGACACGACCCGTGGAGCCAAAGCCAGTGCCA
TTGTATACAGTATGATCGAATCAGCTAAAGCAAATCAACTCAATCCATATATGTATTTGGTGTATCTACTGTCAAAATTACCGGACTTGAAAGAATTAAC
GCAGAAATCTCTGACACCTTATCTGCCATGGTCACCCGAATTGCCATGCTGGTGTCATAAAGATTCAAACAAGGCACCCCAAGATTAAGCGCTTAC
AAAAGTCTGAAATCACCAAGGAGGCTATCCCCAAATGGATAAAATAACAGATACTAAAACTGAATTTAGACTCAAGCAGTGGACTCAGATAGTCCAGACG
TGCCAAGCCAGTGGAATGACGGCTGTCAGTTGGTGTAATCAGAACAATGTAAATATCAAATCATATTACTATTGGTTACGCAGAATCCGCACTCTGACTA
TCGAAAATGGACCTCTTGAGCTTCAAAGTAAAGGACAGCAGATTGTACCCGTATCTTTCCGGCAGACAGCAGCAGTTACAATTCATATTAATTCCGTTTC
TATAGATATCCCTGATGGAACTTCCAAAGATACAATTGCGGCAGTTCTGTCGGCATTGAAGACCATATGTTAGGCGATATTTCAAAAGCAGAAGACATTT
ACATTGTATGTGGTTACTCTGATATGCGCAAGTCAATCGACGGATTCGCCGCCATCATAAAGGGAACTTTTGGTATGGATCCGTTTTCACCGAGCCTGTT
TTTGTTCTGCGGTAAAAGAAGGGATCGGTTAAAAGCTCTCTATTGGGAAGGGGACGGTTTTGTCCTGCTGTACAAGCGTCTGGAGAACGGGAGCTTCAAA
TGGCCTCGGACACCAGAGCAGGCAAGACGTCTTACAATGCAGGAATTTCGGTGGCTTATGGATGGCTTGGCTATTGATCAACCTAAGGCAATCCGGGAAG
CGAAACAGGGTGATATCTATTGATAAAAAGTGCTGAAAAGTGTGGGTTCTACTGTATTTTAGTTGGAATTTTCAGTCCAATAATGGTATAATTAACTTAT
GCAAAAGATTGATATTACAGGGCTTTCGCCCAATCAAATTGAATATGTTTCCTCTTTGGAAAAAACAGTAGAAAACCAGCAAGTTCGTATTGAACAACTT
ACTGAGCTTCTTATTAAATCTCAAAAAGCTTTATATGGTCAATCCAGCGAAAAGCGGCGTTATGTTTTTGATGAAGACAGCGGTCAGCTTTCCTTATTCA
ATGAGGCAGAGGTCGAAGCTGGCAACAAGGCAGAAGAACCGACCGTGCAGACAATTGTGGCAGCACATACCAGAAAACCCAAGCGAACCAAGGAAGAACT
GGCAGAAACGGTACCAGTGGTGGAAGTCGTTTGTGATCTGGATAAAGATAAACGCACCTGTAACATTTGCAACACTGATCTGAGATATCTCGGAAAGGAA
TATGTCCGGGACGAACTGGAGATTATCCCTGCCCAGGTACGCATATTGAGATATATTCGTTTAAATTATGTATGCAAGGAATGTGAGAAGGAGACCTGCG
AAGCCAATATTGTTAAAGCTCCTGTCCCAAAACCTGTTATGAAGCGCAGCCTTGCTTCTGCCTCAACCGTAGCTTATGTAATGTATCAAAAATATGCAAA
TGGAATGCCTTTATACCGACAGGAAAAAGACTGGGCGAACCAAGGAGTGAAACTCTCCAGAGCTACTCTGGCCAATTGGATTATACGTCCAAGTCATGAA
TGGCTTGAACCAATGTATGATGCTATTAAGAAAAATCTGGTGACCGAACCGTTGATACATGCGGACGAAACCGTCCTGCAGGTATTGAAAGAACCGGGAC
GGAGAGCAACCACCGAATCTCGAATGTGGGTCTACACTTCCGGTCAAAGTCTGACTCCGGCGGTTTTGTTTGAATACCAGCCGACACGATCCGGTCAGCA
TGCCAGACGGTTTCTGGAAGGGTTTTCGGGTTATCTTCAAACAGATGGCTATAGTGGTTACAATGCAGTACCTAATGTCATACATTGTGGATGTTGGGCT
CATTTACAGCGTAAATTTGAAGAAGCAATTCCTAATGGAGCAGATAACAAAAGCTCCAAGGCGGCTATTGGATATGATTACTGTAACCGCTTATTTGCCA
TGGAAAAGAAATGGATAGAATTATCCTCTGAAAATCGGCACCAAGAACGGCGAAAAAATGCAAAACCTCTTCTTGATGAGTTCTGGCAATGGGTATCGCA
TTTAAATCCTCTCCAAAACTCAAATCTGGGAAAAGCAGTTACCTATGCGTTGAATCAAAAGGAAACTCTGATTAATTTTATGCTGGATGGGTGCATCGAA
ATTTCCAATAACCGTGCTGAAAATGCCATCAGGCCTTATGTCACAGGTCGGAAGAACTGGCTCTTTGCCGACACGACCCGTGGAGCCAAAGCCAGTGCCA
TTGTATACAGTATGATCGAATCAGCTAAAGCAAATCAACTCAATCCATATATGTATTTGGTGTATCTACTGTCAAAATTACCGGACTTGAAAGAATTAAC
GCAGAAATCTCTGACACCTTATCTGCCATGGTCACCCGAATTGCCATGCTGGTGTCATAAAGATTCAAACAAGGCACCCCAAGATTAAGCGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 339 bp | 112 aa | 135 | 473 | + | No |
AG : IS66 TnpA
ORF sequence :
MDKITDTKTEFRLKQWTQIVQTCQASGMTAVSWCNQNNVNIKSYYYWLRRIRTLTIENGPLELQSKGQQIVPVSFRQTAAVTIHINSVSIDIPDGTSKDT
IAAVLSALKTIC
IAAVLSALKTIC
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 357 bp | 118 aa | 467 | 823 | + | No |
AG : IS66 TnpB
ORF sequence :
MLGDISKAEDIYIVCGYSDMRKSIDGFAAIIKGTFGMDPFSPSLFLFCGKRRDRLKALYWEGDGFVLLYKRLENGSFKWPRTPEQARRLTMQEFRWLMDG
LAIDQPKAIREAKQGDIY
LAIDQPKAIREAKQGDIY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1590 bp | 529 aa | 899 | 2488 | + | No |
Chemistry : DDE
ORF sequence :
MQKIDITGLSPNQIEYVSSLEKTVENQQVRIEQLTELLIKSQKALYGQSSEKRRYVFDEDSGQLSLFNEAEVEAGNKAEEPTVQTIVAAHTRKPKRTKEE
LAETVPVVEVVCDLDKDKRTCNICNTDLRYLGKEYVRDELEIIPAQVRILRYIRLNYVCKECEKETCEANIVKAPVPKPVMKRSLASASTVAYVMYQKYA
NGMPLYRQEKDWANQGVKLSRATLANWIIRPSHEWLEPMYDAIKKNLVTEPLIHADETVLQVLKEPGRRATTESRMWVYTSGQSLTPAVLFEYQPTRSGQ
HARRFLEGFSGYLQTDGYSGYNAVPNVIHCGCWAHLQRKFEEAIPNGADNKSSKAAIGYDYCNRLFAMEKKWIELSSENRHQERRKNAKPLLDEFWQWVS
HLNPLQNSNLGKAVTYALNQKETLINFMLDGCIEISNNRAENAIRPYVTGRKNWLFADTTRGAKASAIVYSMIESAKANQLNPYMYLVYLLSKLPDLKEL
TQKSLTPYLPWSPELPCWCHKDSNKAPQD
LAETVPVVEVVCDLDKDKRTCNICNTDLRYLGKEYVRDELEIIPAQVRILRYIRLNYVCKECEKETCEANIVKAPVPKPVMKRSLASASTVAYVMYQKYA
NGMPLYRQEKDWANQGVKLSRATLANWIIRPSHEWLEPMYDAIKKNLVTEPLIHADETVLQVLKEPGRRATTESRMWVYTSGQSLTPAVLFEYQPTRSGQ
HARRFLEGFSGYLQTDGYSGYNAVPNVIHCGCWAHLQRKFEEAIPNGADNKSSKAAIGYDYCNRLFAMEKKWIELSSENRHQERRKNAKPLLDEFWQWVS
HLNPLQNSNLGKAVTYALNQKETLINFMLDGCIEISNNRAENAIRPYVTGRKNWLFADTTRGAKASAIVYSMIESAKANQLNPYMYLVYLLSKLPDLKEL
TQKSLTPYLPWSPELPCWCHKDSNKAPQD
Blast result :
Comments
ISCce5 is 54%(orfA), 84%(orfB), and 64%(orfC) aa similar to ISCth11.
References
1]ISfinder annotation (2009)
2]Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Zhou,J. and Richardson,P. (2009) Direct submission Genbank.
2]Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Saunders,E., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Zhou,J. and Richardson,P. (2009) Direct submission Genbank.