ISfinder Mirror Site

ISPam3

Family IS3
Group IS407

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010847	ND	Paracoccus aminophilus	Paracoccus aminophilus plasmid pAMI2

DNA section

IS Length : 1197 bp

Ends

IR Length : 30/39

IRL : GGTAATCCTCCCGATGGTTAAGGGGATGCGGAAGTAGAATTTTCTCGGCA
IRR : GGTAATCCTCCCCGTTTTAACGGGGTGCGACCGTAGAACTCACGCGGCCA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATGGGCAGTG	ATAC	AGAAAAGTGA	4
CAGAAGAGAA	GGAC	GATCGGCGAG	4

DNA sequence

GGTAATCCTCCCGATGGTTAAGGGGATGCGGAAGTAGAATTTTCTCGGCAGGATCGACGAGGAGATTCTGATGAAGAAGACGAGATTTACCGAGGCACAG
ATCATGCGCGTGCTGCGACAGGCCGAGGGCGGTATGCCCGTGGGCGAGTTGTGCCGCGAGCATGGGATCAGCAGCGCGACGTTCTATAAGTGGCGGGCGA
AGTATGGCGGCATGGACGTGTCAATGATGAGCCAGATGAAGGCGCTCGAAGACGAGAACCGGCGGCTGAAACGCATGTTCGCCGATCTGAGCATGCAGGC
CGACCTGCTTCGAGAGGCTCTTGGAAAAAAGTGACGCGGCCAGCTCGACGCCGGGAGTTGGCCGAGAAGGCGGTGGCGACGAAGGGTGTCAGCATCGCGC
TGGCGTGCCGCGCGTTCGGCGTCAGCGAGACCTGTTTCCGCTACAGTCCGAGGCGCGACGAAGAGAACGAGATGATCGCCGACCTGCTGGTGGGGCTGAC
CAATGTCCACAAGACGTGGGGGTTCGGGCTGTGCTTCCTCCACCTGCGGAACGTGAAGGGGCATGTCTGGAACCACAAGCGGGTTCACCGGATCTACTGC
GAACTGGAGCTGAACCTGCGCATCAAGCCGCGGCGACGGCTGAAGCGCGAGAAGCCCGAGGAACTGGCCGTGCCGGATGCCCCGAACCTGGTCTGGTCGA
TGGATTTCATGGCGGACCGCTTGGCCGACGGGCGGCAATTCCGGCTTCTGAACGTGCTAGACGACTTCAATCGCGAGGGCCTGGGGATCGAGGTCGACTT
CTCGCTGCCCGCCGAACGGGTCGTCCGCTCCCTGAACCAGATCATCGAATGGCGCGGAAAGCCCCTGGCGATCAGGGTCGACAACGGCCCCGAATACGTC
AGTTCCACGCTGATGACCTGGGCCGAGAAGCAGGGCATCGCCCTGACATACATCCAGCCCGGCAAGCCGCAGCAGAACGCCTATGTCGAGCGTTACAACC
GGACGGTCCGCCACGAATGGCTCGACCTCTACATCTTTGAAACCATCGAGGAGGTGCAGCAGATCGCCACCGAATGGCTATGGACCTACAACAACGAACG
CCCGAATATGGGCATCGGCGGCGTTACACCCGCCATGAAGCTGAAAATGGCCGCGTGAGTTCTACGGTCGCACCCCGTTAAAACGGGGAGGATTACC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
264 bp	87 aa	71	334	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKKTRFTEAQIMRVLRQAEGGMPVGELCREHGISSATFYKWRAKYGGMDVSMMSQMKALEDENRRLKRMFADLSMQADLLREALGKK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
969 bp	322 aa	190	1158	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VAGEVWRHGRVNDEPDEGARRREPAAETHVRRSEHAGRPASRGSWKKVTRPARRRELAEKAVATKGVSIALACRAFGVSETCFRYSPRRDEENEMIADLL
VGLTNVHKTWGFGLCFLHLRNVKGHVWNHKRVHRIYCELELNLRIKPRRRLKREKPEELAVPDAPNLVWSMDFMADRLADGRQFRLLNVLDDFNREGLGI
EVDFSLPAERVVRSLNQIIEWRGKPLAIRVDNGPEYVSSTLMTWAEKQGIALTYIQPGKPQQNAYVERYNRTVRHEWLDLYIFETIEEVQQIATEWLWTY
NNERPNMGIGGVTPAMKLKMAA

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1088 bp	362 aa	71	1158	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKKTRFTEAQIMRVLRQAEGGMPVGELCREHGISSATFYKWRAKYGGMDVSMMSQMKALEDENRRLKRMFADLSMQADLLREALGKKVTRPARRRELAEK
AVATKGVSIALACRAFGVSETCFRYSPRRDEENEMIADLLVGLTNVHKTWGFGLCFLHLRNVKGHVWNHKRVHRIYCELELNLRIKPRRRLKREKPEELA
VPDAPNLVWSMDFMADRLADGRQFRLLNVLDDFNREGLGIEVDFSLPAERVVRSLNQIIEWRGKPLAIRVDNGPEYVSSTLMTWAEKQGIALTYIQPGKP
QQNAYVERYNRTVRHEWLDLYIFETIEEVQQIATEWLWTYNNERPNMGIGGVTPAMKLKMAA

Blast result :

Comments

References

1] Dziewit,L., Jazurek,M., Drewniak,L., Baj,J. and Bartosik,D. (2007) J. Bacteriol. 189 (5), 1983-1997
2] Dziewit, L., Dmowski, M., Baj, J., and Bartosik, D. (2010) Appl. Environ. Microbiol. 76, 1861-1869.
3] Dziewit, L., Baj, J., Szuplewska, M., Maj A., Tabin, M., Czyzkowska, A., Skrzypczyk, G., Adamczuk, M., Sitarek, T., Stawinski, P., Tudek, A., Wanasz, K., Wardal, E., Piechucka, E., Bartosik, D. (2012) PLoS ONE 7: e32277