ISYal1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Yersinia aldovae | Yersinia aldovae ATCC 35236 |
DNA section
IS Length : 1187 bp
Ends
IR Length : 34/43
IRL : GTCCACTCCCCCTAAAATGAGACAGCTATAATTAGATTTTCTGCCTTTAC
IRR : GTCCACTCCCCGTTATCTGAGACAGTCCTGATTAGAGTTTTCTAGCTGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GTCCACTCCCCCTAAAATGAGACAGCTATAATTAGATTTTCTGCCTTTACCTTTGCAGAGGTCAAAATGAAAAAAGCACGCTTTACTGAAACTCAGATCG
TTAATATCCTGAAACTGGCTGATTCAGGTATAAAAGTTGAAGAAATTTGCCGTCAGAACGGAATTAGCAGTGCCACCTACTACAATTGGAAATCTAAGTA
TGGTGGGATGGAAGCTAATGATGTTAAACGATTAAAAGAGCTTGAAGAGGAAAATACTCGTCTCAAAAAATTGTTTGCTGAAGTCAGTCTTGAAAATCAT
GCCATGAAGGAGCTTTTTGCAAAAAAGGGTTGGTAATAACCGAGAAAAAGTCCTGTGCCGGATTATTAACGGCTTCAGGCCTGTCGGTTATTACAGCTTG
CAAACTGACCTCTTTATCGCGGGCCTCTTTTTATCGGCGCGGTACTGACTGGCGAGAAAAAGATAAGGTCGTTATCGATGCCATTCAGGCTGTGTTGTCT
GAGTCGCCTCAGGCTGGTTTCTGGAAATGCTATTATCGTCTCAGATTTAAAGGGTTTATCTTCAACCATAAACGTGTTTATCGTGTGTATTGCCGGTTAG
GTTTAAACCTTAAACGCAGGATCAAAAAAACGCTGCCAAAACGTGAGAATAAGCCGTTATCAATTGTGAATCTGCCTGATATTCAGTGGGCACTGGACTT
TATGCATGATGCTCTTTACTGCGGTAAGCGGTTCAGAACACTAAATATAATTGACGAAGGAACGCGTGAATGTCTGGCCATTGAAGTTGATACTTCTTTA
CCGACAGACCGGGTTATCCGTGTACTTGATCGACTGAAAAAAGAACGTGGGTTACCTCAGCAACTCAGAGTTGATAATGGCCCAGAACTGATATCAGTTA
ACTTATTGAATTACTGCGAATATAATCACATTACACTGTGCCATATTCAGCCGGGTAAACCACAACAAAACGGATTTATTGAGCGTTTTAATGGCTCATT
TCGTCGGGAGTTTTTGAATGCTTACTTGTTTGAATCATTAAGCCAAGTCAGAGAAATGGCTTGGTTCTGGCAACAGGATTATAACCTGAATCGTACACAT
GAAAGTCTGGGGCATCTTCCCCCGGAGACATACCGAAAACAGCTAGAAAACTCTAATCAGGACTGTCTCAGATAACGGGGAGTGGAC
TTAATATCCTGAAACTGGCTGATTCAGGTATAAAAGTTGAAGAAATTTGCCGTCAGAACGGAATTAGCAGTGCCACCTACTACAATTGGAAATCTAAGTA
TGGTGGGATGGAAGCTAATGATGTTAAACGATTAAAAGAGCTTGAAGAGGAAAATACTCGTCTCAAAAAATTGTTTGCTGAAGTCAGTCTTGAAAATCAT
GCCATGAAGGAGCTTTTTGCAAAAAAGGGTTGGTAATAACCGAGAAAAAGTCCTGTGCCGGATTATTAACGGCTTCAGGCCTGTCGGTTATTACAGCTTG
CAAACTGACCTCTTTATCGCGGGCCTCTTTTTATCGGCGCGGTACTGACTGGCGAGAAAAAGATAAGGTCGTTATCGATGCCATTCAGGCTGTGTTGTCT
GAGTCGCCTCAGGCTGGTTTCTGGAAATGCTATTATCGTCTCAGATTTAAAGGGTTTATCTTCAACCATAAACGTGTTTATCGTGTGTATTGCCGGTTAG
GTTTAAACCTTAAACGCAGGATCAAAAAAACGCTGCCAAAACGTGAGAATAAGCCGTTATCAATTGTGAATCTGCCTGATATTCAGTGGGCACTGGACTT
TATGCATGATGCTCTTTACTGCGGTAAGCGGTTCAGAACACTAAATATAATTGACGAAGGAACGCGTGAATGTCTGGCCATTGAAGTTGATACTTCTTTA
CCGACAGACCGGGTTATCCGTGTACTTGATCGACTGAAAAAAGAACGTGGGTTACCTCAGCAACTCAGAGTTGATAATGGCCCAGAACTGATATCAGTTA
ACTTATTGAATTACTGCGAATATAATCACATTACACTGTGCCATATTCAGCCGGGTAAACCACAACAAAACGGATTTATTGAGCGTTTTAATGGCTCATT
TCGTCGGGAGTTTTTGAATGCTTACTTGTTTGAATCATTAAGCCAAGTCAGAGAAATGGCTTGGTTCTGGCAACAGGATTATAACCTGAATCGTACACAT
GAAAGTCTGGGGCATCTTCCCCCGGAGACATACCGAAAACAGCTAGAAAACTCTAATCAGGACTGTCTCAGATAACGGGGAGTGGAC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 270 bp | 89 aa | 67 | 336 | + | No |
Description : First part of the transposase
ORF sequence :
MKKARFTETQIVNILKLADSGIKVEEICRQNGISSATYYNWKSKYGGMEANDVKRLKELEEENTRLKKLFAEVSLENHAMKELFAKKGW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 882 bp | 293 aa | 294 | 1175 | + | No |
Description : Second part of the transposase
ORF sequence :
KSCHEGAFCKKGLVITEKKSCAGLLTASGLSVITACKLTSLSRASFYRRGTDWREKDKVVIDAIQAVLSESPQAGFWKCYYRLRFKGFIFNHKRVYRVYC
RLGLNLKRRIKKTLPKRENKPLSIVNLPDIQWALDFMHDALYCGKRFRTLNIIDEGTRECLAIEVDTSLPTDRVIRVLDRLKKERGLPQQLRVDNGPELI
SVNLLNYCEYNHITLCHIQPGKPQQNGFIERFNGSFRREFLNAYLFESLSQVREMAWFWQQDYNLNRTHESLGHLPPETYRKQLENSNQDCLR
RLGLNLKRRIKKTLPKRENKPLSIVNLPDIQWALDFMHDALYCGKRFRTLNIIDEGTRECLAIEVDTSLPTDRVIRVLDRLKKERGLPQQLRVDNGPELI
SVNLLNYCEYNHITLCHIQPGKPQQNGFIERFNGSFRREFLNAYLFESLSQVREMAWFWQQDYNLNRTHESLGHLPPETYRKQLENSNQDCLR
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1109 bp | 369 aa | 67 | 1175 | + | Yes |
Chemistry : DDE
ORF sequence :
MKKARFTETQIVNILKLADSGIKVEEICRQNGISSATYYNWKSKYGGMEANDVKRLKELEEENTRLKKLFAEVSLENHAMKELFAKKGLVITEKKSCAGL
LTASGLSVITACKLTSLSRASFYRRGTDWREKDKVVIDAIQAVLSESPQAGFWKCYYRLRFKGFIFNHKRVYRVYCRLGLNLKRRIKKTLPKRENKPLSI
VNLPDIQWALDFMHDALYCGKRFRTLNIIDEGTRECLAIEVDTSLPTDRVIRVLDRLKKERGLPQQLRVDNGPELISVNLLNYCEYNHITLCHIQPGKPQ
QNGFIERFNGSFRREFLNAYLFESLSQVREMAWFWQQDYNLNRTHESLGHLPPETYRKQLENSNQDCLR
LTASGLSVITACKLTSLSRASFYRRGTDWREKDKVVIDAIQAVLSESPQAGFWKCYYRLRFKGFIFNHKRVYRVYCRLGLNLKRRIKKTLPKRENKPLSI
VNLPDIQWALDFMHDALYCGKRFRTLNIIDEGTRECLAIEVDTSLPTDRVIRVLDRLKKERGLPQQLRVDNGPELISVNLLNYCEYNHITLCHIQPGKPQ
QNGFIERFNGSFRREFLNAYLFESLSQVREMAWFWQQDYNLNRTHESLGHLPPETYRKQLENSNQDCLR
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISYal1 is 85% aa similar to ISAma2.
This IS has atypic IRs: it begins with 'GT' instead of 'TG'.
ISYal1 is 85% aa similar to ISAma2.
This IS has atypic IRs: it begins with 'GT' instead of 'TG'.
References
1] Timothy Read (2009) Direct submission