ISBth10
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Bacillus thuringiensis | Bacillus thuringiensis subsp. kurstaki YBT-1520 |
DNA section
IS Length : 1434 bp
Ends
IR Length : 20/25
IRL : TGAAGTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTGCATTT
IRR : TGAAGTGACCCCCGAAAGTTAGACAGGTCGTTTCATTAGGCAGCCTCTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCATCTCAG | CAC | CCTGAACGGC | 3 |
DNA sequence
TGAAGTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTGCATTTTTTATGGCTAAATTTTCTTCAAAAGAAAAAATCCAAGCGGTGAAACGATA
TTTAGATGGTACAGAAAGTGGAAAAACAATTGCTAAATCTATAGGAGTTAACCCTAGCGTACTTCGTGAGTGGATTAGACGATATGAGTCTTCAGGTGAA
AAGGCCTTTGAAAAGTGCTATACATTCTACCCAGCCCAGTATAAACTAGATGTACTTTATTATATGAATGAACACGGGACATCTATCAGAGAAACAGCGG
CACTTTTCAATATACCGTCTTATGAAACACTTCGGAAATGGAAAATAGCTTATGAAACAGGAGGATTGGATGCCCTACAATCAAAGAAAAAGGGGCGTCC
AACCATGAAAGATAAAAAAATAAAGCCAGTAGATGAAGGATCAATCGAAGCCTTACAAGCGGAAAATGAGCGTTTACGTATGGAAAATGCATATTTAAAA
AAGTTGAATGCCTTAGTTCAAAACAAAGAAAAATCACCAAACAAGACAAAGCGCAAGTAATCTACGAGTTAAGGCATGAATTTCCTATCAAGGAGTTACT
TCAACTCGCAAACATTCCACGTAGTACGTACTATTACTGGATGAAACGTTTTAATCGTCCTGATCCAAATGCGGAGGTAAAAGAACTGATTCAAGCTATT
TATAATGAACACGATGGGTGTTATGGTTATCGTCGTATTCGTGATGAACTTATGAATCGTGGACACAAAGTAAATCATAAAAAAGTGTATCGCCTTATGA
AAGAATTAGGGTTAAAATGTCTGGTTCGTATGAAAAAATACCGCTCTTACAAAGGGACAGTTGGGAAAATTGCGCCAAATATTTTAAATCGCAACTTCCA
AGCTGAAAAACCAAATGAAAAGTGGGTTACAGATATTACGGAGTTTAAGTTATTTGGGGAGAAGTTATACCTATCACCAATGTTGGATTTATTTAATGGT
GAAATTATCACGTATACAATTGGTTCAAGACCGACCTATTCCCTTGTTTCAACGATGTTGGACCAAGCCCTTTATTGTGTAACCAATCCGGATAAACTCC
TCATTCACTCTGATCAAGGTTGGCACTATCAAATGAAACAATACCGTCATTCTCTTAAGAATTGTGGCATAACTCAAAGTATGTCTCGCAAAGGAAACTG
TTATGACAATGCCGTTATTGAAAATTTCTTCGGTATCATGAAATCGGAATTTCTGTATCGAAAAGAATTTGAAAGTATAACACATTTCAAACAAGAATTA
GCAAAGTATATAGAATACTATAATCATAAAAGAATTAAGGCAAAATTAAAGGGTATGAGCCCAGTACAATACCGGGCTCATACCCTAGAGGCTGCCTAAT
GAAACGACCTGTCTAACTTTCGGGGGTCACTTCA
TTTAGATGGTACAGAAAGTGGAAAAACAATTGCTAAATCTATAGGAGTTAACCCTAGCGTACTTCGTGAGTGGATTAGACGATATGAGTCTTCAGGTGAA
AAGGCCTTTGAAAAGTGCTATACATTCTACCCAGCCCAGTATAAACTAGATGTACTTTATTATATGAATGAACACGGGACATCTATCAGAGAAACAGCGG
CACTTTTCAATATACCGTCTTATGAAACACTTCGGAAATGGAAAATAGCTTATGAAACAGGAGGATTGGATGCCCTACAATCAAAGAAAAAGGGGCGTCC
AACCATGAAAGATAAAAAAATAAAGCCAGTAGATGAAGGATCAATCGAAGCCTTACAAGCGGAAAATGAGCGTTTACGTATGGAAAATGCATATTTAAAA
AAGTTGAATGCCTTAGTTCAAAACAAAGAAAAATCACCAAACAAGACAAAGCGCAAGTAATCTACGAGTTAAGGCATGAATTTCCTATCAAGGAGTTACT
TCAACTCGCAAACATTCCACGTAGTACGTACTATTACTGGATGAAACGTTTTAATCGTCCTGATCCAAATGCGGAGGTAAAAGAACTGATTCAAGCTATT
TATAATGAACACGATGGGTGTTATGGTTATCGTCGTATTCGTGATGAACTTATGAATCGTGGACACAAAGTAAATCATAAAAAAGTGTATCGCCTTATGA
AAGAATTAGGGTTAAAATGTCTGGTTCGTATGAAAAAATACCGCTCTTACAAAGGGACAGTTGGGAAAATTGCGCCAAATATTTTAAATCGCAACTTCCA
AGCTGAAAAACCAAATGAAAAGTGGGTTACAGATATTACGGAGTTTAAGTTATTTGGGGAGAAGTTATACCTATCACCAATGTTGGATTTATTTAATGGT
GAAATTATCACGTATACAATTGGTTCAAGACCGACCTATTCCCTTGTTTCAACGATGTTGGACCAAGCCCTTTATTGTGTAACCAATCCGGATAAACTCC
TCATTCACTCTGATCAAGGTTGGCACTATCAAATGAAACAATACCGTCATTCTCTTAAGAATTGTGGCATAACTCAAAGTATGTCTCGCAAAGGAAACTG
TTATGACAATGCCGTTATTGAAAATTTCTTCGGTATCATGAAATCGGAATTTCTGTATCGAAAAGAATTTGAAAGTATAACACATTTCAAACAAGAATTA
GCAAAGTATATAGAATACTATAATCATAAAAGAATTAAGGCAAAATTAAAGGGTATGAGCCCAGTACAATACCGGGCTCATACCCTAGAGGCTGCCTAAT
GAAACGACCTGTCTAACTTTCGGGGGTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 507 bp | 168 aa | 54 | 560 | + | No |
Description : First part of the transposase
ORF sequence :
MAKFSSKEKIQAVKRYLDGTESGKTIAKSIGVNPSVLREWIRRYESSGEKAFEKCYTFYPAQYKLDVLYYMNEHGTSIRETAALFNIPSYETLRKWKIAY
ETGGLDALQSKKKGRPTMKDKKIKPVDEGSIEALQAENERLRMENAYLKKLNALVQNKEKSPNKTKRK
ETGGLDALQSKKKGRPTMKDKKIKPVDEGSIEALQAENERLRMENAYLKKLNALVQNKEKSPNKTKRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 930 bp | 309 aa | 470 | 1399 | + | No |
Description : Second part of the transposase
ORF sequence :
AFTYGKCIFKKVECLSSKQRKITKQDKAQVIYELRHEFPIKELLQLANIPRSTYYYWMKRFNRPDPNAEVKELIQAIYNEHDGCYGYRRIRDELMNRGHK
VNHKKVYRLMKELGLKCLVRMKKYRSYKGTVGKIAPNILNRNFQAEKPNEKWVTDITEFKLFGEKLYLSPMLDLFNGEIITYTIGSRPTYSLVSTMLDQA
LYCVTNPDKLLIHSDQGWHYQMKQYRHSLKNCGITQSMSRKGNCYDNAVIENFFGIMKSEFLYRKEFESITHFKQELAKYIEYYNHKRIKAKLKGMSPVQ
YRAHTLEAA
VNHKKVYRLMKELGLKCLVRMKKYRSYKGTVGKIAPNILNRNFQAEKPNEKWVTDITEFKLFGEKLYLSPMLDLFNGEIITYTIGSRPTYSLVSTMLDQA
LYCVTNPDKLLIHSDQGWHYQMKQYRHSLKNCGITQSMSRKGNCYDNAVIENFFGIMKSEFLYRKEFESITHFKQELAKYIEYYNHKRIKAKLKGMSPVQ
YRAHTLEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1346 bp | 448 aa | 54 | 1399 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKFSSKEKIQAVKRYLDGTESGKTIAKSIGVNPSVLREWIRRYESSGEKAFEKCYTFYPAQYKLDVLYYMNEHGTSIRETAALFNIPSYETLRKWKIAY
ETGGLDALQSKKKGRPTMKDKKIKPVDEGSIEALQAENERLRMENAYLKKVECLSSKQRKITKQDKAQVIYELRHEFPIKELLQLANIPRSTYYYWMKRF
NRPDPNAEVKELIQAIYNEHDGCYGYRRIRDELMNRGHKVNHKKVYRLMKELGLKCLVRMKKYRSYKGTVGKIAPNILNRNFQAEKPNEKWVTDITEFKL
FGEKLYLSPMLDLFNGEIITYTIGSRPTYSLVSTMLDQALYCVTNPDKLLIHSDQGWHYQMKQYRHSLKNCGITQSMSRKGNCYDNAVIENFFGIMKSEF
LYRKEFESITHFKQELAKYIEYYNHKRIKAKLKGMSPVQYRAHTLEAA
ETGGLDALQSKKKGRPTMKDKKIKPVDEGSIEALQAENERLRMENAYLKKVECLSSKQRKITKQDKAQVIYELRHEFPIKELLQLANIPRSTYYYWMKRF
NRPDPNAEVKELIQAIYNEHDGCYGYRRIRDELMNRGHKVNHKKVYRLMKELGLKCLVRMKKYRSYKGTVGKIAPNILNRNFQAEKPNEKWVTDITEFKL
FGEKLYLSPMLDLFNGEIITYTIGSRPTYSLVSTMLDQALYCVTNPDKLLIHSDQGWHYQMKQYRHSLKNCGITQSMSRKGNCYDNAVIENFFGIMKSEF
LYRKEFESITHFKQELAKYIEYYNHKRIKAKLKGMSPVQYRAHTLEAA
Blast result :
Comments
Genome in progress.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. ISBth10 is 80% aa similar to ISBth167.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift. ISBth10 is 80% aa similar to ISBth167.
References
1] Ziniu Y. & Ning Q. (2009) Direct submission to ISfinder.