ISBth8

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Bacillus thuringiensis
Bacillus thuringiensis subsp. kurstaki YBT-1520
DNA section
IS Length : 1288 bp

Ends


IR Length : 29/39

IRL : TGGTATTATCCCCTATAGGTAGACAGTAAAAAAAGAGTCCATCTGATATG
IRR : TGGTCATCTCCCTGTCAAGTAGACAGAAATAAAAAGAGTATTATGCCACG

Insertion site


Left flankDirect repeatRight flankDR Length
GCATCTGAACGCTTTACTCTTTT3

DNA sequence

TGGTATTATCCCCTATAGGTAGACAGTAAAAAAAGAGTCCATCTGATATGATGGACTCAATACTGTTTTATCTGGAGGGGATTTTTTTATGGCAAGAAAA
GGACAATCATTTCAAAAATATACAGAAGAACTAAAGCGAGAAGCAGTTCGTCTTCGATTAGAAGAGAGAAAATCATTGCGAGAAATTCGTGAACAACTCG
GTGTGAAGAGCGATGCCCAGATTATTGAGTGGGTAAAACGTGCGCAACAAGGGGAGTCCTTTGATGATCAACGTGGTGTCTGGAATCGAAAGAATTTTAA
TAGTCTAGAAGAAGAAAATGCTTATTTGAAGGCACAGGTTGAGTATTTAAAAAAGCGCAATCCAAATCTACACGGGAAGGAATGGTCCTAAAGAAAGAAC
GATTTTCTCTTATCGATTCATTACGCCAAGCCTATCCACTCCGATGGTTGCTTCAAATCGGGGAAGTTTCTAAGGCTGGCTACTATAAATGGCGGAAATA
TCATAACGTGCAGCGTTTACGTCAGAAAAGGGATATGTGGCATAAAGAACATATTTTATCTATTCATCGTCAGCACCCTTACTATGGGTATAAACGTATG
ACTCGTGCTTTGGCTCGAGAAGGAATGGTAATGAATCATAAACGTGTTCGTCGTCTTATGAGAGAGCTGGGTATTCAATCTATCATTCGAAAAAAACGTC
CGTTTTATGGAAGAAAAACATCTGTGGTATTTAAAAATCATTTGAATCGAGAGTTTCAAGCTGAAAAGCAAAATCAAAAATTCGTAACAGATATTACTTA
TGTACGAATCGGAGAACAATTTGCCTATTTGTCGGCTGTGTTAGATTTATACAACAATGAAATTGTTGCCTGGAAGCTATCTTCAAGAAATGATTTAGAT
TTGGTTTTAACTACATTACGTCAATTAGAAGGGAAGTCATTAACTACTAAGCCCCTTTTCCATTCAGATCAAGGGTTTCAATATACATCTAAATCCTACG
CAAAACAGTTAGAGAAATTAGGATTTGTCGGTAGTCATTCTAGACGTGGAAACTGTTTTGATAATGCGTGTATCGAATCATTCTTCTCACATTTAAAAAC
AGAGAAGTTGTATTTAGTGTGCCCAAAAACATATGAAATGGCCTACCGAGCAATTCAAGAATATATTCAGTTTTATAATACAGAAAGATTCCAAGAAAAA
CTTCACGGCCTTTCCCCGATAGAATATCGGGAAAAGGCCGTGGCATAATACTCTTTTTATTTCTGTCTACTTGACAGGGAGATGACCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
303 bp100 aa89391+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MARKGQSFQKYTEELKREAVRLRLEERKSLREIREQLGVKSDAQIIEWVKRAQQGESFDDQRGVWNRKNFNSLEEENAYLKAQVEYLKKRNPNLHGKEWS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
906 bp301 aa3431248+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

VFKKAQSKSTREGMVLKKERFSLIDSLRQAYPLRWLLQIGEVSKAGYYKWRKYHNVQRLRQKRDMWHKEHILSIHRQHPYYGYKRMTRALAREGMVMNHK
RVRRLMRELGIQSIIRKKRPFYGRKTSVVFKNHLNREFQAEKQNQKFVTDITYVRIGEQFAYLSAVLDLYNNEIVAWKLSSRNDLDLVLTTLRQLEGKSL
TTKPLFHSDQGFQYTSKSYAKQLEKLGFVGSHSRRGNCFDNACIESFFSHLKTEKLYLVCPKTYEMAYRAIQEYIQFYNTERFQEKLHGLSPIEYREKAV
A

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1160 bp386 aa891248+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MARKGQSFQKYTEELKREAVRLRLEERKSLREIREQLGVKSDAQIIEWVKRAQQGESFDDQRGVWNRKNFNSLEEENAYLKAQVEYLKKAQSKSTREGMV
LKKERFSLIDSLRQAYPLRWLLQIGEVSKAGYYKWRKYHNVQRLRQKRDMWHKEHILSIHRQHPYYGYKRMTRALAREGMVMNHKRVRRLMRELGIQSII
RKKRPFYGRKTSVVFKNHLNREFQAEKQNQKFVTDITYVRIGEQFAYLSAVLDLYNNEIVAWKLSSRNDLDLVLTTLRQLEGKSLTTKPLFHSDQGFQYT
SKSYAKQLEKLGFVGSHSRRGNCFDNACIESFFSHLKTEKLYLVCPKTYEMAYRAIQEYIQFYNTERFQEKLHGLSPIEYREKAVA

 

Blast result :
Comments
Genome in progress.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISBth8 is 83% aa similar to ISLtaq1.
References
1] Ziniu Y. & Ning Q. (2009) Direct submission to ISfinder.