ISBce19
- Family IS3
- Group IS150
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_004722 | ND | Bacillus cereus | Bacillus cereus ATCC 14579 |
DNA section
IS Length : 1445 bp
Ends
IR Length : 22/29
IRL : TGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGGGTGCAGTTT
IRR : TGAAGTGACCCCTAAAAGTTAGACACGGTTATTTCATTAGGCAACTTGAT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
AAAAGAGTAC | GTA | TCGTACTCTT | 3 |
DNA sequence
TGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGGGTGCAGTTTTTCTATGGCTAAATTTACAGCTGATGAAAAAATACAAATCGTTCTACGTT
ATTTGAATGGAAATGAAAGTTATCGAGAAATGGGTAAATCGATTGGTATAAGTGACACAATTATTTTGAATTGGGTAAACCAATATAAACAGAATGGTAT
GGAAGCTTTTCTAAAACGATGTACAAATTACACACAACAATTTAAACTAGACGTACTAAACTTTATGATTGAAAACGGTATGTCCTTATTTGAGACGGCA
GCTATCTTTAACATTCCTGCCCCTTCAACGATTTCTGTTTGGAAAAAACAGCTCGAAACACAAGGAATTGATGCCCTTCAATCTAAGAAAAAGGGGCGTC
CATCCATGAAAAAAGATTCAAATAAACAAGTAAAACAAGCTTTAGCTGAAGGGTCAGTCGAAGCACTTGAAGCACGTATTCAACAGCTTGAAATGGAAAA
TGAGTACTTAAAAAAGTTGAATGCCTTAGTTCAAAACAAGGAAAAATCACGAAACAAGACAAGGCACAAGTAGTCTATGAATTAAGGCATAAATATTCGG
TGAAGGCACTCGTGGAGCTAGCTGGTATTCCACGGAGTACGTATTATGATTTAGTAAAGAAAATGAATCGTCCGGATCAAGATGCCGATTTGAAAGCTGA
AATTAAAGCAATTTATGAGGAAAATGAAGGTCTTTATGGCTACCGTCGCATTCGTGATGAGTTAACGAATCGTGATCAGAAAGTGAATCACAAGAAGGTT
CAGCGCATTATGAAAGAGCTTGGATTAAAGTGTGTTGTGCGTATGAGGAAATATAAATCCTATAAAGGGAAAGTCGGTAAAATTGCACCTAATATTTTAG
AGCGTAATTTTTATACAGATGCCCCGAATCAAAAATGGGTAACGGACATCACAGAGTTTAAATTGTTTGGAGAGAAACTGTATGTATCACCTGTATTAGA
CTTGTATAATGGTGAAATTATTACCTATACAATTGGTTCTAGACCGACGTATTCAATTGTTTCAGAGATGTTAGAGAAAGCATTAGAACGCTTACCTGAA
AATCACCAGCTACTAATGCATTCGGATCAAGGATGGCATTATCAAATGAGACAGTACGTCCGGACACTTGAATCAAGAGCTATCGTCCAGAGTATGTCTC
GAAAAGGCAACTGTTACGACAACGCAGTAATAGAAAATTTCTTTGGGATTATGAAGTCGGAGTTCCTCTACATAAAAGAATTTGAAAATGTAGAGCACTT
TAAAATAGAATTAGAAAAATATATAGATTATTATAATACGAAACGGATTAAGGCGAAATTAAAAATGAGCCCGGTACAATACCGGACTCACTTTTATCAA
GTTGCCTAATGAAATAACCGTGTCTAACTTTTAGGGGTCACTTCA
ATTTGAATGGAAATGAAAGTTATCGAGAAATGGGTAAATCGATTGGTATAAGTGACACAATTATTTTGAATTGGGTAAACCAATATAAACAGAATGGTAT
GGAAGCTTTTCTAAAACGATGTACAAATTACACACAACAATTTAAACTAGACGTACTAAACTTTATGATTGAAAACGGTATGTCCTTATTTGAGACGGCA
GCTATCTTTAACATTCCTGCCCCTTCAACGATTTCTGTTTGGAAAAAACAGCTCGAAACACAAGGAATTGATGCCCTTCAATCTAAGAAAAAGGGGCGTC
CATCCATGAAAAAAGATTCAAATAAACAAGTAAAACAAGCTTTAGCTGAAGGGTCAGTCGAAGCACTTGAAGCACGTATTCAACAGCTTGAAATGGAAAA
TGAGTACTTAAAAAAGTTGAATGCCTTAGTTCAAAACAAGGAAAAATCACGAAACAAGACAAGGCACAAGTAGTCTATGAATTAAGGCATAAATATTCGG
TGAAGGCACTCGTGGAGCTAGCTGGTATTCCACGGAGTACGTATTATGATTTAGTAAAGAAAATGAATCGTCCGGATCAAGATGCCGATTTGAAAGCTGA
AATTAAAGCAATTTATGAGGAAAATGAAGGTCTTTATGGCTACCGTCGCATTCGTGATGAGTTAACGAATCGTGATCAGAAAGTGAATCACAAGAAGGTT
CAGCGCATTATGAAAGAGCTTGGATTAAAGTGTGTTGTGCGTATGAGGAAATATAAATCCTATAAAGGGAAAGTCGGTAAAATTGCACCTAATATTTTAG
AGCGTAATTTTTATACAGATGCCCCGAATCAAAAATGGGTAACGGACATCACAGAGTTTAAATTGTTTGGAGAGAAACTGTATGTATCACCTGTATTAGA
CTTGTATAATGGTGAAATTATTACCTATACAATTGGTTCTAGACCGACGTATTCAATTGTTTCAGAGATGTTAGAGAAAGCATTAGAACGCTTACCTGAA
AATCACCAGCTACTAATGCATTCGGATCAAGGATGGCATTATCAAATGAGACAGTACGTCCGGACACTTGAATCAAGAGCTATCGTCCAGAGTATGTCTC
GAAAAGGCAACTGTTACGACAACGCAGTAATAGAAAATTTCTTTGGGATTATGAAGTCGGAGTTCCTCTACATAAAAGAATTTGAAAATGTAGAGCACTT
TAAAATAGAATTAGAAAAATATATAGATTATTATAATACGAAACGGATTAAGGCGAAATTAAAAATGAGCCCGGTACAATACCGGACTCACTTTTATCAA
GTTGCCTAATGAAATAACCGTGTCTAACTTTTAGGGGTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
519 bp | 172 aa | 55 | 573 | + | No |
Description : First part of the transposase
ORF sequence :
MAKFTADEKIQIVLRYLNGNESYREMGKSIGISDTIILNWVNQYKQNGMEAFLKRCTNYTQQFKLDVLNFMIENGMSLFETAAIFNIPAPSTISVWKKQL
ETQGIDALQSKKKGRPSMKKDSNKQVKQALAEGSVEALEARIQQLEMENEYLKKLNALVQNKEKSRNKTRHK
ETQGIDALQSKKKGRPSMKKDSNKQVKQALAEGSVEALEARIQQLEMENEYLKKLNALVQNKEKSRNKTRHK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
906 bp | 301 aa | 504 | 1409 | + | No |
Description : Second part of the transposase
ORF sequence :
VLKKVECLSSKQGKITKQDKAQVVYELRHKYSVKALVELAGIPRSTYYDLVKKMNRPDQDADLKAEIKAIYEENEGLYGYRRIRDELTNRDQKVNHKKVQ
RIMKELGLKCVVRMRKYKSYKGKVGKIAPNILERNFYTDAPNQKWVTDITEFKLFGEKLYVSPVLDLYNGEIITYTIGSRPTYSIVSEMLEKALERLPEN
HQLLMHSDQGWHYQMRQYVRTLESRAIVQSMSRKGNCYDNAVIENFFGIMKSEFLYIKEFENVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQV
A
RIMKELGLKCVVRMRKYKSYKGKVGKIAPNILERNFYTDAPNQKWVTDITEFKLFGEKLYVSPVLDLYNGEIITYTIGSRPTYSIVSEMLEKALERLPEN
HQLLMHSDQGWHYQMRQYVRTLESRAIVQSMSRKGNCYDNAVIENFFGIMKSEFLYIKEFENVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQV
A
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1355 bp | 451 aa | 55 | 1409 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKFTADEKIQIVLRYLNGNESYREMGKSIGISDTIILNWVNQYKQNGMEAFLKRCTNYTQQFKLDVLNFMIENGMSLFETAAIFNIPAPSTISVWKKQL
ETQGIDALQSKKKGRPSMKKDSNKQVKQALAEGSVEALEARIQQLEMENEYLKKVECLSSKQGKITKQDKAQVVYELRHKYSVKALVELAGIPRSTYYDL
VKKMNRPDQDADLKAEIKAIYEENEGLYGYRRIRDELTNRDQKVNHKKVQRIMKELGLKCVVRMRKYKSYKGKVGKIAPNILERNFYTDAPNQKWVTDIT
EFKLFGEKLYVSPVLDLYNGEIITYTIGSRPTYSIVSEMLEKALERLPENHQLLMHSDQGWHYQMRQYVRTLESRAIVQSMSRKGNCYDNAVIENFFGIM
KSEFLYIKEFENVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQVA
ETQGIDALQSKKKGRPSMKKDSNKQVKQALAEGSVEALEARIQQLEMENEYLKKVECLSSKQGKITKQDKAQVVYELRHKYSVKALVELAGIPRSTYYDL
VKKMNRPDQDADLKAEIKAIYEENEGLYGYRRIRDELTNRDQKVNHKKVQRIMKELGLKCVVRMRKYKSYKGKVGKIAPNILERNFYTDAPNQKWVTDIT
EFKLFGEKLYVSPVLDLYNGEIITYTIGSRPTYSIVSEMLEKALERLPENHQLLMHSDQGWHYQMRQYVRTLESRAIVQSMSRKGNCYDNAVIENFFGIM
KSEFLYIKEFENVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQVA
Blast result :
Comments
ISBce19 is 75% aa similar to ISBth167. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Ivanova,N., Sorokin,A., Anderson,I., Galleron,N., Candelon,B., Kapatral,V., Bhattacharyya,A., Reznik,G., Mikhailova,N., Lapidus,A., Chu,L., Mazur,M., Goltsman,E., Larsen,N., D'Souza,M., Walunas,T., Grechkin,Y., Pusch,G., Haselkorn,R., Fonstein,M., Ehrlich,S.D., Overbeek,R. and Kyrpides,N. (2003) Nature 423 (6935), 87-91
2] Ivanova,N., Sorokin,A., Anderson,I., Galleron,N., Candelon,B., Kapatral,V., Bhattacharyya,A., Reznik,G., Mikhailova,N., Lapidus,A., Chu,L., Mazur,M., Goltsman,E., Larsen,N., D'Souza,M., Walunas,T., Grechkin,Y., Pusch,G., Haselkorn,R., Fonstein,M., Ehrlich,S.D., Overbeek,R. and Kyrpides,N. (2003) Nature 423 (6935), 87-91