ISBce18

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_004722 ND Bacillus cereus
Bacillus cereus ATCC 14579
DNA section
IS Length : 1450 bp

Ends


IR Length : 22/29

IRL : TGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTGCAGTT
IRR : TGAAGTGACCCCTAAAAGTTAGACACGGTTATTTCATTAGGCAGCTTGAT

Insertion site


Left flankDirect repeatRight flankDR Length
CAATTATTAATTGCAGAATGGCT3
AAGCTGACTATAAGCAGTCAGCT3
AGCCAAGGAAAATATCCTTGGCTT4
AAAAGCCGTGCATCAGCACGGCT3

DNA sequence

TGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTGCAGTTTTTTGTATGACAAAATTTACGAAGGATTTAAAGTCAACGCTCGTTCAAGG
ATTTAATCCGAAAATAATTTCTCAAGCAGAATATGCAAAACAGATGCATATTACAAAGGCACAATTTCAATACTGGTTACGCTTATACGAACTCCATGGA
GAAGAAGGTTTACACGAGGTCTATACAAATTACACCGCAGAGTTTAAACTAGACGTACTAAATTTTATGGCTCAATCGGGTATATCGTTAATGGATACAG
CGGCTATATTCATGGTTCCTTCTTTTACAACGGTTTATCAATGGAAGAAGCAATTTGAAGTAGGTGGCTTAGATGCCCTTGAACCAAAGAAAAAGGGGCG
TCCATCCATGAAAAATAAAAACACAAAACATGATACTAAAAAGATTCCGGCAGAAGGTTCAGTTGAAGCGTTACAAGCTGAATTAGAACGCTTACGTATG
GAAAATGCGTATTTAAAAAAGTTGAATGCCTTAGTTCAAAACAAGGAAAAATCACCAAACAAGACAAGGCACAAGTAGTCTATGAATTAAGGCATGAGTT
TCAAGTGAAAGAATTAGTAAAGTTAGCGGATATCCCTCGAAGCACCTATTATTTTTATGTAAAACAAATGGATAGAATCGATCCAGATGCCGATTTAAAA
GTAGAAATTAAAGCGATTTATGATGAACACGAAGGTCGTTATGGATATCGTCGTATTCGTAATGAGTTAGCCAATCGTAATCAAATAGTGAATCATAAAA
AAGTGCAACGAATTATGAAAGAGTTAGGTTTAAAATGTCTTGTACGTATGAAAAAATATAAATCTTATAAAGGAACAGTTGGTAAAATTGCACCTCATAT
TTTAGAGCGTAAATTTACGGCAGATACGCCAAATGAAAAATGGGTAACCGATATTACAGAATTTAAATTATTCGGTGAGAAACTTTATTTATCACCTGTA
TTAGACTTGTATAATGGTGAAATCATTACCTATACAATTGGCTCTAGACCGACGTATTCACTTGTTTCAGAAATGTTAGAGACAGCATTAGAAGGTTTAC
CTGAAAATCACCAACTAATGATGCATTCAGATCAAGGGTGGCATTATCAAATGAAACAATACCGTCACGCATTACAAAAAAGAGGTATCGTACAAAGTAT
GTCTCGTAAAGGCAACTGTTATGACAACGCAGTAATGGAGAATTTCTTTGGAATCATGAAGTCTGAGTTTCTCTACATAAAAGAGTTTGAAAGTGTAGAG
CACTTCAAAATAGAATTAGAAAAATATATAGATTATTACAATACAAAACGGATAAAGGCAAAATTAAAAATGAGCCCGGTGCAATACCGGACTCATTTTT
ATCAAGCTGCCTAATGAAATAACCGTGTCTAACTTTTAGGGGTCACTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
522 bp173 aa57578+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKFTKDLKSTLVQGFNPKIISQAEYAKQMHITKAQFQYWLRLYELHGEEGLHEVYTNYTAEFKLDVLNFMAQSGISLMDTAAIFMVPSFTTVYQWKKQF
EVGGLDALEPKKKGRPSMKNKNTKHDTKKIPAEGSVEALQAELERLRMENAYLKKLNALVQNKEKSPNKTRHK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
933 bp310 aa4821414+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

IRTLTYGKCVFKKVECLSSKQGKITKQDKAQVVYELRHEFDVKELVKLADIPRSTYYFYVKQMDRIDPDADLKVEIKAIYDEHEGRYGYRRIRNELANRN
QIVNHKKVQRIMKELGLKCLVRMKKYKSYKGTVGKIAPHILERKFTADTPNEKWVTDITEFKLFGEKLYLSPVLDLYNGEIITYTIGSRPTYSLVSEMLE
TALEGLPENHQLMMHSDQGWHYQMKQYRHALQKRGIVQSMSRKGNCYDNAVMENFFGIMKSEFLYIKEFESVEHFKIELEKYIDYYNTKRIKAKLKMSPV
QYRTHFYQAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1358 bp452 aa571414+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKFTKDLKSTLVQGFNPKIISQAEYAKQMHITKAQFQYWLRLYELHGEEGLHEVYTNYTAEFKLDVLNFMAQSGISLMDTAAIFMVPSFTTVYQWKKQF
EVGGLDALEPKKKGRPSMKNKNTKHDTKKIPAEGSVEALQAELERLRMENAYLKKVECLSSKQGKITKQDKAQVVYELRHEFDVKELVKLADIPRSTYYF
YVKQMDRIDPDADLKVEIKAIYDEHEGRYGYRRIRNELANRNQIVNHKKVQRIMKELGLKCLVRMKKYKSYKGTVGKIAPHILERKFTADTPNEKWVTDI
TEFKLFGEKLYLSPVLDLYNGEIITYTIGSRPTYSLVSEMLETALEGLPENHQLMMHSDQGWHYQMKQYRHALQKRGIVQSMSRKGNCYDNAVMENFFGI
MKSEFLYIKEFESVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQAA

 

Blast result :
Comments
ISBce18 is 71% aa similar to ISBth167. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Ivanova,N., Sorokin,A., Anderson,I., Galleron,N., Candelon,B., Kapatral,V., Bhattacharyya,A., Reznik,G., Mikhailova,N., Lapidus,A., Chu,L., Mazur,M., Goltsman,E., Larsen,N., D'Souza,M., Walunas,T., Grechkin,Y., Pusch,G., Haselkorn,R., Fonstein,M., Ehrlich,S.D., Overbeek,R. and Kyrpides,N. (2003) Nature 423 (6935), 87-91