ISBce18
- Family IS3
- Group IS150
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_004722 | ND | Bacillus cereus | Bacillus cereus ATCC 14579 |
DNA section
IS Length : 1450 bp
Ends
IR Length : 22/29
IRL : TGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTGCAGTT
IRR : TGAAGTGACCCCTAAAAGTTAGACACGGTTATTTCATTAGGCAGCTTGAT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CAATTATTAA | TTG | CAGAATGGCT | 3 |
AAGCTGACTA | TAA | GCAGTCAGCT | 3 |
AGCCAAGGAA | AATA | TCCTTGGCTT | 4 |
AAAAGCCGTG | CAT | CAGCACGGCT | 3 |
DNA sequence
TGAACTGCACCCCAATTGTTAGACACAGTCTAACAATTGGAGGTGCAGTTTTTTGTATGACAAAATTTACGAAGGATTTAAAGTCAACGCTCGTTCAAGG
ATTTAATCCGAAAATAATTTCTCAAGCAGAATATGCAAAACAGATGCATATTACAAAGGCACAATTTCAATACTGGTTACGCTTATACGAACTCCATGGA
GAAGAAGGTTTACACGAGGTCTATACAAATTACACCGCAGAGTTTAAACTAGACGTACTAAATTTTATGGCTCAATCGGGTATATCGTTAATGGATACAG
CGGCTATATTCATGGTTCCTTCTTTTACAACGGTTTATCAATGGAAGAAGCAATTTGAAGTAGGTGGCTTAGATGCCCTTGAACCAAAGAAAAAGGGGCG
TCCATCCATGAAAAATAAAAACACAAAACATGATACTAAAAAGATTCCGGCAGAAGGTTCAGTTGAAGCGTTACAAGCTGAATTAGAACGCTTACGTATG
GAAAATGCGTATTTAAAAAAGTTGAATGCCTTAGTTCAAAACAAGGAAAAATCACCAAACAAGACAAGGCACAAGTAGTCTATGAATTAAGGCATGAGTT
TCAAGTGAAAGAATTAGTAAAGTTAGCGGATATCCCTCGAAGCACCTATTATTTTTATGTAAAACAAATGGATAGAATCGATCCAGATGCCGATTTAAAA
GTAGAAATTAAAGCGATTTATGATGAACACGAAGGTCGTTATGGATATCGTCGTATTCGTAATGAGTTAGCCAATCGTAATCAAATAGTGAATCATAAAA
AAGTGCAACGAATTATGAAAGAGTTAGGTTTAAAATGTCTTGTACGTATGAAAAAATATAAATCTTATAAAGGAACAGTTGGTAAAATTGCACCTCATAT
TTTAGAGCGTAAATTTACGGCAGATACGCCAAATGAAAAATGGGTAACCGATATTACAGAATTTAAATTATTCGGTGAGAAACTTTATTTATCACCTGTA
TTAGACTTGTATAATGGTGAAATCATTACCTATACAATTGGCTCTAGACCGACGTATTCACTTGTTTCAGAAATGTTAGAGACAGCATTAGAAGGTTTAC
CTGAAAATCACCAACTAATGATGCATTCAGATCAAGGGTGGCATTATCAAATGAAACAATACCGTCACGCATTACAAAAAAGAGGTATCGTACAAAGTAT
GTCTCGTAAAGGCAACTGTTATGACAACGCAGTAATGGAGAATTTCTTTGGAATCATGAAGTCTGAGTTTCTCTACATAAAAGAGTTTGAAAGTGTAGAG
CACTTCAAAATAGAATTAGAAAAATATATAGATTATTACAATACAAAACGGATAAAGGCAAAATTAAAAATGAGCCCGGTGCAATACCGGACTCATTTTT
ATCAAGCTGCCTAATGAAATAACCGTGTCTAACTTTTAGGGGTCACTTCA
ATTTAATCCGAAAATAATTTCTCAAGCAGAATATGCAAAACAGATGCATATTACAAAGGCACAATTTCAATACTGGTTACGCTTATACGAACTCCATGGA
GAAGAAGGTTTACACGAGGTCTATACAAATTACACCGCAGAGTTTAAACTAGACGTACTAAATTTTATGGCTCAATCGGGTATATCGTTAATGGATACAG
CGGCTATATTCATGGTTCCTTCTTTTACAACGGTTTATCAATGGAAGAAGCAATTTGAAGTAGGTGGCTTAGATGCCCTTGAACCAAAGAAAAAGGGGCG
TCCATCCATGAAAAATAAAAACACAAAACATGATACTAAAAAGATTCCGGCAGAAGGTTCAGTTGAAGCGTTACAAGCTGAATTAGAACGCTTACGTATG
GAAAATGCGTATTTAAAAAAGTTGAATGCCTTAGTTCAAAACAAGGAAAAATCACCAAACAAGACAAGGCACAAGTAGTCTATGAATTAAGGCATGAGTT
TCAAGTGAAAGAATTAGTAAAGTTAGCGGATATCCCTCGAAGCACCTATTATTTTTATGTAAAACAAATGGATAGAATCGATCCAGATGCCGATTTAAAA
GTAGAAATTAAAGCGATTTATGATGAACACGAAGGTCGTTATGGATATCGTCGTATTCGTAATGAGTTAGCCAATCGTAATCAAATAGTGAATCATAAAA
AAGTGCAACGAATTATGAAAGAGTTAGGTTTAAAATGTCTTGTACGTATGAAAAAATATAAATCTTATAAAGGAACAGTTGGTAAAATTGCACCTCATAT
TTTAGAGCGTAAATTTACGGCAGATACGCCAAATGAAAAATGGGTAACCGATATTACAGAATTTAAATTATTCGGTGAGAAACTTTATTTATCACCTGTA
TTAGACTTGTATAATGGTGAAATCATTACCTATACAATTGGCTCTAGACCGACGTATTCACTTGTTTCAGAAATGTTAGAGACAGCATTAGAAGGTTTAC
CTGAAAATCACCAACTAATGATGCATTCAGATCAAGGGTGGCATTATCAAATGAAACAATACCGTCACGCATTACAAAAAAGAGGTATCGTACAAAGTAT
GTCTCGTAAAGGCAACTGTTATGACAACGCAGTAATGGAGAATTTCTTTGGAATCATGAAGTCTGAGTTTCTCTACATAAAAGAGTTTGAAAGTGTAGAG
CACTTCAAAATAGAATTAGAAAAATATATAGATTATTACAATACAAAACGGATAAAGGCAAAATTAAAAATGAGCCCGGTGCAATACCGGACTCATTTTT
ATCAAGCTGCCTAATGAAATAACCGTGTCTAACTTTTAGGGGTCACTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
522 bp | 173 aa | 57 | 578 | + | No |
Description : First part of the transposase
ORF sequence :
MTKFTKDLKSTLVQGFNPKIISQAEYAKQMHITKAQFQYWLRLYELHGEEGLHEVYTNYTAEFKLDVLNFMAQSGISLMDTAAIFMVPSFTTVYQWKKQF
EVGGLDALEPKKKGRPSMKNKNTKHDTKKIPAEGSVEALQAELERLRMENAYLKKLNALVQNKEKSPNKTRHK
EVGGLDALEPKKKGRPSMKNKNTKHDTKKIPAEGSVEALQAELERLRMENAYLKKLNALVQNKEKSPNKTRHK
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
933 bp | 310 aa | 482 | 1414 | + | No |
Description : Second part of the transposase
ORF sequence :
IRTLTYGKCVFKKVECLSSKQGKITKQDKAQVVYELRHEFDVKELVKLADIPRSTYYFYVKQMDRIDPDADLKVEIKAIYDEHEGRYGYRRIRNELANRN
QIVNHKKVQRIMKELGLKCLVRMKKYKSYKGTVGKIAPHILERKFTADTPNEKWVTDITEFKLFGEKLYLSPVLDLYNGEIITYTIGSRPTYSLVSEMLE
TALEGLPENHQLMMHSDQGWHYQMKQYRHALQKRGIVQSMSRKGNCYDNAVMENFFGIMKSEFLYIKEFESVEHFKIELEKYIDYYNTKRIKAKLKMSPV
QYRTHFYQAA
QIVNHKKVQRIMKELGLKCLVRMKKYKSYKGTVGKIAPHILERKFTADTPNEKWVTDITEFKLFGEKLYLSPVLDLYNGEIITYTIGSRPTYSLVSEMLE
TALEGLPENHQLMMHSDQGWHYQMKQYRHALQKRGIVQSMSRKGNCYDNAVMENFFGIMKSEFLYIKEFESVEHFKIELEKYIDYYNTKRIKAKLKMSPV
QYRTHFYQAA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1358 bp | 452 aa | 57 | 1414 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKFTKDLKSTLVQGFNPKIISQAEYAKQMHITKAQFQYWLRLYELHGEEGLHEVYTNYTAEFKLDVLNFMAQSGISLMDTAAIFMVPSFTTVYQWKKQF
EVGGLDALEPKKKGRPSMKNKNTKHDTKKIPAEGSVEALQAELERLRMENAYLKKVECLSSKQGKITKQDKAQVVYELRHEFDVKELVKLADIPRSTYYF
YVKQMDRIDPDADLKVEIKAIYDEHEGRYGYRRIRNELANRNQIVNHKKVQRIMKELGLKCLVRMKKYKSYKGTVGKIAPHILERKFTADTPNEKWVTDI
TEFKLFGEKLYLSPVLDLYNGEIITYTIGSRPTYSLVSEMLETALEGLPENHQLMMHSDQGWHYQMKQYRHALQKRGIVQSMSRKGNCYDNAVMENFFGI
MKSEFLYIKEFESVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQAA
EVGGLDALEPKKKGRPSMKNKNTKHDTKKIPAEGSVEALQAELERLRMENAYLKKVECLSSKQGKITKQDKAQVVYELRHEFDVKELVKLADIPRSTYYF
YVKQMDRIDPDADLKVEIKAIYDEHEGRYGYRRIRNELANRNQIVNHKKVQRIMKELGLKCLVRMKKYKSYKGTVGKIAPHILERKFTADTPNEKWVTDI
TEFKLFGEKLYLSPVLDLYNGEIITYTIGSRPTYSLVSEMLETALEGLPENHQLMMHSDQGWHYQMKQYRHALQKRGIVQSMSRKGNCYDNAVMENFFGI
MKSEFLYIKEFESVEHFKIELEKYIDYYNTKRIKAKLKMSPVQYRTHFYQAA
Blast result :
Comments
ISBce18 is 71% aa similar to ISBth167. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)
2] Ivanova,N., Sorokin,A., Anderson,I., Galleron,N., Candelon,B., Kapatral,V., Bhattacharyya,A., Reznik,G., Mikhailova,N., Lapidus,A., Chu,L., Mazur,M., Goltsman,E., Larsen,N., D'Souza,M., Walunas,T., Grechkin,Y., Pusch,G., Haselkorn,R., Fonstein,M., Ehrlich,S.D., Overbeek,R. and Kyrpides,N. (2003) Nature 423 (6935), 87-91
2] Ivanova,N., Sorokin,A., Anderson,I., Galleron,N., Candelon,B., Kapatral,V., Bhattacharyya,A., Reznik,G., Mikhailova,N., Lapidus,A., Chu,L., Mazur,M., Goltsman,E., Larsen,N., D'Souza,M., Walunas,T., Grechkin,Y., Pusch,G., Haselkorn,R., Fonstein,M., Ehrlich,S.D., Overbeek,R. and Kyrpides,N. (2003) Nature 423 (6935), 87-91