ISPfr13
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Propionibacterium freudenreichii | Propionibacterium freudenreichii |
DNA section
IS Length : 1383 bp
Ends
IR Length : 24/30
IRL : TGAACCGTCCCAGGTTTGGTGCCGCATCTTTTCGAGTTGAGAGGATGGCG
IRR : TGAACCGTCCCAGGTTCTCGGCCGCTTCTATGCGGGGAGCGGCTCTGGGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTGACGCCGATACGAACTCT | TGGGCCTGGGGGTGGAAGGG | 0 |
DNA sequence
TGAACCGTCCCAGGTTTGGTGCCGCATCTTTTCGAGTTGAGAGGATGGCGTCATGCCGAAGAAGATTGACCCCGCTCTGCGTGAGCGGGCCGTGAGGCTG
GTGCTGGAGCACCGGTCAGAGTACCCGTCGAACGCGAAGGTGATCGCGGCCGTAGCCCGGCAGGAGTGCGTCGGTGCGGAGTCCTTGCGACGGTGGGTGA
TCCAGGCCGAGATCGACGCGGGTGACCGTGGTGGTCAGACCAGCGAAGAGCATGCGGAGATCCGCCGGCTCAAGGCCGAGAACCGGGCGTTGCGTGAGGA
CGTCGCGATCTTGAAGGCGGCGACGGCTTTCTTCGTGGGGGAACTCGACCCCCGCAACCGTTGATGATGGGCTTCATCGACCAGATGCGCTCGGAGGGCT
TTGCCGTCGAGTCGATCATTCGCGTCCTGCGTGAGCAGGGCGTGAAGATCGCGGCACGCACCTACCGGGCCTGGAAGCGAGGCCGGGTCGCGGTCCGGAC
CGTGACCGACGCGATCGTCGTGGACGCGGTCCGTGACGCGGCCTGGCGCGACGAGACACTGCCCGGCGGGAGCACACGCCGGAAGATGACCCCGGAGGGT
CTTTACGGACGGAAGAAGATGACCGCGTTCATCCGCCGCACCACGCTCCGGGACGTGTCTCGCGGTGCGGTGGACCGCGCGATGCGGGCCCTGGGCCTTT
CCGGGGTCACGCGGGCGAAAGCGATCCGCACCACGATCCCCGCCAAGGATGGGGTCCGTGCCGGGGACCTGCTGAACCGGGACTTCACCGCTCCGCGCCC
GGATCACACGTGGGTGACGGACTTCACGTATGTGCGGACGTGGGCCGGATGGGTGTATGTCGCGTTCATCCTCGACGTGTTCTCGCAACGCATCGTCGCC
TGGCATGCGCAGACCAGCAAGCACACGGACCTTGTGATGATCCCGCTGCGGATGGCGCTGTGGCGGCGTGACCGGGACGGGCATCCCGTCGGTCCCGGCC
AGCTCCGGCATCACTCCGACGCCGGATCCCAAGGCGGACCTGCCCAATACACATCCGTAGTGCTGACCGATCACCTCGCGCTCGAGCAGATCGCACCCTC
GATCGGAAGCATCGGCGACGCGTACGACAACGCCCTGATGGAGACGATCAACGGCGTCTACAAGGCCGAATGCGTCCGCACCACCATCTTCCACGACGGC
CCCTACAAGACGATCGCGGACGTCGAGTTCGCGACAGCTGGGTGGGTCGACTGGTACAACCACCGCAGGCTCCACGGTAGCCTCGGAATGGTCAGCCCCG
ACGAGTTCGAGGCGGCCTACTACGCGGCCCTCAACCCAGAGCCGCTCCCCGCATAGAAGCGGCCGAGAACCTGGGACGGTTCA
GTGCTGGAGCACCGGTCAGAGTACCCGTCGAACGCGAAGGTGATCGCGGCCGTAGCCCGGCAGGAGTGCGTCGGTGCGGAGTCCTTGCGACGGTGGGTGA
TCCAGGCCGAGATCGACGCGGGTGACCGTGGTGGTCAGACCAGCGAAGAGCATGCGGAGATCCGCCGGCTCAAGGCCGAGAACCGGGCGTTGCGTGAGGA
CGTCGCGATCTTGAAGGCGGCGACGGCTTTCTTCGTGGGGGAACTCGACCCCCGCAACCGTTGATGATGGGCTTCATCGACCAGATGCGCTCGGAGGGCT
TTGCCGTCGAGTCGATCATTCGCGTCCTGCGTGAGCAGGGCGTGAAGATCGCGGCACGCACCTACCGGGCCTGGAAGCGAGGCCGGGTCGCGGTCCGGAC
CGTGACCGACGCGATCGTCGTGGACGCGGTCCGTGACGCGGCCTGGCGCGACGAGACACTGCCCGGCGGGAGCACACGCCGGAAGATGACCCCGGAGGGT
CTTTACGGACGGAAGAAGATGACCGCGTTCATCCGCCGCACCACGCTCCGGGACGTGTCTCGCGGTGCGGTGGACCGCGCGATGCGGGCCCTGGGCCTTT
CCGGGGTCACGCGGGCGAAAGCGATCCGCACCACGATCCCCGCCAAGGATGGGGTCCGTGCCGGGGACCTGCTGAACCGGGACTTCACCGCTCCGCGCCC
GGATCACACGTGGGTGACGGACTTCACGTATGTGCGGACGTGGGCCGGATGGGTGTATGTCGCGTTCATCCTCGACGTGTTCTCGCAACGCATCGTCGCC
TGGCATGCGCAGACCAGCAAGCACACGGACCTTGTGATGATCCCGCTGCGGATGGCGCTGTGGCGGCGTGACCGGGACGGGCATCCCGTCGGTCCCGGCC
AGCTCCGGCATCACTCCGACGCCGGATCCCAAGGCGGACCTGCCCAATACACATCCGTAGTGCTGACCGATCACCTCGCGCTCGAGCAGATCGCACCCTC
GATCGGAAGCATCGGCGACGCGTACGACAACGCCCTGATGGAGACGATCAACGGCGTCTACAAGGCCGAATGCGTCCGCACCACCATCTTCCACGACGGC
CCCTACAAGACGATCGCGGACGTCGAGTTCGCGACAGCTGGGTGGGTCGACTGGTACAACCACCGCAGGCTCCACGGTAGCCTCGGAATGGTCAGCCCCG
ACGAGTTCGAGGCGGCCTACTACGCGGCCCTCAACCCAGAGCCGCTCCCCGCATAGAAGCGGCCGAGAACCTGGGACGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 312 bp | 103 aa | 53 | 364 | + | No |
Description : First part of the transposase
ORF sequence :
MPKKIDPALRERAVRLVLEHRSEYPSNAKVIAAVARQECVGAESLRRWVIQAEIDAGDRGGQTSEEHAEIRRLKAENRALREDVAILKAATAFFVGELDP
RNR
RNR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1059 bp | 352 aa | 298 | 1356 | + | No |
Description : Second part of the transposase
ORF sequence :
GRRDLEGGDGFLRGGTRPPQPLMMGFIDQMRSEGFAVESIIRVLREQGVKIAARTYRAWKRGRVAVRTVTDAIVVDAVRDAAWRDETLPGGSTRRKMTPE
GLYGRKKMTAFIRRTTLRDVSRGAVDRAMRALGLSGVTRAKAIRTTIPAKDGVRAGDLLNRDFTAPRPDHTWVTDFTYVRTWAGWVYVAFILDVFSQRIV
AWHAQTSKHTDLVMIPLRMALWRRDRDGHPVGPGQLRHHSDAGSQGGPAQYTSVVLTDHLALEQIAPSIGSIGDAYDNALMETINGVYKAECVRTTIFHD
GPYKTIADVEFATAGWVDWYNHRRLHGSLGMVSPDEFEAAYYAALNPEPLPA
GLYGRKKMTAFIRRTTLRDVSRGAVDRAMRALGLSGVTRAKAIRTTIPAKDGVRAGDLLNRDFTAPRPDHTWVTDFTYVRTWAGWVYVAFILDVFSQRIV
AWHAQTSKHTDLVMIPLRMALWRRDRDGHPVGPGQLRHHSDAGSQGGPAQYTSVVLTDHLALEQIAPSIGSIGDAYDNALMETINGVYKAECVRTTIFHD
GPYKTIADVEFATAGWVDWYNHRRLHGSLGMVSPDEFEAAYYAALNPEPLPA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1304 bp | 434 aa | 53 | 1356 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKKIDPALRERAVRLVLEHRSEYPSNAKVIAAVARQECVGAESLRRWVIQAEIDAGDRGGQTSEEHAEIRRLKAENRALREDVAILKAATAFLRGGTRP
PQPLMMGFIDQMRSEGFAVESIIRVLREQGVKIAARTYRAWKRGRVAVRTVTDAIVVDAVRDAAWRDETLPGGSTRRKMTPEGLYGRKKMTAFIRRTTLR
DVSRGAVDRAMRALGLSGVTRAKAIRTTIPAKDGVRAGDLLNRDFTAPRPDHTWVTDFTYVRTWAGWVYVAFILDVFSQRIVAWHAQTSKHTDLVMIPLR
MALWRRDRDGHPVGPGQLRHHSDAGSQGGPAQYTSVVLTDHLALEQIAPSIGSIGDAYDNALMETINGVYKAECVRTTIFHDGPYKTIADVEFATAGWVD
WYNHRRLHGSLGMVSPDEFEAAYYAALNPEPLPA
PQPLMMGFIDQMRSEGFAVESIIRVLREQGVKIAARTYRAWKRGRVAVRTVTDAIVVDAVRDAAWRDETLPGGSTRRKMTPEGLYGRKKMTAFIRRTTLR
DVSRGAVDRAMRALGLSGVTRAKAIRTTIPAKDGVRAGDLLNRDFTAPRPDHTWVTDFTYVRTWAGWVYVAFILDVFSQRIVAWHAQTSKHTDLVMIPLR
MALWRRDRDGHPVGPGQLRHHSDAGSQGGPAQYTSVVLTDHLALEQIAPSIGSIGDAYDNALMETINGVYKAECVRTTIFHDGPYKTIADVEFATAGWVD
WYNHRRLHGSLGMVSPDEFEAAYYAALNPEPLPA
Blast result :
Comments
ISPfr13 is 58% aa similar to ISMysp3. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
In the genome, this sequence was disrupted by insertion of ISPfr14.
In the genome, this sequence was disrupted by insertion of ISPfr14.
References
1] ISfinder annotation (2009)