ISPfr12
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Propionibacterium freudenreichii | Propionibacterium freudenreichii |
DNA section
IS Length : 1350 bp
Ends
IR Length : 26/28
IRL : TGAAGCGCCCTGAGTTTGTTGGAGACTCCTGACCTTGGAAACGAGGATGG
IRR : TGAAGCGCCCTGAGTCTGATGGAGACTCGCGCTATTTGATTCCGATCAGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCGTAGAGC | AAA | CGAGCACAGC | 3 |
| TGGTGCTTTT | GACATGGTCG | 0 | |
| AGAGGCGGAC | GCTGACGGGA | 0 | |
| CGCCCGGACG | CTG | CTGCGACAGG | 3 |
| GGTGGCTAGG | CCCATTTCGG | 0 | |
| GATCTGCCGG | TCT | ACAACGCCAA | 3 |
DNA sequence
TGAAGCGCCCTGAGTTTGTTGGAGACTCCTGACCTTGGAAACGAGGATGGGGTTATGCCGAAGGAACTGGCGAATGGGAAGCCGACGACGCGTCGCTACT
CGGTCGAGGAGAAGGCCGCCGCGGTGCGGATGGTGAGGACGCTGCGCGCGGAGCTGGGCGTCACGCAGGGGACGGTGCAGCGGGTCGCGACGCAGCTCGG
TTACGGGGTCGAGTCCGTGCGGATGTGGGTCAAGCAGGCCGACGTCGACGACGGTGTCGTCCCCGGTGTGAGCTCGGCGGAGGAGCAGCGGGTGAAAGAG
CTCGAGCAAGAGAATCGGGAGCTGCGCCGGGCCAACGAGGTGCTGAAACGGGCGGCGTCTTTCTTCGGGGCGGAGCTCGACCGCCACTACCGGAAGTAGT
CGCGTTCATCGACGCGAACAAGGACGACGTCGTGGACGGTCGCCGGCTCGGAGTCGAGCTCATCTGCAGACTGTTGCAGGTGGCTCCGAGCAGCTACTAC
GCCGCCAAGACCCGTGCGCCCTCTGCCCGTGCGCTGCGTGACGAGGAACTGATCCCGCAGCTGGTCGAGATCTGGGAGGCCAACTACCGCGTCTACGGGG
TCCGCAAGCTCTGGAAGGCTGCCCGGCGTGGGGGCATCATGATCGGCCGCGACCAGACTGCGAGGCTGATGCGCGTCGCAGGGATCGAAGGTGCGAGGCG
GTCGAAGCGGGTGAAGACCACGCGGCCGGACCCGGCGTCGTCGCGGCACCCGGACCTGGTCAAGCGGGAGTTCACCGCGACGGCACCGAACCGGCTCTGG
GTGACCGATCTGACATTCGTGCCCACCTGGGCTGGCGTCGCCTACGTCTGCTTCATCATCGACGCGTTCTCCCGGATGATCGTGGGGTGGCGGGTCGCGT
CACATATGCGCACCGAGATGGTCCTCGACGCGATCGAGATGGCGCGCTGGTCCCGAGGCGCCCACCATGAGGATCTGCGGTGTCACAGCGACGCGGGCTG
TCAATTCACATCGATTCGCTACGGCGAACGCCTCGCAGAGATCGGTGCGACACCCTCGATCGGGACCGTCGGCGATTCGTATGACAACGCCCTGGCCGAG
ACGGTGAACGGCTACTACAAGGCCGAGTTGGTTCGCGGGCCCACCCGCTCGGGACCGTGGAAGACGGTCGAGGATCTCGAGCTCGCGACGCTCGGCTGGG
TGCACTGGCACAACACGCAGCGCCTCCACGGATACCTCGGAGACGTCCCACCCGCCGAGTTCGAGAACGCGTTCTATGCTGTCCCCAACGACAGCAATCT
GCTGATCGGAATCAAATAGCGCGAGTCTCCATCAGACTCAGGGCGCTTCA
CGGTCGAGGAGAAGGCCGCCGCGGTGCGGATGGTGAGGACGCTGCGCGCGGAGCTGGGCGTCACGCAGGGGACGGTGCAGCGGGTCGCGACGCAGCTCGG
TTACGGGGTCGAGTCCGTGCGGATGTGGGTCAAGCAGGCCGACGTCGACGACGGTGTCGTCCCCGGTGTGAGCTCGGCGGAGGAGCAGCGGGTGAAAGAG
CTCGAGCAAGAGAATCGGGAGCTGCGCCGGGCCAACGAGGTGCTGAAACGGGCGGCGTCTTTCTTCGGGGCGGAGCTCGACCGCCACTACCGGAAGTAGT
CGCGTTCATCGACGCGAACAAGGACGACGTCGTGGACGGTCGCCGGCTCGGAGTCGAGCTCATCTGCAGACTGTTGCAGGTGGCTCCGAGCAGCTACTAC
GCCGCCAAGACCCGTGCGCCCTCTGCCCGTGCGCTGCGTGACGAGGAACTGATCCCGCAGCTGGTCGAGATCTGGGAGGCCAACTACCGCGTCTACGGGG
TCCGCAAGCTCTGGAAGGCTGCCCGGCGTGGGGGCATCATGATCGGCCGCGACCAGACTGCGAGGCTGATGCGCGTCGCAGGGATCGAAGGTGCGAGGCG
GTCGAAGCGGGTGAAGACCACGCGGCCGGACCCGGCGTCGTCGCGGCACCCGGACCTGGTCAAGCGGGAGTTCACCGCGACGGCACCGAACCGGCTCTGG
GTGACCGATCTGACATTCGTGCCCACCTGGGCTGGCGTCGCCTACGTCTGCTTCATCATCGACGCGTTCTCCCGGATGATCGTGGGGTGGCGGGTCGCGT
CACATATGCGCACCGAGATGGTCCTCGACGCGATCGAGATGGCGCGCTGGTCCCGAGGCGCCCACCATGAGGATCTGCGGTGTCACAGCGACGCGGGCTG
TCAATTCACATCGATTCGCTACGGCGAACGCCTCGCAGAGATCGGTGCGACACCCTCGATCGGGACCGTCGGCGATTCGTATGACAACGCCCTGGCCGAG
ACGGTGAACGGCTACTACAAGGCCGAGTTGGTTCGCGGGCCCACCCGCTCGGGACCGTGGAAGACGGTCGAGGATCTCGAGCTCGCGACGCTCGGCTGGG
TGCACTGGCACAACACGCAGCGCCTCCACGGATACCTCGGAGACGTCCCACCCGCCGAGTTCGAGAACGCGTTCTATGCTGTCCCCAACGACAGCAATCT
GCTGATCGGAATCAAATAGCGCGAGTCTCCATCAGACTCAGGGCGCTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 345 bp | 114 aa | 55 | 399 | + | No |
Description : First part of the transposase
ORF sequence :
MPKELANGKPTTRRYSVEEKAAAVRMVRTLRAELGVTQGTVQRVATQLGYGVESVRMWVKQADVDDGVVPGVSSAEEQRVKELEQENRELRRANEVLKRA
ASFFGAELDRHYRK
ASFFGAELDRHYRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1035 bp | 344 aa | 225 | 1259 | + | No |
Description : Second part of the transposase
ORF sequence :
VGQAGARARESGAAPGQRGAETGGVFLRGGARPPLPEVVAFIDANKDDVVDGRRLGVELICRLLQVAPSSYYAAKTRAPSARALRDEELIPQLVEIWEAN
YRVYGVRKLWKAARRGGIMIGRDQTARLMRVAGIEGARRSKRVKTTRPDPASSRHPDLVKREFTATAPNRLWVTDLTFVPTWAGVAYVCFIIDAFSRMIV
GWRVASHMRTEMVLDAIEMARWSRGAHHEDLRCHSDAGCQFTSIRYGERLAEIGATPSIGTVGDSYDNALAETVNGYYKAELVRGPTRSGPWKTVEDLEL
ATLGWVHWHNTQRLHGYLGDVPPAEFENAFYAVPNDSNLLIGIK
YRVYGVRKLWKAARRGGIMIGRDQTARLMRVAGIEGARRSKRVKTTRPDPASSRHPDLVKREFTATAPNRLWVTDLTFVPTWAGVAYVCFIIDAFSRMIV
GWRVASHMRTEMVLDAIEMARWSRGAHHEDLRCHSDAGCQFTSIRYGERLAEIGATPSIGTVGDSYDNALAETVNGYYKAELVRGPTRSGPWKTVEDLEL
ATLGWVHWHNTQRLHGYLGDVPPAEFENAFYAVPNDSNLLIGIK
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 422 aa | 55 | 1259 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKELANGKPTTRRYSVEEKAAAVRMVRTLRAELGVTQGTVQRVATQLGYGVESVRMWVKQADVDDGVVPGVSSAEEQRVKELEQENRELRRANEVLKRA
ASFFLRGGARPPLPEVVAFIDANKDDVVDGRRLGVELICRLLQVAPSSYYAAKTRAPSARALRDEELIPQLVEIWEANYRVYGVRKLWKAARRGGIMIGR
DQTARLMRVAGIEGARRSKRVKTTRPDPASSRHPDLVKREFTATAPNRLWVTDLTFVPTWAGVAYVCFIIDAFSRMIVGWRVASHMRTEMVLDAIEMARW
SRGAHHEDLRCHSDAGCQFTSIRYGERLAEIGATPSIGTVGDSYDNALAETVNGYYKAELVRGPTRSGPWKTVEDLELATLGWVHWHNTQRLHGYLGDVP
PAEFENAFYAVPNDSNLLIGIK
ASFFLRGGARPPLPEVVAFIDANKDDVVDGRRLGVELICRLLQVAPSSYYAAKTRAPSARALRDEELIPQLVEIWEANYRVYGVRKLWKAARRGGIMIGR
DQTARLMRVAGIEGARRSKRVKTTRPDPASSRHPDLVKREFTATAPNRLWVTDLTFVPTWAGVAYVCFIIDAFSRMIVGWRVASHMRTEMVLDAIEMARW
SRGAHHEDLRCHSDAGCQFTSIRYGERLAEIGATPSIGTVGDSYDNALAETVNGYYKAELVRGPTRSGPWKTVEDLELATLGWVHWHNTQRLHGYLGDVP
PAEFENAFYAVPNDSNLLIGIK
Blast result :
Comments
ISPfr12 is 84% aa similar to ISTesp1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)