ISPfr11
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Propionibacterium freudenreichii | Propionibacterium freudenreichii |
DNA section
IS Length : 1315 bp
Ends
IR Length : 17/24
IRL : TGGGCCTACCCTGGTTTTTCGGACACCTGATGTGGCGGGGCCTGGTGGCC
IRR : TGGGGTTACCCGCAAGTTTCGGACGGTTGGATTGGTTCAGGCGGCCCGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCCGTGCAG | ATTCAGCGTG | 0 | |
| GAGGATGGGG | TTAT | GCCGAAGGAA | 4 |
| TGCAACAAGG | GGC | AACTACCGGCG | 3 |
| TCCGGTGTCG | CGGG | TGATGCGCGT | 4 |
| AGAGGTCAAC | GGCAACTACC | 0 |
DNA sequence
TGGGCCTACCCTGGTTTTTCGGACACCTGATGTGGCGGGGCCTGGTGGCCCTGGGGGAAGGTGTTGTCATGAGCACGGGGAACGAGAAGACGCGGAGGCA
GCGTCGCAAGTTCACGGCACAGTACCGCCATGAGGCGGCGAGGATGGTGATCGACTCGGGTCGCACGATCGCCGAGGTCGCCCAGGAGCTGGGGCTGGGT
CCCCAACTGTTGGGCAGGTGGGTGAAGGCAGAGAAGGAGACCATGACGCCGAGCACGTTGAGTCCGGATGAACGCGAGGAGTTGAAGCGGCTCCGCAAGG
AGAACGCGGACCTGCGGATGGACAACGAGTTCTTGGGAAAAGCAGCGGCCTTCTTCGCTGCGAAGCATCAGTGAGTGAGAAGTACACGCTGATGCAGGCG
GAGAAGGCTCGGTACCCGATTGCCCGGATGGCCCGCCTGTTGAAAGTGTCCACTTCCGGGTACTACGCGTGGGTTGCGGCCCAGCAGCGGGATGGGGATC
ATCTTCTCCCCAGCCTCCGGGCGCGTCGGCGCCTGGATGAGGCGGTGCGCCGCATATGGGTGGACTCCCGAAGCACGTACGGGTACCTGCGTGTGTGCGC
CCAGCTTCGCCGTGAGGGGGTGGTGGTGGATCGTAAGACGGTGGCGGCCTCGATGCGCCGTCAGGGCCTTGCGGGCATCAGCCCGCGCCGGTTCCGTCCG
GTGACGACGATACCGGGGACGCGCACGCATTCGATCCCTGACCGGGTCAAGCGACACTGGGACACGGGGCAGGTGGATCGCGTGTGGGTCACCGACATCA
CCTACCTGAGGACGCGGGCCGGGTGGGTGTACCTGTGCGCCATCAAGGACGCTTGCTACCGGAAGGTCATCGCAACCGCCATGTCGACGACCATGACGAC
GGACCTCGTGGAGGAGGCGTTGCGCCGGGCTCGCATTCTTCGCCCGAACGCGCCCAGGAAAGTGATCATCCATTCCGACCGTGGCACGCAATTCACCTCC
GAGCAGATGTATGAGTGCTGCCGGGAACTCCACCTCGACCAGTCAATGGGACGCACCGGGGTGTGCTGGGACAATGCGATGATCGAATCCCAGTGGTCCG
TGTTGAAGGCTGAGTTCTACGACCGCTACGAGTGGGACACCCCCCAGCAGGCCATTCAGGGCGTGGAGGAGTGGATCTACGACTTCTACAACACGAAGCG
CCTCAACTCAGCGATCGGCTACCAGACCCCAGTCGAGTTCGAAGCCCAACACGCGGCAGCCCTGACACGGGCCGCCTGAACCAATCCAACCGTCCGAAAC
TTGCGGGTAACCCCA
GCGTCGCAAGTTCACGGCACAGTACCGCCATGAGGCGGCGAGGATGGTGATCGACTCGGGTCGCACGATCGCCGAGGTCGCCCAGGAGCTGGGGCTGGGT
CCCCAACTGTTGGGCAGGTGGGTGAAGGCAGAGAAGGAGACCATGACGCCGAGCACGTTGAGTCCGGATGAACGCGAGGAGTTGAAGCGGCTCCGCAAGG
AGAACGCGGACCTGCGGATGGACAACGAGTTCTTGGGAAAAGCAGCGGCCTTCTTCGCTGCGAAGCATCAGTGAGTGAGAAGTACACGCTGATGCAGGCG
GAGAAGGCTCGGTACCCGATTGCCCGGATGGCCCGCCTGTTGAAAGTGTCCACTTCCGGGTACTACGCGTGGGTTGCGGCCCAGCAGCGGGATGGGGATC
ATCTTCTCCCCAGCCTCCGGGCGCGTCGGCGCCTGGATGAGGCGGTGCGCCGCATATGGGTGGACTCCCGAAGCACGTACGGGTACCTGCGTGTGTGCGC
CCAGCTTCGCCGTGAGGGGGTGGTGGTGGATCGTAAGACGGTGGCGGCCTCGATGCGCCGTCAGGGCCTTGCGGGCATCAGCCCGCGCCGGTTCCGTCCG
GTGACGACGATACCGGGGACGCGCACGCATTCGATCCCTGACCGGGTCAAGCGACACTGGGACACGGGGCAGGTGGATCGCGTGTGGGTCACCGACATCA
CCTACCTGAGGACGCGGGCCGGGTGGGTGTACCTGTGCGCCATCAAGGACGCTTGCTACCGGAAGGTCATCGCAACCGCCATGTCGACGACCATGACGAC
GGACCTCGTGGAGGAGGCGTTGCGCCGGGCTCGCATTCTTCGCCCGAACGCGCCCAGGAAAGTGATCATCCATTCCGACCGTGGCACGCAATTCACCTCC
GAGCAGATGTATGAGTGCTGCCGGGAACTCCACCTCGACCAGTCAATGGGACGCACCGGGGTGTGCTGGGACAATGCGATGATCGAATCCCAGTGGTCCG
TGTTGAAGGCTGAGTTCTACGACCGCTACGAGTGGGACACCCCCCAGCAGGCCATTCAGGGCGTGGAGGAGTGGATCTACGACTTCTACAACACGAAGCG
CCTCAACTCAGCGATCGGCTACCAGACCCCAGTCGAGTTCGAAGCCCAACACGCGGCAGCCCTGACACGGGCCGCCTGAACCAATCCAACCGTCCGAAAC
TTGCGGGTAACCCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 306 bp | 101 aa | 69 | 374 | + | No |
Description : First part of the transposase
ORF sequence :
MSTGNEKTRRQRRKFTAQYRHEAARMVIDSGRTIAEVAQELGLGPQLLGRWVKAEKETMTPSTLSPDEREELKRLRKENADLRMDNEFLGKAAAFFAAKH
Q
Q
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1008 bp | 335 aa | 272 | 1279 | + | No |
Description : Second part of the transposase
ORF sequence :
TRGVEAAPQGERGPADGQRVLGKSSGLLRCEASVSEKYTLMQAEKARYPIARMARLLKVSTSGYYAWVAAQQRDGDHLLPSLRARRRLDEAVRRIWVDSR
STYGYLRVCAQLRREGVVVDRKTVAASMRRQGLAGISPRRFRPVTTIPGTRTHSIPDRVKRHWDTGQVDRVWVTDITYLRTRAGWVYLCAIKDACYRKVI
ATAMSTTMTTDLVEEALRRARILRPNAPRKVIIHSDRGTQFTSEQMYECCRELHLDQSMGRTGVCWDNAMIESQWSVLKAEFYDRYEWDTPQQAIQGVEE
WIYDFYNTKRLNSAIGYQTPVEFEAQHAAALTRAA
STYGYLRVCAQLRREGVVVDRKTVAASMRRQGLAGISPRRFRPVTTIPGTRTHSIPDRVKRHWDTGQVDRVWVTDITYLRTRAGWVYLCAIKDACYRKVI
ATAMSTTMTTDLVEEALRRARILRPNAPRKVIIHSDRGTQFTSEQMYECCRELHLDQSMGRTGVCWDNAMIESQWSVLKAEFYDRYEWDTPQQAIQGVEE
WIYDFYNTKRLNSAIGYQTPVEFEAQHAAALTRAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1211 bp | 403 aa | 69 | 1279 | + | Yes |
Chemistry : DDE
ORF sequence :
MSTGNEKTRRQRRKFTAQYRHEAARMVIDSGRTIAEVAQELGLGPQLLGRWVKAEKETMTPSTLSPDEREELKRLRKENADLRMDNEFLGKSSGLLRCEA
SVSEKYTLMQAEKARYPIARMARLLKVSTSGYYAWVAAQQRDGDHLLPSLRARRRLDEAVRRIWVDSRSTYGYLRVCAQLRREGVVVDRKTVAASMRRQG
LAGISPRRFRPVTTIPGTRTHSIPDRVKRHWDTGQVDRVWVTDITYLRTRAGWVYLCAIKDACYRKVIATAMSTTMTTDLVEEALRRARILRPNAPRKVI
IHSDRGTQFTSEQMYECCRELHLDQSMGRTGVCWDNAMIESQWSVLKAEFYDRYEWDTPQQAIQGVEEWIYDFYNTKRLNSAIGYQTPVEFEAQHAAALT
RAA
SVSEKYTLMQAEKARYPIARMARLLKVSTSGYYAWVAAQQRDGDHLLPSLRARRRLDEAVRRIWVDSRSTYGYLRVCAQLRREGVVVDRKTVAASMRRQG
LAGISPRRFRPVTTIPGTRTHSIPDRVKRHWDTGQVDRVWVTDITYLRTRAGWVYLCAIKDACYRKVIATAMSTTMTTDLVEEALRRARILRPNAPRKVI
IHSDRGTQFTSEQMYECCRELHLDQSMGRTGVCWDNAMIESQWSVLKAEFYDRYEWDTPQQAIQGVEEWIYDFYNTKRLNSAIGYQTPVEFEAQHAAALT
RAA
Blast result :
Comments
ISPfr11 is 65% aa similar to ISAar26. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2008)