ISPfr10

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Propionibacterium freudenreichii
Propionibacterium freudenreichii
DNA section
IS Length : 1247 bp

Ends


IR Length : 34/44

IRL : TGGGCTGACCCCGTTTTTTAGGTCCGGTGGTTCTGGGAGTCGTCCTGTCG
IRR : TGGGCCGCCCCCTTCATTCGGTCCGGTCGCTCTGTGAGTCGTCGGTTTCG

Insertion site


Left flankDirect repeatRight flankDR Length
GTAGCCAGGCCTCGGTGGCT0
CCGAACGGCTCATGCGCCAGCGGC4
ATGTCCGAAACCACCAGGGTGTGG4
CAGCCCCTCGCACTGACCAA0
AGCCGCGCTCCGCACAAGGAAGAG4
GGCTCGTGTCCTGGCGCCACACAT4
CACATCCTCGAGGCTGCCTC0
CTCGAATGGGGGGTCCCTTCTTAG4

DNA sequence

TGGGCTGACCCCGTTTTTTAGGTCCGGTGGTTCTGGGAGTCGTCCTGTCGCCCGTGGTTGCGGGCAGGGAGACTGGTCAGATCATGTCGAACAGCAGGAA
GCGTCACACCCCGGAGCAGGTCGTCCGCAAGCTCGGGCAGGCCGACAGGATGCTCGCCGACGGCAGCGACATCGCCGCGGTGTGTCGGGAGCTTGGGATA
TCCGAGCAGACGTATTACCGGTGGCGTAACCAGTACGGCGGACTGAAGGCCGACGACGCGAAGCGCCTCAGGGAGCTGGAGAAGCAGAACGCCACGCTCA
AGCGGTTGCTGGCCGAAGCGGAGCTGGAGAAGGCTGCGCTCAAGGAGCTGGCCGAGGGAAACTTCTAGGCCCGGGCAGGCGCCGCGCCGCCGTCTCGCAC
CTGATCAGGACACTGCAGGTGAGCGAGCGGATGGCGTGCCGTCTGGCCGGGCTGAGCAGGTCCGCGTACCGTCGCCCGCTCAAGGGCGACACGGTCGCCG
ACCCTGATCGCGCGTTGCGAGACTGGCTGCGCGCGTGGGCGAAGAAGCATCCCCGCTACGGGTACCGGCGGGCGTATCACGACGCCCGGGGTGAGGGCTG
GGCGGTCAACCACAAGAAGATCCAACGCCTCTGGCGAGCCGAAGGCCTCCGCGTCCCGCAGCGGCGACGCCGCAAGCGTGTCGGATCCTCGACCGTCGAC
GCCCCGGCGGCGGTCGCACCGAACCTCGTATGGGCGGTGGACTTCCAGTTCGACGCCGACGAGCGGGGCTGCCCGATCAAGATCTGCTCCATCGTCGACG
AGCACACCCGCGAATGCATCGGCGGGCTCGTGGAGCGGTCGATCACCGCCGACCGGCTCACCGCCCACCTCGAAGAGCTCGTCGCCGTCCGCGGTGCACC
GGGTGTGCTCCGCAGCGACAACGGGCCCGAGTTCATCAGCGACGCGATGGCCGACTGGGCGGGTACCCGCACCGGCCTGTTCTACATTCCGCCCGGCTCG
CCCTGGCACAACGGATACGTCGAATCGTTCAACAGCCGCCTCCGCGACGAGTGCCTCAACATCAACAGCTTCTACTCACTGCTGCACGCTCAGGTCGTGA
TCGGCGACTGGAAGACCGAGTACAACCATCAGCGTCGGCACTCATCGCTCGGCTACCTACCCCCGGCCGAGTACGCTCGGCAGTGCACCCATCAAATCGA
AACCGACGACTCACAGAGCGACCGGACCGAATGAAGGGGGCGGCCCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
285 bp94 aa84368+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSNSRKRHTPEQVVRKLGQADRMLADGSDIAAVCRELGISEQTYYRWRNQYGGLKADDAKRLRELEKQNATLKRLLAEAELEKAALKELAEGNF

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1005 bp334 aa2301234+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

PVRRTEGRRREAPQGAGEAERHAQAVAGRSGAGEGCAQGAGRGKLLGPGRRRAAVSHLIRTLQVSERMACRLAGLSRSAYRRPLKGDTVADPDRALRDWL
RAWAKKHPRYGYRRAYHDARGEGWAVNHKKIQRLWRAEGLRVPQRRRRKRVGSSTVDAPAAVAPNLVWAVDFQFDADERGCPIKICSIVDEHTRECIGGL
VERSITADRLTAHLEELVAVRGAPGVLRSDNGPEFISDAMADWAGTRTGLFYIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNH
QRRHSSLGYLPPAEYARQCTHQIETDDSQSDRTE

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1151 bp383 aa841234+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSNSRKRHTPEQVVRKLGQADRMLADGSDIAAVCRELGISEQTYYRWRNQYGGLKADDAKRLRELEKQNATLKRLLAEAELEKAALKELAEGKLLGPGRR
RAAVSHLIRTLQVSERMACRLAGLSRSAYRRPLKGDTVADPDRALRDWLRAWAKKHPRYGYRRAYHDARGEGWAVNHKKIQRLWRAEGLRVPQRRRRKRV
GSSTVDAPAAVAPNLVWAVDFQFDADERGCPIKICSIVDEHTRECIGGLVERSITADRLTAHLEELVAVRGAPGVLRSDNGPEFISDAMADWAGTRTGLF
YIPPGSPWHNGYVESFNSRLRDECLNINSFYSLLHAQVVIGDWKTEYNHQRRHSSLGYLPPAEYARQCTHQIETDDSQSDRTE

 

Blast result :
Comments
ISPfr10 is 95% aa similar to ISAar43. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] ISfinder annotation (2009)