ISCpe8

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
FJ589781 ND Clostridium perfringens
Clostridium perfringens
DNA section
IS Length : 1964 bp

Ends


IR Length : 22/24

IRL : GGCTATGTTAGCTAACTTTGTGATTAATTATATCTAAAATAGGCTATAAT
IRR : GGCTATGTTAGCTATCTTCGTGATAGATATGACATAAAAACAACCACGCC

Insertion site


Left flankDirect repeatRight flankDR Length
TACTCCCATTCATATATTATAAAATAATAAATAATATGGGAGTATTTA8

DNA sequence

GGCTATGTTAGCTAACTTTGTGATTAATTATATCTAAAATAGGCTATAATATAGGTATAGACTATATTTCAACTAATTTACAGAAAGGATACCTAATATT
ATGTCAAAAATAGATATAAAAGCTATGATAAAAGATTTAAAAAAGAATGAACTTACAGAGTTAATTTCAGTAGCACAAGAAGTATTAAGTACTTTATTTA
ATTCTTCTGAAATTAGAGATAATGTTAAAGAAAGTAGATTTTCTAAAGGATACGAGTGCCCAAAATGTCAATGTAAAGATGTAAATAAAAATGGGAAATC
TAATGGAAGACAAAGATATATATGTAAACGTTGTCGTACAAGTTTTGATGAGTTTACTATGTCCCCATTCTCTAATACAAAATTAGGGTTAGATAAATGG
ATAAAATACTGCGAATTAATGATATTAGGACTTTCTATAAGAAAATGTGCTGAAGAAGTAGGAGTAGGTGTTAAAACGTCTTTTTACATGCGTCATAGGA
TACTTGATGTAATCAATTTATCATTAAAAAATGATAAAGTTGAAGGTATAGTTGAAGTAGATGAATGCTTTATTAAGGAGTCTTTTAAAGGGAATCATTC
TAAAAGTACTACATTTGTAATGCCTAGAAATCCTAGAAAAAGAGGTAAAGGTAAAAATGATAAGAAGAAAAGAGGAATATCAAAAGAGCAGATTTGCATA
GAGACAGCAATTGATAGAAAAGGAAATATCCTTATGAGTGCTGTTTGTAATGGCAGAATTACAACAAATCAAATAATTAACTTCTTTGACAATAAAATAT
GTGAAGATGCTACTTTTTGCGTAGATTCACATAAATCATATATGGCAATAAAGGATAAGTTGAATATAGAGTTAAAGCAAGTTCCTAGGGGAAAATCAAT
GATAGATAGTGTTTATCATTTACAGCATATAAATGCTCTTCACAGTAGCTTTAAGAGATGGTTAATGACTTTTAATGGTGTATCCACAAAATATATCAAT
AATTATTTGGCTTGGTTTAAATTTCTACAACTAAGTAAGAAAAATAAAAAGAATGACCGAATTAAAGATATGCTAGTGAATGTAGCTACTAAGGATACTT
ATGTAACTAGAGCCACTATTAGAAATAGATTCATTGAGTTAATATAAAATAAGGAACTTTACTTAGAATTAATGAAACCATTTTATAATTGGAAAATAAT
CGAATACTTTGTATAATATAATTAAGTTCACAATTGTTATATAATGTGAAGTGAAGATATTATTAATATTATATATAAATAGAAATTTCTAGAGGTGATA
AAGTGATTGGAATAAATGATGCTTGTGAAATATTGAGTTGGGCATATAATAATAATATAGAAATATGGTTAGATGGTGGTTGGGGTGTAGATGCTTTACT
TGGAAAAGAAACACGTCAGCATAATGACATTGATTTATTTGTAGAAGAAAAAAACTATAATAAATTTATTGAAATAATTAAAAATAAAGGATTTAATGAA
ATTGTAGTGGAGTATACAAGTGAAGTGCATACTATTTGGTCTGATAATAAATTACGAATTATTGATTTACACATGTTTAAAGATAACTGTGATGGAACCA
TATGTTATGAAGGCGAAGTTTTTCAAAAAAATATATTCGATGGTGTTGGAAAAATAGGAAATATTATGGTTTCTTGTATAAATGCTAAAAATCAAGTCTT
ATTTCACTTAGGGTATGAATTTGGAGAAAGTGATATTCATGATGTAAAATTATTATGTAAAGAATTTAACATTCCGATACCTAAAGAATATGAAAATTTT
TAAATTAGCGATAATTATAATCTTTAGATTTAATAATATTACTATATCACGAAGAATTTATAATAAGTCATAAAAACAACCACATCTTATAATTCAATGT
ATTGAAAAATCTAAGGCGTGGTTGTTTTTATGTCATATCTATCACGAAGATAGCTAACATAGCC
Protein section
ORF number : 2

 

ORF 2
LengthBeginEndStrandFusion ORF
501 bp166 aa13031803+No
ORF function : Passenger Gene
Annotation : O-lincosamide nucleotidyltransferaseDescription : Antibiotic resistance

ORF sequence :

MIGINDACEILSWAYNNNIEIWLDGGWGVDALLGKETRQHNDIDLFVEEKNYNKFIEIIKNKGFNEIVVEYTSEVHTIWSDNKLRIIDLHMFKDNCDGTI
CYEGEVFQKNIFDGVGKIGNIMVSCINAKNQVLFHLGYEFGESDIHDVKLLCKEFNIPIPKEYENF

 

Blast result :
ORF 1
LengthBeginEndStrandFusion ORF
1047 bp348 aa1011147+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSKIDIKAMIKDLKKNELTELISVAQEVLSTLFNSSEIRDNVKESRFSKGYECPKCQCKDVNKNGKSNGRQRYICKRCRTSFDEFTMSPFSNTKLGLDKW
IKYCELMILGLSIRKCAEEVGVGVKTSFYMRHRILDVINLSLKNDKVEGIVEVDECFIKESFKGNHSKSTTFVMPRNPRKRGKGKNDKKKRGISKEQICI
ETAIDRKGNILMSAVCNGRITTNQIINFFDNKICEDATFCVDSHKSYMAIKDKLNIELKQVPRGKSMIDSVYHLQHINALHSSFKRWLMTFNGVSTKYIN
NYLAWFKFLQLSKKNKKNDRIKDMLVNVATKDTYVTRATIRNRFIELI

 

Blast result :
Comments
tISCpe8 is 99% (ORFA : the transposase) aa similar to ISCba1. The second ORF is a passenger gene annotated as lincosamide nucleotidyltransferase (lnuP).
References
1] Julian Roods (2009) Direct submission.
2] Dena Lyras , Vicki Adams1, Susan A. Ballard, Wee L. Teng, Pauline M. Howarth, Paul K. Crellin , Trudi L. Bannam, J. Glenn Songer and Julian I. Rood. (2009) J. Bact