ISTsi3

  • Family IS200/IS605
  • Group IS605
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_012883 ND Thermococcus sibiricus
Thermococcus sibiricus MM 739
DNA section
IS Length : 1883 bp

Ends


Left end : TTTTTCTTTTGAATGCCTTGTCCTTTAGGGCGGGGATGCAGTAATTCCAAAACAAGTCCTCCACCTATGAAAGCGAAAAACATTTAAACCTCAAGTGCGT II struct. : Yes
Right end : CCCCACTTTATCTTGGGTCCGAATGAGACCCCTCAAACCTCCCCGTCAATGGCGAGGGATTAAGCCGAACCCTCGCCCTTCAGGGCGGGGAGGAGGTCAG II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
CTCATCCTTCTTTACTTTGAAATCTCTtcag

DNA sequence

TTTTTCTTTTGAATGCCTTGTCCTTTAGGGCGGGGATGCAGTAATTCCAAAACAAGTCCTCCACCTATGAAAGCGAAAAACATTTAAACCTCAAGTGCGT
ACCATACATTAGAGGGTTCAATATGGAGACCAGAATACCAGCATTTAGCTCAACGAGACACGTAAAACACTTCCTCGCCTACCACTTCGTATGGATACCA
AAATACCGAAGGGACATCCTCACCGGGAAAGTCGCTGAAAGATTAAAACAAATGCTCAAAGAATTCGCCGGAGAAATCGGGTGTGAAGTGATCTCCCTTG
AAGTAATTCCCGACCACGTTCACGTCTTCCTCAGAGCAAAACCCGACCTCGCCCCAGCAAGAATAATCAACCACCTGAAGGGGAAGAGTGCAAGAAAACT
CCTCCAAGAATTTCCAGAATTGAGAACAAAAACTGCCCACGGGAGGTTATGGTCACGCTCCTATTTTGTAGCCTCGGCCGGATACATAACCGACGAGATC
GTAAAACACTACGTAGAAACCCAATGGGAGCGTGAGTTAAAACGAAGAGGACAGTAAGGATAAAACTCCAGCCCTCAAAAGAACAAGAGAAAGCCCTCTT
CGAGCTAGCTGACGCTGGTGCCAAAGTCTGGAATGAAGTGAATTACCTTCGCAGGCAACAGTTCTTCAACCACGAACCTGTTGATTTTAACAGGACTGAA
AAAATCGTTTATGAGAAGTACAAGGGTGAGATTGGCTCTGCGACAGTTCAGCAGATAGCGAGGAAGAACGCTGAAGCTTGGAGGAGCTTCTTCTCCCTCA
TAAAGAAGCGAAAAGAACTTCCCAAGTGGCTGAGGCCAAAACCACCGAACTACCTGAAAGAAGAGGGAAAGAGGAAGCCCTTAATTGTCCTCAGGAACGA
CCAGTACAGGATTGATGGGAACAAGGTAATTCTCAAGGGGCTTGGGAAATTCAAAAAGCTGGAAGTCCAGTTTAAGGGGCGGATACACCTGAAAGGCAAG
CAAGGGCGCTTGGAAATCATCTACGACGACGTGAAGAGGAAGTGGTACGCCCACATCAGCTACACCGTCGAAGAGAAGCTGGAAGGCAATTCTTGGGTTA
AACTCCCAAGAACGCCGAAAGGCAACCTCGTGGCTGGCATTGACCTAGGAGTGAACAATTTAATGGCCGTTTACGTTGAGAATGGGGAGAGTTTTCTGGT
CAATGGTAGGCCGTTGAAGAGCATTGGCTTTTACTGGCAGAAGAGGATTGCTGAGTATCAGTCAAAACTCAACAAGAGTGGGGCTAAGGCGAGCAGAAAG
CTCAAGAGAATGCACGAGAAGGCAAAACTCCAAGCGGGACACTACATTAACACCGCAGTCAGGCAGACGGTTAGGAGGCTTTACGAGCTGGGAGTTTCGA
GGATTGTAGTTGGCTATCCAAAGGAGATTTCAAGAGAACCAGACAAGGGCAGAAAGCAGAATTTCGTCCTTTCTCACGTTTGGCGGTTTAATTACGTGAT
TAAACGTTTAACAGAGGTTGCTGAGGAGTATGGTATTCAGGTCGTGGTTGTGAATGAGGCTTTCACTTCTCAGACGTGCCCTCTCTGCGGGAAGCCTCAT
AAAGGGGCGAGGTTTGTCCGTGGGCTGTATATGTGTCCCGTGGAGGGGCTTGTGTTCAATGCTGATTTAGTTGGTGCTTTCAACATTTTGAGGAAGGCCG
TGAAAACGATAACCCCGAATCTGAGCGGTCTTTATGCTCAGGGGAGGGGTAACGGGCCTGAGACCGGGCCAGAGGGGTTGAAGCCCCACTTTATCTTGGG
TCCGAATGAGACCCCTCAAACCTCCCCGTCAATGGCGAGGGATTAAGCCGAACCCTCGCCCTTCAGGGCGGGGAGGAGGTCAG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
435 bp144 aa123557+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

METRIPAFSSTRHVKHFLAYHFVWIPKYRRDILTGKVAERLKQMLKEFAGEIGCEVISLEVIPDHVHVFLRAKPDLAPARIINHLKGKSARKLLQEFPEL
RTKTAHGRLWSRSYFVASAGYITDEIVKHYVETQWERELKRRGQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1304 bp434 aa5431846+No
ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MKRTVRIKLQPSKEQEKALFELADAGAKVWNEVNYLRRQQFFNHEPVDFNRTEKIVYEKYKGEIGSATVQQIARKNAEAWRSFFSLIKKRKELPKWLRPK
PPNYLKEEGKRKPLIVLRNDQYRIDGNKVILKGLGKFKKLEVQFKGRIHLKGKQGRLEIIYDDVKRKWYAHISYTVEEKLEGNSWVKLPRTPKGNLVAGI
DLGVNNLMAVYVENGESFLVNGRPLKSIGFYWQKRIAEYQSKLNKSGAKASRKLKRMHEKAKLQAGHYINTAVRQTVRRLYELGVSRIVVGYPKEISREP
DKGRKQNFVLSHVWRFNYVIKRLTEVAEEYGIQVVVVNEAFTSQTCPLCGKPHKGARFVRGLYMCPVEGLVFNADLVGAFNILRKAVKTITPNLSGLYAQ
GRGNGPETGPEGLKPHFILGPNETPQTSPSMARD

 

Blast result :
Comments
ISTsi3 is 77% (ORFA: the transposase) and 66% (ORFB) aa similar to ISDka2.
References
1] Mardanov A.V., Ravin N.V., Svetlitchnyi V.A., Beletsky A.V., Miroshnichenko M.L., Bonch-Osmolovskaya E.A., Skryabin K.G. (2009) Appl. Environ. Microbiol., 75, 4580-4588.