ISfinder Mirror Site

ISKol3

Family IS21
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_012785	ND	Kosmotoga olearia	Kosmotoga olearia TBF 19.5.1

DNA section

IS Length : 2374 bp

Ends

IR Length : 29/37

IRL : TGGCAGGATAAAAATCCAGCGGTAAGGGAAAGAAAAAGATCATTGCCAGC
IRR : TGGCAATATAAAAATCCAGCGTTTGGAAAAATAAAAAACCAGCACCCGGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATACAGCAAG		AAAACATTCT	0
AATACAGCAAG		AAAACAGACC	0
GATACAGCGAG		AAAACAATAG	0
AATACAGCAAG		AAAACACTCA	0

DNA sequence

TGGCAGGATAAAAATCCAGCGGTAAGGGAAAGAAAAAGATCATTGCCAGCATCCGGAAAATCCATTACCCTTTAATTGCACACCAAAACAGTTAAAGGGG
GTTAGAAAGGAGTGCTGACAATGATCCAGGTACATCATATCAAATATCTACGTGAATTTAAAGGGAAGTCGCTAAGAAATATAGCCAACGAAACTGGCCA
CGATTTCAGAACGGTGAAGAAATATGACGAAATGGATGATTTCAACGAGTATGTTCAACGTAGAAAGAGGACTTCCAAGCTCGATCCGTACAAGCCCAAA
ATAGATGAATGGCTGGAAGAAGATAAAAAGGTAAAGGCAAAACAACGTCATACTGCTAGAAGGATTTACAAGAGACTATGTGAGGAATATCCGAATTTTC
CGCTCAGCGAACGTACGGTAAGAGCATATGTGAGAAAGAAAAAGAAGGAATTGTATTCCAACGAAGGTTATTTACCTCTTGACCATCCTGGTGGTGAAGC
TCAAGCAGATTTTGGACAGGCTGAGTTCATTGAAAAGGGTAAGGGAACGACGCTGCATTATCTGAATCTTTCTTTTCCATACAGCAACGGGGGATACTTT
CAACTGTTTAGAGGGGAAAACCTTGAATGTTTGCTCGAGGGGCTGAAGAACATATTCGAACATATTGGAAAGGTTCCTCGGCGTATATGGTTCGACAACC
TATCTCCCGTGGTGAAAAAAATTCTGGATGGAGGGGAAAGGGAGGTAACGGAGAAGTTTTACAGATTCTGTCAACATTATGGTTTTGAACCTGTGTTTTG
TAATCCTGGTTGTGGCAATGAAAAAGGCAATGTGGAAAACAAGGTTGGATATAACAGGCGGAACTTCTTTGTTCCCATACCCAGGATAGAGAATCTTGAA
GATTACAACAAAGAGCTCTTAATCCTCTCGGAGAAAGATATGGAAAGACCCCATTACCGAAAGGAAAAGAGCATTAAAGAACTGTTTTTAGAAGAAAAGA
AAAAGATGCTCCTACTCAACGAAAAACCTCTGGATATTTCCAGGGTACAGAAAGCAAAGACAGACAAGTATGGGATGCTTCGTTTTGAAAACAACAGATA
CTCCGTGTCACCAAAACATACTGAAACAGAAGTGTGGTTGAAGATAGGGGCATCGGAAATAGAGATACTGGATGAAGACTACAAAGTGCTCGTAAAACAT
CCCAGGTTATACGGACACAACCTCCAATCTCTGAATTGGTATCCATACCTTGAGACATTAAGCAGAAGACCGCGTGCGTTGAAATACACAGGATTTTACA
GGGAATTATCTCCTATTTGGCAAGAATACTTTGAATCCTGTGATTACCAGGAAAAGAAAAACAGCCTGAAATTGCTGGTCAAAATGCTACGTGAAACAGA
TATGGAAACCGCGAGATATGCACTGGAGATAAGCCAGAATAGTGAACGTTGCACTGCTGAGACGATATTCCTATCGTACCGACGGTTGATCCAGGAAGAA
CCATCACCTTTTGAAACAGGGAACGAAGTACCGAGATTACAAGCATATACAACCGATTTTAAAGAATACGATGCCCTGGTGGGTGAAAGGAAATGAAAGA
ACTCATCGCTCAATACTGCAAAGAACTGAGATTGGGAAAAAGTATAGTGGAAAACTACCAAAAGATACAGGCAGAAACCAACGAGGAATTCCTGGTGAAA
CTACTGAAGCTGGAGATTGAAAACAGACGGGTATCACGTAAAAATCGTTATCTAAAACAGGCGAATTTCGAGGTAATGAAAACCTTCGAAGACTACAGTT
TTGAAAACGTACAGATACCCAAGAGTATAACATTGGAAGAATTACAAGAAGGGAGGTTTCTGGGAAAGAAAGAGAACCTAATACTGTATGGCCCTGTAGG
AACAGGAAAAACACACATGGCAACAGCCATAGGTATCGCAGCTTGTAACCGGGAAAAGAAAGTCAGATTTTACAGAACGGCCACACTGGTAAATGAACTT
GTGGAAGCAAAAAGCAACGGAACATTGAAGAGGTTCTTGAAGACACTCAGAAAGACAGATTTGCTCATATGCGACGAATGGGGTTACATACCTTTAGACC
GGGAAGGAGCGCAACTCTTGTTTCAAGTGATTGCGGAAAGATATGAGAGGAACAGCGTAATAATCACAACGAACCTTGAGTTCAGCAAATGGAATGGGAT
ATTCTACGATGAGAAACTCACCAGTGCCATAATAGACAGATTGATTCACCATTGCCATTTATTCGTATTTGCAGGCAAGAGTTACCGATTGGAACATTCG
AGTATTAAGACTTAATTTTTTTACCCCGGGTGCTGGTTTTTTATTTTTCCAAACGCTGGATTTTTATATTGCCA

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
1476 bp	491 aa	121	1596	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MIQVHHIKYLREFKGKSLRNIANETGHDFRTVKKYDEMDDFNEYVQRRKRTSKLDPYKPKIDEWLEEDKKVKAKQRHTARRIYKRLCEEYPNFPLSERTV
RAYVRKKKKELYSNEGYLPLDHPGGEAQADFGQAEFIEKGKGTTLHYLNLSFPYSNGGYFQLFRGENLECLLEGLKNIFEHIGKVPRRIWFDNLSPVVKK
ILDGGEREVTEKFYRFCQHYGFEPVFCNPGCGNEKGNVENKVGYNRRNFFVPIPRIENLEDYNKELLILSEKDMERPHYRKEKSIKELFLEEKKKMLLLN
EKPLDISRVQKAKTDKYGMLRFENNRYSVSPKHTETEVWLKIGASEIEILDEDYKVLVKHPRLYGHNLQSLNWYPYLETLSRRPRALKYTGFYRELSPIW
QEYFESCDYQEKKNSLKLLVKMLRETDMETARYALEISQNSERCTAETIFLSYRRLIQEEPSPFETGNEVPRLQAYTTDFKEYDALVGERK

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
723 bp	240 aa	1593	2315	+	No

ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MKELIAQYCKELRLGKSIVENYQKIQAETNEEFLVKLLKLEIENRRVSRKNRYLKQANFEVMKTFEDYSFENVQIPKSITLEELQEGRFLGKKENLILYG
PVGTGKTHMATAIGIAACNREKKVRFYRTATLVNELVEAKSNGTLKRFLKTLRKTDLLICDEWGYIPLDREGAQLLFQVIAERYERNSVIITTNLEFSKW
NGIFYDEKLTSAIIDRLIHHCHLFVFAGKSYRLEHSSIKT

Blast result :

Comments

ISKol3 is 65% (ORFA : the transposase) and 82% (istB : the helper of transposition) aa similar to ISCth15.

References

1] ISfinder annotation (2009)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Chertkov,O., Brettin,T., Detter,J.C., Han,C., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ovchinnikova,G. and Noll,K. (2009) Direct submission GenBank.