ISAar37
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Arthrobacter arilaitensis | Arthrobacter arilaitensis RE117 |
DNA section
IS Length : 1309 bp
Ends
IR Length : 18/25
IRL : TGAGCTGTCATGATGTTGTGAGACACGCGTATGTAGGAGAATGAGTTCAT
IRR : TGAGCTGGTCAAGTTTCTGAGACACGAACTTTGATTTTTTCTTATTAAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CGTAAGGTTA | TTG | AGCAGGGGTA | 3 |
DNA sequence
TGAGCTGTCATGATGTTGTGAGACACGCGTATGTAGGAGAATGAGTTCATGTCCAAACGTCAGAAATTCACCCCGGAATTCCGTGCAGAAGCGGTGGAAC
TGGTGCTTTCCTCAGGAAAAAGCCTGGTCGAGGTTTCAGAATCACTGGGAATCAACGAAGGCACCCTTGGGAACTGGGTTCGCGCCTACCGAACCGAGCA
TCCAGAGACCGAAACCGAGGAACGCGGACCGGTGGAATGGGCCCAGCACGAGAAGCTGCGCCGGGAATTGGCGGAGGTGAAACGGGAGAACGAATTTTTA
AAAGATGTCAGCGCCTATTTCGCCTCGAAGGGCAAGAAATAAGCGCGATCTTCCGATTCATCATGGAACACAGCGCGAAGTATCCGGTGAACTGGATGTG
CCAGCAGCTAGAGGTCTCGCGCGCTTCGTATTACCGGTGGTTGAACCCGTCCACTGAGCTCAGCGAAGCCAAGGCCCTGCGGGCTATGCGCACCGTTCTG
GTCCTGACCGAGTACGAGGCTTCCAACGGGGTGGCCGGTGCCGGCCAATTAGTGGATTTGATTCGCAACAACCACAAGCAGTACGTGCCGAAATCCACGG
TGCTGTCGATCATGAACGAGCACGGGCTCAAGGCTAAACGAGTGAGTGCTTTCAAAGTCACCACGGTGCATGACCAGCAAGCCAGGACCGCTCATATCAA
GAATCACATGGTCGATAAGCTCGGCCGCCGTGATTTTCGTTCGTCCCGACCTGGAACCAAGTTGGTGGGCGATATTACCTACTTGCGAACCGGTGAAGGG
TGGCTGTATCTGGCGACGGTGATCGACTTGTATTCACGTCAGATCGTTGGGTGGTCAATGGGTGCGAACATGCAAACGCAACTGGTTATCGATGCGATGC
AGCTCGCGATGTCGCGTGGTTTCATCAGCGCTGGGACTGTTTTTCATTCAGACCGCGGAACTCAGTACACGTCAGATGCATTTCAAGCGTGGTGCTCTGA
GAATGGTGTGCGGCAGTCAATGGGCCGTACCGGAGTGTGTTGGGACAATGCCGTTGCTGAGTCGGCGTTCTCGACGATCAAGAACGAGATGTACCATCAT
CGGGCGTTTACGAGCCGGGTTGCGGCGCGTACCGCGGTTATGGAGTTCATTGAGATTTGGTACAACAGGAAGCGTCCGCATACGACTAATGGCGGGTTGA
GTCCTCAGATGTTGCTCGACTGGTATTGGGAGCAGAATTCTTTGGCTCTCGCGGCCTGAGTTTAATAAGAAAAAATCAAAGTTCGTGTCTCAGAAACTTG
ACCAGCTCA
TGGTGCTTTCCTCAGGAAAAAGCCTGGTCGAGGTTTCAGAATCACTGGGAATCAACGAAGGCACCCTTGGGAACTGGGTTCGCGCCTACCGAACCGAGCA
TCCAGAGACCGAAACCGAGGAACGCGGACCGGTGGAATGGGCCCAGCACGAGAAGCTGCGCCGGGAATTGGCGGAGGTGAAACGGGAGAACGAATTTTTA
AAAGATGTCAGCGCCTATTTCGCCTCGAAGGGCAAGAAATAAGCGCGATCTTCCGATTCATCATGGAACACAGCGCGAAGTATCCGGTGAACTGGATGTG
CCAGCAGCTAGAGGTCTCGCGCGCTTCGTATTACCGGTGGTTGAACCCGTCCACTGAGCTCAGCGAAGCCAAGGCCCTGCGGGCTATGCGCACCGTTCTG
GTCCTGACCGAGTACGAGGCTTCCAACGGGGTGGCCGGTGCCGGCCAATTAGTGGATTTGATTCGCAACAACCACAAGCAGTACGTGCCGAAATCCACGG
TGCTGTCGATCATGAACGAGCACGGGCTCAAGGCTAAACGAGTGAGTGCTTTCAAAGTCACCACGGTGCATGACCAGCAAGCCAGGACCGCTCATATCAA
GAATCACATGGTCGATAAGCTCGGCCGCCGTGATTTTCGTTCGTCCCGACCTGGAACCAAGTTGGTGGGCGATATTACCTACTTGCGAACCGGTGAAGGG
TGGCTGTATCTGGCGACGGTGATCGACTTGTATTCACGTCAGATCGTTGGGTGGTCAATGGGTGCGAACATGCAAACGCAACTGGTTATCGATGCGATGC
AGCTCGCGATGTCGCGTGGTTTCATCAGCGCTGGGACTGTTTTTCATTCAGACCGCGGAACTCAGTACACGTCAGATGCATTTCAAGCGTGGTGCTCTGA
GAATGGTGTGCGGCAGTCAATGGGCCGTACCGGAGTGTGTTGGGACAATGCCGTTGCTGAGTCGGCGTTCTCGACGATCAAGAACGAGATGTACCATCAT
CGGGCGTTTACGAGCCGGGTTGCGGCGCGTACCGCGGTTATGGAGTTCATTGAGATTTGGTACAACAGGAAGCGTCCGCATACGACTAATGGCGGGTTGA
GTCCTCAGATGTTGCTCGACTGGTATTGGGAGCAGAATTCTTTGGCTCTCGCGGCCTGAGTTTAATAAGAAAAAATCAAAGTTCGTGTCTCAGAAACTTG
ACCAGCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 294 bp | 97 aa | 49 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MSKRQKFTPEFRAEAVELVLSSGKSLVEVSESLGINEGTLGNWVRAYRTEHPETETEERGPVEWAQHEKLRRELAEVKRENEFLKDVSAYFASKGKK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1023 bp | 340 aa | 237 | 1259 | + | No |
Description : Second part of the transposase
ORF sequence :
MGPAREAAPGIGGGETGERIFKRCQRLFRLEGQEISAIFRFIMEHSAKYPVNWMCQQLEVSRASYYRWLNPSTELSEAKALRAMRTVLVLTEYEASNGVA
GAGQLVDLIRNNHKQYVPKSTVLSIMNEHGLKAKRVSAFKVTTVHDQQARTAHIKNHMVDKLGRRDFRSSRPGTKLVGDITYLRTGEGWLYLATVIDLYS
RQIVGWSMGANMQTQLVIDAMQLAMSRGFISAGTVFHSDRGTQYTSDAFQAWCSENGVRQSMGRTGVCWDNAVAESAFSTIKNEMYHHRAFTSRVAARTA
VMEFIEIWYNRKRPHTTNGGLSPQMLLDWYWEQNSLALAA
GAGQLVDLIRNNHKQYVPKSTVLSIMNEHGLKAKRVSAFKVTTVHDQQARTAHIKNHMVDKLGRRDFRSSRPGTKLVGDITYLRTGEGWLYLATVIDLYS
RQIVGWSMGANMQTQLVIDAMQLAMSRGFISAGTVFHSDRGTQYTSDAFQAWCSENGVRQSMGRTGVCWDNAVAESAFSTIKNEMYHHRAFTSRVAARTA
VMEFIEIWYNRKRPHTTNGGLSPQMLLDWYWEQNSLALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1211 bp | 403 aa | 49 | 1259 | + | Yes |
Chemistry : DDE
ORF sequence :
MSKRQKFTPEFRAEAVELVLSSGKSLVEVSESLGINEGTLGNWVRAYRTEHPETETEERGPVEWAQHEKLRRELAEVKRENEFFKRCQRLFRLEGQEISA
IFRFIMEHSAKYPVNWMCQQLEVSRASYYRWLNPSTELSEAKALRAMRTVLVLTEYEASNGVAGAGQLVDLIRNNHKQYVPKSTVLSIMNEHGLKAKRVS
AFKVTTVHDQQARTAHIKNHMVDKLGRRDFRSSRPGTKLVGDITYLRTGEGWLYLATVIDLYSRQIVGWSMGANMQTQLVIDAMQLAMSRGFISAGTVFH
SDRGTQYTSDAFQAWCSENGVRQSMGRTGVCWDNAVAESAFSTIKNEMYHHRAFTSRVAARTAVMEFIEIWYNRKRPHTTNGGLSPQMLLDWYWEQNSLA
LAA
IFRFIMEHSAKYPVNWMCQQLEVSRASYYRWLNPSTELSEAKALRAMRTVLVLTEYEASNGVAGAGQLVDLIRNNHKQYVPKSTVLSIMNEHGLKAKRVS
AFKVTTVHDQQARTAHIKNHMVDKLGRRDFRSSRPGTKLVGDITYLRTGEGWLYLATVIDLYSRQIVGWSMGANMQTQLVIDAMQLAMSRGFISAGTVFH
SDRGTQYTSDAFQAWCSENGVRQSMGRTGVCWDNAVAESAFSTIKNEMYHHRAFTSRVAARTAVMEFIEIWYNRKRPHTTNGGLSPQMLLDWYWEQNSLA
LAA
Blast result :
Comments
ISAar37 is 91% aa similar to ISAar25.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2009).